84年鼠女哪年财运最旺,857comvvv色九欧美激情|85PO_87国产精品欲av国产av资源

[1]鄭文偉,翁霞萍,黃綏心,等.獨活寄生湯對骨關(guān)節(jié)炎軟骨退變的影響及其作用機制[J].中醫(yī)正骨,2017,29(07):5-11.
　ZHENG Wenwei,WENG Xiaping,HUANG Suixin,et al.Effects of Duhuo Jisheng Tang on cartilage degeneration in the process of osteoarthritis and the mechanism of action[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2017,29(07):5-11.
點擊復(fù)制

獨活寄生湯對骨關(guān)節(jié)炎軟骨退變的影響及其作用機制 ()
分享到：

《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第29卷
期數(shù):
2017年07期
頁碼:
5-11
欄目:
基礎(chǔ)研究
出版日期:
2017-07-20

文章信息/Info

Title:
Effects of Duhuo Jisheng Tang on cartilage degeneration in the process of osteoarthritis and the mechanism of action
作者:
鄭文偉1; 翁霞萍1; 黃綏心2; 鄭春松1; 葉錦霞1; 吳追樂2; 許惠鳳2; 吳明霞2; 李西海1; 劉獻祥1
1.福建中醫(yī)藥大學(xué),福建 福州 350122; 2.福建省中西醫(yī)結(jié)合老年性疾病重點實驗室,福建 福州 350122
Author(s):
ZHENG Wenwei1; WENG Xiaping1; HUANG Suixin2; ZHENG Chunsong1; YE Jinxia1; WU Zhuile2; XU Huifeng2; WU Mingxia2; LI Xihai1; LIU Xianxiang1
1.Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China 2.Fujian Key Laboratory of Integrated Medicine on Geriatrics,Fuzhou 350122,Fujian,China
關(guān)鍵詞:
骨關(guān)節(jié)炎;  獨活寄生湯;  軟骨退變;  G蛋白偶聯(lián)信號通路;  動物實驗
Keywords:
Key words osteoarthritis;  Duhuo Jisheng Tang;  cartilage degeneration;  G protein coupled signaling pathway;  animal experimentation
摘要:
目的:探討?yīng)毣罴纳鷾珜顷P(guān)節(jié)炎軟骨退變的影響及其作用機制。方法:將45只8周齡雄性SD大鼠隨機分為空白組、模型組、獨活寄生湯組,每組15只。模型組、獨活寄生湯組采用改良Hulth法建立膝骨關(guān)節(jié)炎大鼠模型,空白組不做任何處理。獨活寄生湯組給予9.3 g·kg-1的獨活寄生湯水煎濃縮液進行灌胃,模型組和空白組給予等劑量生理鹽水灌胃; 每天灌胃1次,連續(xù)灌胃12周。末次灌胃后分別取各組大鼠雙側(cè)股骨髁,采用HE染色觀察關(guān)節(jié)軟骨組織形態(tài),并采用Mankin's關(guān)節(jié)軟骨組織學(xué)評分法評價軟骨退變程度; 采用Western blot法檢測軟骨中G蛋白偶聯(lián)信號傳導(dǎo)系統(tǒng)的關(guān)鍵調(diào)控因子的蛋白表達情況。分別從模型組和獨活寄生湯組各取50 mg關(guān)節(jié)軟骨組織,采用基因芯片檢測軟骨基質(zhì)降解基因表達情況。結(jié)果:①軟骨組織形態(tài)。空白組關(guān)節(jié)軟骨結(jié)構(gòu)層次清晰,潮線完整,軟骨表面較為平滑; 模型組關(guān)節(jié)軟骨結(jié)構(gòu)層次較為紊亂,未見潮線,表層軟骨膜破損,膜樣結(jié)構(gòu)消失并呈絲絨樣; 獨活寄生湯組關(guān)節(jié)軟骨結(jié)構(gòu)層次較為清晰,軟骨破壞程度比模型組輕。②軟骨退變程度。3組大鼠關(guān)節(jié)軟骨的Mankin's組織學(xué)評分比較,差異有統(tǒng)計學(xué)意義[(3.46±2.04)分,(12.58±2.55)分,(7.75±1.91)分,F=13.114,P=0.006]; 空白組和獨活寄生湯組低于模型組(t=-5.114,P=0.002; t=2.709,P=0.035); 空白組與獨活寄生湯組比較,差異無統(tǒng)計學(xué)意義(t=-2.406,P=0.053)。③藥物干預(yù)后G蛋白偶聯(lián)信號傳導(dǎo)系統(tǒng)關(guān)鍵調(diào)控因子的蛋白表達。