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[1]溫博智,陳月峰,王寶劍,等.腸道菌群與頸椎病因果關(guān)系的雙向孟德爾隨機化分析[J].中醫(yī)正骨,2024,36(12):43-48.
　WEN Bozhi,CHEN Yuefeng,WANG Baojian,et al.Investigation on the causal relationship between gut microbiota and cervical spondylosis:a bidirectional mendelian randomization analysis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2024,36(12):43-48.
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腸道菌群與頸椎病因果關(guān)系的雙向孟德爾隨機化分析()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第36卷
期數(shù):
2024年12期
頁碼:
43-48
欄目:
數(shù)據(jù)庫研究
出版日期:
2024-12-20

文章信息/Info

Title:
Investigation on the causal relationship between gut microbiota and cervical spondylosis:a bidirectional mendelian randomization analysis
作者:
溫博智; 陳月峰; 王寶劍; 馬睿辰; 戴文康; 馬玉峰
北京中醫(yī)藥大學(xué)第三附屬醫(yī)院,北京 100029
Author(s):
WEN Bozhi; CHEN Yuefeng; WANG Baojian; MA Ruichen; DAI Wenkang; MA Yufeng
The Third Affiliated Hospital of Beijing University of Traditional Chinese Medicine,Beijing 100029,China
關(guān)鍵詞:
頸椎病;  痹證;  脾(中醫(yī));  腸道微生物群;  孟德爾隨機化分析
Keywords:
cervical spondylosis;  arthromyodynia;  spleen(TCM);  gastrointestinal microbiome;  Mendelian randomization analysis
摘要:
目的:探討腸道菌群與頸椎病的因果關(guān)系。方法:分別從IEU OpenGWAS project數(shù)據(jù)庫和MiBioGen數(shù)據(jù)庫獲取頸椎病和腸道菌群的全基因組關(guān)聯(lián)研究數(shù)據(jù),從中篩選符合要求的單核苷酸多態(tài)性(single nucleotide polymorphism,SNP)位點作為工具變量進行兩樣本雙向孟德爾隨機化(Mendelian randomization,MR)分析。采用逆方差加權(quán)法(inverse variance weighted,IVW)作為主要分析方法,并采用MR-Egger、加權(quán)中位數(shù)法、加權(quán)模式、簡單模式進行補充分析。采用Cochran's Q檢驗對IVW和MR-Egger法的分析結(jié)果進行異質(zhì)性檢驗,采用MR-Egger回歸進行水平多效性檢驗,采用留一法進行敏感性分析。結(jié)果:共篩選出頸椎病的SNP位點70個; 腸道菌群的SNP位點46個,其中δ-變形菌綱11個、腸桿菌科8個、瘤胃球菌屬UCG011 7個、狹義梭菌屬4個、糞球菌屬8個、瘤胃梭菌屬8個。正向MR分析結(jié)果顯示,δ-變形菌綱與頸椎病存在負向因果關(guān)系,狹義梭菌屬、糞球菌屬與頸椎病存在正向因果關(guān)系; 反向MR分析結(jié)果顯示,頸椎病與腸道菌群之間不存在明顯的因果關(guān)系。水平多效性檢驗和異質(zhì)性檢驗結(jié)果顯示,不存在水平多效性和異質(zhì)性; 留一法分析結(jié)果顯示,無明顯離群值,提示MR分析結(jié)果穩(wěn)健。結(jié)論:腸道菌群中的δ-變形菌綱、狹義梭菌屬、糞球菌屬與頸椎病存在可靠的因果關(guān)系。
Abstract:
Objective:To investigate the causal relationship between gut microbiota(GM)and cervical spondylosis(CS).Methods:The genome-wide association study(GWAS)datasets about GM and CS were extracted from the IEU OpenGWAS project database and MiBioGen database,respectively.The eligible single nucleotide polymorphism(SNP)loci were screened as instrumental variables for two-sample bidirectional mendelian randomization(MR)analysis.Inverse variance weighted(IVW)were selected as the main analysis method,and MR-Egger,weighted median estimator(WME),weighted mode(WM)and simple mode(SM)as supplemental analysis method.Cochran's Q test was performed on the heterogeneity of the results analyzed by IVW method and MR-Egger method.In addition,the horizontal pleiotropy was examined by MR-Egger regression,and the sensitivity of the MR analysis results was evaluated by leave-one-out(LOO)test.Results:Seventy CS SNP loci and 46 GM(11 δ-proteobacteria,8 enterobacteriaceae,7 ruminococcus UCG011,4 clostridium sensu stricto,8 coprococcus,and 8 ruminal clostridium)SNP loci were included and served as the instrumental variables.The results of forward MR analysis showed an inverse causal relationship between δ-proteobacteria and CS,and a positive causal relationship between clostridium sensu stricto,coprococcus and CS.The results of reverse MR analysis showed that there was no obvious causal relationship between CS and GM.The results of horizontal pleiotropy test and heterogeneity test indicated that there was no horizontal pleiotropy and heterogeneity.The results of LOO test showed no outlier,suggesting the results of the MR analysis in this study were stable and reliable.Conclusion:GM,including δ-proteobacteria,clostridium sensu stricto and coprococcus,has a reliable causal relationship with CS.

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