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[1]譚明生,齊英娜,姜良海,等.球囊導(dǎo)管構(gòu)建大鼠督脈瘀阻型上頸脊髓損傷模型的研究[J].中醫(yī)正骨,2016,28(12):1-5.
　TAN Mingsheng,QI Yingna,JIANG Lianghai,et al.A governor-vessel-blockade-type upper cervical spinal cord injury rat model created using Foley’s tube[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2016,28(12):1-5.
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球囊導(dǎo)管構(gòu)建大鼠督脈瘀阻型上頸脊髓損傷模型的研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第28卷
期數(shù):
2016年12期
頁碼:
1-5
欄目:
基礎(chǔ)研究
出版日期:
2016-12-30

文章信息/Info

Title:
A governor-vessel-blockade-type upper cervical spinal cord injury rat model created using Foley’s tube
作者:
譚明生1; 齊英娜2; 姜良海1; 劉楚吟2; 王延雷2; 王威2; 郝慶英1; 移平1; 楊峰1; 唐向盛1
1.中日友好醫(yī)院,北京 100029;
2.北京中醫(yī)藥大學(xué),北京 100029
Author(s):
TAN Mingsheng1; QI Yingna2; JIANG Lianghai1; LIU Chuyin2; WANG Yanlei2; WANG Wei2; HAO Qingying1; YI Ping1; YANG Feng1; TANG Xiangsheng1
1.China-Japan Friendship Hospital,Beijiang 100029,China 2. Beijing University of Chinese Medicine,Beijing 100029,China
關(guān)鍵詞:
脊髓損傷;  督脈瘀阻;  頸椎;  模型; 動物;  大鼠; Wistar;  球囊導(dǎo)管
Keywords:
spinal cord injuries;  blockade of governor vessel;  cervical vertebrae;  models; animal;  rats; Wistar;  Foley's tube
摘要:
目的:探討構(gòu)建大鼠督脈瘀阻型上頸脊髓損傷模型的有效方法。方法:選取30只3月齡SPF級Wistar大鼠,隨機(jī)分為正常組、假手術(shù)組和模型組,每組10只。正常組不進(jìn)行處理,其余2組大鼠經(jīng)寰枕間隙置入球囊導(dǎo)管。麻醉蘇醒后24 h,正常組和假手術(shù)組大鼠直接進(jìn)行實驗觀察; 在模型組大鼠的球囊導(dǎo)管末端連接手推壓力泵,推注碘海醇注射液加壓至3 bar(1 bar=100 kPa),持續(xù)加壓1 h后進(jìn)行實驗觀察。觀察各組大鼠活動情況及體征,采集四診信息,按照自擬的督脈瘀阻證辨證標(biāo)準(zhǔn)進(jìn)行中醫(yī)辨證。對各組大鼠行頸椎MRI檢查,選擇橫斷位T2WI上脊髓受壓最嚴(yán)重的層面,測定脊髓最小徑和最大徑,計算脊髓壓迫率(最小徑∕最大徑)。同時采用改良Tarlov評分法和BBB評分法對假手術(shù)組和模型組大鼠的運動功能進(jìn)行評定。結(jié)果:造模后正常組和假手術(shù)組大鼠精神狀態(tài)良好,喜動,活動靈活,皮毛光澤,唇色淡紅,耳色淡,爪色淡紅; 模型組大鼠精神萎靡,蜷縮,活動減少,步態(tài)不穩(wěn),垂尾,唇色暗紅,耳暗紅,爪色青紫。按照自擬的辨證標(biāo)準(zhǔn),模型組屬督脈瘀阻證。模型組脊髓壓迫率低于假手術(shù)組(0.64±0.04,0.73±0.04,t=4.672,P=0.000)。3組大鼠Tarlov評分、BBB評分總體比較,組間差異均有統(tǒng)計學(xué)意義[(4.98±0.18)分,(4.82±0.43)分,(4.17±0.60)分,F=9.408,P=0.001;(20.91±0.23)分,(21.01±0.36)分,(16.10±0.71)分,F=111.516,P=0.000]; 正常組和假手術(shù)組Tarlov評分、BBB評分比較,組間差異均無統(tǒng)計學(xué)意義(P=0.420; P=0.631); 模型組Tarlov評分、BBB評分均低于正常組和假手術(shù)組(P=0.000,P=0.000; P=0.003,P=0.000)。結(jié)論:經(jīng)寰枕間隙置入球囊導(dǎo)管并注射造影劑加壓,可構(gòu)建大鼠督脈瘀阻型上頸脊髓損傷模型。
Abstract:
Objective:To explore the effective method for building a governor-vessel-blockade-type upper cervical spinal cord injury rat model.Methods:Thirty 3-month-old SPF-grade Wistar rats were selected and randomly divided into normal group,sham-operated group and model group,10 cases in each group.The rats in normal group did not receive any treatment,while the rats in the other two groups were treated by inserting a Foley's tube into atlanto-occipital space.At 24 hours after anesthesia recovery,the rats in normal group and sham-operated group were observed immediately; while in model group the manumotive force-pumps were connected with the tail end of Foley's tube and the iohexol injections were injected into the Foley's tube continuously till the pressure reached 3 bar(1 bar=100 kPa).One hour later,the experimental observation was performed on the rats of model group.The activities and signs of all the rats were observed and their diagnostic informations were collected and the traditional Chinese medicine(TCM)syndrome differentiation were carried out according to self-made syndrome differentiation standard.The cervical MRI examination were performed on rats in each group and the transverse T2-weighted MRI on which the most serious compression of spinal cord were found were selected for measuring the minimal and maximal diameters of spinal cord,and then the spinal cord compression ratios(ratio of minimal diameter to maximal diameter of spinal cord)were calculated.The motor function were evaluated by using improved Tarlov scoring method and BBB scoring method in rats of sham-operated group and model group.Results:After modeling,the rats in normal group and sham-operated group were in good mental states and liked activities and presented with flexible movement,shiny fur,reddish lips,light-colored ears and reddish paws.The rats in model group were in bad mental states and dislike activities and presented with instability of gait,drooping tail,dull-red lips and ears and livid paws. According to the self-made syndrome differentiation standard,the TCM symptom complex of rats in model group categorized as governor-vessel-blockade syndrome.The spinal cord compression ratio was lower in model group compared to sham-operated group(0.64+/-0.04 vs 0.73+/-0.04,t=4.672,P=0.000).There was statistical difference in Tarlov scores and BBB scores between the 3 groups(4.98+/-0.18,4.82+/-0.43,4.17+/-0.60 points,F=9.408,P=0.001; 20.91+/-0.23,21.01+/-0.36,16.10+/-0.71 points,F=111.516,P=0.000).There was no statistical difference in Tarlov scores and BBB scores between normal group and sham-operated group(P=0.420; P=0.631).The Tarlov scores and BBB scores were lower in model group compared to normal group and sham-operated group(P=0.000,P=0.000; P=0.003,P=0.000).Conclusion:The governor-vessel-blockade-type upper cervical spinal cord injury rat models can be build by inserting Foley's tube and injecting contrast medium under pressuse into the atlanto-occipital space of rats.

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備注/Memo

備注/Memo:
基金項目:國家自然科學(xué)基金項目(81173423)
通訊作者:譚明生 E-mail:[email protected]
更新日期/Last Update: 2016-12-30