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[1]王最,徐佳妮,吳良邦,等.骨關(guān)節(jié)炎軟骨損傷中miRNA-214的作用機(jī)制及靶向基因研究[J].中醫(yī)正骨,2023,35(03):6-14,30.
　WANG Zui,XU Jiani,WU Liangbang,et al.Mechanism and targeted genes of miRNA-214 in osteoarthritis-induced cartilage injury[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2023,35(03):6-14,30.
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骨關(guān)節(jié)炎軟骨損傷中miRNA-214的作用機(jī)制及靶向基因研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第35卷
期數(shù):
2023年03期
頁(yè)碼:
6-14,30
欄目:
基礎(chǔ)研究
出版日期:
2023-03-20

文章信息/Info

Title:
Mechanism and targeted genes of miRNA-214 in osteoarthritis-induced cartilage injury
作者:
王最1; 徐佳妮1; 吳良邦1; 錢(qián)鈞2
(1.解放軍聯(lián)勤保障部隊(duì)第九〇三醫(yī)院,浙江 杭州 310004; 2.衢州市人民醫(yī)院,浙江 衢州 324000)
Author(s):
WANG Zui1; XU Jiani1; WU Liangbang1; QIAN Jun2
1.The 903rd Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army,Hangzhou 310004,Zhejiang,China 2.Quzhou People's Hospital,Quzhou 324000,Zhejiang,China
關(guān)鍵詞:
骨關(guān)節(jié)炎;  軟骨疾病;  微RNAs;  基因表達(dá);  大鼠;  動(dòng)物實(shí)驗(yàn)
Keywords:
osteoarthritis;  cartilage diseases;  microRNAs;  gene expression;  rats;  animal experimentation
摘要:
目的:探討骨關(guān)節(jié)炎(osteoarthritis,OA)軟骨損傷中微小RNA(micro RNA,miRNA)-214的作用機(jī)制及靶向基因。方法:①OA患者膝關(guān)節(jié)損傷軟骨組織中miRNA-214表達(dá)量檢測(cè)。根據(jù)軟骨損傷程度Outerbridge分級(jí)標(biāo)準(zhǔn)將收集的OA患者膝關(guān)節(jié)軟骨組織進(jìn)行分級(jí),分別提取各軟骨組織的總RNA,逆轉(zhuǎn)錄cDNA后采用實(shí)時(shí)定量PCR檢測(cè)損傷軟骨組織中miRNA-214的表達(dá)量。②miRNA-214在OA大鼠軟骨損傷中的作用機(jī)制分析。采用手術(shù)剪斷大鼠前交叉韌帶的方法建立大鼠OA模型。將40只造模成功的大鼠隨機(jī)分為miRNA-214高表達(dá)組、miRNA-214低表達(dá)組、陰性對(duì)照組及模型組,將10只接受手術(shù)但不剪斷前交叉韌帶的大鼠納入假手術(shù)組。設(shè)計(jì)、合成miRNA-214模擬物、miRNA-214抑制劑及miRNA-214陰性對(duì)照序列,構(gòu)建慢病毒表達(dá)載體,完成病毒包裝。在miRNA-214高表達(dá)組、miRNA-214低表達(dá)組、陰性對(duì)照組、模型組及假手術(shù)組大鼠右側(cè)膝關(guān)節(jié)腔內(nèi)分別注射100 μL miRNA-214模擬物慢病毒混懸液、100 μL miRNA-214抑制劑慢病毒混懸液、100 μL miRNA-214陰性對(duì)照慢病毒混懸液、100 μL生理鹽水、100 μL生理鹽水。干預(yù)后4周,采集各組大鼠腹主動(dòng)脈血,檢測(cè)血清白細(xì)胞介素(interleukin,IL)-17、IL-23水平; 制備各組大鼠膝關(guān)節(jié)軟骨組織石蠟切片,HE染色后觀察軟骨組織病理學(xué)改變; 采用免疫印跡法檢測(cè)各組大鼠膝關(guān)節(jié)軟骨組織中聚集蛋白聚糖、Ⅱ型膠原蛋白(collagenⅡ,ColⅡ)、基質(zhì)金屬蛋白酶(matrix metalloproteinase,MMP)-13的蛋白相對(duì)表達(dá)量。③OA軟骨損傷相關(guān)的miRNA-214靶向基因分析。檢索miRNA靶基因數(shù)據(jù)庫(kù)中miRNA-214的靶向基因,根據(jù)文獻(xiàn)資料篩選與骨代謝相關(guān)的人類(lèi)miRNA-214靶向基因。采用雙熒光素酶實(shí)驗(yàn)驗(yàn)證miRNA-214對(duì)活化轉(zhuǎn)錄因子4(activating transcription factor 4,ATF4)的靶向性。采用免疫印跡法檢測(cè)各組大鼠右側(cè)膝關(guān)節(jié)軟骨組織中ATF4的蛋白相對(duì)表達(dá)量。結(jié)果:①OA患者膝關(guān)節(jié)損傷軟骨組織中miRNA-214表達(dá)量檢測(cè)結(jié)果。共收集38例OA患者的膝關(guān)節(jié)軟骨組織,其中Ⅲ級(jí)28例、Ⅳ級(jí)10例。Ⅳ級(jí)損傷軟骨組織的miRNA-214相對(duì)表達(dá)量高于Ⅲ級(jí)(1.67±0.40,0.51±0.16,t=12.941,P=0.000)。②大鼠血清炎癥因子水平檢測(cè)結(jié)果。5組大鼠血清IL-17、IL-23水平比較,組間差異有統(tǒng)計(jì)學(xué)意義[(62.94±8.12)pg·mL-1,(45.19±5.34)pg·mL-1,(44.63±6.67)pg·mL-1,(24.74±3.90)pg·mL-1,(18.15±2.33)pg·mL-1,F=94.153,P=0.000;(72.39±10.26)pg·mL-1,(48.70±5.87)pg·mL-1,(47.59±5.76)pg·mL-1,(27.54±4.05)pg·mL-1,(18.13±3.05)pg·mL-1,F=111.701,P=0.000]。miRNA-214 高表達(dá)組、陰性對(duì)照組、模型組及miRNA-214低表達(dá)組大鼠血清IL-17、IL-23水平均高于假手術(shù)組(P=0.000,P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000,P=0.000); miRNA-214高表達(dá)組大鼠血清IL-17、IL-23水平均高于陰性對(duì)照組、模型組及miRNA-214低表達(dá)組(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000); miRNA-214低表達(dá)組大鼠血清IL-17、IL-23水平均低于陰性對(duì)照組和模型組(P=0.000,P=0.000; P=0.000,P=0.000); 陰性對(duì)照組大鼠血清IL-17、IL-23水平與模型組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.838,P=0.675)。