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[1]林念慈,孫紹裘,李益亮,等.桃紅四物湯對兔自體皮片移植模型Delta-like 4表達(dá)的影響[J].中醫(yī)正骨,2015,27(11):1-7.
　LIN Nianci,SUN Shaoqiu,LI Yiliang,et al.Impact of Taohong Siwu Tang(桃紅四物湯)on Delta-like 4 expression in the autologous skin graft model of rabbits[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(11):1-7.
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桃紅四物湯對兔自體皮片移植模型Delta-like 4表達(dá)的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期數(shù):
2015年11期
頁碼:
1-7
欄目:
基礎(chǔ)研究
出版日期:
2015-11-30

文章信息/Info

Title:
Impact of Taohong Siwu Tang(桃紅四物湯)on Delta-like 4 expression in the autologous skin graft model of rabbits
作者:
林念慈1; 孫紹裘2; 李益亮2; 徐無忌2; 陳希龍2; 周昭輝2
1.廣東省江門市新會區(qū)中醫(yī)院,廣東 江門 529100;
2.湖南中醫(yī)藥大學(xué)第二附屬醫(yī)院,湖南 長沙 410005
Author(s):
LIN Nianci1; SUN Shaoqiu2; LI Yiliang2; XU Wuji2; CHEN Xilong2; ZHOU Zhaohui2
1.Traditional Chinese Medicine Hospital of Xinhui district in Jiangmen City,Jiangmen 529100,Guangdong,China
2.The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,Hunan,China
關(guān)鍵詞:
桃紅四物湯;  皮膚移植;  Notch信號通路;  兔;  動物實(shí)驗
Keywords:
Taohong Siwu Tang;  skin transplantation;  Notch Signaling Pathway;  rabbits;  animal experimentation
摘要:
目的:觀察桃紅四物湯對兔自體皮片移植模型Delta-like 4表達(dá)的影響。方法:將75只新西蘭大白兔(10～12月齡)隨機(jī)分為假手術(shù)組、模型組、低劑量組、中劑量組和高劑量組,每組15只。模型組、低劑量組、中劑量組和高劑量組動物進(jìn)行自體皮片移植手術(shù),假手術(shù)組僅在對應(yīng)區(qū)域作切口,不進(jìn)行皮下組織分離。造模術(shù)后假手術(shù)組和模型組動物以蒸餾水灌胃,低劑量組、中劑量組和高劑量組均以制備好的桃紅四物湯湯劑灌胃(每次給藥量分別為2.5 g·kg-1、5 g·kg-1、10 g·kg-1),每天2次。分別在藥物干預(yù)開始后2、5、8 d從每組隨機(jī)選取5只動物,心臟采血后處死,切取移植皮片吻合區(qū)皮片組織。采用酶聯(lián)免疫吸附實(shí)驗測定血清Delta-like 4蛋白含量; 所取皮片組織分成2份,一份制成石蠟切片,HE染色后測定血管密度,另一份采用逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)法測定Delta-like 4 mRNA含量。結(jié)果:①血清Delta-like 4蛋白含量。藥物干預(yù)開始后2 d,5組動物血清Delta-like 4蛋白含量比較,差異無統(tǒng)計學(xué)意義[(30.58±3.56)ng·mL-1,(32.31±2.66)ng·mL-1,(33.53±1.75)ng·mL-1,(36.00±1.49)ng·mL-1,(35.63±1.87)ng·mL-1,F=2.669,P=0.062]。藥物干預(yù)開始后5、8 d,5組動物血清Delta-like 4蛋白含量比較,組間差異均有統(tǒng)計學(xué)意義[(30.56±1.35)ng·mL-1,(33.52±2.50)ng·mL-1,(37.96±1.83)ng·mL-1,(48.74±2.34)ng·mL-1,(49.05±2.86)ng·mL-1,F=46.239,P=0.000;(29.26±2.20)ng·mL-1,(32.98±1.43)ng·mL-1,(35.24±1.97)ng·mL-1,(38.02±1.72)ng·mL-1,(36.06±2.50)ng·mL-1,F=8.394,P=0.000]; 模型組分別與假手術(shù)組和低劑量組比較,差異均無統(tǒng)計學(xué)意義(5 d:P=0.112,P=0.269; 8 d:P=0.340,P=0.182); 中、高劑量組血清Delta-like 4蛋白含量均高于模型組(5 d:P=0.000,P=0.000; 8 d:P=0.020,P=0.006); 中、高劑量組比較,組間差異均無統(tǒng)計學(xué)意義(P=0.862,P=0.245)。藥物干預(yù)后5 d,中、高劑量組血清Delta-like 4蛋白含量均高于低劑量組(P=0.000,P=0.000)。藥物干預(yù)開始后8 d,中、高劑量組Delta-like 4蛋白含量與低劑量組比較,差異均無統(tǒng)計學(xué)意義(P=0.105,P=0.623)。②移植皮片組織中Delta-like 4 mRNA含量。