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[1]徐西林,趙永蘭,張曉峰,等.活骨注射液髖關(guān)節(jié)腔灌注對兔股骨頭壞死模型 血管內(nèi)皮生長因子表達(dá)的動(dòng)態(tài)影響[J].中醫(yī)正骨,2015,27(08):1-6.
　XU Xilin,ZHAO Yonglan,ZHANG Xiaofeng,et al.Dynamic effect of intra-articular hip injection of Huogu injection on the expression of vascular endothelial growth factor in rabbit models with femur head necrosis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(08):1-6.
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活骨注射液髖關(guān)節(jié)腔灌注對兔股骨頭壞死模型 血管內(nèi)皮生長因子表達(dá)的動(dòng)態(tài)影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期數(shù):
2015年08期
頁碼:
1-6
欄目:
基礎(chǔ)研究
出版日期:
2015-08-30

文章信息/Info

Title:
Dynamic effect of intra-articular hip injection of Huogu injection on the expression of vascular endothelial growth factor in rabbit models with femur head necrosis
作者:
徐西林1; 趙永蘭1; 張曉峰1; 王智1; 韓雪松2; 呂航1
1.黑龍江中醫(yī)藥大學(xué)附屬第二醫(yī)院,黑龍江 哈爾濱 150001;
2.黑龍江省大慶市人民醫(yī)院,黑龍江 大慶 163316
Author(s):
XU Xilin1; ZHAO Yonglan1; ZHANG Xiaofeng1; WANG Zhi1; HAN Xuesong2; LYU Hang1
1.The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150001,Heilongjiang,China
2.The People's Hospital of Daqing City,Daqing 163316,Heilongjiang,China
關(guān)鍵詞:
股骨頭壞死;  血管內(nèi)皮生長因子類;  活骨注射液;  模型; 動(dòng)物;  動(dòng)物實(shí)驗(yàn)
Keywords:
femur head necrosis;  vascular endothelial growth factors;  Huogu injection;  models; animal;  animal experimentation
摘要:
目的:觀察活骨注射液髖關(guān)節(jié)腔灌注對兔股骨頭壞死模型血管內(nèi)皮生長因子(vascular endothelial growth factor,VEGF)表達(dá)的動(dòng)態(tài)影響。方法:將90只新西蘭大白兔隨機(jī)分為對照組、模型組和實(shí)驗(yàn)組,每組30只。對模型組和實(shí)驗(yàn)組動(dòng)物采用液氮冷凍法進(jìn)行股骨頭壞死造模; 造模成功后,分別以生理鹽水和活骨注射液進(jìn)行髖關(guān)節(jié)腔灌注。對照組不進(jìn)行任何干預(yù)。藥物干預(yù)開始后1、3、6、9、12周時(shí),分別從各組隨機(jī)選取6只動(dòng)物處死后取出股骨頭,采用免疫組化法和實(shí)時(shí)定量PCR法檢測VEGF表達(dá)情況。結(jié)果:藥物干預(yù)后各時(shí)點(diǎn),3組動(dòng)物股骨頭VEGF蛋白表達(dá)量比較,組間差異均有統(tǒng)計(jì)學(xué)意義(490.56±60.62,661.99±64.51,796.09±86.17,F=27.670,P=0.000; 501.41±58.85,781.71±87.32,854.71±76.97,F=36.810,P=0.000; 489.33±53.19,592.42±117.35,905.51±107.95,F=29.930,P=0.000; 498.96±40.37,571.31±111.38,1158.20±147.35,F=65.820,P=0.000; 500.47±33.67,508.01±138.76,1528.20±146.09,F=150.770,P=0.000)。對照組各時(shí)點(diǎn)股骨頭VEGF蛋白表達(dá)量均低于實(shí)驗(yàn)組(P=0.000,P=0.000,P=0.000,P=0.000,P=0.000); 藥物干預(yù)后1、3周時(shí)對照組股骨頭VEGF蛋白表達(dá)量均低于模型組(P=0.001,P=0.000); 藥物干預(yù)后1、6、9、12周時(shí)模型組股骨頭VEGF蛋白表達(dá)量均低于實(shí)驗(yàn)組(P=0.005,P=0.000,P=0.000,P=0.000); 其余各時(shí)點(diǎn)組間兩兩比較,差異均無統(tǒng)計(jì)學(xué)意義。藥物干預(yù)后各時(shí)點(diǎn),3組動(dòng)物股骨頭VEGF mRNA表達(dá)量比較,組間差異均有統(tǒng)計(jì)學(xué)意義(1.000±0.000,1.313±0.380,1.546±0.204,F=9.650,P=0.001; 1.000±0.000,1.412±0.345,1.750±0.236,F=19.361,P=0.000; 1.000±0.000,1.235±0.095,1.807±0.211,F=35.216,P=0.000; 1.000±0.000,1.234±0.250,2.691±0.289,F=137.743,P=0.000; 1.000±0.000,1.113±0.180,3.106±0.287,F=293.245,P=0.000)。對照組各時(shí)點(diǎn)股骨頭VEGF mRNA表達(dá)量均低于實(shí)驗(yàn)組(P=0.000,P=0.000,P=0.000,P=0.000,P=0.000); 藥物干預(yù)后1、3、6、9周時(shí)對照組股骨頭VEGF mRNA表達(dá)量均低于模型組(P=0.020,P=0.003,P=0.027,P=0.046); 藥物干預(yù)后3、6、9、12周時(shí)模型組股骨頭VEGF mRNA表達(dá)量均低于實(shí)驗(yàn)組(P=0.011,P=0.000,P=0.000,P=0.000); 其余各時(shí)點(diǎn)組間兩兩比較,差異均無統(tǒng)計(jì)學(xué)意義。結(jié)論:活骨注射液髖關(guān)節(jié)腔灌注可持續(xù)促進(jìn)兔股骨頭壞死模型壞死股骨頭局部VEGF表達(dá),這可能是其治療股骨頭壞死的主要作用機(jī)制之一。
Abstract:
