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[1]胡恩睿,魏義保,廖太陽,等.基于PPARγ/c-Ski信號通路探討蔓荊子黃素對膝骨關節(jié)炎大鼠滑膜組織纖維化的影響及其作用機制[J].中醫(yī)正骨,2025,37(04):11-19,29.
　HU Enrui,WEI Yibao,LIAO Taiyang,et al.Exploring the effects and mechanism of casticin on synovial fibrosis in knee osteoarthritis rats based on the PPARγ/c-Ski signaling pathway[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2025,37(04):11-19,29.
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基于PPARγ/c-Ski信號通路探討蔓荊子黃素對膝骨關節(jié)炎大鼠滑膜組織纖維化的影響及其作用機制()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第37卷
期數(shù):
2025年04期
頁碼:
11-19,29
欄目:
基礎研究
出版日期:
2025-04-20

文章信息/Info

Title:
Exploring the effects and mechanism of casticin on synovial fibrosis in knee osteoarthritis rats based on the PPARγ/c-Ski signaling pathway
作者:
胡恩睿; 魏義保; 廖太陽; 劉德仁; 龔子健; 王培民
南京中醫(yī)藥大學附屬醫(yī)院,江蘇 南京 210029
Author(s):
HU Enrui; WEI Yibao; LIAO Taiyang; LIU Deren; GONG Zijian; WANG Peimin
Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu,China
關鍵詞:
骨關節(jié)炎; 膝;  滑膜;  纖維化;  蔓荊子;  黃酮醇類;  炎癥;  過氧化物酶體增生物激活受體;  c-Ski;  信號傳導;  大鼠
Keywords:
osteoarthritis; knee;  synovial membrane;  fibrosis;  viticis fructus;  flavonols;  lnflammation;  peroxisome proliferator-activated receptors;  c-Ski;  signal transduction;  rats
摘要:
目的:探討蔓荊子黃素對膝骨關節(jié)炎(knee osteoarthritis,KOA)大鼠滑膜組織纖維化的影響及其作用機制。方法:將 40只成年雄性SD大鼠隨機分為假手術組、模型組、低濃度組、高濃度組和拮抗劑組,每組8只。模型組、低濃度組、高濃度組和拮抗劑組大鼠均采用前交叉韌帶離斷法建立右側KOA模型。造模14 d后,低濃度組和高濃度組大鼠分別按照0.2 mg·kg-1·d-1和0.4 mg·kg-1·d-1的劑量給予蔓荊子黃素灌胃; 拮抗劑組大鼠在給予蔓荊子黃素灌胃的同時,每日按照1.0 mg·kg-1的劑量腹腔注射GW9662溶液; 假手術組、模型組、低濃度組、高濃度組大鼠均每日腹腔注射等體積生理鹽水; 連續(xù)干預14 d。干預結束后,采用ELISA試劑盒檢測大鼠血清白細胞介素(interleukin,IL)-1β、腫瘤壞死因子-α(tumor necrosis factor-α,TNF-α)含量; 采用HE染色試劑盒、Masson染色試劑盒分別對大鼠膝關節(jié)滑膜組織進行染色,觀察大鼠滑膜炎癥程度與纖維化程度; 分別采用實時定量PCR和Western Blot法檢測大鼠膝關節(jié)滑膜組織中轉化生長因子(transforming growth factor,TGF)-β、α-平滑肌肌動蛋白(α-smooth muscle actin,α-SMA)、Ⅰ型膠原、過氧化物酶體增殖物激活受體(peroxisome proliferator-activated receptor,PPAR)γ、c-Ski的mRNA和蛋白表達水平。結果:①血清IL-1β和TNF-α含量檢測結果。模型組大鼠血清IL-1β、TNF-α含量均高于假手術組(P=0.000,P=0.000),低濃度組和高濃度組大鼠血清IL-1β、TNF-α含量均低于模型組(IL-1β:P=0.004,P=0.000; TNF-α:P=0.000,P=0.000),拮抗劑組大鼠血清IL-1β、TNF-α含量均高于高濃度組(P=0.027,P=0.005)。②膝關節(jié)滑膜組織病理學觀察結果。模型組大鼠膝關節(jié)滑膜組織部分缺損,襯里層細胞排列不規(guī)則,滑膜細胞大量增生,炎癥細胞浸潤明顯,膠原纖維大量沉積; 與模型組相比,低濃度組、高濃度組大鼠膝關節(jié)滑膜組織襯里層細胞排列較為規(guī)則,炎癥細胞浸潤明顯減輕,膠原纖維沉積減輕; 與高濃度組相比,拮抗劑組大鼠膝關節(jié)滑膜組織炎癥細胞浸潤更嚴重,膠原纖維沉積更多。③滑膜組織纖維化相關基因的mRNA和蛋白表達量檢測結果。模型組大鼠滑膜組織中TGF-β、α-SMA、Ⅰ型膠原的mRNA和蛋白相對表達量均高于假手術組(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),低濃度組和高濃度組大鼠滑膜組織中TGF-β、α-SMA、Ⅰ型膠原的mRNA和蛋白相對表達量均低于模型組(mRNA:P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000; 蛋白:P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),高濃度組大鼠滑膜組織中TGF-β、α-SMA、Ⅰ型膠原的mRNA和蛋白相對表達量均低于低濃度組(P=0.000,P=0.021,P=0.001; P=0.001,P=0.000,P=0.002),拮抗劑組大鼠滑膜組織中TGF-β、α-SMA、Ⅰ型膠原的mRNA和蛋白相對表達量均高于高濃度組(P=0.000,P=0.001,P=0.001; P=0.002,P=0.001,P=0.020)。④滑膜組織中PPARγ/c-Ski信號通路相關基因的mRNA和蛋白表達量檢測結果。模型組大鼠滑膜組織中PPARγ、c-Ski的mRNA和蛋白相對表達量均低于假手術組(P=0.000,P=0.000; P=0.000,P=0.000),低濃度組和高濃度組大鼠滑膜組織中PPARγ、c-Ski的mRNA和蛋白相對表達量均高于模型組(mRNA:P=0.001,P=0.000; P=0.004,P=0.000; 蛋白:P=0.001,P=0.000; P=0.004,P=0.000),高濃度組大鼠滑膜組織中PPARγ、c-Ski的mRNA和蛋白相對表達量均高于低濃度組(mRNA:P=0.000,P=0.000; 蛋白:P=0.049,P=0.000),拮抗劑組大鼠滑膜組織中PPARγ、c-Ski的mRNA和蛋白相對表達量均低于高濃度組(mRNA:P=0.000,P=0.000; 蛋白:P=0.000,P=0.000)。結論:蔓荊子黃素能夠改善KOA大鼠滑膜組織纖維化,且高濃度蔓荊子黃素比低濃度蔓荊子黃素的改善效果更佳; 其作用機制可能與其能夠激活PPARγ/c-Ski信號通路、抑制炎癥反應有關。
Abstract:
Objective:To observe the effects of casticin(CAS)on synovial fibrosis in knee osteoarthritis(KOA)rats,and to explore its underlying mechanism.Methods:Forty adult male SD rats were randomized into sham-operated group,model group,low-concentration group,high-concentration group,and antagonist group,with 8 ones in each group.All rats but the ones in sham-operated group were modeled by anterior cruciate ligment transection(ACLT)on the right knees for inducing KOA.Fourteen days after successful modeling,the rats in low- and high-concentration groups were intervened by intragastric administration with CAS at doses of 0.2 and 0.4 mg/kg/day,respectively; the ones in antagonist group