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[1]張文愷,涂冬鵬,許超,等.黃芪丹參超微顆粒凝膠對大鼠創(chuàng)面愈合影響的實(shí)驗研究[J].中醫(yī)正骨,2019,31(08):6-13.
　ZHANG Wenkai,TU Dongpeng,XU Chao,et al.Effects of ultrafine particle gel of astragalus membranaceus and salvia miltiorrhiza on wound healing in rats:an experimental study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2019,31(08):6-13.
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黃芪丹參超微顆粒凝膠對大鼠創(chuàng)面愈合影響的實(shí)驗研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第31卷
期數(shù):
2019年08期
頁碼:
6-13
欄目:
基礎(chǔ)研究
出版日期:
2019-08-20

文章信息/Info

Title:
Effects of ultrafine particle gel of astragalus membranaceus and salvia miltiorrhiza on wound healing in rats:an experimental study
作者:
張文愷1; 涂冬鵬1; 許超2; 王書卿1; 姜獻(xiàn)1; 周紅云1
(1.浙江中醫(yī)藥大學(xué)第二臨床醫(yī)學(xué)院,浙江 杭州 310053; 2.浙江中醫(yī)藥大學(xué)附屬第二醫(yī)院,浙江 杭州 310005)
Author(s):
ZHANG Wenkai1; TU Dongpeng1; XU Chao2; WANG Shuqing1; JIANG Xian1; ZHOU Hongyun1
1.The Second Clinical Medical College of Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China2.The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005,Zhejiang,China
關(guān)鍵詞:
傷口愈合;  黃芪;  丹參;  血管內(nèi)皮生長因子類;  毛細(xì)血管;  大鼠;  動物實(shí)驗
Keywords:
wound healing;  astragalus membranaceus;  salvia miltiorrhiza;  vascular endothelial growth factors;  capillaries;  rats;  animal experimentation
摘要:
目的:觀察黃芪丹參超微顆粒凝膠對大鼠創(chuàng)面愈合的影響,并探討其可能的作用機(jī)制。方法:將90只SD大鼠隨機(jī)分成模型組、敷貼組和凝膠組,每組30只。在所有大鼠背部制成直徑約1.8 cm的圓形全層皮膚切除開放創(chuàng)面。紗布止血,碘伏消毒后,模型組創(chuàng)面以無菌紗布包扎、敷貼組創(chuàng)面以愛力敷濕性敷貼包扎、凝膠組創(chuàng)面涂抹0.5 g黃芪丹參超微顆粒凝膠后用無菌紗布包扎,隔天換藥1次。分別于造模后第1天、第3天、第7天、第11天,從各組隨機(jī)選取6只大鼠,計算創(chuàng)面愈合率,并切取創(chuàng)面全層皮膚組織制成切片,計算創(chuàng)面修復(fù)組織中新生毛細(xì)血管數(shù)量和血管內(nèi)皮生長因子(vascular endothelial growth factor,VEGF)含量。造模后第15天,計算剩余6只大鼠創(chuàng)面愈合率,待創(chuàng)面完全愈合后處死。結(jié)果:①創(chuàng)面愈合率。造模后第1天,各組大鼠創(chuàng)面均無明顯變化,未計算創(chuàng)面愈合率。
Abstract:
Objective:To observe the effects of ultrafine particle gel of astragalus membranaceus and salvia miltiorrhiza on wound healing in rats and to explore its possible mechanism of actions.Methods:Ninety SD rats were randomly divided into model group,dressing group and gel group,30 cases in each group.A 1.8 mm diameter round open wound was made by resecting full-thickness skin of the back of all rats.After hemostasis with gauze and disinfection with iodophor,the wounds of rats in model group and dressing group were bandaged with sterile gauze and ALLEVYN THIN(愛力敷)moist dressing respectively and the wounds of rats in gel group were bandaged with sterile gauze after application of 0.5 g ultrafine particle gel of astragalus membranaceus and salvia miltiorrhiza.The fresh dressings were changed for the wounds on alternate days.Six rats were randomly selected out from each group at the 1st,3rd,7th and 11th day after the modeling respectively,and their wound healing rates were calculated.The full-thickness skin tissues of wound were sectioned,and the number of neogenetic blood capillary and the content of vascular endothelial growth factor(VEGF)in the repaired tissues of wound were calculated.The wound healing rates of the last six rats in each group were calculated at the 15th day after the modeling,and the rats were executed after the wound healed completely.Results:The wound changed unconspicuously in rats of each group at the 1st day after the modeling,so the wound healing rate was not calculated.

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備注/Memo

備注/Memo:
基金項目:浙江省中醫(yī)藥科技計劃項目(2018ZA048)通訊作者:許超 E-mail:[email protected](收稿日期:2019-04-29 本文編輯:李曉樂)
更新日期/Last Update: 2019-08-15