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[1]馮建軍,鄒浩,朱海燕,等.槲皮素對(duì)去卵巢大鼠血清骨鈣素和股骨Ⅰ型膠原蛋白水平的影響[J].中醫(yī)正骨,2016,28(07):10-13.
　FENG Jianjun,ZOU Hao,ZHU Haiyan,et al.Leverage bone grafting and pedicle screw internal fixation through posterior approach for treatment of thoracolumbar fractures[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2016,28(07):10-13.
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槲皮素對(duì)去卵巢大鼠血清骨鈣素和股骨Ⅰ型膠原蛋白水平的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第28卷
期數(shù):
2016年07期
頁(yè)碼:
10-13
欄目:
基礎(chǔ)研究
出版日期:
2016-07-20

文章信息/Info

Title:
Leverage bone grafting and pedicle screw internal fixation through posterior approach for treatment of thoracolumbar fractures
作者:
馮建軍; 鄒浩; 朱海燕; 甘一波
湖北省襄陽(yáng)市東風(fēng)人民醫(yī)院,湖北 襄陽(yáng) 441004
Author(s):
FENG Jianjun; ZOU Hao; ZHU Haiyan; GAN Yibo
The Dongfeng People's Hospital,Xiangyang 441004,Hubei,China
關(guān)鍵詞:
槲皮素;  骨質(zhì)疏松; 絕經(jīng)后;  骨鈣素;  膠原Ⅰ型;  動(dòng)物實(shí)驗(yàn);  大鼠
Keywords:
quercetin;  osteoporosis; postmenopausal;  osteocalcin;  collagen typeⅠ;  animal experimentation;  rats
摘要:
目的:觀察槲皮素對(duì)去卵巢大鼠血清骨鈣素和股骨Ⅰ型膠原蛋白水平的影響。方法:將50只6月齡清潔級(jí)健康雌性SD大鼠隨機(jī)分為假手術(shù)組、模型組、尼爾雌醇組、槲皮素高劑量組和槲皮素低劑量組,每組10只。假手術(shù)組大鼠開腹找到卵巢后即關(guān)閉腹腔,其余4組切除雙側(cè)卵巢。造模手術(shù)1周后,假手術(shù)組和模型組以生理鹽水灌胃,每天1次,每次4 mL; 尼爾雌醇組按1.5 mg·kg-1以尼爾雌醇灌胃,每周1次; 槲皮素高劑量組和槲皮素低劑量組分別按200 mg·kg-1和100 mg·kg-1以槲皮素灌胃,每天1次。3個(gè)月后以酶聯(lián)免疫吸附測(cè)定法測(cè)定各組大鼠血清骨鈣素水平,以Western Blot法測(cè)定股骨干骺端Ⅰ型膠原蛋白水平。結(jié)果:至藥物干預(yù)結(jié)束時(shí),模型組、尼爾雌醇組及槲皮素高劑量組各有1只大鼠死亡,槲皮素低劑量組2只大鼠死亡,死亡原因均為肺部感染。5組大鼠血清骨鈣素水平比較,差異有統(tǒng)計(jì)學(xué)意義[(2.562±0.671)ng·mL-1,(5.763±0.612)ng·mL-1,(2.674±1.170)ng·mL-1,(2.722±1.063)ng·mL-1,(3.574±1.914)ng·mL-1,F=98.527,P=0.000]。假手術(shù)組、尼爾雌醇組、槲皮素高劑量組、槲皮素低劑量組血清骨鈣素水平均低于模型組(P=0.000,P=0.000,P=0.000,P=0.005); 槲皮素高劑量組與尼爾雌醇組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.361); 槲皮素低劑量組高于尼爾雌醇組(P=0.047); 槲皮素低劑量組與槲皮素高劑組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.106)。5組大鼠股骨干骺端Ⅰ型膠原蛋白表達(dá)水平比較,差異有統(tǒng)計(jì)學(xué)意義(2.718±0.362,1.492±0.228,2.624±0.296,2.497±0.274,1.713±0.327,F=10.726,P=0.000)。假手術(shù)組、尼爾雌醇組、槲皮素高劑量組股骨Ⅰ型膠原蛋白表達(dá)水平均高于模型組(P=0.000,P=0.000,P=0.000); 槲皮素低劑量組與模型組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.056); 槲皮素高劑量組與尼爾雌醇組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.217); 槲皮素低劑量組低于槲皮素高劑量組和尼爾雌醇組(P=0.000,P=0.000)。結(jié)論:槲皮素具有類雌激素樣作用,可有效降低去卵巢大鼠的血清骨鈣素水平、提高股骨Ⅰ型膠原蛋白水平,其作用效果與劑量有關(guān)。
Abstract:
Objective:To observe the effect of quercetin on serum osteocalcin levels and femur typeⅠcollagen protein levels in the ovariectomized rats.Methods:Fifty healthy 6-month-old clean-grade female SD rats were randomly divided into sham-operated group,model group,nylestriol group,quercetin high-dose group and quercetin low-dose group,10 cases in each group.The rats in sham-operated group were treated with sham-operation,while the rats in other 4 groups were treated with bilateral ovariectomy.One week after the modeling,the rats in sham-operated group and model group were intragastric administrated with normal saline,once a day,4 ml at a time; and the rats in nylestriol group were intragastric administrated with nilestriol(1.5 mg/kg),once a week; and the rats in quercetin high-dose group and quercetin low-dose group were intragastric administrated with quercetin(200 and 100 mg/kg,respectively),once a day.Three months later,the serum osteocalcin levels were measured by using enzyme linked immunosorbent assays and the typeⅠcollagen protein levels of femoral metaphysis were measured by using Western Blot assays.Results:Some rats died from pulmonary infection in model group(1),nilestriol(1),quercetin high-dose group(1)and quercetin low-dose group(2)at the end of drug intervention.There was statistical difference in serum osteocalcin level between the 5 groups(2.562+/-0.671,5.763+/-0.612,2.674+/-1.170,2.722+/-1.063,3.574+/-1.914 ng/ml,F=98.527,P=0.000).The serum osteocalcin levels were lower in sham-operated group,nilestriol group,quercetinhigh-dose group and quercetin low-dose group compared to model group(P=0.000,P=0.000,P=0.000,P=0.005).There was no statistical difference in the serum osteocalcin levels between quercetin high-dose group and nilestriol group(P=0.361).The serum osteocalcin level were higher in quercetin low-dose group compared to nilestriol group(P=0.047).There was no statistical difference in serum osteocalcin levels between quercetin low-dose group and quercetin high-dose group(P=0.106).There was statistical difference in typeⅠcollagen protein levels of femoral metaphysis between the 5 groups(2.718+/-0.362,1.492+/-0.228,2.624+/-0.296,2.497+/-0.274,1.713+/-0.327,F=10.726,P=0.000).The typeⅠcollagen protein levels were higher in sham-operated group,nilestriol group and quercetin high-dose group compared to model group(P=0.000,P=0.000,P=0.000).There was no statistical difference in the typeⅠcollagen protein levels between quercetin low-dose group and model group(P=0.056).There was no statistical difference in the typeⅠcollagen protein levels between quercetin high-dose group and nilestriol group(P=0.217).The typeⅠcollagen protein levels were lower in quercetin low-dose group compared to quercetin high-dose group and nilestriol group(P=0.000,P=0.000).Conclusion:Quercetin has estrogen-like effects,which can effectively reduce serum osteocalcin levels and raise femur typeⅠcollagen protein levels in ovariectomized rats,and its effect is related to the dose.

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備注/Memo

備注/Memo:
2016-02-04收稿 2016-04-05修回
基金項(xiàng)目:湖北省襄陽(yáng)市重點(diǎn)科技攻關(guān)項(xiàng)目[襄科計(jì)(2012)21-5]
馮建軍 E-mail:[email protected]

更新日期/Last Update: 1900-01-01