3組大鼠G蛋白偶聯(lián)信號傳導(dǎo)系統(tǒng)關(guān)鍵調(diào)控因子Gαs、Gαq、Gαo、Gαi蛋白表達量比較,組間差異均有統(tǒng)計學(xué)意義[(1.59±0.09)kDa,(1.01±0.04)kDa,(1.45±0.14)kDa,F=29.760,P=0.001;(1.03±0.06)kDa,(0.53±0.04)kDa,(0.75±0.09)kDa,F=14.969,P=0.027;(0.36±0.02)kDa,(0.11±0.01)kDa,(0.17±0.02)kDa,F=204.105,P=0.000;(0.20±0.02)kDa,(0.56±0.05)kDa,(0.33±0.07)kDa,F=28.357,P=0.001]; 空白組Gαs、Gαq、Gαo表達量均高于模型組(t=0.407,P=0.000; t=0.546,P=0.012; t=1.933,P=0.000),Gαi表達量低于模型組(t=-0.750,P=0.000); 模型組Gαs、Gαq、Gαo表達量均低于獨活寄生湯組(t=-5.584,P=0.001; t=-2.431,P=0.093; t=-4.584,P=0.004),Gαi表達量高于獨活寄生湯組(t=4.377,P=0.005); 獨活寄生湯組Gαs、Gαq表達量與空白組比較,組間差異均無統(tǒng)計學(xué)意義(t=-1.816,P=0.119; t=-3.029,P=0.056); 獨活寄生湯組Gαo表達量低于空白組(t=-14.749,P=0.000),Gαi表達量高于空白組(t=3.119,P=0.021)。④藥物干預(yù)后關(guān)節(jié)軟骨基質(zhì)降解基因的表達。藥物干預(yù)后,表達上調(diào)2倍以上的軟骨基質(zhì)降解基因為基質(zhì)金屬蛋白酶(matrix metalloproteinase,MMP)8、硒蛋白P(selenoprotein P,SELP),表達下調(diào)2倍以上的軟骨基質(zhì)降解基因為蛋白聚糖酶(a disintegrin and metalloproteinase with thrombospondin motifs,ADAMTS)1、ADAMTS2、ADAMTS8、鈣黏蛋白3(cadherin 3,CDH3)、膠原(collagen,COL)1A1、COL5A1、結(jié)締組織生長因子(connective tissue growth factor,CTGF)、整合素(integrin,ITG)b1、ITGb4、MMP12、MMP14。模型組MMP8、SELP基因表達量均低于獨活寄生湯組(1.00±0.00,2.81±0.60,t=-5.225,P=0.035; 1.00±0.00,2.31±0.81,t=-2.808,P=0.107),ADAMTS1、ADAMTS2、ADAMTS8、CDH3、COL1A1、COL5A1、CTGF、ITGb1、ITGb4、MMP12、MMP14基因表達量均高于獨活寄生湯組(1.00±0.00,0.21±0.05,t=27.366,P=0.001; 1.00±0.00,0.31±0.16,t=7.458,P=0.018; 1.00±0.00,0.11±0.04,t=38.538,P=0.001; 1.00±0.00,0.30±0.23,t=5.271,P=0.034; 1.00±0.00,0.12±0.09,t=17.380,P=0.003; 1.00±0.00,0.09±0.16,t=10.168,P=0.010; 1.00±0.00, 0.21±0.30,t=4.605,P=0.044; 1.00±0.00,0.37±0.20,t=5.456,P=0.032; 1.00±0.00,0.13±0.22,t=6.960,P=0.020; 1.00±0.00,0.05±0.08,t=19.388,P=0.003; 1.00±0.00,0.05±0.10,t=15.671,P=0.004)。結(jié)論:獨活寄生湯可以明顯延緩骨關(guān)節(jié)炎軟骨的退變,其作用機制可能是通過激活G蛋白偶聯(lián)信號傳導(dǎo)通路而抑制軟骨基質(zhì)降解,從而使受損的軟骨組織修復(fù),但其具體作用靶點有待進一步深入研究。
Abstract:
ABSTRACT Objective:To explore the effects of Duhuo Jisheng Tang(獨活寄生湯,DHJST)on cartilage degeneration in the process of osteoarthritis(OA)and the mechanism of action.Methods:Forty-five 8-week-old male SD rats were randomly divided into blank group,model group and DHJST group,15 cases in each group.The knee OA models were built by modified Hulth method in rats of model group and DHJST group,while the rats in blank group were not given any surgical intervention.The rats in DHJST group were intragastric administrated with DHJST concentrated solution in dosages of 9.3 g/kg,while the rats in model group and blank group were intragastric administrated with isodose normal saline,once a day for consecutive 12 weeks.The bilateral femoral condyles of rats in each group were fetched out respectively after the last intragastric administration.The articular cartilage tissue forms were