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[1]于蘭,解冬千,王霞,等.蒺藜皂苷對(duì)白細(xì)胞介素-1β誘導(dǎo)的軟骨細(xì)胞凋亡和炎癥因子分泌的影響[J].中醫(yī)正骨,2024,36(01):23-32.
　YU Lan,XIE Dongqian,WANG Xia,et al.Effects of tribulus terrestris saponins on interleukin-1β-induced chondrocyte apoptosis and inflammatory factor secretion[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2024,36(01):23-32.
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蒺藜皂苷對(duì)白細(xì)胞介素-1β誘導(dǎo)的軟骨細(xì)胞凋亡和炎癥因子分泌的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第36卷
期數(shù):
2024年01期
頁碼:
23-32
欄目:
基礎(chǔ)研究
出版日期:
2024-01-20

文章信息/Info

Title:
Effects of tribulus terrestris saponins on interleukin-1β-induced chondrocyte apoptosis and inflammatory factor secretion
作者:
于蘭; 解冬千; 王霞; 及超
保定市第二醫(yī)院,河北 保定 071001
Author(s):
YU Lan; XIE Dongqian; WANG Xia; JI Chao
The No.2 Hospital of Baoding,Baoding 071001,Hebei,China
關(guān)鍵詞:
骨關(guān)節(jié)炎;  軟骨細(xì)胞;  細(xì)胞凋亡;  蒺藜;  皂苷類;  白細(xì)胞介素-1β;  RNA; 環(huán)狀;  炎癥介導(dǎo)素類
Keywords:
osteoarthritis;  chondrocytes;  apoptosis;  fructus tribuli;  saponins;  interleukin-1 beta;  RNA; circular;  inflammation mediators
摘要:
目的:觀察蒺藜皂苷對(duì)白細(xì)胞介素-1β(interleukin-1β,IL-1β)誘導(dǎo)的軟骨細(xì)胞凋亡和炎癥因子分泌的影響,并探討其作用機(jī)制。方法:①軟骨細(xì)胞培養(yǎng)和轉(zhuǎn)染。采用DMEM培養(yǎng)液培養(yǎng)小鼠軟骨細(xì)胞(ATDC5細(xì)胞),培養(yǎng)后采用轉(zhuǎn)染試劑分別轉(zhuǎn)染pcDNA-circ_0045714、pcDNA、si-circ_0045714、si-NC。②蒺藜皂苷濃度篩選。將ATDC5細(xì)胞接種于96孔板中,一組正常培養(yǎng)(正常組),一組加入10 ng·mL-1的IL-1β(IL-1β組),其他組在加入10 ng·mL-1 IL-1β的基礎(chǔ)上分別加入濃度為25 μg·mL-1、50 μg·mL-1、100 μg·mL-1、200 μg·mL-1、400 μg·mL-1的蒺藜皂苷。培養(yǎng)后計(jì)算軟骨細(xì)胞存活率,并確定后續(xù)實(shí)驗(yàn)蒺藜皂苷的濃度。③軟骨細(xì)胞增殖抑制率檢測(cè)。將ATDC5細(xì)胞分為對(duì)照組、IL-1β組、IL-1β+蒺藜皂苷低劑量組、IL-1β+蒺藜皂苷中劑量組、IL-1β+蒺藜皂苷高劑量組,其中對(duì)照組采用常規(guī)培養(yǎng)液培養(yǎng),IL-1β組采用含10 ng·mL-1的IL-1β培養(yǎng)液培養(yǎng),IL-1β+蒺藜皂苷低、中、高劑量組分別采用含50 μg·mL-1、100 μg·mL-1、200 μg·mL-1的蒺藜皂苷和10 ng·mL-1的IL-1β培養(yǎng)液培養(yǎng)。轉(zhuǎn)染pcDNA-circ_0045714、pcDNA的ATDC5細(xì)胞,培養(yǎng)方法同IL-1β組,并分別標(biāo)記為IL-1β+pcDNA-circ_0045714組、IL-1β+pcDNA組。轉(zhuǎn)染si-circ_0045714、si-NC的ATDC5細(xì)胞,培養(yǎng)方法同IL-1β+蒺藜皂苷高劑量組,并分別標(biāo)記為IL-1β+蒺藜皂苷高劑量+si-circ_0045714組、IL-1β+蒺藜皂苷高劑量+si-NC組。培養(yǎng)后計(jì)算軟骨細(xì)胞增殖抑制率。④軟骨細(xì)胞凋亡率檢測(cè)。于6孔板中接種ATDC5細(xì)胞及轉(zhuǎn)染pcDNA-circ_0045714、pcDNA、si-circ_0045714、si-NC的ATDC5細(xì)胞,培養(yǎng)后采用流式細(xì)胞儀檢測(cè)各組軟骨細(xì)胞凋亡率。⑤軟骨細(xì)胞中腫瘤壞死因子-α(tumor necrosis factor-α,TNF-α)和白細(xì)胞介素6(interleukin 6,IL-6)含量檢測(cè)。