84年鼠女哪年财运最旺,857comvvv色九欧美激情|85PO_87国产精品欲av国产av资源

[1]馬力,汪盛玉,韓軍燁,等.長(zhǎng)鏈非編碼RNA HAGLR與微RNA-19a-3p對(duì)股骨頸骨折內(nèi)固定術(shù)后骨折不愈合的影響[J].中醫(yī)正骨,2023,35(04):1-6.
　MA Li,WANG Shengyu,HAN Junye,et al.Effects of long non-coding RNA HAGLR and microRNA-19a-3p on bone nonunion after internal fixation of femoral neck fractures[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2023,35(04):1-6.
點(diǎn)擊復(fù)制

長(zhǎng)鏈非編碼RNA HAGLR與微RNA-19a-3p對(duì)股骨頸骨折內(nèi)固定術(shù)后骨折不愈合的影響()
分享到：

《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第35卷
期數(shù):
2023年04期
頁(yè)碼:
1-6
欄目:
臨床研究
出版日期:
2023-04-20

文章信息/Info

Title:
Effects of long non-coding RNA HAGLR and microRNA-19a-3p on bone nonunion after internal fixation of femoral neck fractures
作者:
馬力; 汪盛玉; 韓軍燁; 付豪
(松滋市人民醫(yī)院,湖北 松滋 434200)
Author(s):
MA Li; WANG Shengyu; HAN Junye; FU Hao
Songzi People's Hospital,Songzi 434200,Hubei,China
關(guān)鍵詞:
股骨頸骨折;  骨折固定術(shù); 內(nèi);  骨折; 不愈合;  RNA; 長(zhǎng)鏈非編碼;  微RNAs
Keywords:
femoral neck fractures;  fracture fixation; internal;  fractures; ununited;  RNA; long noncoding;  microRNAs
摘要:
目的:觀察長(zhǎng)鏈非編碼RNA(long non-coding RNA,lncRNA)HAGLR與微RNA(microRNA,miRNA)-19a-3p對(duì)股骨頸骨折內(nèi)固定術(shù)后骨折不愈合的影響。方法:回顧性分析采用切開(kāi)復(fù)位AO中空加壓螺釘內(nèi)固定治療的股骨頸骨折患者的病例資料,包括性別、年齡、基礎(chǔ)疾病(高血壓、糖尿病、冠心病)、損傷類型、骨折類型、骨折愈合情況,以及采用實(shí)時(shí)定量PCR檢測(cè)法測(cè)得的外周血液中l(wèi)ncRNA HAGLR和miRNA-19a-3p表達(dá)量。按照術(shù)后骨折愈合情況分為愈合組和不愈合組。先對(duì)2組患者的相關(guān)信息進(jìn)行單因素分析,再采用Logistic回歸分析研究骨折不愈合的影響因素,最后采用受試者操作特征(receiver operating characteristic,ROC)曲線分析評(píng)價(jià)外周血液中l(wèi)ncRNA HAGLR與miRNA-19a-3p表達(dá)量對(duì)骨折不愈合的預(yù)測(cè)價(jià)值。結(jié)果:①骨折不愈合影響因素的單因素分析結(jié)果。共納入141例患者,男73例、女68例。愈合組120例,不愈合組21例。2組患者年齡、損傷類型、骨折類型的比較,組間差異均有統(tǒng)計(jì)學(xué)意義(t=3.395,P=0.001; χ2=16.904,P=0.000; χ2=4.727,P=0.030)。術(shù)后4周、8周、12周,愈合組外周血液中l(wèi)ncRNA HAGLR表達(dá)量均高于不愈合組(0.79±0.20,0.63±0.15,t=3.494,P=0.001; 0.87±0.17,0.75±0.16,t=3.009,P=0.003; 0.95±0.16,0.87±0.19,t=2.054,P=0.042),miRNA-19a-3p表達(dá)量均低于不愈合組(1.19±0.29,1.40±0.30,t=3.046,P=0.001; 1.13±0.25,1.27±0.27,t=2.340,P=0.003; 1.03±0.22,1.18±0.20,t=2.919,P=0.042)。②骨折不愈合影響因素的多因素分析結(jié)果。將損傷類型、骨折類型以及術(shù)后4周外周血液中l(wèi)ncRNA HAGLR和miRNA-19a-3p表達(dá)量作為自變量,將骨折是否愈合作為因變量,進(jìn)行Logistic回歸分析,結(jié)果顯示高能量損傷、骨折移位、術(shù)后4周外周血液中miRNA-19a-3p表達(dá)量是股骨頸骨折內(nèi)固定術(shù)后骨折不愈合的危險(xiǎn)因素(β=2.582,P=0.005,OR=1.423; β=1.734,P=0.011,OR=1.272; β=136.44,P=0.001,OR=23.698),術(shù)后4周外周血液中l(wèi)ncRNA HAGLR表達(dá)量是股骨頸骨折內(nèi)固定術(shù)后骨折不愈合的保護(hù)因素(β=-109.00,P=0.001,OR=0.000)。③lncRNA HAGLR和miRNA-19a-3p表達(dá)量對(duì)骨折不愈合的預(yù)測(cè)結(jié)果。ROC曲線分析結(jié)果顯示,采用術(shù)后4周外周血液中l(wèi)ncRNA HAGLR和miRNA-19a-3p表達(dá)量預(yù)測(cè)骨折不愈合的靈敏度分別為61.67%、81.67%,特異度分別為80.95%、47.62%,曲線下面積分別為0.710(P=0.002)、0.673(P=0.012)。結(jié)論:股骨頸骨折內(nèi)固定術(shù)后4周時(shí)外周血液中l(wèi)ncRNA HAGLR表達(dá)量下降、miRNA-19a-3p表達(dá)量升高提示骨折不愈合風(fēng)險(xiǎn)增加,這兩項(xiàng)指標(biāo)可用作預(yù)測(cè)股骨頸骨折內(nèi)固定術(shù)后骨折不愈合的生物標(biāo)志物。
Abstract:
