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[1]齊英娜,王延雷,吳鑫杰,等.改進(jìn)球囊導(dǎo)管法構(gòu)建大鼠急性壓迫型上頸脊髓損傷模型及模型大鼠傷后早期血液流變學(xué)指標(biāo)觀察[J].中醫(yī)正骨,2017,29(11):26-32.
　QI Yingna,WANG Yanlei,WU Xinjie,et al.Experimental research on acute-compression-type upper cervical spinal cord injury rat models created by using improved Foley's tube method and early observation on post-traumatic hemorheological indexes in rat models[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2017,29(11):26-32.
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改進(jìn)球囊導(dǎo)管法構(gòu)建大鼠急性壓迫型上頸脊髓損傷模型及模型大鼠傷后早期血液流變學(xué)指標(biāo)觀察()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第29卷
期數(shù):
2017年11期
頁(yè)碼:
26-32
欄目:
基礎(chǔ)研究
出版日期:
2017-11-20

文章信息/Info

Title:
Experimental research on acute-compression-type upper cervical spinal cord injury rat models created by using improved Foley's tube method and early observation on post-traumatic hemorheological indexes in rat models
作者:
齊英娜; 王延雷; 吳鑫杰; 王威; 郝慶英; 楊峰; 譚明生
中日友好醫(yī)院,北京 100029
Author(s):
QI Yingna; WANG Yanlei; WU Xinjie; WANG Wei; HAO Qingying; YANG Feng; TAN Mingsheng
China-Japan Friendship Hospital,Beijing 100029,China
關(guān)鍵詞:
脊髓損傷;  頸椎;  模型; 動(dòng)物;  大鼠; Wistar;  球囊導(dǎo)管;  血液流變學(xué)
Keywords:
Key words spinal cord injuries;  cervical vertebrae;  models; animal;  rats; Wistar;  Foley's tube;  hemorheology
摘要:
目的:探討改進(jìn)球囊導(dǎo)管法構(gòu)建大鼠急性壓迫型上頸脊髓損傷模型的有效性,并觀察模型大鼠傷后早期血液流變學(xué)指標(biāo)的變化。方法:將30只Wistar大鼠隨機(jī)分為空白組、假手術(shù)組和模型組,每組10只。空白組不進(jìn)行手術(shù)干預(yù)。假手術(shù)組和模型組大鼠經(jīng)寰枕間隙置入球囊導(dǎo)管; 操作時(shí)對(duì)譚明生教授等前期提出的方法進(jìn)行改進(jìn),以2 mL注射器針頭自制神經(jīng)剝離子,分離寰枕覆膜及寰樞椎間黃韌帶; 以縫合針和銅絲代替導(dǎo)絲,引導(dǎo)球囊導(dǎo)管經(jīng)寰枕間隙置入椎管內(nèi)。術(shù)后24 h以手推壓力泵向模型組大鼠球囊內(nèi)注射生理鹽水至球囊內(nèi)壓力達(dá)300 kPa,使球囊勻速膨脹,對(duì)頸脊髓產(chǎn)生壓迫。分別于球囊壓迫1 h、3 h、6 h、12 h、24 h后采用BBB評(píng)分法對(duì)各組大鼠的運(yùn)動(dòng)功能進(jìn)行評(píng)價(jià),最后一次運(yùn)動(dòng)功能評(píng)定結(jié)束后對(duì)各組大鼠進(jìn)行頸椎MRI檢查、血液流變學(xué)指標(biāo)測(cè)定及受壓節(jié)段脊髓組織病理學(xué)觀察。結(jié)果:①模型驗(yàn)證結(jié)果。實(shí)驗(yàn)期間共廢棄并補(bǔ)充4只大鼠。3組大鼠頸脊髓受到球囊壓迫后不同時(shí)間BBB評(píng)分結(jié)果顯示,時(shí)間因素和分組因素不存在交互效應(yīng)(F=0.310,P=0.736); 空白組和假手術(shù)組大鼠的BBB評(píng)分均高于模型組,即存在分組效應(yīng)(F=0.310,P=0.000); 球囊壓迫后不同時(shí)點(diǎn)之間BBB評(píng)分的差異無(wú)統(tǒng)計(jì)學(xué)意義,即不存在時(shí)間效應(yīng)(F=0.324,P=0.860); 3組大鼠的BBB評(píng)分隨時(shí)間推移均無(wú)明顯變化。MRI示各組大鼠頸椎序列均正常; 空白組大鼠頸部未見(jiàn)到球囊; 假手術(shù)組和模型組球囊壓迫系統(tǒng)穩(wěn)定、無(wú)松脫,球囊呈高信號(hào)改變,與脊髓等信號(hào)對(duì)比明顯; 模型組球囊膨脹好,向椎管內(nèi)單向均勻壓迫。受壓脊髓組織病理學(xué)觀察結(jié)果顯示,空白組和假手術(shù)組脊髓組織中未見(jiàn)或僅見(jiàn)極少數(shù)Fas陽(yáng)性凋亡細(xì)胞; Fas陽(yáng)性凋亡細(xì)胞在模型組脊髓組織中較多,廣泛分布于脊髓灰、白質(zhì)中。②模型大鼠傷后早期血液流變學(xué)指標(biāo)測(cè)定結(jié)果。脊髓受壓24 h后,除50/s外(F=0.602,P=0.663),3組大鼠血液在切變率為200/s、100/s、5/s、1/s時(shí)的全血黏度比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=2.713,P=0.042; F=6.358,P=0.000; F=18.893,P=0.000; F=7.320,P=0.000)。切變率為200/s時(shí),模型組的全血黏度高于空白組和假手術(shù)組(P=0.028,P=0.043),空白組與假手術(shù)組的全血黏度比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.851); 切變率為100/s時(shí),模型組的全血黏度高于空白組和假手術(shù)組(P=0.044,P=0.003),空白組與假手術(shù)組的全血黏度比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.972); 切變率為5/s時(shí),模型組的全血黏度高于空白組和假手術(shù)組(P=0.001,P=0.000),空白組與假手術(shù)組的全血黏度比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.865); 切變率為1/s時(shí),模型組的全血黏度高于空白組和假手術(shù)組(P=0.010,P=0.011),空白組與假手術(shù)組的全血黏度比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.972)。