收集各組軟骨細(xì)胞的培養(yǎng)液,檢測(cè)上清液中TNF-α和IL-6含量。⑥軟骨細(xì)胞中circ_0045714表達(dá)量檢測(cè)。提取各組軟骨細(xì)胞中總RNA,逆轉(zhuǎn)錄合成cDNA,然后擴(kuò)增,最后計(jì)算circ_0045714的表達(dá)量。結(jié)果:①蒺藜皂苷濃度篩選結(jié)果。IL-1β組的軟骨細(xì)胞存活率低于正常組(LSD-t=15.396,P=0.000)。IL-1β組與IL-1β+25 μg·mL-1蒺藜皂苷組的軟骨細(xì)胞存活率比較,差異無統(tǒng)計(jì)學(xué)意義(LSD-t=1.555,P=0.918)。IL-1β+50 μg·mL-1蒺藜皂苷組、IL-1β+100 μg·mL-1蒺藜皂苷組、IL-1β+200 μg·mL-1蒺藜皂苷組、IL-1β+400 μg·mL-1蒺藜皂苷組的軟骨細(xì)胞存活率均高于IL-1β組(LSD-t=4.879,P=0.047; LSD-t=7.686,P=0.001; LSD-t=10.657,P=0.000; LSD-t=10.073,P=0.000)。IL-1β+400 μg·mL-1蒺藜皂苷組與IL-1β+200 μg·mL-1蒺藜皂苷組的軟骨細(xì)胞存活率比較,差異無統(tǒng)計(jì)學(xué)意義(LSD-t=0.584,P=0.999)。因此,選擇濃度為50 μg·mL-1、100 μg·mL-1、200 μg·mL-1的蒺藜皂苷進(jìn)行實(shí)驗(yàn),并分別標(biāo)記為蒺藜皂苷低劑量組、中劑量組、高劑量組。②軟骨細(xì)胞增殖抑制率、軟骨細(xì)胞凋亡率、軟骨細(xì)胞中TNF-α和IL-6含量檢測(cè)結(jié)果。IL-1β組的軟骨細(xì)胞增殖抑制率、軟骨細(xì)胞凋亡率、軟骨細(xì)胞中TNF-α和IL-6含量均高于對(duì)照組、IL-1β+蒺藜皂苷低劑量組、IL-1β+蒺藜皂苷中劑量組、IL-1β+蒺藜皂苷高劑量組(軟骨細(xì)胞增殖抑制率:LSD-t=55.829,P=0.000; LSD-t=8.879,P=0.001; LSD-t=20.507,P=0.000; LSD-t=30.315,P=0.000; 軟骨細(xì)胞凋亡率:LSD-t=27.508,P=0.000; LSD-t=5.076,P=0.032; LSD-t=11.689,P=0.000; LSD-t=21.284,P=0.000; 軟骨細(xì)胞中TNF-α含量:LSD-t=29.990,P=0.000; LSD-t=7.720,P=0.002; LSD-t=17.182,P=0.000; LSD-t=24.615,P=0.000; 軟骨細(xì)胞中IL-6含量:LSD-t=33.441,P=0.000; LSD-t=6.324,P=0.008; LSD-t=15.440,P=0.000; LSD-t=25.096,P=0.000)。IL-1β+蒺藜皂苷低劑量組的軟骨細(xì)胞增殖抑制率、軟骨細(xì)胞凋亡率、軟骨細(xì)胞中TNF-α和IL-6含量均高于IL-1β+蒺藜皂苷中劑量組、IL-1β+蒺藜皂苷高劑量組(軟骨細(xì)胞增殖抑制率:(LSD-t=11.627,P=0.000; LSD-t=21.436,P=0.000; 軟骨細(xì)胞凋亡率:LSD-t=6.613,P=0.006; LSD-t=16.209,P=0.000; 軟骨細(xì)胞中TNF-α含量:LSD-t=9.463,P=0.000; LSD-t=16.895,P=0.000; 軟骨細(xì)胞中IL-6含量:LSD-t=9.117,P=0.001; LSD-t=18.773,P=0.000)。IL-1β+蒺藜皂苷中劑量組的軟骨細(xì)胞增殖抑制率、軟骨細(xì)胞凋亡率、軟骨細(xì)胞中TNF-α和IL-6含量均高于IL-1β+蒺藜皂苷高劑量組(LSD-t=9.808,P=0.000; LSD-t=9.595,P=0.000; LSD-t=7.432,P=0.003; LSD-t=9.656,P=0.000)。③軟骨細(xì)胞中circ_0045714表達(dá)量檢測(cè)結(jié)果。IL-1β組軟骨細(xì)胞中circ_0045714表達(dá)量低于對(duì)照組、IL-1β+蒺藜皂苷低劑量組、IL-1β+蒺藜皂苷中劑量組、IL-1β+蒺藜皂苷高劑量組(LSD-t=43.218,P=0.000; LSD-t=9.487,P=0.000; LSD-t=22.136,P=0.000; LSD-t=34.785,P=0.000)。IL-1β+蒺藜皂苷低劑量組軟骨細(xì)胞中circ_0045714表達(dá)量低于IL-1β+蒺藜皂苷中劑量組、IL-1β+蒺藜皂苷高劑量組(LSD-t=12.649,P=0.000; LSD-t=25.298,P=0.000)。IL-1β+蒺藜皂苷中劑量組軟骨細(xì)胞中circ_0045714表達(dá)量低于IL-1β+蒺藜皂苷高劑量組(LSD-t=12.649,P=0.000)。④過表達(dá)和干擾circ_0045714的軟骨細(xì)胞構(gòu)建結(jié)果。轉(zhuǎn)染pcDNA、pcDNA-circ_0045714的軟骨細(xì)胞中circ_0045714表達(dá)量比較,差異有統(tǒng)計(jì)學(xué)意義(1.00±0.00,4.18±0.14,t=39.342,P=0.000),說明過表達(dá)circ_0045714的軟骨細(xì)胞構(gòu)建成功。