firstly by intragastric administration with CAS,followed by intraperitoneal injection of GW9662 solution at a dose of 1.0 mg/kg; while the ones in sham-operated group,model group,low-concentration group,and high-concentration group by intraperitoneal injection of the same dose of normal saline every day for consecutive 14 days.After the end of intervention,the serum levels of interleukin(IL)-1β and tumor necrosis factor-α(TNF-α)were detected using ELISA kit.After that,the rat knee synovial tissues were harvested and stained with HE staining kit,and Masson staining kit,respectively,to observe the degree of synovial inflammation and fibrosis.Furthermore,the mRNA and protein expression levels of transforming growth factor(TGF)-β,α-smooth muscle actin(α-SMA),type I collagen,peroxisome proliferator-activated receptor(PPAR)γ,and c-Ski in the knee synovial tissues were detected by real-time quantitative PCR(RT-qPCR)and Western Blot,respectively.Results:①The serum levels of IL-1β and TNF-α.The serum levels of IL-1β and TNF-α were higher in model group compared to sham-operated group(P=0.000,P=0.000),were lower in low- and high-concentration groups compared to model group(IL-1β:P=0.004,P=0.000; TNF-α:P=0.000,P=0.000),and were higher in antagonist group compared to high-concentration group(P=0.027,P=0.005).②The pathological changes in knee synovial tissues.The changes,manifesting as partially defective knee synovial tissues,irregularly arranged lining layer cells,significantly proliferated synovial cells,substantially deposited collagen fibers,and obvious inflammatory cells infiltration,were observed in rats of model group.Compared with that of model group,the knee synovial tissues of rats in low- and high-concentration groups exhibited as more uniformly arranged lining layer cells,marked reduced inflammatory cell infiltration,and decreased collagen fiber deposition.While,compared with that of high-concentration group,the rats in antagonist group presented as more severe inflammatory cell infiltration and more increased collagen fiber deposition.③The mRNA and protein expression levels of fibrosis-related genes in the knee synovial tissues.The relative mRNA and protein expression levels of TGF-β,α-SMA,and type I collagen in knee the synovial tissues were higher in model group compared to sham-operated group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),were lower in low- and high-concentration groups compared to model group(mRNA:P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000; protein:P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),were lower in high-concentration group compared to low-concentration group(P=0.000,P=0.021,P=0.001; P=0.001,P=0.000,P=0.002),and were higher in antagonist group compared to high-concentration group(P=0.000,P=0.001,P=0.001; P=0.002,P=0.001,P=0.020).④The mRNA and protein expression levels of PPARγ/c-Ski signaling pathway-related genes in the knee synovial tissues.The relative mRNA and protein expression levels of PPARγ and c-Ski in the knee synovial tissues were lower in model group compared to sham-operated group(P=0.000,P=0.000; P=0.000,P=0.000),were higher in low- and high-concentration groups compared to model group(mRNA:P=0.001,P=0.000; P=0.004,P=0.000; protein:P=0.001,P=0.000; P=0.004,P=0.000),were higher in high-concentration group compared to low-concentration group(mRNA:P=0.000,P=0.000; protein:P=0.049,P=0.000),and were lower in an-tagonist group compared to high-concentration group(mRNA:P=0.000,P=0.000; protein:P=0.000,P=0.000).Conclusion:CAS can ameliorate the fibrosis of synovial tissues in KOA rats,and the high-concentration CAS behaves better compared to the low-concentration one.It may work by activating the PPARγ/c-Ski signaling pathway and inhibiting the inflammatory responses.

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備注/Memo

備注/Memo:
基金項目:國家自然科學基金(82274545); 江蘇省醫(yī)學重點學科和醫(yī)學重點實驗室建設項目(JSDW202252); 江蘇省中醫(yī)院臨床醫(yī)學創(chuàng)新中心發(fā)展項目(Y2023zx05)
通訊作者:王培民 E-mail:[email protected]
更新日期/Last Update: 1900-01-01