3組大鼠的血漿黏度比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(F=0.734,P=0.574)。3組大鼠紅細(xì)胞聚集指數(shù)比較,差異有統(tǒng)計(jì)學(xué)意義(F=5.335,P=0.001); 模型組的紅細(xì)胞聚集指數(shù)高于空白組和假手術(shù)組(P=0.003,P=0.007); 空白組與假手術(shù)組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.696)。3組大鼠紅細(xì)胞變形指數(shù)比較,差異有統(tǒng)計(jì)學(xué)意義(F=18.288,P=0.000); 模型組的紅細(xì)胞變形指數(shù)高于空白組和假手術(shù)組(P=0.000,P=0.000); 空白組與假手術(shù)組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.948)。結(jié)論:采用改進(jìn)球囊導(dǎo)管法構(gòu)建的大鼠上頸脊髓損傷模型,符合急性壓迫型上頸脊髓損傷的特點(diǎn); 模型大鼠的血液在受傷后24 h時(shí)呈高凝狀態(tài)。
Abstract:
ABSTRACT Objective:To explore the effectiveness of improved Foley's tube method in building acute-compression-type upper cervical spinal cord injury rat models,and to observe the early changes of hemorheological indexes after injury in rat models.Methods:Thirty Wistar rats were randomly divided into blank group,sham-operated group and model group,10 cases in each group.The rats in blank group were not given any surgical intervention,while the rats in the other two groups were intervened by inserting a Foley's tube into atlanto-occipital space.The method proposed by professor TAN Mingsheng.was improved in the operation.A 2 mL gauge syringe needle was made into a neural detacher to separate atlanto-occipital tectorial membrane and atlantoaxial intervertebral ligamentum flavum,and a Foley's tube was inserted into spinal canal through the atlanto-occipital space under the guidance of surgical sewing needle and copper wire instead of guide wire.At 24 hours after the surgery,the normal saline were injected into the Foley's tube in model group using manumotive force-pumps till the pressure reached 300 kPa.The balloon of Foley's tube was expanded in uniform speed to oppress the cervical spinal cord.The motor function of rats in each group were evaluated by using BBB scoring method after 1-,3-,6-,12- and 24-hour oppression with balloon respectively.Cervical MRI examination,determination of hemorheological indexes and histopathological observation on oppressed spinal cord were performed on rats in each group after the last motor function evaluation.Results:Four rats were abandoned during the experiment and the experiment were supplemented with another 4 rats.The BBB scores of the 3 groups at different time-points after the cervical spinal cord was compressed by balloon demonstrated that(1)there was no interaction between time factor and group factor(F=0.310,P=0.736);(2)the BBB scores were higher in blank group and sham-operated group compared to model group,in other words,there was group effect(F=0.310,P=0.000);(3)there was no statistical difference in BBB scores between different timepoints,in other words,there was no time effect(F=0.324,P=0.860);(4)the BBB scores changed unconspicuously with time in the 3 groups.The results of MRI examination demonstrated that(1)the cervical spine sequences of rats were normal in each group;(2)no balloon was found in the neck of