藥物干預(yù)開始后2、5、8 d,5組動物移植皮片中Delta-like 4 mRNA含量比較,組間差異均有統(tǒng)計學(xué)意義(0.26±0.03,0.29±0.04,0.34±0.06,0.42±0.03,0.39±0.04,F=7.072,P=0.001; 0.25±0.03,0.34±0.04,0.39±0.03,0.48±0.06,0.45±0.06,F=12.028,P=0.001; 0.25±0.06,0.30±0.04,0.35±0.03,0.44±0.03,0.43±0.04,F=10.813,P=0.000); 模型組分別與假手術(shù)組和低劑量組比較,差異均無統(tǒng)計學(xué)意義(2 d:P=0.452,P=0.162; 5 d:P=0.320,P=0.158; 8 d:P=0.171,P=0.219); 模型組Delta-like 4 mRNA含量低于中、高劑量組(2 d:P=0.000,P=0.001; 5 d:P=0.001,P=0.009; 8 d:P=0.001,P=0.002); 中、高劑量組比較,組間差異均無統(tǒng)計學(xué)意義(P=0.426,P=0.330,P=0.725)。藥物干預(yù)開始后2 d,中、高劑量組與低劑量組比較,差異均無統(tǒng)計學(xué)意義(P=0.111,P=0.167); 藥物干預(yù)開始后5、8 d,中、高劑量組Delta-like 4 mRNA含量均高于低劑量組(5 d:P=0.024,P=0.025; 8 d:P=0.013,P=0.028)。③移植皮片組織中血管密度。藥物干預(yù)開始后2、5 d,5組動物移植皮片中每個視野(×400)中血管數(shù)量比較,組間差異均有統(tǒng)計學(xué)意義[(5.37±0.36)個,(2.55±0.41)個,(2.83±0.24)個,(3.31±0.26)個,(3.53±0.67)個,F=4.084,P=0.014;(5.62±0.56)個,(4.25±0.68)個,(4.80±0.74)個,(5.88±0.42)個,(5.92±0.48)個,F=4.600,P=0.009]; 模型組均低于假手術(shù)組(P=0.001,P=0.011),模型組與低劑量組比較,差異均無統(tǒng)計學(xué)意義(P=0.527,P=0.268); 中、高劑量組比較,組間差異均無統(tǒng)計學(xué)意義(P=0.620,P=0.939)。藥物干預(yù)開始后2 d,中、高劑量組與模型組比較,差異均無統(tǒng)計學(xué)意義(P=0.102,P=0.131); 中、高劑量組與低劑量組比較,差異均無統(tǒng)計學(xué)意義(P=0.297,P=0.352)。藥物干預(yù)開始后5 d,中、高劑量組高于模型組(P=0.003,P=0.003); 中、高劑量組與低劑量組比較,差異均無統(tǒng)計學(xué)意義(P=0.038,P=0.033)。藥物干預(yù)開始后8 d,5組動物移植皮片中每個視野中血管數(shù)量比較,差異無統(tǒng)計學(xué)意義[(6.18±0.33)個,(5.86±0.66)個,(6.03±0.40)個,(6.27±0.53)個,(6.27±0.59)個,F=0.334,P=0.852]。結(jié)論:桃紅四物湯能上調(diào)兔自體皮片移植模型Delta-like 4的表達(dá),促進(jìn)血管新生。但本研究未能明確桃紅四物湯的劑量及作用時間與其上調(diào)Delta-like 4表達(dá)作用的關(guān)系,需要在今后的研究中進(jìn)一步探討。
Abstract:
Objective:To observe the impact of Taohong Siwu Tang(桃紅四物湯,THSWT)on Delta-like 4 expression in the autologous skin graft model of rabbits.Methods:Seventy-five New Zealand rabbits which ranged in age from 10 to 12 months were randomly divided into sham-operated group,model group,low-dose group,middle-dose group and high-dose group,15 cases in each group.The autologous skin transplantation surgery were performed on rabbits in model group,low-dose group,middle-dose group and high-dose group,while sham operation were performed on rabbits in sham-operated group.After the modeling operation,the rabbits in sham-operated group and model group were intragastric administrated with distilled water; while the others in low-dose group,middle-dose group and high-dose group were intragastric administrated with the prepared THSWT in dosages of 2.5,5 and 10 g/kg at a time,twice a day.Five rabbits were randomly selected from each group and the blood samples were obtained from the heart at 2,5 and 8 days after the beginning of the drug intervention respectively.The rabbits were executed and their anastomotic skin graft tissues were fetched out.The serum content of Delta-like 4 protein were measured by using enzyme-linked immunosorbent assay(ELISA).The acquired skin tissues were divided into 2 parts,and one of them were maded into paraffin sections and the blood vessel