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[1]蔡立雄,霍志謙,孫丙銀,等.改良兔股骨骨缺損并骨延長(zhǎng)模型的建立[J].中醫(yī)正骨,2018,30(01):25-28.
　CAI Lixiong,HUO Zhiqian,SUN Bingyin,et al.Establishment of improved rabbit model of femoral defect and lengthening[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2018,30(01):25-28.
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改良兔股骨骨缺損并骨延長(zhǎng)模型的建立()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第30卷
期數(shù):
2018年01期
頁碼:
25-28
欄目:
基礎(chǔ)研究
出版日期:
2018-01-20

文章信息/Info

Title:
Establishment of improved rabbit model of femoral defect and lengthening
作者:
蔡立雄1; 霍志謙1; 孫丙銀2; 吳峰1; 溫建強(qiáng)1; 鄭揚(yáng)康1; 楊海韻1
1.廣東省佛山市中醫(yī)院,廣東 佛山 528000; 2.佛山健翔醫(yī)院,廣東 佛山 528000
Author(s):
CAI Lixiong1; HUO Zhiqian1; SUN Bingyin2; WU Feng1; WEN Jianqiang1; ZHENG Yangkang1; YANG Haiyun1
1.Foshan Hospital of Traditional Chinese Medicine,Foshan 528000,Guangdong,China 2.Foshan Jianxiang Hospital,Foshan 528000,Guangdong,China
關(guān)鍵詞:
骨膜;  骨延長(zhǎng)術(shù);  骨缺損;  兔;  模型; 動(dòng)物
Keywords:
Keywords periosteum;  bone lengthening;  bone defect;  rabbits;  models; animal
摘要:
目的:探討建立兔股骨骨缺損并骨延長(zhǎng)模型的有效方法。方法:將36只3～4月齡健康新西蘭白兔隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組。將自制骨延長(zhǎng)牽張器安裝于2組動(dòng)物左側(cè)股骨上后,于股骨中段截骨,做長(zhǎng)約12 mm的骨缺損,其中實(shí)驗(yàn)組截骨時(shí)保護(hù)股骨外側(cè)骨膜的連續(xù)性,對(duì)照組行全層截骨而未保護(hù)骨膜。靜息期1周后開始進(jìn)行骨延長(zhǎng)。于骨延長(zhǎng)結(jié)束后第28天,攝股骨X線片,觀察骨痂生長(zhǎng)情況,并取股骨標(biāo)本進(jìn)行肉眼大體觀察。結(jié)果:①股骨標(biāo)本X線檢查結(jié)果。實(shí)驗(yàn)組股骨干延長(zhǎng)區(qū)域呈高密度、均勻影像,新生骨質(zhì)區(qū)與兩斷端邊界模糊,外層皮質(zhì)連續(xù),可見大量新生骨痂生長(zhǎng); 對(duì)照組股骨干延長(zhǎng)區(qū)域呈較高密度、不均勻影像,新生骨質(zhì)區(qū)與股骨近端骨干連接部分邊界模糊,與股骨遠(yuǎn)端骨干連接部分邊界較清,尚存臺(tái)階,外層皮質(zhì)尚未完全連續(xù),可見新生骨痂生長(zhǎng)。②股骨標(biāo)本肉眼觀察結(jié)果。實(shí)驗(yàn)組骨延長(zhǎng)區(qū)域骨質(zhì)連續(xù),連接完整,骨痂量多,新生骨兩端界線模糊; 對(duì)照組骨延長(zhǎng)區(qū)域骨質(zhì)相對(duì)連續(xù),連接相對(duì)完整,見骨痂生長(zhǎng),新生骨遠(yuǎn)端尚有部分缺損,未完全填充骨缺損區(qū)。結(jié)論:在股骨截骨過程中保留部分骨膜,可以更有效地建立兔股骨骨缺損并骨延長(zhǎng)模型。
Abstract:
ABSTRACT Objective:To explore the effective method of building rabbit model of femoral defect and lengthening.Methods:Thirty-six 3-4-month-old healthy New Zealand white rabbits were randomly divided into experimental group and control group.The self-made bone lengthening-stretching device was fixed in the left femur of the rabbit,and osteotomy of middle femur was performed to make 12 mm long bone defect.The continuity of femoral lateral periosteum was protected when osteotomy was performed in experimental group,while the periosteum was not protected and full-thickness osteotomy was performed in control group.The bone lengthening surgery was carried out after one-week rest period.At the 28th day after the end of bone lengthening,the femoral X-ray films were taken,and the bony callus growth was observed,and then macroscopic observation was performed on the specimens of femurs.Results:The X-ray examination results of femur specimens demonstrated that in experimental group the bone lengthening area of femoral shaft presented with high density and homogeneous imaging,and the boundary between newly formed bone region and the two broken ends was fuzzy,meanwhile,the continuous outer cortex and a large number of newly formed bony calluses were found; while in control group the bone lengthening area of femoral shaft presented with high density and inhomogeneous imaging,and the boundary between newly formed bone region and the proximal femoral shaft was fuzzy,and the boundary between newly formed bone region and the distal femoral shaft was clear,meanwhile,the discontinuous outer cortex and some newly formed bony calluses were found.The results of macroscopic observation on femur specimens demonstrated that continuous bone tissues and a large number of bony calluses were found in the bone lengthening area of femoral shaft in experimental group,and the two ends of the newly formed bone were fuzzy; while partly continuous bone tissues and some bony calluses were found in the bone lengthening area of femoral shaft in control group,and partial bone defects could be found in the distal end of newly formed bone.Conclusion:The femoral defect and lengthening rabbit model can be built more effectively by preserving some periosteum in the process of the femoral osteotomy.

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備注/Memo

備注/Memo:
基金項(xiàng)目:廣東省佛山市科學(xué)技術(shù)局科研項(xiàng)目(2015AB00350) 通訊作者:楊海韻 E-mail:[email protected]
更新日期/Last Update: 2018-06-02