③大鼠軟骨組織病理學(xué)檢查結(jié)果。HE染色結(jié)果顯示,假手術(shù)組軟骨組織潮線(xiàn)清晰,結(jié)構(gòu)完整,軟骨細(xì)胞分布均勻; 陰性對(duì)照組、模型組軟骨組織潮線(xiàn)模糊不清,結(jié)構(gòu)嚴(yán)重破壞,軟骨細(xì)胞聚集; miRNA-214高表達(dá)組軟骨組織損傷情況較陰性對(duì)照組、模型組更為嚴(yán)重; miRNA-214低表達(dá)組軟骨組織潮線(xiàn)可辨識(shí),結(jié)構(gòu)較為完整,軟骨細(xì)胞聚集現(xiàn)象較陰性對(duì)照組、模型組及miRNA-214高表達(dá)組有所改善。④大鼠軟骨組織聚集蛋白聚糖、ColⅡ、MMP-13蛋白表達(dá)量檢測(cè)結(jié)果。5組大鼠軟骨組織聚集蛋白聚糖、ColⅡ、MMP-13蛋白表達(dá)量比較,組間差異均有統(tǒng)計(jì)學(xué)意義(0.08±0.02,0.14±0.03,0.15±0.04,0.31±0.08,0.72±0.12,F=142.932,P=0.000; 0.11±0.03,0.20±0.04,0.22±0.04,0.36±0.05,1.13±0.21,F=170.345,P=0.000; 1.32±0.34,0.95±0.13,0.95±0.12,0.21±0.04,0.11±0.03,F=91.486,P=0.000)。miRNA-214高表達(dá)組、陰性對(duì)照組、模型組及miRNA-214低表達(dá)組大鼠軟骨組織聚集蛋白聚糖、ColⅡ蛋白表達(dá)量均低于假手術(shù)組(P=0.000,P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000,P=0.000),MMP-13蛋白表達(dá)量高于假手術(shù)組(P=0.000,P=0.000,P=0.000,P=0.000); miRNA-214高表達(dá)組大鼠軟骨組織聚集蛋白聚糖、ColⅡ蛋白表達(dá)量均低于陰性對(duì)照組、模型組及miRNA-214低表達(dá)組(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),MMP-13蛋白表達(dá)量高于陰性對(duì)照組、模型組及miRNA-214低表達(dá)組(P=0.005,P=0.004,P=0.000); miRNA-214低表達(dá)組大鼠軟骨組織聚集蛋白聚糖、ColⅡ蛋白表達(dá)量均高于陰性對(duì)照組和模型組(P=0.000,P=0.000; P=0.000,P=0.000),MMP-13蛋白表達(dá)量低于陰性對(duì)照組和模型組(P=0.000,P=0.000); 陰性對(duì)照組大鼠軟骨組織聚集蛋白聚糖、ColⅡ、MMP-13蛋白表達(dá)量與模型組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.535,P=0.278,P=1.000)。⑤miRNA-214靶向基因驗(yàn)證結(jié)果。在轉(zhuǎn)染ATF4-WT-psiCHECK2質(zhì)粒的293T細(xì)胞中,miRNA-214模擬物組的熒光酶相對(duì)活性小于miRNA-214陰性對(duì)照組(0.45±0.07,1.02±0.23,t=5.301,P=0.001); 在轉(zhuǎn)染ATF4-MUT-psiCHECK2質(zhì)粒的293T細(xì)胞中,miRNA-214模擬物組的熒光酶相對(duì)活性與miRNA-214陰性對(duì)照組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(1.05±0.19,1.03±0.18,t=0.171,P=0.869)。⑥大鼠軟骨組織中ATF4蛋白表達(dá)量檢測(cè)結(jié)果。5組大鼠軟骨組織ATF4蛋白表達(dá)量比較,組間差異有統(tǒng)計(jì)學(xué)意義(0.11±0.03,0.17±0.03,0.18±0.04,0.31±0.05,0.79±0.11,F=213.111,P=0.000)。miRNA-214高表達(dá)組、陰性對(duì)照組、模型組及miRNA-214低表達(dá)組大鼠軟骨組織ATF4蛋白表達(dá)量均低于假手術(shù)組(P=0.000,P=0.000,P=0.000,P=0.000); miRNA-214高表達(dá)組大鼠軟骨組織ATF4蛋白表達(dá)量低于陰性對(duì)照組、模型組及miRNA-214低表達(dá)組(P=0.000,P=0.000,P=0.000); miRNA-214低表達(dá)組大鼠軟骨組織ATF4蛋白表達(dá)量高于陰性對(duì)照組和模型組(P=0.000,P=0.000); 陰性對(duì)照組大鼠軟骨組織ATF4蛋白表達(dá)量與模型組比較,組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.535)。結(jié)論:OA患者膝關(guān)節(jié)軟骨損傷與軟骨組織中miRNA-214的表達(dá)有關(guān),抑制miRNA-214表達(dá)能夠減輕大鼠膝關(guān)節(jié)炎癥反應(yīng)、減少軟骨基質(zhì)降解、促進(jìn)軟骨修復(fù),miRNA-214的作用機(jī)制與其抑制IL-17、IL-23、MMP-13表達(dá)和促進(jìn)聚集蛋白聚糖、ColⅡ、ATF4表達(dá)有關(guān),ATF4可能是與OA軟骨損傷相關(guān)的miRNA-214的靶基因之一。
Abstract:
Objective:To investigate the mechanism and targeted genes of micro RNA(miRNA)-214 in osteoarthritis(OA)-induced cartilage injury.Methods:①Detection of miRNA-214 expression in injured cartilage of the knee joint in patients with OA.The knee cartilage tissues of OA patients were graded according to the Outerbridge classification for cartilage injury.The total RNA of each cartilage tissue was extracted,and the expression of miRNA-214 in the injured cartilage was detected by real-time quantitative PCR after reverse transcription of cDNA.