density were measured after HE staining,while the other portion were applied to measure the Delta-like 4 mRNA level by using reverse transcription-polymerase chain reaction(RT-PCR).Results:There was no statistical difference in serum content of Delta-like 4 protein between the 5 groups at 2 days after the beginning of the drug intervention(30.58+/-3.56,32.31+/-2.66,33.53+/-1.75,36.00+/-1.49,35.63+/-1.87 ng/ml,F=2.669,P=0.062).There was statistical difference in serum content of Delta-like 4 protein between the 5 groups at 5 and 8 days after the beginning of the drug intervention(30.56+/-1.35,33.52+/-2.50,37.96+/-1.83,48.74+/-2.34,49.05+/-2.86 ng/ml,F=46.239,P=0.000; 29.26+/-2.20,32.98+/-1.43,35.24+/-1.97,38.02+/-1.72,36.06+/-2.50 ng/ml,F=8.394,P=0.000).There was no statistical difference in serum content of Delta-like 4 protein between model group and sham-operated group and between model group and low-dose group(5d:P=0.112,P=0.269; 8d:P=0.340,P=0.182).The serum content of Delta-like 4 protein were higher in middle-dose group and high-dose group compared to model group(5d:P=0.000,P=0.000; 8d:P=0.020,P=0.006).There was no statistical difference in serum content of Delta-like 4 protein between middle-dose group and high-dose group(P=0.862,P=0.245).The serum content of Delta-like 4 protein were higher in middle-dose group and high-dose group compared to low-dose group at 5 days after the beginning of the drug intervention(P=0.000,P=0.000).There was no statistical difference in serum content of Delta-like 4 protein between middle-dose group and low-dose group and between high-dose group and low-dose group at 8 days after the beginning of the drug intervention(P=0.105,P=0.623).There was statistical difference in mRNA level of Delta-like 4 in skin graft between the 5 groups at 2,5 and 8 days after the beginning of the drug intervention(0.26+/-0.03,0.29+/-0.04,0.34+/-0.06,0.42+/-0.03,0.39+/-0.04,F=7.072,P=0.001; 0.25+/-0.03,0.34+/-0.04,0.39+/-0.03,0.48+/-0.06,0.45+/-0.06,F=12.028,P=0.001; 0.25+/-0.06,0.30+/-0.04,0.35+/-0.03,0.44+/-0.03,0.43+/-0.04,F=10.813,P=0.000).There was no statistical difference in Delta-like 4 mRNA level between model group and sham-operated group and between model group and low-dose group(2 d:P=0.452,P=0.162; 5 d:P=0.320,P=0.158; 8 d:P=0.171,P=0.219).The Delta-like 4 mRNA level were lower in model group compared to middle-dose group and high-dose group(2 d:P=0.000,P=0.001; 5 d:P=0.001,P=0.009; 8 d:P=0.001,P=0.002).There was no statistical difference in Delta-like 4 mRNA level between middle-dose group and high-dose group(P=0.426,P=0.330,P=0.725).There was no statistical difference in Delta-like 4 mRNA level between middle-dose group and low-dose group and between high-dose group and low-dose group at 2 days after the beginning of