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[1]齊新宇,向黎黎,熊輝,等.基于高蛋白飲食建立雞痛風(fēng)性關(guān)節(jié)炎模型的量效關(guān)系研究[J].中醫(yī)正骨,2015,27(02):1-6.
　QI Xinyu,XIANG Lili,XIONG Hui,et al.A dose-effect relationship study of gouty arthritis chicken model built by high-protein diet[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(02):1-6.
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基于高蛋白飲食建立雞痛風(fēng)性關(guān)節(jié)炎模型的量效關(guān)系研究 ()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期數(shù):
2015年02期
頁(yè)碼:
1-6
欄目:
基礎(chǔ)研究
出版日期:
2015-02-10

文章信息/Info

Title:
A dose-effect relationship study of gouty arthritis chicken model built by high-protein diet
作者:
齊新宇1; 向黎黎1; 熊輝2; 李騰龍1; 周彪1; 郭玉星1;  陸小龍2
1.湖南中醫(yī)藥大學(xué),湖南 長(zhǎng)沙 410208;
2.湖南中醫(yī)藥大學(xué)第二附屬醫(yī)院,湖南 長(zhǎng)沙 410005
Author(s):
QI Xinyu1; XIANG Lili1; XIONG Hui2; LI Tenglong1; ZHOU Biao1; GUO Yuxing1; LU Xiaolong2
1.Hunan University of Traditional Chinese Medicine,Changsha 410007,Hunan,China;
2.The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,Hunan,China
關(guān)鍵詞:
關(guān)節(jié)炎; 痛風(fēng)性;  疾病模型; 動(dòng)物;  尿酸;  動(dòng)物實(shí)驗(yàn)
Keywords:
arthritis; gouty;  disease models; animal;  uric acid;  animal experimentation
摘要:
目的:探討基于高蛋白飲食建立雞痛風(fēng)性關(guān)節(jié)炎模型時(shí)飼料蛋白含量與造模效果之間的量效關(guān)系。方法:將160只30日齡雄 性湘黃雞隨機(jī)分為對(duì)照組、模型1組、模型2組和模型3組,每組40只,分別以蛋白含量為19.8%、40.19%、50%和55.2%的飼料喂養(yǎng), 連續(xù)喂養(yǎng)21 d。分別于造模開(kāi)始后1、7、14、21 d在每組隨機(jī)選出10只雞,分別觀察其一般狀態(tài)、踝關(guān)節(jié)周徑和血尿酸含量; 隨 后處死動(dòng)物,分離出左側(cè)踝關(guān)節(jié)滑膜,觀察其滑膜組織形態(tài)。結(jié)果:①一般狀態(tài)。除造模開(kāi)始后1 d時(shí)外,其余各時(shí)點(diǎn)4組一般狀態(tài) 評(píng)分比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=65.721,P=0.271; F=70.634,P=0.013; F=59.448,P=0.001; F=73.124,P=0.001)。造模開(kāi) 始后7、14、21 d時(shí),對(duì)照組的評(píng)分均高于模型1組、模型2組和模型3組(P=0.021,P=0.013,P=0.020; P=0.001,P=0.001,P=0.001; P=0.000,P=0.000,P=0.000),模型2組的評(píng)分均低于模型1組和模型3組(P=0.031,P=0.025; P=0.001,P=0.001; P=0.000,P=0.000),模型1組與模型3組比較差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.125,P=0.112,P=0.141)。②踝關(guān)節(jié)周徑。除造模開(kāi)始后1 d 時(shí)外,其余各時(shí)點(diǎn)4組踝關(guān)節(jié)周徑比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=115.172,P=0.162; F=124.445,P=0.014; F=121.237,P=0.002; F=119.881,P=0.002)。造模開(kāi)始后7、14、21 d時(shí),對(duì)照組的踝關(guān)節(jié)周徑均低于模型1組、模型2組和模型3 組(P=0.033,P=0.024,P=0.022; P=0.012,P=0.010,P=0.011; P=0.001,P=0.000,P=0.001),模型2組的踝關(guān)節(jié)周徑均大于模型1組 和模型3組(P=0.032,P=0.027; P=0.012,P=0.011; P=0.001,P=0.001),模型1組與模型3組比較差異均無(wú)統(tǒng)計(jì)學(xué)意義 (P=0.141,P=0.126,P=0.134)。③血尿酸含量。除造模開(kāi)始后1 d時(shí)外,其余各時(shí)點(diǎn)4組血尿酸含量比較,組間差異均有統(tǒng)計(jì)學(xué)意義 (F=35.361,P=0.273; F=52.371,P=0.021; F=56.734,P=0.004; F=48.336,P=0.003)。造模開(kāi)始后7、14、21 d時(shí),對(duì)照組的血尿 酸含量均低于模型1組、模型2組和模型3組(P=0.021,P=0.022,P=0.019; P=0.011,P=0.004,P=0.010; P=0.003,P=0.001,P=0.003),模型2組的血尿酸含量均高于模型1組和模型3組(P=0.031,P=0.033; P=0.011,P=0.011; P=0.002,P=0.001),模型1組與模型3組比較差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.138,P=0.220,P=0.341)。