②Analysis of mechanism of miRNA-214 in cartilage injury in OA rats.The OA model was established in rats by the surgical cutting of the anterior cruciate ligament.Forty OA model rats were randomly divided into a miRNA-214 high expression group,a miRNA-214 low expression group,a negative control group,and a model group.Another 10 rats undergoing surgery without cutting the anterior cruciate ligament were assigned into the sham operation group.The sequences of miRNA-214 mimics,miRNA-214 inhibitors,and miRNA-214 negative control were designed and synthesized,and lentivirus expression vector was constructed,followed by lentiviral packaging.Rats in the miRNA-214 high expression group,the miRNA-214 low expression group,the negative control group,the model group,and the sham operation group were injected with 100 μL of miRNA-214 mimic lentivirus suspension,100 μL of miRNA-214 inhibitor lentivirus suspension,100 μL of miRNA-214 negative control lentivirus suspension,100 μL of normal saline,and 100 μL of normal saline,respectively,at the right knee cavity.At four weeks after the intervention,blood in the abdominal aorta of all rats was sampled and serum interleukin(IL)-17 and IL-23 levels were measured.Paraffin sections of knee cartilage tissues were prepared,and the pathological changes in cartilage tissues were observed following HE staining.The relative protein expression levels of aggrecan,collagenⅡ(ColⅡ),and matrix metalloproteinase(MMP)-13 in the knee cartilage tissues of rats in each group were determined by Western blot.③Analysis of miRNA-214 targeted genes associated with OA-induced cartilage injury.The targeted genes of miRNA-214 were retrieved from the miRNA targeted gene database,and the human miRNA-214 targeted genes related to bone metabolism were screened according to the literature.The dual luciferase assay was employed to verify the targeting of miRNA-214 on activating transcription factor 4(ATF4).The relative protein expression of ATF4 in the right knee cartilage tissues of rats in each group was detected by Western blot.Results:①Detection results of miRNA-214 expression in injured cartilage tissues of knee joint in OA patients.Thirty-eight patients with OA were enrolled,including 28 with gradeⅢand 10 with gradeⅣ.The relative expression level of miRNA-214 in gradeⅣinjured cartilage tissues was higher than that in gradeⅢinjured cartilage tissues(1.67±0.40 vs 0.51±0.16,t=12.941,P=0.000).②Detection results of serum inflammatory factors in rats.The serum levels of IL-17 and IL-23 in the five groups were statistically significant(62.94±8.12,45.19±5.34,44.63±6.67,24.74±3.90,18.15±2.33 pg/mL,F=94.153,P=0.000; 72.39±10.26,48.70±5.87,47.59±5.76,27.54±4.05,18.13±3.05 pg/mL,F=111.701,P=0.000).Serum levels of IL-17 and IL-23 in the miRNA-214 high expression group,the negative control group,the model group,and the miRNA-214 low expression group were higher than those in the sham operation group(P=0.000,P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000,P=0.000).Serum levels of IL-17 and IL-23 in the miRNA-214 high expression group were higher than those in the negative control group,the model group,and the miRNA-214 low expression group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000).Serum levels of IL-17 and IL-23 in the miRNA-214 low expression group were lower than those in the negative control group and the model group(P=0.000,P=0.000; P=0.000,P=0.000).There was no significant difference in serum levels of IL-17 and IL-23 between the negative control group and the model group(P=0.838,P=0.675).