observed after HE staining and the degrees of cartilage degeneration were evaluated by using Mankin's articular cartilage histological scores.The protein expression of key regulating factor of G protein coupled signal transduction systems in the cartilage were detected by using Western blot method.The articularological scores.The protein expression of key regulating factor of G protein coupled signal transduction systems in the cartilage were detected by using Western blot method.The articular cartilage(50 mg)were taken from the rats in model group and DHJST group respectively,and the cartilage matrix degradation gene expression were detected by using gene chips.Results:Clear articular cartilage structure,complete tidal line and smooth cartilage surface were found in blank group.Relatively disorderly articular cartilage structure and damaged surface perichondrium were found and tidal line and membrane structure disappeared in model group.Relatively clear articular cartilage structure and slighter cartilage destruction were found in DHJST group.There was statistical difference in Mankin's histological scores of articular cartilage between the 3 groups(3.46+/-2.04,12.58+/-2.55,7.75+/-1.91 points,F=13.114,P=0.006).The Mankin's histological scores were lower in blank group and DHJST group compared to model group(t=-5.114,P=0.002; t=2.709,P=0.035).There was no statistical difference in Mankin's histological scores between blank group and DHJST group(t=-2.406,P=0.053).There was statistical difference in the protein expression of key regulating factor Gαs,Gαq,Gαo and Gαi of G protein coupled signal transduction systems between the 3 groups(1.59+/-0.09,1.01+/-0.04,1.45+/-0.14 kDa,F=29.760,P=0.001; 1.03+/-0.06,0.53+/-0.04,0.75+/-0.09 kDa,F=14.969,P=0.027; 0.36+/-0.02,0.11+/-0.01,0.17+/-0.02 kDa,F=204.105,P=0.000; 0.20+/-0.02,0.56+/-0.05,0.33+/-0.07 kDa,F=28.357,P=0.001).The expressions of Gαs,Gαq and Gαo were higher and the expressions of Gαi were lower in blank group compared to model group(t=0.407,P=0.000; t=0.546,P=0.012; t=1.933,P=0.000; t=-0.750,P=0.000).The expressions of Gαs,Gαq and Gαo were lower and the expressions of Gαi were higher in model group compared to DHJST group(t=-5.584,P=0.001; t=-2.431,P=0.093; t=-4.584,P=0.004; t=4.377,P=0.005).There was no statistical difference in the expressions of Gαs and Gαq between blank group and DHJST group(t=-1.816,P=0.119; t=-3.029,P=0.056).The expressions of Gαo were lower and the expressions of Gαi were higher in DHJST group