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[1]廖太陽(yáng),馬振源,康俊峰,等.基于“柔肝養(yǎng)筋”理論探討小鼠肝臟來源外泌體對(duì)膝骨關(guān)節(jié)炎模型小鼠膝關(guān)節(jié)軟骨的影響[J].中醫(yī)正骨,2024,36(03):7-14,22.
　LIAO Taiyang,MA Zhenyuan,KANG Junfeng,et al.Effects of hepatocyte-derived exosomes from mice on knee cartilage of model mice with knee osteoarthritis:a softening liver and nourishing tendons theory-based experimental study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2024,36(03):7-14,22.
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基于“柔肝養(yǎng)筋”理論探討小鼠肝臟來源外泌體對(duì)膝骨關(guān)節(jié)炎模型小鼠膝關(guān)節(jié)軟骨的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第36卷
期數(shù):
2024年03期
頁(yè)碼:
7-14,22
欄目:
基礎(chǔ)研究
出版日期:
2024-03-20

文章信息/Info

Title:
Effects of hepatocyte-derived exosomes from mice on knee cartilage of model mice with knee osteoarthritis:a softening liver and nourishing tendons theory-based experimental study
作者:
廖太陽(yáng); 馬振源; 康俊峰; 楊楠; 王培民; 邢潤(rùn)麟
江蘇省中醫(yī)院,江蘇 南京 210029
Author(s):
LIAO Taiyang; MA Zhenyuan; KANG Junfeng; YANG Nan; WANG Peimin; XING Runlin
Jiangsu Province Hospital of Chinese Medicine,Nanjing 210029,Jiangsu,China
關(guān)鍵詞:
外泌體;  軟骨;  骨關(guān)節(jié)炎; 膝;  小鼠;  動(dòng)物實(shí)驗(yàn)
Keywords:
exosomes;  cartilage;  osteoarthritis; knee;  mice;  animal experimentation
摘要:
目的:基于“柔肝養(yǎng)筋”理論探討小鼠肝臟來源外泌體對(duì)膝骨關(guān)節(jié)炎(knee osteoarthritis,KOA)模型小鼠膝關(guān)節(jié)軟骨的影響。方法:以超速離心法從3月齡(年輕)和22月齡(老年)C57BL/6雄性小鼠肝臟提取外泌體。將48只7周齡SPF級(jí)C57BL/6J雄性小鼠隨機(jī)分為4組,每組12只。KOA組、Young-EVs組及Aged-EVs組小鼠采用前交叉韌帶橫斷術(shù)進(jìn)行KOA造模,Sham組小鼠接受手術(shù)但不離斷前交叉韌帶。術(shù)后14 d開始,向Young-EVs組和Aged-EVs組小鼠膝關(guān)節(jié)腔分別注射10 μL年輕和老年小鼠肝臟來源外泌體,每周2次,連續(xù)注射4周; Sham組和KOA組用同樣方法向膝關(guān)節(jié)腔注射10 μL PBS溶液。外泌體干預(yù)結(jié)束后,對(duì)各組小鼠造模側(cè)膝關(guān)節(jié)進(jìn)行X線檢查; 蘇木精-伊紅染色和番紅O-固綠染色后進(jìn)行組織病理學(xué)觀察,并采用國(guó)際骨關(guān)節(jié)炎研究協(xié)會(huì)(Osteoarthritis Research Society International,OARSI)骨關(guān)節(jié)炎軟骨組織病理學(xué)評(píng)價(jià)系統(tǒng)及Mankin病理評(píng)分系統(tǒng)評(píng)價(jià)小鼠軟骨退變程度; 免疫組化法檢測(cè)軟骨組織中Ⅱ型膠原蛋白(collagenⅡ,COLⅡ)、聚集蛋白聚糖(aggrecan,ACAN)、基質(zhì)金屬蛋白酶(matrix metalloproteinase,MMP)13和一種具有血小板反應(yīng)蛋白基序的去整合素和金屬蛋白酶(a disintegrin and metalloproteinase with thrombospondin motifs,ADAMTS)5表達(dá)量。結(jié)果:①小鼠膝關(guān)節(jié)X線檢查結(jié)果。Sham組小鼠膝關(guān)節(jié)關(guān)節(jié)間隙正常,關(guān)節(jié)面平整光滑,軟骨完整,關(guān)節(jié)邊緣無骨贅形成,Kellgren-Lawrence分級(jí)為0級(jí); KOA組小鼠膝關(guān)節(jié)間隙未見明顯狹窄,關(guān)節(jié)面粗糙,軟骨出現(xiàn)部分缺損,關(guān)節(jié)邊緣可見明顯骨贅形成,Kellgren-Lawrence分級(jí)為Ⅱ級(jí); Young-EVs組小鼠膝關(guān)節(jié)間隙輕微變窄,關(guān)節(jié)表面稍有粗糙表現(xiàn),軟骨缺損較KOA組減少,關(guān)節(jié)邊緣可見輕微骨贅形成,Kellgren-Lawrence分級(jí)為Ⅰ級(jí); Aged-EVs組小鼠膝關(guān)節(jié)間隙、關(guān)節(jié)表面粗糙程度、軟骨缺損情況、關(guān)節(jié)內(nèi)游離體數(shù)量較KOA組更為嚴(yán)重,Kellgren-Lawrence分級(jí)為Ⅲ級(jí)。②小鼠膝關(guān)節(jié)軟骨組織病理學(xué)觀察結(jié)果。4組小鼠膝關(guān)節(jié)軟骨OARSI評(píng)分和Mankin評(píng)分組間總體比較,差異均有統(tǒng)計(jì)學(xué)意義[OARSI評(píng)分:(0.417±0.376)分,(4.500±0.632)分,(2.417±0.492)分,(5.417±0.492)分,F=116.800,P=0.000; Mankin評(píng)分:(1.083±0.665)分,(7.250±0.880)分,(3.583±0.917)分,(8.833±0.683)分,F=117.100,P=0.000]。KOA組、Young-EVs組及Aged-EVs組的OARSI評(píng)分和Mankin評(píng)分均高于Sham組(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000); Young-EVs組的OARSI評(píng)分和Mankin評(píng)分均低于KOA組(P=0.000,P=0.000); Aged-EVs組的OARSI評(píng)分和Mankin評(píng)分均高于KOA組、Young-EVs組(P=0.025,P=0.000; P=0.015,P=0.000)。③小鼠膝關(guān)節(jié)軟骨組織免疫組織化學(xué)檢測(cè)結(jié)果。