rats of blank group;(3)the stable balloon compression system was found in rats of sham-operated group and model group and the balloon presented with high signal changes;(4)the balloon expanded well and unidirectional uniform compression of spinal canal was found in rats of model group.The results of histopathological observation on compressed spinal cord demonstrated that few Fas positive apoptotic cells were found in the spinal cord tissues of rats of blank group and sham-operated group,while many Fas positive apoptotic cells were found in the spinal cord tissues of rats of model group and they widely distributed in the spinal gray matter and white matter.After 24-hour spinal cord compression,there was statistical difference in the whole blood viscosity(WBV)between the 3 groups when the shear rate was 200/s,100/s,5/s and 1/s(F=2.713,P=0.042; F=6.358,P=0.000; F=18.893,P=0.000; F=7.320,P=0.000)and there was no statistical difference when the shear rate was 50/s(F=0.602,P=0.663).When the shear rate was 200/s,the WBV was higher in model group compared to blank group and sham-operated group(P=0.028,P=0.043),and there was no statistical difference in the WBV between blank group and sham-operated group(P=0.851).When the shear rate was 100/s,the WBV was higher in model group compared to blank group and sham-operated group(P=0.044,P=0.003),and there was no statistical difference in the WBV between blank group and sham-operated group(P=0.972).When the shear rate was 5/s,the WBV was higher in model group compared to blank group and sham-operated group(P=0.001,P=0.000),and there was no statistical difference in the WBV between blank group and sham-operated group(P=0.865).When the shear rate was 1/s,the WBV was higher in model group compared to blank group and sham-operated group(P=0.010,P=0.011),and there was no statistical difference in the WBV between blank group and sham-operated group(P=0.972).There was no statistical difference in plasma viscosity between the 3 groups(F=0.734,P=0.574).There was statistical difference in erythrocyte aggregation index between the 3 groups(F=5.335,P=0.001).The erythrocyte aggregation index was higher in model group compared to blank group and sham-operated group(P=0.003,P=0.007),and there was no statistical difference in erythrocyte aggregation index between blank group and sham-operated group(P=0.696).There was statistical difference in erythrocyte deformation index between the 3 groups(F=18.288,P=0.000).The erythrocyte deformation index was higher in model group compared to blank group and sham-operated group(P=0.000,P=0.000),and there was no statistical difference in erythrocyte deformation index between blank group and sham-operated group(P=0.948).Conclusion:The upper cervical spinal cord injury rat models created by using improved Foley's tube method fit the characteristics of acute-compression-type upper cervical spinal cord injury.The blood of rat models presents hypercoagulabale state at 24 hours after injury.

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備注/Memo

備注/Memo:
基金項(xiàng)目:首都臨床特色應(yīng)用研究專項(xiàng)課題(Z16110000516009) 通訊作者:譚明生 E-mail:[email protected]
更新日期/Last Update: 2018-04-02