轉(zhuǎn)染si-NC、si-circ_0045714的軟骨細(xì)胞中circ_0045714表達(dá)量比較,差異有統(tǒng)計(jì)學(xué)意義(1.00±0.00,0.28±0.04,t=31.177,P=0.000),說明干擾circ_0045714的軟骨細(xì)胞構(gòu)建成功。⑤過表達(dá)circ_0045714對(duì)IL-1β誘導(dǎo)的軟骨細(xì)胞影響結(jié)果。IL-1β+pcDNA-circ_0045714組的軟骨細(xì)胞增殖抑制率、軟骨細(xì)胞凋亡率、軟骨細(xì)胞中TNF-α和IL-6含量均低于IL-1β+pcDNA組(t=16.290,P=0.000; t=11.359,P=0.000; t=11.988,P=0.000; t=12.266,P=0.000)。⑥干擾circ_0045714對(duì)IL-1β誘導(dǎo)的軟骨細(xì)胞影響結(jié)果。IL-1β+蒺藜皂苷高劑量+si-circ_0045714組軟骨細(xì)胞增殖抑制率、軟骨細(xì)胞凋亡率、軟骨細(xì)胞中TNF-α和IL-6含量均高于IL-1β+蒺藜皂苷高劑量+si-NC組(t=9.586,P=0.001; t=9.120,P=0.001; t=7.069,P=0.002; t=10.548,P=0.001)。結(jié)論:蒺藜皂苷能抑制IL-1β誘導(dǎo)的軟骨細(xì)胞凋亡及炎癥因子表達(dá),具有治療骨關(guān)節(jié)炎的潛在價(jià)值,其作用機(jī)制可能與上調(diào)軟骨細(xì)胞中circ_0045714表達(dá)有關(guān),且200 μg·mL-1蒺藜皂苷的效果更佳。
Abstract:
Objective:To observe the effects of tribulus terrestris saponins(TTS)on interleukin-1β(IL-1β)-induced chondrocyte apoptosis and inflammatory cytokine secretion,and to explore its underlying mechanism.Methods:①The mouse chondrocytes(ATDC5 cells)were harvested and cultured in the Dulbecco's Modified Eagle's Medium(DMEM).After 24-hour culture,the ATDC5 cells were transfected with pcDNA-circ_0045714,pcDNA,si-circ_0045714,and si-NC,respectively,by using transfection reagents.②The ATDC5 cells were inoculated onto the 96-well plates,with one group cultured in normal DMEM(normal group),one group in DMEM containing 10 ng/mL IL-1β(IL-1β group),and the rest in DMEM adding with 10 ng/mL IL-1β and TTS with concentration of 25,50,100,200,and 400 μg/mL,respectively.After 24-hour culture,the survival rate of chondrocytes in each group was calculated,based on which the concentration of TTS used in subsequent experiments was determined.③The ATDC5 cells were divided into control group,IL-1β group,IL-1β+low-dose TTS(L-TTS)group,IL-1β+medium-dose TTS(M-TTS)group,and IL-1β+high-dose TTS(H-TTS)group.The ATDC5 cells in control group were cultured in conventional DMEM,the ones in IL-1β group in DMEM with 10 ng/mL IL-1β,and the ones in the other 3 groups in DMEM adding with 10 ng/mL IL-1β and TTS with concentration of 50,100,and 200 μg/mL,respectively.The pcDNA-circ_0045714- and pcDNA-transfected ATDC5 cells were cultured with the same method as IL-1β group,and were labeled as IL-1β+pcDNA-circ_0045714 group and IL-1β+pcDNA group,respectively.The si-circ_0045714- and si-NC-transfected ATDC5 cells were cultured with the same method as IL-1β+H-TTS group,and were labeled as IL-1β+H-TTS+si-circ_0045714 group and IL-1β+H-TTS+si-NC group,respectively.After 24-hour culture,the proliferation inhibition rate of the chondrocytes in each group was calculated.