4組小鼠膝關(guān)節(jié)軟骨中COLⅡ和ACAN陽(yáng)性表達(dá)面積比組間總體比較,差異均有統(tǒng)計(jì)學(xué)意義[COLⅡ:(40.050±2.324)%,(26.083±1.119)%,(23.317±2.599)%,(16.717±2.479)%,F=118.500,P=0.000; ACAN:(51.500±3.191)%,(17.700±3.415)%,(19.383±3.924)%,(12.300±1.908)%,F=185.500,P=0.000]。KOA組、Young-EVs組及Aged-EVs組的COLⅡ和ACAN陽(yáng)性表達(dá)面積比均低于Sham組(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000); Young-EVs組與KOA組COLⅡ、ACAN陽(yáng)性表達(dá)面積比的組間差異均無統(tǒng)計(jì)學(xué)意義(P=0.167,P=0.799); Aged-EVs組的COLⅡ和ACAN陽(yáng)性表達(dá)面積比均低于KOA組、Young-EVs組(P=0.000,P=0.000; P=0.039,P=0.005)。4組MMP13和ADAMTS5陽(yáng)性表達(dá)面積比組間總體比較,差異均有統(tǒng)計(jì)學(xué)意義[MMP13:(11.733±2.191)%,(46.150±6.237)%,(34.417±5.027)%,(57.233±4.049)%,F=106.600,P=0.000; ADAMTS5:(7.950±1.344)%,(30.533±6.184)%,(22.083±3.418)%,(39.317±5.606)%,F=51.450,P=0.000]。KOA組、Young-EVs組及Aged-EVs組的MMP13和ADAMTS5陽(yáng)性表達(dá)面積比均高于Sham組(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000); Young-EVs組的MMP13和ADAMTS5陽(yáng)性表達(dá)面積比均低于KOA組(P=0.002,P=0.021); Aged-EVs組的MMP13和ADAMTS5陽(yáng)性表達(dá)面積比均高于KOA組、Young-EVs組(P=0.003,P=0.000; P=0.016,P=0.000)。結(jié)論:小鼠肝臟來源外泌體可能參與軟骨細(xì)胞合成及分解代謝反應(yīng); 其中老年小鼠肝臟來源外泌體可加速細(xì)胞外基質(zhì)降解、減少聚集蛋白聚糖合成,加劇KOA小鼠軟骨退變; 而年輕小鼠肝臟來源外泌體可抑制軟骨細(xì)胞外基質(zhì)降解,保護(hù)KOA小鼠軟骨。
Abstract:
Objective:To explore the effects of hepatocyte-derived exosomes(HDEs)from mice on knee cartilage of model mice with knee osteoarthritis(KOA)based on softening liver and nourishing tendons theory.Methods:The exosomes were isolated and extracted from the livers of young(3-month-old)and aged(22-month-old)male C57BL/6 mice by using ultracentrifugation.Forty-eight 7-week-old specific pathogen free(SPF)-grade male C57BL/6J mice were randomized into KOA group,Young-EVs group,Aged-EVs group and Sham group,12 ones in each group.The mice in KOA group,Young-EVs group and Aged-EVs group were subjected to anterior cruciate ligament(ACL)transection for inducing KOA; while the ones in Sham group only underwent surgery,but without transecting ACL.At postoperative day 14 after the modeling,the mice in Young-EVs group and Aged-EVs group were intervened by knee intra-articular injection of 10 μL HDEs extracted from young and aged mice,respectively,two times a week for consecutive 4 weeks; while the ones in Sham group and KOA group with 10 μL phosphate buffered saline(PBS)solution.After the end of intervention,the knee joints of the modeling side in mice of each group were checked by X-ray scanning,and were evaluated by Kellgren-Lawrence(K-L)grading system.After the end of X-ray examination,the knees were harvested from mice of each group,and the knee tissue sections were stained with hematoxylin-eosin(HE)and safranin O-fast green(SO-FG)for observing the histopathological changes; meanwhile,the degree of knee articular cartilage degeneration was evaluated by using Osteoarthritis Research Society International(OARSI)osteoarthritis cartilage histopathology assessment system and Mankin scoring system.Furthermore,the expression levels of collagenⅡ(COLⅡ),aggrecan(ACAN),matrix metalloproteinase(MMP)13 and a disintegrin and metalloproteinase with thrombospondin motifs(ADAMTS)5 in knee cartilage tissues were detected by using immunohistochemistry(IHC).Results:①The results of X-ray examination showed that the mice in Sham group presented with normal knee joint space,smooth articular surface,and intact cartilage,and no osteophyte was observed at the margin of knee joint.The K-L classification belonged to grade 0.The changes,manifesting as rough articular surface,partially defective cartilage and definite osteophytes at the margin