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[1]吳海洋.骨髓間充質(zhì)干細(xì)胞移植對(duì)膠原誘導(dǎo)性關(guān)節(jié)炎大鼠血清細(xì)胞因子的影響[J].中醫(yī)正骨,2019,31(08):14-19.
 WU Haiyang.Effects of bone marrow mesenchymal stem cells transplantation on serum cytokines in rats with collagen-induced arthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2019,31(08):14-19.
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骨髓間充質(zhì)干細(xì)胞移植對(duì)膠原誘導(dǎo)性關(guān)節(jié)炎大鼠血清細(xì)胞因子的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第31卷
期數(shù):
2019年08期
頁(yè)碼:
14-19
欄目:
基礎(chǔ)研究
出版日期:
2019-08-20

文章信息/Info

Title:
Effects of bone marrow mesenchymal stem cells transplantation on serum cytokines in rats with collagen-induced arthritis
作者:
吳海洋
(商丘市中心醫(yī)院,河南 商丘 476000)
Author(s):
WU Haiyang
Shangqiu Central Hospital, Shangqiu 476000,Henan,China
關(guān)鍵詞:
關(guān)節(jié)炎類風(fēng)濕 間質(zhì)干細(xì)胞移植 血管內(nèi)皮生長(zhǎng)因子類 基質(zhì)金屬蛋白酶3 腫瘤壞死因子α 白細(xì)胞介素17 動(dòng)物實(shí)驗(yàn) 大鼠
Keywords:
rthritisrheumatoid mesenchymal stem cell transplantation vascular endothelial growth factors matrix metalloproteinase 3 tumor necrosis factor-alpha interleukin-17 animal experimentation rats
摘要:
目的:觀察骨髓間充質(zhì)干細(xì)胞(bone marrow mesenchymal stem cells,BMSCs)移植對(duì)膠原誘導(dǎo)性關(guān)節(jié)炎(collagen induced arthritis,CIA)大鼠血清細(xì)胞因子的影響。方法:選取清潔級(jí)雌性Wistar大鼠60只。用頸椎脫臼斷頸法處死1只大鼠,取股骨和脛骨骨髓,分離、培養(yǎng)BMSCs。其余59只大鼠采用隨機(jī)數(shù)字表分為正常對(duì)照組(20只)、模型組(20只)、細(xì)胞移植組(19只)。模型組和細(xì)胞移植組每只大鼠在尾根部、背部及左后足跖部皮下注射含牛Ⅱ型膠原的乳化液共0.5 mL,正常對(duì)照組注射等量生理鹽水。于第1次注射后第14 天再注射1次。于第2次注射后的第15天,各組分別隨機(jī)處死2只大鼠,觀察左側(cè)踝關(guān)節(jié)組織形態(tài),判斷造模是否成功。于第2次注射的當(dāng)天,細(xì)胞移植組大鼠通過尾靜脈注射BMSCs懸液,正常對(duì)照組和模型組注射等量生理鹽水。
Abstract:
Objective:To observe the effects of bone marrow mesenchymal stem cells(BMSCs)transplantation on serum cytokines in rats with collagen-induced arthritis(CIA).Methods:Sixty clean-grade female Wistar rats were selected.One rat was randomly selected out from the 60 rats and was executed by using cervical dislocation method,and its bone marrow were fetched out from femur and tibia for separating and culturing BMSCs.The other 59 rats were randomly divided into normal control group(20),model group(20)and BMSCs transplantation group(19)by using random digits table.The rats in model group and BMSCs transplantation group were subcutaneously injected with emulsified liquid containing bovine typeⅡcollagen with dose of 0.5 mL into their tail roots,backs and left posterior plantar areas,while the rats in normal control group were subcutaneously injected with the same dose of normal saline(NS)in the same places.The second subcutaneous injection was performed on rats at the 14th day after the first injection.At 15th day after the second injection,2 rats were randomly selected out from each group and were executed,and their left ankle-joints were fetched out and the tissue morphology of left ankle-joints was observed to determine whether the models were built successfully.On the day of the second injection,the rats in BMSCs transplantation group were injected with BMSCs suspension into caudal vein,while the rats in normal control group and model group were injected with the same dose of NS into caudal vein.

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(收稿日期:2019-06-11 本文編輯:楊雅)
更新日期/Last Update: 2019-08-15