④The ATDC5 cells and the pcDNA-circ_0045714-,pcDNA-,si-circ_0045714- and si-NC-transfected ATDC5 cells were inoculated onto the 6-well plates.After 12-hour culture,the apoptosis rate of chondrocytes in each group was detected by using flow cytometry.⑤The culture medium of chondrocytes was collected from each group,and the levels of tumor necrosis factor-α(TNF-α)and interleukin 6(IL-6)in the supernatant were detected.⑥The total RNA was extracted from chondrocytes in each group for synthesizing the cDNA by reverse transcription,followed by amplification for calculating the expression level of circ_0045714 in chondrocytes.Results:①The survival rate of chondrocytes was lower in IL-1β group compared to normal group(LSD-t=15.396,P=0.000),and lower compared with that of IL-1β+50 μg/mL TTS group,IL-1β+100 μg/mL TTS group,IL-1β+200 μg/mL TTS group and IL-1β+400 μg/mL TTS group(LSD-t=4.879,P=0.047; LSD-t=7.686,P=0.001; LSD-t=10.657,P=0.000; LSD-t=10.073,P=0.000); while the differences between IL-1β group and IL-1β+25 μg/mL TTS group,and between IL-1β+200 μg/mL TTS group and IL-1β+400 μg/mL TTS group were not statistically significant(LSD-t=1.555,P=0.918; LSD-t=0.584,P=0.999).Therefore,the TTS with concentrations of 50,100 and 200 μg/mL were selected for the further experiments and were labeled as L-TTS group,M-TTS group,and H-TTS group,respectively,based on the concentrations.②The proliferation inhibition rate,the apoptosis rate,and the levels of TNF-α and IL-6 in the chondrocytes were higher in IL-1β group compared with those of control group,IL-1β+L-TTS group,IL-1β+M-TTS group,and IL-1β+H-TTS group(the proliferation inhibition rate:LSD-t=55.829,P=0.000; LSD-t=8.879,P=0.001; LSD-t=20.507,P=0.000; LSD-t=30.315,P=0.000; the apoptosis rate:LSD-t=27.508,P=0.000; LSD-t=5.076,P=0.032; LSD-t=11.689,P=0.000; LSD-t=21.284,P=0.000; the level of TNF-α in the chondrocytes:LSD-t=29.990,P=0.000; LSD-t=7.720,P=0.002; LSD-t=17.182,P=0.000; LSD-t=24.615,P=0.000; the level of IL-6 in the chondrocytes:LSD-t=33.441,P=0.000; LSD-t=6.324,P=0.008; LSD-t=15.440,P=0.000; LSD-t=25.096,P=0.000),and they were higher in IL-1β+L-TTS group compared to IL-1β+M-TTS group and IL-1β+H-TTS