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[1]胡鋼鋒,肖魯偉,童培建.關(guān)節(jié)液代謝組學(xué)在類風(fēng)濕關(guān)節(jié)炎診斷 及寒熱證候分型中的應(yīng)用[J].中醫(yī)正骨,2015,27(01):5-8.
　HU Gangfeng,XIAO Luwei,TONG Peijian.Application of joint fluid metabolomics to diagnosis and COLD-HEAT SYMPTOM COMPLEX typing in patients with rheumatoid arthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(01):5-8.
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關(guān)節(jié)液代謝組學(xué)在類風(fēng)濕關(guān)節(jié)炎診斷 及寒熱證候分型中的應(yīng)用()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期數(shù):
2015年01期
頁碼:
5-8
欄目:
臨床研究
出版日期:
2015-01-15

文章信息/Info

Title:
Application of joint fluid metabolomics to diagnosis and COLD-HEAT SYMPTOM COMPLEX typing in patients with rheumatoid arthritis
作者:
胡鋼鋒1; 肖魯偉2; 童培建3
1.浙江省杭州市蕭山區(qū)第一人民醫(yī)院,浙江 杭州 311201;
2.浙江中醫(yī)藥大學(xué),浙江 杭州 310053;
3.浙江省中醫(yī)院,浙江 杭州 310006
Author(s):
HU Gangfeng1; XIAO Luwei2; TONG Peijian3
1.The First People's Hospital of Xiaoshan district in Hangzhou,Hangzhou 311201,Zhejiang,China.
2.Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China.
3.Zhejiang Provincial hospital of TCM,Hangzhou 310006,Zhejiang,China.
關(guān)鍵詞:
關(guān)節(jié)炎; 類風(fēng)濕;  診斷;  證候;  濕熱;  寒濕;  代謝組學(xué);  主成分分析
Keywords:
arthritis; pheumatoid;  diagnosis;  symptom complex;  dampness-heat;  cold-dampness;  metabolomics;  principal component analysis
摘要:
目的:探討關(guān)節(jié)液代謝組學(xué)在類風(fēng)濕關(guān)節(jié)炎診斷及寒熱證候分型中的價(jià)值。方法:采集12例類風(fēng)濕關(guān)節(jié)炎患者(寒濕阻絡(luò)證6例,濕熱阻絡(luò)證6例)和7名健康志愿者的膝關(guān)節(jié)關(guān)節(jié)液,對其進(jìn)行氣相色譜-質(zhì)譜分析,并對選定的主要色譜峰的氣相色譜-質(zhì)譜分析數(shù)據(jù)進(jìn)行主成分分析。結(jié)果:共選定23個(gè)主要色譜峰為共有峰描述關(guān)節(jié)液化學(xué)組成特征,健康志愿者和類風(fēng)濕關(guān)節(jié)炎患者的氣相色譜-質(zhì)譜分析總流離子圖總體特征基本相同。經(jīng)主成分分析將23個(gè)描述關(guān)節(jié)液化學(xué)組成特征的色譜峰綜合成4個(gè)主成分。對所有被測試者的關(guān)節(jié)液樣品進(jìn)行主成分分析,在第3和第4主成分得分圖中類風(fēng)濕關(guān)節(jié)炎患者的關(guān)節(jié)液樣品得分分布集中,健康志愿者關(guān)節(jié)液樣品得分分布較為分散。單獨(dú)對類風(fēng)濕關(guān)節(jié)炎患者的關(guān)節(jié)液樣品進(jìn)行主成分分析,在第3和第4主成分得分圖中濕熱阻絡(luò)證患者和寒濕阻絡(luò)證患者的關(guān)節(jié)液樣品得分分布均較為集中。結(jié)論:膝關(guān)節(jié)液的代謝組數(shù)據(jù)能準(zhǔn)確區(qū)分類風(fēng)濕關(guān)節(jié)炎患者和健康志愿者,以及類風(fēng)濕關(guān)節(jié)炎濕熱阻絡(luò)證和寒濕阻絡(luò)證患者,為類風(fēng)濕關(guān)節(jié)炎的診斷及其中醫(yī)證候量化分型提供了一種思路。
Abstract:
Objective:To explore the value of joint fluid metabolomics in diagnosis and COLD-HEAT SYMPTOM COMPLEX typing of rheumatoid arthritis(RA).Methods:The knee joint fluid of 12 patients with RA(COLD-DAMPNESS BLOCK COLLATERALS(寒濕阻絡(luò))in 6 patients and DAMPNESS-HEAT BLOCK COLLATERALS(濕熱阻絡(luò))in 6 patients)and 7 healthy volunteers were collected respectively and gas chromatography-mass spectrometry(GC-MS)was performed on the samples.The principal component analysis(PCA)was performed on GC-MS data of selected main chromatographic peak.Results:Twenty-three main chromatographic peaks were selected as common peaks for describing the chemical composition characteristics of joint fluid.The general characteristics of GC-MS total ion chromatogram of healthy volunteers was basically the same as that of RA patients.Twenty-three chromatographic peaks for describing the chemical composition characteristics of joint fluid were integrated into four principal components by using PCA.The PCA were performed on joint fluid samples of all the subjects.The scores of joint fluid samples of RA patients were characterized by concentrated distribution in the third and fourth principal component score plots,while those of healthy volunteers were characterized by relative dispersion.The PCA were performed only on joint fluid samples of RA patients,and the results showed that the scores of joint fluid samples were characterized by relative dispersion distribution in both patients with DAMPNESS-HEAT BLOCK COLLATERALS and patients with COLD-DAMPNESS BLOCK COLLATERALS in the third and fourth principal component score plots.Conclusion:The metabolome data of knee joint fluid can be used to accurately distinguish RA patients from healthy volunteers and distinguish DAMPNESS-HEAT BLOCK COLLATERALS from COLD-DAMPNESS BLOCK COLLATERALS,therefore it provides a new way for diagnosis and TCM symptom complex quantitative classification of RA.

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備注/Memo

備注/Memo:
2013-11-01收稿 2014-03-12修回
基金項(xiàng)目:浙江省中醫(yī)藥管理局項(xiàng)目(2007GA002)
通訊作者:童培建 E-mail:[email protected]
更新日期/Last Update: 2015-01-30