④滑膜組織形態(tài)。除造模開(kāi)始后1 d時(shí)外,其余各時(shí)點(diǎn)4組滑膜中血管數(shù)量比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=26.772,P=0.183; F=32.176,P=0.022; F=33.672,P=0.003; F=41.351,P=0.001)。造模開(kāi)始后7、14、21 d時(shí),對(duì)照組滑膜中血管數(shù)量均少于模型1組、模型2組和模型3 組(P=0.022,P=0.022,P=0.020; P=0.016,P=0.011,P=0.014; P=0.005,P=0.001,P=0.003),模型2組滑膜中血管數(shù)量均多于模型1 組和模型3組(P=0.016,P=0.012; P=0.010,P=0.011; P=0.001,P=0.001),模型1組與模型3組比較差異均無(wú)統(tǒng)計(jì)學(xué)意義 (P=0.243,P=0.251,P=0.320)。除造模開(kāi)始后1 d時(shí)外,其余各時(shí)點(diǎn)4組滑膜中中性粒細(xì)胞數(shù)量比較,組間差異均有統(tǒng)計(jì)學(xué)意義 (F=32.347,P=0.311; F=43.561,P=0.014; F=42.361,P=0.000; F=51.745,P=0.000)。造模開(kāi)始后7、14、21 d時(shí),對(duì)照組滑膜中 中性粒細(xì)胞數(shù)量均少于模型1組、模型2組和模型3組(P=0.025,P=0.018,P=0.021; P=0.014,P=0.010,P=0.012; P=0.004,P=0.002,P=0.004),模型2組滑膜中中性粒細(xì)胞數(shù)量均多于模型1組和模型3組(P=0.027,P=0.022; P=0.015,P=0.013; P=0.002,P=0.003),模型1組與模型3組比較差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.220,P=0.351,P=0.117)。結(jié)論:通過(guò)高蛋白飲食誘導(dǎo)可獲得 雞痛風(fēng)性關(guān)節(jié)炎模型,其中以蛋白含量為50%的飲食造模效果最好。
Abstract:
Objective:To explore the dose-effect relationship between feedstuff protein content and gouty arthritis chicken models built by high-protein diet.Methods:One hundred and sixty 30-day-old male XIANGHUANG chickens were randomly divided into control group,model group 1,model group 2 and model group 3,40 cases in each group.The chickens were fed for consecutive 21 days with feedstuff which protein content was 19.8%,40.19%,50% and 55.2% respectively.Ten chickens were randomly selected from each group at 1,7,14 and 21 days after the beginning of modeling respectively; and the general state,ankle circumference and blood uric acid levels were detected.Then the chickens were executed,and the left ankle synovium were separated for observing the synovial tissue morphology.Results:There was statistical difference in general state scores between the 4 groups except at one day after the beginning of modeling(F=65.721,P=0.271; F=70.634,P=0.013; F=59.448,P=0.001; F=73.124,P=0.001).The scores of control group were higher than that of model group 1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling(P=0.021,P=0.013,P=0.020; P=0.001,P=0.001,P=0.001; P=0.000,P=0.000,P=0.000); and the scores of model group 2 were lower than that of model group 1 and model group 3(P=0.031,P=0.025; P=0.001,P=0.001; P=0.000,P=0.000); there was no statistical difference in general state scores between model group 1 and model group 3(P=0.125,P=0.112,P=0.141).There was statistical difference in ankle circumference between the 4 groups except at one day after the beginning of modeling(F=115.172,P=0.162; F=124.445,P=0.014; F=121.237,P=0.002; F=119.881,P=0.002).The ankle circumference of control group were less than that of model group 1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling (P=0.033,P=0.024,P=0.022; P=0.012,P=0.010,P=0.011; P=0.001,P=0.000,P=0.001); and the ankle circumference of model group 2 were greater than that of model group 1 and model group 3(P=0.032,P=0.027; P=0.012,P=0.011; P=0.001,P=0.001); there was no statistical difference in ankle circumference between model group 1 and model group 3(P=0.141,P=0.126,P=0.134).There was statistical difference in blood uric acid levels between the 4 groups except at one day after the beginning of modeling(F=35.361,P=0.273; F=52.371,P=0.021; F=56.734,P=0.004; F=48.336,P=0.003).The blood uric acid levels of control group were lower than that of model group 1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling(P=0.021,P=0.022,P=0.019; P=0.011,P=0.004,P=0.010; P=0.003,P=0.001,P=0.003); and the blood uric acid levels of model group 2 were higher than that of model group 1 and model group 3(P=0.031,P=0.033; P=0.011,P=0.011; P=0.002,P=0.001); there was no statistical difference in blood uric acid levels between model group 1 and model group 3 (P=0.138,P=0.220,P=0.341).There was statistical difference in the number of blood vessels in synovium between the 4 groups except at one day after the beginning of modeling(F=26.772,P=0.183; F=32.176,P=0.022; F=33.672,P=0.003; F=41.351,P=0.001).The number of blood vessels in synovium of control group was fewer than that of model group 1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling (P=0.022,P=0.022,P=0.020; P=0.016,P=0.011,P=0.014; P=0.005,P=0.001,P=0.003); and the number of blood vessels in synovium of model group 2 was more than that of model group 1 and model group 3(P=0.016,P=0.012; P=0.010,P=0.011; P=0.001,P=0.001); there was no statistical difference in the number of blood vessels in synovium between model group 1 and model group 3(P=0.243,P=0.251,P=0.320).There was statistical difference in the number of neutrophils in synovium between the 4 groups except at one day after the beginning of modeling (F=32.347,P=0.311; F=43.561,P=0.014; F=42.361,P=0.000; F=51.745,P=0.000).The number of neutrophils in synovium of control group was fewer than that of model group 1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling(P=0.025,P=0.018,P=0.021; P=0.014,P=0.010,P=0.012; P=0.004,P=0.002,P=0.004); and the number of neutrophils in synovium of model group 2 was more than that of model group 1 and model group 3 (P=0.027,P=0.022; P=0.015,P=0.013; P=0.002,P=0.003); there was no statistical difference in the number of neutrophils in synovium between model group 1 and model group 3(P=0.220,P=0.351,P=0.117).Conclusion:The gouty arthritis chicken model can be built by high-protein diet induction,and the diet which protein content is 50% has the best induction effect.

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備注/Memo

備注/Memo:
2014-12-28收稿 2015-01-29修回
基金項(xiàng)目:湖南省自然科學(xué)基金項(xiàng)目(13JJ3102)
通訊作者:熊輝 E-mail:[email protected]
更新日期/Last Update: 2015-02-25