Objective:To observe the effects of long non-coding RNA(lncRNA)HAGLR and microRNA(miRNA)-19a-3p on bone nonunion after internal fixation of femoral neck fractures.Methods:The medical data of patients with femoral neck fractures treated with open reduction and internal fixation using AO hollow compression screws were retrospectively analyzed,including gender,age,underlying diseases(hypertension,diabetes,and coronary heart disease),injury type,fracture type,and fracture healing outcome.Real-time quantitative PCR was used to detect the expression levels of lncRNA HAGLR and miRNA-19a-3p in the peripheral blood.The patients were divided into a union group and a nonunion group according to the postoperative fracture healing outcomes.Firstly,univariate analysis was performed on the relevant information of patients in the two groups,followed by Logistic regression analysis for the influencing factors of bone nonunion.Finally,the receiver operating characteristic(ROC)curve was used to analyze and evaluate the predictive value of the expression levels of lncRNA HAGLR and miRNA-19a-3p in the peripheral blood for bone nonunion.Results:①Univariate analysis results of factors affecting bone nonunion.A total of 141 patients were involved,including 73 males and 68 females.There were 120 patients in the union group and 21 patients in the nonunion group.There were statistically significant differences in age,injury type,and fracture type between the two groups(t=3.395,P=0.001; χ2=16.904,P=0.000; χ2=4.727,P=0.030).At 4,8,and 12 weeks after surgery,the expression levels of lncRNA HAGLR in the peripheral blood of the union group were higher than those in the nonunion group(0.79±0.20 vs 0.63±0.15,t=3.494,P=0.001; 0.87±0.17 vs 0.75±0.16,t=3.009,P=0.003; 0.95±0.16 vs 0.87±0.19,t=2.054,P=0.042),while the expression levels of miRNA-19a-3p in the union group were lower than those in the nonunion group(1.19±0.29 vs 1.40±0.30,t=3.046,P=0.001; 1.13±0.25 vs 1.27±0.27,t=2.340,P=0.003; 1.03±0.22 vs 1.18±0.20,t=2.919,P=0.042).②Multivariate analysis results of factors affecting bone nonunion.Logistic regression analysis was carried out with injury type,fracture type,and expression levels of lncRNA HAGLR and miRNA-19a-3p in the peripheral blood at 4 weeks after surgery as independent variables and bone union/nonunion as the dependent variable.The results showed that high-energy injury,fracture displacement,and expression level of miRNA-19a-3p in the peripheral blood at 4 weeks after surgery were risk factors for bone nonunion of femoral neck fractures treated with internal fixation(β=2.582,P=0.005,OR=1.423; β=1.734,P=0.011,OR=1.272; β=136.44,P=0.001,OR=23.698),while the expression level of lncRNA HAGLR in the peripheral blood at 4 weeks after surgery was the protective factor for bone nonunion of femoral neck fractures treated with internal fixation(β=-109.00,P=0.001,OR=0.000).③Predictive results of lncRNA HAGLR and miRNA-19a-3p expre-ssion levels for bone nonunion of fractures.The results of ROC curve analysis showed that the sensitivity for predicting bone nonunion of fractures using lncRNA HAGLR and miRNA-19a-3p expression levels in the peripheral blood at 4 weeks after surgery was 61.67% and 81.67%,respectively,with specificities of 80.95% and 47.62%.The areas under curve were 0.710(P=0.002)and 0.673(P=0.012),respectively.Conclusion:The decreased expression of lncRNA HAGLR and increased expression of miRNA-19a-3p in the peripheral blood at 4 weeks after internal fixation of femoral neck fractures suggest an increased risk of bone nonunion.These two indicators can be used as biological markers to predict bone nonunion after internal fixation of femoral neck fractures.