compared to blank group(t=-14.749,P=0.000; t=3.119,P=0.021).After drug intervention,the expression of some cartilage matrix degradation genes,including matrix metalloproteinase(MMP)8 and selenoprotein P(SELP),were up-regulated more than 2 times; while the expression of some cartilage matrix degradation genes,including a disintegrin and metalloproteinase with thrombospondin motifs(ADAMTS)1,ADAMTS2,ADAMTS8,cadherin 3(CDH3),collagen(COL)1A1,COL5A1,connective tissue growth factor(CTGF),integrin(ITG)b1,ITGb4,MMP12 and MMP14 were down-regulated more than 2 times.The gene expression levels of MMP8 and SELP were lower in model group compared to DHJST group(1.00+/-0.00 vs 2.81+/-0.60,t=-5.225,P=0.035; 1.00+/-0.00 vs 2.31+/-0.81,t=-2.808,P=0.107),while the gene expression levels of ADAMTS1,ADAMTS2,ADAMTS8,CDH3,COL1A1,COL5A1,CTGF,ITGb1,ITGb4,MMP12 and MMP14 were higher in model group compared to DHJST group(1.00+/-0.00 vs 0.21+/-0.05,t=27.366,P=0.001; 1.00+/-0.00 vs 0.31+/-0.16,t=7.458,P=0.018; 1.00+/-0.00 vs 0.11+/-0.04,t=38.538,P=0.001; 1.00+/-0.00 vs 0.30+/-0.23,t=5.271,P=0.034; 1.00+/-0.00 vs 0.12+/-0.09,t=17.380,P=0.003; 1.00+/-0.00 vs 0.09+/-0.16,t=10.168,P=0.010; 1.00+/-0.00 vs 0.21+/-0.30,t=4.605,P=0.044; 1.00+/-0.00 vs 0.37+/-0.20,t=5.456,P=0.032; 1.00+/-0.00 vs 0.13+/-0.22,t=6.960,P=0.020; 1.00+/-0.00 vs 0.05+/-0.08,t=19.388,P=0.003; 1.00+/-0.00 vs 0.05+/-0.10,t=15.671,P=0.004).Conclusion:DHJST can obviously delay cartilage degeneration in the process of OA.The mechanisms of action may be that it can inhibit cartilage matrix degradation through activating G protein coupled signaling pathway,so it can repair the damaged cartilage tissues.However,its specific action targets need to be further studied.

參考文獻/References:

[1] CORR EM,CUNNINGHAM CC,HELBERT L,et al.Osteoarthritis-associated basic calcium phosphate crystals activate membrane proximal kinases in human innate immune cells[J].Arthritis Res Ther,2017,19(1):23.
[2] MOUSSA M,LAJEUNESSE D,HILAL G,et al.Platelet rich plasma(PRP)induces chondroprotection via increasing autophagy,anti-inflammatory markers,and decreasing apoptosis in human osteoarthritic cartilage[J].Exp Cell Res,2017,352(1):146-156.
[3] 李西海,張翼,葉蕻芝,等.獨活寄生湯治療風(fēng)寒濕痹型膝骨關(guān)節(jié)炎的機制[J].中醫(yī)正骨,2012,24(1):68-71.
[4] 林木南,劉獻祥,王水良,等.治療型關(guān)節(jié)炎護膝對實驗性骨關(guān)節(jié)炎軟骨細胞凋亡基因Bcl-2及p53的影響[J].中國骨傷,2009,22(9):688-691.
[5] 鄭文偉,馬玉環(huán),陳后煌,等.跳骨片抑制大鼠膝骨關(guān)節(jié)炎Wnt/β-catenin信號轉(zhuǎn)導(dǎo)通路介導(dǎo)炎癥反應(yīng)的機制研究[J].國際骨科學(xué)雜志,2017,38(1):60-64.