group(the proliferation inhibition rate:LSD-t=11.627,P=0.000; LSD-t=21.436,P=0.000; the apoptosis rate:LSD-t=6.613,P=0.006; LSD-t=16.209,P=0.000; the level of TNF-α in the chondrocytes:LSD-t=9.463,P=0.000; LSD-t=16.895,P=0.000; the level of IL-6 in the chondrocytes:LSD-t=9.117,P=0.001; LSD-t=18.773,P=0.000),furthermore,they were higher in IL-1β+M-TTS group compared to IL-1β+H-TTS group(LSD-t=9.808,P=0.000; LSD-t=9.595,P=0.000; LSD-t=7.432,P=0.003; LSD-t=9.656,P=0.000).③The expression level of circ_0045714 in the chondrocytes was lower in IL-1β group compared to control group,IL-1β+L-TTS group,IL-1β+M-TTS group,and IL-1β+H-TTS group(LSD-t=43.218,P=0.000; LSD-t=9.487,P=0.000; LSD-t=22.136,P=0.000; LSD-t=34.785,P=0.000),and was lower in IL-1β+L-TTS group compared to IL-1β+M-TTS group and IL-1β+H-TTS group(LSD-t=12.649,P=0.000; LSD-t=25.298,P=0.000),moreover,it was lowly expressed in IL-1β+M-TTS group compared to IL-1β+H-TTS group(LSD-t=12.649,P=0.000).④The expression level of circ_0045714 showed a significant difference in the chondrocytes transfected with pcDNA and pcDNA-circ_0045714(1.00±0.00,4.18±0.14,t=39.342,P=0.000),which indicated the chondrocytes with overexpression of circ_0045714 were successfully constructed.The difference in the expression level of circ_0045714 among the chondrocytes transfected with si-NC and si-circ_0045714 indicated the successful silencing of circ-0045714 in chondrocytes(1.00±0.00,0.28±0.04,t=31.177,P=0.000).⑤The IL-1β+pcDNA-circ_0045714 group showed lower proliferation inhibition rate,apoptosis rate,and TNF-α and IL-6 levels in the chondrocytes compared to IL-1β+pcDNA group(t=16.290,P=0.000; t=11.359,P=0.000; t=11.988,P=0.000; t=12.266,P=0.000).⑥The IL-1β+H-TTS+si-circ_0045714 group exhibited higher proliferation inhibition rate,apoptosis rate,and TNF-α and IL-6 levels in the chondrocytes compared to IL-1β+H-TTS+si-NC group(t=9.586,P=0.001; t=9.120,P=0.001; t=7.069,P=0.002; t=10.548,P=0.001).Conclusion:The TTS can inhibit IL-1β-induced chondrocyte apoptosis and the expression of inflammatory cytokine,which has potential value in treatment of osteoarthritis.It may exert the effects by upregulating the expression of circ_0045714 in the chondrocytes,and the effects are stronger at the concentration of 200 μg/mL.

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備注/Memo:
基金項(xiàng)目:保定市科技計(jì)劃項(xiàng)目(2041ZF019)
更新日期/Last Update: 1900-01-01