Objective:To observe the dynamic effect of intra-articular hip injection of Huogu injection on the expression of vascular endothelial growth factor(VEGF)in rabbit models with osteonecrosis of the femoral head(ONFH).Methods:Ninety New Zealand white rabbits were randomly divided into control group,model group and experimental group,30 cases in each group.The rabbits in model group and experimental group were administrated with liquid-nitrogen refrigeration to build ONFH models,and then they were intra-articular injected with normal saline and Huogu injection respectively in the hip,while the rabbits in the control group were not be treated.At 1,3,6,9 and 12 weeks after the beginning of drug intervention,6 rabbits were randomly selected from each group respectively and were executed,then their femoral heads were fetched out and were sectioned for detecting the expression of VEGF by immunohistochemical method and real-time quantitative PCR method.Results:There was statistical difference in the expression of VEGF protein in femoral head between the 3 groups at each timepoint after the beginning of drug intervention(490.56+/-60.62,661.99+/-64.51,796.09+/-86.17,F=27.670,P=0.000; 501.41+/-58.85,781.71+/-87.32,854.71+/-76.97,F=36.810,P=0.000; 489.33+/-53.19,592.42+/-117.35,905.51+/-107.95,F=29.930,P=0.000; 498.96+/-40.37,571.31+/-111.38,1158.20+/-147.35,F=65.820,P=0.000; 500.47+/-33.67,508.01+/-138.76,1528.20+/-146.09,F=150.770,P=0.000).The expression of VEGF protein in femoral head was lower in control group compared to experimental group at each timepoint after the beginning of drug intervention(P=0.000,P=0.000,P=0.000,P=0.000,P=0.000).The expression of VEGF protein in femoral head were lower in control group compared to model group at 1 and 3 weeks after the beginning of drug intervention(P=0.001,P=0.000)and were lower in model group compared to experimental group at 1,6,9 and 12 weeks after the beginning of drug intervention(P=0.005,P=0.000,P=0.000,P=0.000),while there was no statistical difference between the paired groups at other timepoints.There was statistical difference in VEGF mRNA expression in the femoral head between the 3 groups at each timepoint after the beginning of drug intervention(1.000+/-0.000,1.313+/-0.380,1.546+/-0.204,F=9.650,P=0.001; 1.000+/-0.000.1.412+/-0.345,1.750+/-0.236,F=19.361,P=0.000; 1.000+/-0.000,1.235+/-0.095,1.807+/-0.211,F=35.216,P=0.000; 1.000+/-0.000,1.234+/-0.250,2.691+/-0.289,F=137.743,P=0.000; 1.000+/-0.000,1.113+/-0.180,3.106+/-0.287,F=293.245,P=0.000).The VEGF mRNA expression in the femoral head was lower in control group compared to experimental group at each timepoint after the beginning of drug intervention(P=0.000,P=0.000,P=0.000,P=0.000,P=0.000),and were lower in control group compared to model group at 1,3,6 and 9 weeks after the beginning of drug intervention(P=0.020,P=0.003,P=0.027,P=0.046),and were lower in model group compared to experimental group at 3,6,9 and 12 weeks after the beginning of drug intervention(P=0.011,P=0.000,P=0.000,P=0.000),while there was no statistical difference between the paired groups at other timepoints.Conclusion:Intra-articular hip injection of Huogu injection can promote the expression of VEGF in necrotic femoral head of rabbit ONFH model,which may be one of the mechanisms of action for treatment of ONFH.