[6] 林木南,劉獻祥,李西海,等.治療型關(guān)節(jié)炎護膝對膝骨關(guān)節(jié)炎兔關(guān)節(jié)液中轉(zhuǎn)化生長因子β及胰島素樣生長因子的影響[J].中醫(yī)正骨,2012,24(1):11-14.
[7] 黃繼漢,黃曉暉,陳志揚,等.藥理試驗中動物間和動物與人體間的等效劑量換算[J].中國臨床藥理學(xué)與治療學(xué),2004,9(9):1069-1072. 中醫(yī)正骨2017年7月第29卷第7期 J Trad Chin Orthop Trauma,2017,Vol.29,No.7(總491) (總492)中醫(yī)正骨2017年7月第29卷第7期 J Trad Chin Orthop Trauma,2017,Vol.29,No.7
[8] MANKIN HJ,JOHNSON ME,LIPPIELLO L.Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips.III. Distribution and metabolism of amino sugar-containing macromolecules[J].J Bone Joint Surg Am,1981,63(1):131-139.
[9] 李西海,陳文列,劉獻祥.補腎柔肝法防治骨性關(guān)節(jié)炎作用機制探討[J].福建中醫(yī)藥大學(xué)學(xué)報,2011,21(2):66-69.
[10] 周肅陵,劉肖瑜,王正.獨活寄生湯在骨傷科的臨床應(yīng)用[J].中醫(yī)正骨,2006,18(12):67-69.
[11] 盧躍卿,任小巧,邢海燕.中醫(yī)肝腎同源與神經(jīng)內(nèi)分泌免疫網(wǎng)絡(luò)關(guān)系[J].中華實用中西醫(yī)雜志,2000,13(4):765.
[12] 王耀光.“肝腎同源”論初探[J].中醫(yī)雜志,2008,49(1):5-7.
[13] STEFFENS B,SAUTER M.G proteins as regulators in ethylene-mediated hypoxia signaling[J].Plant Signal Behav,2010,5(4):375-378.
[14] TESMER JJ.The quest to understand heterotrimeric G protein signaling[J].Nat Struct Mol Biol,2010,17(6):650-652.
[15] BASCOUL-COLOMBO C,GARAIOVA I,PLUMMER SF,et al.Glucosamine hydrochloride but not chondroitin sulfate prevents cartilage degradation and inflammation induced by interleukin-1α in bovine cartilage explants[J].Cartilage,2016,7(1):70-81.
[16] 邵翔,陳后煌,陳達,等.透骨消痛膠囊抑制脂多糖誘導(dǎo)軟骨細胞炎癥反應(yīng)的機制研究[J].國際骨科學(xué)雜志,2016,37(6):406-410.
[17] WANG CC,GUO L,TIAN FD,et al.Naringenin regulates production of matrix metalloproteinases in the knee-joint and primary cultured articular chondrocytes and alleviates pain in rat osteoarthritis model[J].Brazilian Journal of Medical and Biological Research,2017,50(4):e5714.
[18] ZHANG Y,LIN J,WEI F.The function and roles of ADAMTS-7 in inflammatory diseases[J].Mediators Inflamm,2015,(9):1-11.
[19] MONFOULET LE,PHILIPPE C,MERCIER S,et al.Deficiency of G-protein coupled receptor 40,a lipid-activated receptor,heightens in vitro- and in vivo-induced murine osteoarthritis[J].Exp Biol Med(Maywood),2015,240(7):854-866.
[20] VARANI K,VINCENZI F,TOSI A,et al.Expression and functional role of adenosine receptors in regulating inflammatory responses in human synoviocytes[J].Br J Pharmacol,2010,160(1):101-115.
[21] BERG V,SVEINBJÖRNSSON B,BENDIKSEN S,et al.Human articular chondrocytes express ChemR23 and chemerin; ChemR23 promotes inflammatory signalling upon binding the ligand chemerin 21-157[J].Arthritis Res Ther,2010,12(6):R228.
[22] 劉俊才,陳友霞,李忠,等.整合素α5β1在膝骨關(guān)節(jié)炎不同退變程度關(guān)節(jié)軟骨中的差異性表達[J].中國組織工程研究,2017,21(8):1155-1160.

