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[1]劉文潔,李輝,張玲玲,等.低頻電刺激股四頭肌和髖外展肌群治療膝骨關(guān)節(jié)炎的實驗研究[J].中醫(yī)正骨,2024,36(06):16-22，31.
　LIU Wenjie,LI Hui,ZHANG Lingling,et al.Low-frequency electrical stimulation of quadriceps femoris and hip abductor muscle group against knee osteoarthritis:an experimental study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2024,36(06):16-22，31.
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低頻電刺激股四頭肌和髖外展肌群治療膝骨關(guān)節(jié)炎的實驗研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第36卷
期數(shù):
2024年06期
頁碼:
16-22，31
欄目:
基礎(chǔ)研究
出版日期:
2024-06-20

文章信息/Info

Title:
Low-frequency electrical stimulation of quadriceps femoris and hip abductor muscle group against knee osteoarthritis:an experimental study
作者:
劉文潔; 李輝; 張玲玲; 陸永莉; 海博涵; 王文彪
新鄉(xiāng)醫(yī)學(xué)院第一附屬醫(yī)院,河南 衛(wèi)輝 453100
Author(s):
LIU Wenjie; LI Hui; ZHANG Lingling; LU Yongli; HAI Bohan; WANG Wenbiao
The First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan,China
關(guān)鍵詞:
骨關(guān)節(jié)炎; 膝;  電刺激;  股四頭肌;  髖外展肌群;  臀中肌;  大鼠;  白細胞介素IL-1β;  基質(zhì)金屬蛋白酶13;  動物實驗
Keywords:
osteoarthritis; knee;  electric stimulation;  quadriceps femoris;  hip abductor muscle group;  gluteus medius muscle;  rats;  interleukin-1beta;  matrix metalloproteinase 13;  animal experimentation
摘要:
目的:觀察低頻電刺激股四頭肌和髖外展肌群治療膝骨關(guān)節(jié)炎(knee osteoarthritis,KOA)的療效,并探討其可能的作用機制。方法:將40只2月齡SD大鼠隨機分為正常對照組、模型組、電刺激股四頭肌組、聯(lián)合電刺激股四頭肌組,每組10只。除正常對照組外,其余3組均分別于造模開始后第1天、第4天、第7天在大鼠右膝關(guān)節(jié)腔內(nèi)注射0.15 mL的4%木瓜蛋白酶溶液和濃度為0.03 mol·L-1的L-半胱氨酸溶液(2:1)的混合液。造模成功后,電刺激股四頭肌組和聯(lián)合電刺激股四頭肌組分別于大鼠右股四頭肌和股四頭肌與髖外展肌群(尤其是臀中肌)進行低頻電刺激,隔天干預(yù)1次,每次20 min,共28 d; 模型組和正常對照組不做任何干預(yù)。干預(yù)結(jié)束后第2天,抽取各組大鼠2 mL下腔靜脈血,采用酶聯(lián)免疫吸附法檢測大鼠血清中白細胞介素(interleukin,IL)-1β和基質(zhì)金屬蛋白酶(matrix metalloproteinase,MMP)-13的含量; 采血后處死大鼠,切取各組大鼠膝關(guān)節(jié)股骨遠端軟骨組織,分別進行蘇木精-伊紅(hematoxylin-eosin,HE)染色、甲苯胺藍染色和改良番紅O-固綠染色,并采用Mankin評分評估關(guān)節(jié)軟骨損傷情況; 采用免疫組織化學(xué)染色法檢測大鼠膝關(guān)節(jié)軟骨組織中MMP-13的表達量。結(jié)果:①大鼠膝關(guān)節(jié)軟骨組織病理學(xué)觀察結(jié)果。與正常對照組相比,模型組軟骨細胞排列紊亂、數(shù)量減少,軟骨基質(zhì)減少、染色明顯不均,軟骨層變薄,潮線模糊不清。電刺激股四頭肌組和聯(lián)合電刺激股四頭肌組軟骨細胞排列整齊、數(shù)目增多,軟骨基質(zhì)染色均一,軟骨層增厚,潮線清晰可見,其中聯(lián)合電刺激股四頭肌組表現(xiàn)更為明顯。②大鼠膝關(guān)節(jié)軟骨Mankin評分結(jié)果。正常對照組大鼠膝關(guān)節(jié)軟骨Mankin評分低于模型組、電刺激股四頭肌組、聯(lián)合電刺激股四頭肌組(P=0.000,P=0.000,P=0.000),電刺激股四頭肌組、聯(lián)合電刺激股四頭肌組大鼠膝關(guān)節(jié)軟骨Mankin評分低于模型組(P=0.000,P=0.000),聯(lián)合電刺激股四頭肌組大鼠膝關(guān)節(jié)軟骨Mankin評分低于電刺激股四頭肌組(P=0.000)。③大鼠血清中IL-1β和MMP-13含量檢測結(jié)果。正常對照組大鼠血清中IL-1β、MMP-13含量均低于模型組、電刺激股四頭肌組、聯(lián)合電刺激股四頭肌組(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),電刺激股四頭肌組、聯(lián)合電刺激股四頭肌組大鼠血清中IL-1β、MMP-13含量均低于模型組(P=0.000,P=0.000; P=0.000,P=0.000),聯(lián)合電刺激股四頭肌組血清中IL-1β、MMP-13含量均低于電刺激股四頭肌組(P=0.000,P=0.000)。④大鼠膝關(guān)節(jié)軟骨組織中MMP-13表達量檢測結(jié)果。正常對照組大鼠膝關(guān)節(jié)軟骨組織中MMP-13陽性細胞占比低于模型組、電刺激股四頭肌組和聯(lián)合電刺激股四頭肌組(P=0.000,P=0.000,P=0.000),電刺激股四頭肌組和聯(lián)合電刺激股四頭肌組大鼠膝關(guān)節(jié)軟骨組織中MMP-13陽性細胞占比均低于模型組(P=0.000,P=0.000),聯(lián)合電刺激股四頭肌組大鼠膝關(guān)節(jié)軟骨組織中MMP-13陽性細胞占比低于電刺激股四頭肌組(P=0.000)。結(jié)論:低頻電刺激股四頭肌和髖外展肌群能促進大鼠膝關(guān)節(jié)軟骨損傷的修復(fù),其作用機制可能與其能降低KOA大鼠血清中IL-1β、MMP-13的含量,抑制膝關(guān)節(jié)軟骨中MMP-13的表達有關(guān)。
Abstract:
Objective:To observe the outcomes of low-frequency electrical stimulation(LFES)of quadriceps femoris(QF)and hip abductor muscle group against knee osteoarthritis(KOA)in rats,and to explore its underlying mechanism.Methods:Forty 2-month-old Sprague-Dawley(SD)rats were randomized into normal control group,model group,QF LFES group and combined QF LFES group,10 ones ineach group.All rats but the ones in normal control group were intervened by knee intra-articular injection of a mixture of 4% papain solution and 0.03 mol/L L-cysteine solution in a ratio of 2:1(0.15 mL)into the right knees on day 1,4 and 7 after the beginning of the modeling for inducing KOA.After successful modeling,the rats in QF LFES group and combined QF LFES group received LFES on the right QF as well as QF and hip abductor muscle group(especially the gluteus medius),respectively,once every other day,20 minutes at a time for consecutive 28 days; while the ones in model group and normal control group were not given any intervention.On day 2 after the end of intervention,the blood(2 mL)was drawn from the inferior vena cava(IVC)of rats in each group,and the serum levels of interleukin(IL)-1β and matrix metalloproteinase(MMP)-13 were detected by using enzyme linked immunosorbent assay(ELISA).After phlebotomizing,the rats were sacrificed,and their distal femoral cartilage tissues were harvested and stained with hematoxylin-eosin(HE),toluidine blue and modified safranin O-fast green(SO-FG)for observing the histopathological changes; meanwhile,the knee articular cartilage injury was evaluated by using Mankin score.Furthermore,the expression level of MMP-13 in the knee cartilage tissues were detected by using immunohistochemical staining.Results:①Compared with that of normal control group,the histopathological changes,manifesting as decreased and disordered arranged chondrocytes,reduced cartilage matrix with obviously uneven staining,thinned cartilage layer,and the unclear tidal line,were observed in the knee cartilage tissues of rats in model group; while,the rats in QF LFES group and combined QF LFES group presented with increased and neatly arranged chondrocytes,uniformly stained cartilage matrix,thickened cartilage layer and clear tidal lines,with combined QF LFES group performed more pronounced.②The Mankin score of the knee cartilage was higher in model group,QF LFES group and combined QF LFES group compared to normal control group(P=0.000,P=0.000,P=0.000),and was higher in model group and QF LFES group compared to combined QF LFES group(P=0.000,P=0.000),and was highest in model group(P=0.000).③The serum levels of IL-1β and MMP-13 were higher in model group,QF LFES group and combined QF LFES group compared to normal control group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),and was higher in model group and QF LFES group compared to combined QF LFES group(P=0.000,P=0.000; P=0.000,P=0.000),and was highest in model group(P=0.000,P=0.000).④The MMP-13-positive cells accounted for a higher proportion in the knee cartilage tissues of rats in model group,QF LFES group and combined QF LFES group compared to normal control group(P=0.000,P=0.000,P=0.000),and a higher proportion in model group and QF LFES group compared to combined QF LFES group(P=0.000,P=0.000),and a highest proportion in model group(P=0.000).Conclusion:LFES of QF and hip abductor muscle group can promote the repair of knee cartilage injury in KOA rats.It may exert the effects by reducing the serum levels of IL-1β and MMP-13 and inhibiting the expression of MMP-13 in knee cartilage of KOA rats.

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備注/Memo

備注/Memo:
基金項目:河南省醫(yī)學(xué)科技攻關(guān)項目(LHGJ20190464)
通訊作者:王文彪 E-mail:[email protected]
更新日期/Last Update: 1900-01-01