of knee joint,were observed,while,the marked knee joint space narrowing(JSN)were unobserved in mice of KOA group.The K-L classification belonged to gradeⅡ.The slight JSN,slightly rough articular surface,defective cartilage(decreased compared to KOA group),and small osteophytes at the margin of knee joint were observed in mice of Young-EVs group.The K-L classification belonged to gradeⅠ.Compared to KOA group,the changes,including knee joint space,articular surface roughness,cartilage defects,and the number of free bodies within the joint,were more severe in mice of Aged-EVs group.The K-L classification belonged to gradeⅢ.②There was statistical difference in OARSI score and Mankin score between the 4 groups(OARSI score:0.417±0.376,4.500±0.632,2.417±0.492,5.417±0.492 points,F=116.800,P=0.000; Mankin score:1.083±0.665,7.250±0.880,3.583±0.917,8.833±0.683 points,F=117.100,P=0.000).The OARSI score and Mankin score were higher in KOA group,Young-EVs group and Aged-EVs group compared to Sham group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),and were higher in KOA group compared to Young-EVs group(P=0.000,P=0.000),and were highest in Aged-EVs group(P=0.025,P=0.000; P=0.015,P=0.000).③There was statistical difference in the positive expression area ratios of COLⅡand ACAN in knee cartilage between the 4 groups(COLⅡ:40.050±2.324,26.083±1.119,23.317±2.599,16.717±2.479%,F=118.500,P=0.000; ACAN:51.500±3.191,17.700±3.415,19.383±3.924,12.300±1.908%,F=185.500,P=0.000).The positive expression area ratios of COLⅡand ACAN were lower in KOA group,Young-EVs group and Aged-EVs group compared to Sham group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),and were lower in Aged-EVs group compared to KOA group and Young-EVs group(P=0.000,P=0.000; P=0.039,P=0.005),while,the comparison between Young-EVs group and KOA group revealed no significant differences(P=0.167,P=0.799).There was statistical difference in the positive expression area ratios of MMP13 and ADAMTS5 in knee cartilage between the 4 groups(MMP13:11.733±2.191,46.150±6.237,34.417±5.027,57.233±4.049%,F=106.600,P=0.000; ADAMTS5:7.950±1.344,30.533±6.184,22.083±3.418,39.317±5.606%,F=51.450,P=0.000).The positive expression area ratios of MMP13 and ADAMTS5 were higher in KOA group,Young-EVs group and Aged-EVs group compared to Sham group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),and were higher in KOA group compared to Young-EVs group(P=0.002,P=0.021),and were highest in Aged-EVs group(P=0.003,P=0.000; P=0.016,P=0.000).Conclusion:The mice HDEs may be involved in the synthesis and catabolic reactions of chondrocytes,among which HDEs from the aged mice can accelerate the degradation of extracellular matrix and reduce the synthesis of ACAN,thereby exacerbating the cartilage degeneration in KOA mice; whereas the ones from the young mice can inhibit the degradation of cartilage extracellular matrix,thus exerting the effect of protecting cartilage in KOA mice.

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備注/Memo

備注/Memo:
基金項(xiàng)目:國(guó)家自然科學(xué)基金項(xiàng)目(81804123); 江蘇省第六期“333人才”培養(yǎng)資助項(xiàng)目(第三層次青年人才項(xiàng)目); 江蘇省中醫(yī)藥科技發(fā)展計(jì)劃青年人才項(xiàng)目(QN202210); 南京中醫(yī)藥大學(xué)自然科學(xué)基金項(xiàng)目(XZR2020005); 江蘇省中醫(yī)院高峰學(xué)術(shù)人才項(xiàng)目(Y2021rc39); 江蘇省醫(yī)學(xué)重點(diǎn)學(xué)科和醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室建設(shè)項(xiàng)目(JSDW202252)
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更新日期/Last Update: 1900-01-01