參考文獻(xiàn)/References:

[1] REAHL G B,GERSTENFELD L,KAIN M.Epidemiology,clinical assessments,and current treatments of nonunions[J].Curr Osteoporos Rep,2020,18(3):157-168.
[2] 裴福興,陳安民.骨科學(xué)[M].北京:人民衛(wèi)生出版社,2016:256-261.
[3] 滕延斌,李亭亭,王磊,等.股骨頸骨折內(nèi)固定術(shù)后骨折不愈合或畸形愈合的危險(xiǎn)因素分析[J].中華創(chuàng)傷骨科雜志,2020,22(9):799-803.
[4] BOURGERY M,EKHOLM E,FAGERLUND K,et al.Multiple targets identified with genome wide profiling of small RNA and mRNA expression are linked to fracture healing in mice[J/OL].Bone Rep,2021,15:101115[2022-01-26].https://pubmed.ncbi.nlm.nih.gov/34458508/.
[5] SILVA A M,MOURA S R,TEIXEIRA J H,et al.Long noncoding RNAs:a missing link in osteoporosis[J].Bone Res,2019,7:10.
[6] PAN L X,DING W.LncRNA HAGLR accelerates femoral neck fracture healing through negatively regulating miRNA-19a-3p[J].Eur Rev Med Pharmacol Sci,2020,24(8):4080-4087.
[7] 中華醫(yī)學(xué)會(huì)骨科學(xué)分會(huì)創(chuàng)傷骨科學(xué)組,中國(guó)醫(yī)師協(xié)會(huì)骨科醫(yī)師分會(huì)創(chuàng)傷專家工作委員會(huì).成人股骨頸骨折診治指南[J].中華創(chuàng)傷骨科雜志,2018,20(11):921-928.
[8] 趙勇,秦偉凱.重視股骨頸骨折的評(píng)估與內(nèi)固定治療的若干問(wèn)題[J].中國(guó)骨傷,2021,34(3):195-199.
[9] 邵永科,施松青,袁耀.股骨頸骨折后股骨頭后傾對(duì)內(nèi)固定術(shù)后再手術(shù)率的影響[J].現(xiàn)代實(shí)用醫(yī)學(xué),2018,30(7):922-924.
[10] XIA Y,ZHANG W,ZHANG Z,et al.Treatment of femoral neck fractures:sliding hip screw or cannulated screws?a meta-analysis[J].J Orthop Surg Res,2021,16(1):54.
[11] TANG Y,ZHANG Z,WANG L,et al.Femoral neck system versus inverted cannulated cancellous screw for the treatment of femoral neck fractures in adults:a preliminary comparative study[J].J Orthop Surg Res,2021,16(1):504.
[12] SANTOSHI J A,REDDY L,AGRAWAL U.Femoral neck nonunion associated with delayed union of ipsilateral femoral shaft fracture[J].Cureus,2021,13(6):e15612.
[13] 楊曉強(qiáng),薛繼釗,鄭程,等.青壯年股骨頸骨折的內(nèi)固定治療進(jìn)展[J].中醫(yī)正骨,2021,33(7):38-44.