參考文獻(xiàn)/References:

[1] 陳衛(wèi)衡.股骨頭壞死的診治誤區(qū)與對策[J].中醫(yī)正骨,2013,25(3):3-5.
[2] 徐西林,張曉峰,段洪超,等.活骨注射液髖關(guān)節(jié)腔注射對兔VEGF mRNA表達(dá)影響的研究[J].中醫(yī)藥信息,2011,28(5):64-66.
[3] 沈霖,林燕萍,王擁軍.骨傷科實(shí)驗(yàn)研究[M].北京:北京科學(xué)技術(shù)出版社,2005:434.
[4] 凌峰,王燕,熊龍,等.股骨頭壞死病因發(fā)病機(jī)制的研究進(jìn)展[J].實(shí)用臨床醫(yī)學(xué),2011,12(11):132-134.
[5] Kerachian MA,Harvey EJ,Cournoyer D,et al.Avascular necrosis of the femoral head:vascular hypotheses[J].Endothelium,2006,13(4):237-244.
[6] 劉春輝,趙德偉,李志剛,等.液氮冷凍犬股骨頭壞死過程中細(xì)胞因子和組織學(xué)的動(dòng)態(tài)變化[J].中國骨與關(guān)節(jié)外科,2011,4(2):135-140.
[7] 于潼.硬化帶在股骨頭壞死塌陷作用中的研究進(jìn)展[J].醫(yī)學(xué)綜述,2015,21(1):84-86.
[8] 周正新,孫志濤,丁鍔.骨蝕寧膠囊對激素性股骨頭壞死患者凝血機(jī)制的影響[J].中醫(yī)正骨,2013,25(3):31-34.
[9] 蔡源源,李光早.血管內(nèi)皮生長因子的調(diào)控及其作用研究進(jìn)展[J].組織工程與重建外科雜志,2011,7(1):51-54.
[10] Feng L,Wu H,E L,et al.Effects of vascular endothelial growth factor 165 on bone tissue engineering[J].PLoS One,2013,8(12):e82945.
[11] Turner N,Grose R.Fibroblast growth factor signalling:from development to cancer[J].Nat Rev Cancer,2010,10(2):116-129.
[12] Pugh CW,Ratcliffe PJ.Regulation of angiogenesis by hypoxia:role of the HIF system[J].Nat Med,2003,9(6):677-684.
[13] Ishibashi H,Shiratuchi T,Nakagawa K,et al.Hypoxia-induced angiogenesis of cultured human salivary gland carcinoma cells enhances vascular endothelial growth factor production and basic fibroblast growth factor release[J].Oral Oncol,2001,37(1):77-83.
[14] Ziemer LS,Koch CJ,Maity A,et al.Hypoxia and VEGF mRNA expression in human tumors[J].Neoplasia,2001,3(6):500-508.
[15] 崔永鋒,王利明,肖魯偉,等.股骨頭壞死修復(fù)過程中斷的原因分析[J].中華中醫(yī)藥雜志,2009,24(11):1473-1476.
[16] 顏冰,王和鳴.活血補(bǔ)腎湯對兔激素性股骨頭壞死VEGF的影響[J].中國中醫(yī)骨傷科雜志,2010,18(5):1-4.

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備注/Memo

備注/Memo:
2015-03-27收稿 2015-05-27修回
基金項(xiàng)目:國家自然科學(xué)基金項(xiàng)目(81173276); 哈爾濱市科技創(chuàng)新人才項(xiàng)目(2011RFXYS070)
通訊作者:張曉峰 E-mail:zxfeng9919@ 163.com
更新日期/Last Update: 2015-08-30