the drug intervention(P=0.111,P=0.167).The Delta-like 4 mRNA level was higher in middle-dose group and high-dose group compared to low-dose group at 5 and 8 days after the beginning of the drug intervention(5 d:P=0.024,P=0.025; 8 d:P=0.013,P=0.028).There was statistical difference in the number of blood vessels in skin graft under the optical microscope(×400)between the 5 groups at 2 and 5 days after the beginning of the drug intervention(5.37+/-0.36,2.55+/-0.41,2.83+/-0.24,3.31+/-0.26,3.53+/-0.67,F=4.084,P=0.014; 5.62+/-0.56,4.25+/-0.68,4.80+/-0.74,5.88+/-0.42,5.92+/-0.48,F=4.600,P=0.009).The number of blood vessels was less in model group compared to sham-operated group(P=0.001,P=0.011),and there was no statistical difference in the number of blood vessels between model group and low-dose group(P=0.527,P=0.268)and between middle-dose group and high-dose group(P=0.620,P=0.939).At 2 days after the beginning of the drug intervention,there was no statistical difference in the number of blood vessels between middle-dose group and model group(P=0.102)and between high-dose group and model group(P=0.131)and between middle-dose group and low-dose group(P=0.297)and between high-dose group and low-dose group(P=0.352).At 5 days after the beginning of the drug intervention,the number of blood vessels was more in middle-dose group and high-dose group compared to model group(P=0.003,P=0.003),while no statistical difference was found between middle-dose group and low-dose group and between high-dose group and low-dose group(P=0.038,P=0.033).At 8 days after the beginning of the drug intervention,there was no statistical difference in the number of blood vessels in skin graft under the optical microscope(×400)between the 5 groups(6.18+/-0.33,5.86+/-0.66,6.03+/-0.40,6.27+/-0.53,6.27+/-0.59,F=0.334,P=0.852).Conclusion:THSWT can up-regulate Delta-like 4 expression and promote angiogenesis in autologous skin graft model of rabbits.However,we cann't clarify the relationship between the role of THSWT in up-regulating Delta-like 4 expression and its dose and action time,which need to be further studied.

參考文獻(xiàn)/References:

[1] Wang H,Huang X,Zhang J,et al.The expression of VEGF and Dll4/Notch pathway molecules in ovarian cancer[J].Clin Chim Acta,2014,436(9):243-248.
[2] Koukourakis MI,Giatromanolaki A,Sivridis E,et al.High DLL4 expression in tumour-associated vessels predicts for favorable radiotherapy outcome in locally advanced squamous cell head-neck cancer(HNSCC)[J].Angiogenesis,2013,16(2):343-351.
[3] 林念慈,孫紹裘,李益亮.Notch信號通路配體對血管新生的影響[J].中醫(yī)正骨,2015,27(5):70-72.
[4] García-Pascual CM,Ferrero H,Zimmermann RC,et al.Inhibition of Delta-like 4 mediated signaling induces abortion in mice due to deregulation of decidual angiogenesis[J].Placenta,2014,35(7):501-508.