[14] RAMADANOV N,TOMA I,HERKNER H,et al.Factors that influence the complications and outcomes of femoral neck fractures treated by cannulated screw fixation[J].Sci Rep,2020,10(1):758.
[15] 李少帥,張宏軍.PauwelsⅢ型股骨頸骨折內(nèi)固定方式的選擇及常見(jiàn)問(wèn)題探討[J].中醫(yī)正骨,2018,30(11):57-59.
[16] RATHWA H S,VERMA T,CHAVALI V H.Assessment of union in fractures:role of serum alkaline phosphatase and ultrasonography[J].J Clin Orthop Trauma,2020,14:94-100.
[17] WU J,LIU L,HU H,et al.Bioinformatic analysis and experimental identification of blood biomarkers for chronic nonunion[J].J Orthop Surg Res,2020,15(1):208.
[18] ZHU J,HU H,DENG X,et al.Nomogram for predicting reoperation following internal fixation of nondisplaced femoral neck fractures in elderly patients[J].J Orthop Surg Res,2021,16(1):544.
[19] 李江華,李新梅,陳磊,等.血清骨膜素水平與老年髖部骨折患者骨密度相關(guān)性研究[J].中國(guó)骨質(zhì)疏松雜志,2020,26(1):59-63.
[20] 張文韜,段寧,陳勛,等.血清血管細(xì)胞黏附因子-1、骨形態(tài)發(fā)生蛋白-2動(dòng)態(tài)監(jiān)測(cè)對(duì)尺橈骨骨折延遲愈合的預(yù)測(cè)價(jià)值[J].中國(guó)臨床醫(yī)生雜志,2019,47(2):198-200.
[21] ZHANG Y,ZHANG Y,YANG K,et al.MALAT1 knockdown promoted cell viability and migration of LPS-treated MG-63 cells via sponging miR-212[J].Genes Genomics,2021,43(5):523-531.
[22] 郭瑋瑋,秦悅,楊海波,等. LncRNA MALAT1通過(guò)miR-34c/SATB2軸對(duì)脂肪間充質(zhì)干細(xì)胞成骨分化的促進(jìn)作用[J].吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版),2020,46(5):963-971.
[23] LI G,YUN X,YE K,et al.Long non-coding RNA-H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA-149/SDF-1 axis[J].J Cell Mol Med,2020,24(9):4944-4955.
[24] 李峰,劉翠中,伍媛.LncRNA-H19通過(guò)抑制miR-185-5p的表達(dá)促進(jìn)成骨細(xì)胞的增殖[J].湖南師范大學(xué)學(xué)報(bào)(醫(yī)學(xué)版),2021,18(4):13-17.
[25] DAI Z Z,ZHANG Z Q,DING J,et al.Analysis of risk factors for complications after femoral neck fracture in pediatric patients[J].J Orthop Surg Res,2020,15(1):58.
(收稿日期:2022-01-27 本文編輯:郭毅曼)

相似文獻(xiàn)/References:

[1]溫超海,何忠,李永斌,等.閉合復(fù)位伽瑪釘改良成鎖定支架內(nèi)固定治療 老年股骨轉(zhuǎn)子間骨折[J].中醫(yī)正骨,2015,27(11):32.
[2]肖善富,張喜善,于鳳珍.髕下小切口鋼絲紐扣式內(nèi)固定治療脛骨髁間隆突骨折[J].中醫(yī)正骨,2015,27(11):34.
[3]朱峰,宋相建,吳蔚,等.手法復(fù)位經(jīng)皮克氏針內(nèi)固定治療 兒童閉合性肱骨遠(yuǎn)端全骨骺分離[J].中醫(yī)正骨,2015,27(11):51.
[4]裘人華,胡培陽(yáng),裘天強(qiáng),等.閉合復(fù)位外固定架固定治療Colles骨折[J].中醫(yī)正骨,2015,27(11):59.
[5]葛波涌,王玉波,王明太,等.交鎖髓內(nèi)釘內(nèi)固定治療脛骨干骨折的臨床研究[J].中醫(yī)正骨,2015,27(10):8.
　GE Boyong,WANG Yubo,WANG Mingtai,et al.Clinical study on the internal fixation with interlocking intramedullary nail for the treatment of tibial shaft fractures[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):8.
[6]鄧紅敏,劉志誠(chéng).轉(zhuǎn)子間斜行截骨結(jié)合股骨近端防旋髓內(nèi)釘固定治療 股骨近端骨折術(shù)后髖內(nèi)翻畸形[J].中醫(yī)正骨,2015,27(10):17.
[7]孫群周,阮成群,李光明,等.股骨近端防旋髓內(nèi)釘內(nèi)固定治療 老年不穩(wěn)定型股骨轉(zhuǎn)子間骨折[J].中醫(yī)正骨,2015,27(10):20.
[8]方策,倪新宇,王宏偉,等.遲氏正骨手法復(fù)位單臂外固定支架固定治療 老年股骨轉(zhuǎn)子間骨折[J].中醫(yī)正骨,2015,27(10):22.
[9]全先輝,萬(wàn)春友,劉磊,等.Taylor空間支架外固定治療脛腓骨開(kāi)放性骨折[J].中醫(yī)正骨,2015,27(10):30.
[10]茆軍,張惠法,嚴(yán)培軍,等.Ilizarov外固定支架結(jié)合克氏針固定治療Pilon骨折[J].中醫(yī)正骨,2015,27(10):32.
[11]劉震,胡志國(guó),付偉.抖牽旋按復(fù)位結(jié)合鋼針撬撥復(fù)位空心釘內(nèi)固定治療 難復(fù)性股骨頸骨折[J].中醫(yī)正骨,2015,27(08):28.
[12]林統(tǒng),徐強(qiáng),包海燕.閉合復(fù)位經(jīng)皮空心加壓螺釘內(nèi)固定聯(lián)合口服中藥 治療股骨頸骨折[J].中醫(yī)正骨,2015,27(07):61.
[13]陳武林,阮成群.縫匠肌肌蒂髂骨骨瓣移植聯(lián)合股骨近端外側(cè)鎖定鋼板固定治療青壯年股骨頸骨折不愈合[J].中醫(yī)正骨,2017,29(02):41.
[14]嚴(yán)衛(wèi)鋒,曾忠友,裴斐.髖關(guān)節(jié)直接前方入路切開(kāi)復(fù)位空心加壓螺釘內(nèi)固定治療難復(fù)位性股骨頸骨折[J].中醫(yī)正骨,2017,29(09):45.
[15]王斌,鐘浩博,楊劍鋒.3種內(nèi)固定治療PauwelsⅢ型股骨頸骨折的生物力學(xué)研究[J].中醫(yī)正骨,2018,30(07):6.
　WANG Bin,ZHONG Haobo,YANG Jianfeng.A biomechanical study of three kinds of internal fixation for treatment of Pauwels typeⅢfemoral neck fractures[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2018,30(04):6.
[16]李少帥,張宏軍.PauwelsⅢ型股骨頸骨折內(nèi)固定方式的選擇及常見(jiàn)問(wèn)題探討[J].中醫(yī)正骨,2018,30(11):57.
[17]王淼鑫,袁西岐,鄭作超,等.股骨近端鎖定鈦板內(nèi)固定治療股骨頸骨折[J].中醫(yī)正骨,2018,30(11):68.
[18]張忠良,林俊宏,費(fèi)霖莉.代謝綜合征對(duì)股骨頸骨折空心螺釘內(nèi)固定術(shù)預(yù)后的影響[J].中醫(yī)正骨,2020,32(04):21.
　ZHANG Zhongliang,LIN Junhong,FEI Linli.Effects of metabolic syndrome on prognosis of patients who received surgery of hollow screw internal fixation for femoral neck fractures[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2020,32(04):21.
[19]范克杰,陳柯,田可為,等.手法閉合復(fù)位聯(lián)合平行螺釘和偏軸螺釘內(nèi)固定治療青壯年垂直不穩(wěn)定型股骨頸骨折[J].中醫(yī)正骨,2020,32(07):47.
[20]丁強(qiáng),劉玉峰,蘇藝,等.克氏針與空心螺釘內(nèi)固定治療兒童DelbetⅡ、Ⅲ型股骨頸骨折[J].中醫(yī)正骨,2020,32(11):69.

更新日期/Last Update: 1900-01-01