相似文獻/References:

[1]孟維娜,明立功,王新德,等.關(guān)節(jié)鏡下清理聯(lián)合腓骨近1/3段截骨治療膝骨關(guān)節(jié)炎[J].中醫(yī)正骨,2015,27(11):40.
[2]明立功,孟維娜,王新德,等.腓骨近端截骨治療內(nèi)側(cè)間室膝骨關(guān)節(jié)炎的近期療效觀察[J].中醫(yī)正骨,2015,27(10):25.
[3]張杰,王人彥,張玉柱.膝骨關(guān)節(jié)炎的治療進展[J].中醫(yī)正骨,2015,27(10):68.
[4]梁朝,蔡靜怡,閆立,等.針刀療法改善膝骨關(guān)節(jié)炎早期疼痛癥狀的療效評價[J].中醫(yī)正骨,2015,27(09):9.
　LIANG Zhao,CAI Jingyi,YAN Li,et al.Evaluation of the curative effect of needle-knife therapy for relieving knee pain in patients with early knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(07):9.
[5]王建武,黨建軍,李強,等.四聯(lián)療法治療膝骨關(guān)節(jié)炎[J].中醫(yī)正骨,2015,27(08):44.
[6]劉紅娟,郭會利,郭樹農(nóng).云克聯(lián)合中藥治療膝骨關(guān)節(jié)炎的護理[J].中醫(yī)正骨,2015,27(08):75.
[7]陳衛(wèi)衡.探索建立系統(tǒng)的膝骨關(guān)節(jié)炎中醫(yī)臨床科研范式 和理論體系[J].中醫(yī)正骨,2015,27(07):1.
[8]鄭春松,葉蕻芝,李西海,等.透骨消痛膠囊中補腎柔肝藥和活血祛風(fēng)藥治療 骨關(guān)節(jié)炎作用方式的計算機模擬比較[J].中醫(yī)正骨,2015,27(07):6.
　ZHENG Chunsong,YE Hongzhi,LI Xihai,et al.Comparison of the mode of action of Bushen Rougan(補腎柔肝)drugs versus Huoxue Qufeng(活血祛風(fēng))drugs contained in Tougu Xiaotong Jiaonang(透骨消痛膠囊)for the treatment of osteoarthritis:A computer simulation study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(07):6.
[9]帥波,沈霖,楊艷萍,等.加味青娥丸治療膝骨關(guān)節(jié)炎的作用機制研究[J].中醫(yī)正骨,2015,27(07):15.
　SHUAI Bo,SHEN Lin,YANG Yanping,et al.Study on the mechanism of action of Jiawei Qing'e Wan(加味青娥丸)for the treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(07):15.
[10]梅其杰,袁長深,段戡,等.壯藥骨痹方燙熨聯(lián)合運動療法治療膝骨關(guān)節(jié)炎的臨床研究[J].中醫(yī)正骨,2015,27(07):27.
　MEI Qijie,YUAN Changshen,DUAN Kan,et al.Clinical study of the curative effect of hot compressing and rubbing with packet of Gubi Fang(骨痹方)combined with exercise therapy in the treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(07):27.
[11]鄭春松,葉蕻芝,李西海,等.獨活寄生湯含藥血清對白細胞介素1β誘導(dǎo)的 退變關(guān)節(jié)軟骨細胞中基質(zhì)金屬蛋白酶 和環(huán)氧化酶2表達的影響[J].中醫(yī)正骨,2015,27(12):1.
　ZHENG Chunsong,YE Hongzhi,LI Xihai,et al.Impact of Duhuo Jisheng Tang(獨活寄生湯)medicated serum on expression of matrix metalloproteinase and cyclooxygenase 2 in degenerative articular chondrocytes induced by interleukin-1 beta[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(07):1.
[12]何曉娟,林平冬,馬玉環(huán),等.獨活寄生湯含藥血清抑制白細胞介素1β誘導(dǎo)的軟骨細胞炎癥反應(yīng)的作用機制研究[J].中醫(yī)正骨,2017,29(08):1.