[5] Niessen K,Zhang G,Ridgway JB,et al.The notch1-Dll4 signaling pathway regulates mouse postnatal lymphatic development[J].Blood,2011,118(7):1989-1997.
[6] Nus M,Macgrogan D,Martínez-Poveda B,et al.Diet-induced aortic valve disease in mice haploinsufficient for the Notch pathway effector RBPJK/CSL[J].Arterioscler Thromb Vasc Biol,2011,31(7):1580-1588.
[7] 孫紹裘,李益亮,陳孟交,等.桃紅四物丸促兔自體皮片移植術(shù)后不同時限VEGF表達(dá)的研究[J].湖南中醫(yī)雜志,2013,29(3):115-116.
[8] Bridges E,Oon CE,Harris A.Notch regulation of tumor angiogenesis[J].Future Oncol,2011,7(4):569-588.
[9] Zhang J,Ye J,Ma D,et al.Cross-talk between leukemic and endothelial cells promotes angiogenesis by VEGF activation of the Notch/Dll4 pathway[J].Carcinogenesis,2013,34(3):667-677. 中醫(yī)正骨2015年11月第27卷第11期 J Trad Chin Orthop Trauma,2015,Vol.27,No.11(總807) (總808)中醫(yī)正骨2015年11月第27卷第11期 J Trad Chin Orthop Trauma,2015,Vol.27,No.11
[10] Abbas OL,Borman H,Terzi YK,et al.The notch pathway is a critical regulator of angiogenesis in a skin model of ischemia[J].Vasc Med,2015,20(3):205-211.
[11] Thomas JL,Baker K,Han J,et al.Interactions between VEGFR and Notch signaling pathways in endothelial and neural cells[J].Cell Mol Life Sci,2013,70(10):1779-1792.
[12] García-Pascual CM,Zimmermann RC,Ferrero H,et al.Delta-like ligand 4 regulates vascular endothelial growth factor receptor 2-driven luteal angiogenesis through induction of a tip/stalk phenotype in proliferating endothelial cells[J].Fertil Steril,2013,100(6):1768-1776.
[13] Camelo S,Raoul W,Lavalette S,et al.Delta-like 4 inhibits choroidal neovascularization despite opposing effects on vascular endothelium and macrophages[J].Angiogenesis,2012,15(4):609-622.
[14] Schulz GB,Wieland E,Wüstehube-Lausch J,et al.Cerebral cavernous malformation-1 protein controls DLL4-Notch3 signaling between the endothelium and pericytes[J].Stroke,2015,46(5):1337-1343.
[15] 李翊,彭代銀.桃紅四物湯的藥理學(xué)研究進(jìn)展[J].安徽醫(yī)藥,2011,15(5):529-531.
[16] 劉竹青,尹登科,韓嵐,等.桃紅四物湯含藥血清對過氧化氫損傷的人臍靜脈內(nèi)皮細(xì)胞的保護(hù)作用[J].中國中藥雜志,2013,38(3):402-406.
[17] 董大立,周昭輝,陳希龍,等.桃紅四物湯促進(jìn)隨意皮瓣血管再生機(jī)制的探討[J].湖南中醫(yī)雜志,2013,29(5):117-118.
[18] 石毅,趙小紅,許云云,等.Notch信號在血流動力學(xué)對血管發(fā)育與疾病中的作用[J].解剖學(xué)研究,2011,33(1):59-63.
[19] Watson O,Novodvorsky P,Gray C,et al.Blood flow suppresses vascular Notch signalling via dll4 and is required for angiogenesis in response to hypoxic signalling[J].Cardiovasc Res,2013,100(2):252-261.

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備注/Memo

備注/Memo:
2015-07-16收稿 2015-10-07修回
基金項目:國家自然科學(xué)基金項目(81373658)
通訊作者:孫紹裘 E-mail:[email protected]
更新日期/Last Update: 2015-11-30