　HE Xiaojuan,LIN Pingdong,MA Yuhuan,et al.Study on mechanism of action of Duhuo Jisheng Tang(獨活寄生湯)medicated serum in inhibiting inflammatory reaction induced by interleukin-1 beta in chondrocytes[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2017,29(07):1.
[13]陳俊,吳廣文,許惠鳳,等.獨活寄生湯含藥血清對大鼠退變軟骨細胞蛋白激酶 R樣內(nèi)質(zhì)網(wǎng)激酶/免疫球蛋白結(jié)合蛋白信號通路的影響[J].中醫(yī)正骨,2018,30(08):1.
　CHEN Jun,WU Guangwen,XU Huifeng,et al.Effect of Duhuo Jisheng Tang(獨活寄生湯)medicated serum on protein kinase R-like endoplasmic reticulum kinase/immunoglobulin-binding protein signaling pathway in degenerated chondrocytes of rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2018,30(07):1.
[14]朱勇,李藝彬,王世捷,等.關(guān)節(jié)鏡清理術(shù)配合口服獨活寄生湯 治療膝骨關(guān)節(jié)炎肝腎虧虛證[J].中醫(yī)正骨,2018,30(09):73.
[15]周江濤,趙依娜,王慶來.獨活寄生湯口服聯(lián)合玻璃酸鈉關(guān)節(jié)腔注射治療早中期膝骨關(guān)節(jié)炎[J].中醫(yī)正骨,2019,31(02):18.
　ZHOU Jiangtao,ZHAO Yina,WANG Qinglai.Oral application of Duhuo Jisheng Tang(獨活寄生湯)combined with intra-articular injection of sodium hyaluronate for treatment of early-middle knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2019,31(07):18.
[16]鄭梓灌,林濤,吳麗芳.獨活寄生湯口服聯(lián)合二烏紅花飲離子導(dǎo)入治療膝骨關(guān)節(jié)炎腎虛髓虧證[J].中醫(yī)正骨,2019,31(05):38.
[17]許麗梅,李慧,許云騰,等.基于NF-κB信號通路探討?yīng)毣罴纳鷾种浦嗵钦T導(dǎo)的軟骨細胞炎癥反應(yīng)的作用機制[J].中醫(yī)正骨,2019,31(07):9.
　XU Limei,LI Hui,XU Yunteng,et al.An experimental study of mechanism of action of Duhuo Jisheng Tang(獨活寄生湯)in inhibiting inflammatory reaction induced by lipopolysaccharide in chondrocytes based on NF-κB signaling pathway[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2019,31(07):9.
[18]黃巖石.獨活寄生湯口服聯(lián)合富血小板血漿關(guān)節(jié)腔內(nèi)注射治療膝骨關(guān)節(jié)炎的臨床研究[J].中醫(yī)正骨,2021,33(02):1.
　HUANG Yanshi.A clinical study of oral application of Duhuo Jisheng Tang(獨活寄生湯)combined with intra-articular injection of platelet-rich plasma for treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2021,33(07):1.
[19]韓玫,李貞,曹建西.溫針灸聯(lián)合口服獨活寄生湯治療膝骨關(guān)節(jié)炎寒濕痹阻證[J].中醫(yī)正骨,2021,33(06):67.
[20]陳俊,鄭若曦,葉錦霞,等.獨活寄生湯治療大鼠膝骨關(guān)節(jié)炎的作用機制研究[J].中醫(yī)正骨,2021,33(11):6.
　CHEN Jun,ZHENG Ruoxi,YE Jinxia,et al.Study on mechanism of action of Duhuo Jisheng Tang(獨活寄生湯)in treatment of knee osteoarthritis in rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2021,33(07):6.

備注/Memo

備注/Memo:
基金項目:國家自然科學(xué)基金項目(81373818) 通訊作者:李西海 E-mail:[email protected] mechanism of action
更新日期/Last Update: 2017-12-29