③Pathological examination results of cartilage tissues.As revealed by HE staining,the sham operation group showed clear tide mark of cartilage tissues with intact structure and uniform distribution of chondrocytes,and the negative control group and the model group displayed blurred tide mark of cartilage tissues with seriously damaged structure and aggregated chondrocytes.The cartilage injury in the miRNA-214 high expression group was severer than that in the negative control group and the model group.The miRNA-214 low expression group exhibited distinguishable tide mark of cartilage tissues with comparatively intact structure,and chondrocyte aggregation was improved compared with the conditions in the negative control group,the model group,and the miRNA-214 high expression group.④Detection results of protein expression levels of aggrecan,ColⅡ,and MMP-13 in rat cartilage tissues.There were significant differences in protein expression levels of aggrecan,ColⅡ,and MMP-13 in cartilage tissues of rats between the five groups(0.08±0.02,0.14±0.03,0.15±0.04,0.31±0.08,0.72±0.12,F=142.932,P=0.000; 0.11±0.03,0.20±0.04,0.22±0.04,0.36±0.05,1.13±0.21,F=170.345,P=0.000; 1.32±0.34,0.95±0.13,0.95±0.12,0.21±0.04,0.11±0.03,F=91.486,P=0.000).The protein expression levels of aggrecan and ColⅡin the miRNA-214 high expression group,the negative control group,the model group,and the miRNA-214 low expression group were lower than those in the sham operation group(P=0.000,P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000,P=0.000),and the protein expression levels of MMP-13 were higher than that in the sham operation group(P=0.000,P=0.000,P=0.000,P=0.000).The protein expression levels of aggrecan and ColⅡin the miRNA-214 high expression group were lower than those in the negative control group,the model group,and the miRNA-214 low expression group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000),and the protein expression level of MMP-13 was higher than that in the negative control group,the model group,and the miRNA-214 low expression group(P=0.005,P=0.004,P=0.000).The protein expression levels of aggrecan and ColⅡin the miRNA-214 low expression group were higher than those in the negative control group and the model group(P=0.000,P=0.000; P=0.000,P=0.000),and the protein expression level of MMP-13 was lower than that in the negative control group and the model group(P=0.000,P=0.000).There was no significant difference in the protein expression levels of aggrecan,ColⅡ,and MMP-13 in cartilage tissues of rats between the negative control group and the model group(P=0.535,P=0.278,P=1.000).⑤Results of miRNA-214 targeted gene verification.In 293T cells transfected with ATF4-WT-psiCHECK2 plasmid,the relative activity of luciferase in the miRNA-214 mimics group was lower than that in the miRNA-214 negative control group(0.45±0.07 vs 1.02±0.23,t=5.301,P=0.001).In 293T cells transfected with ATF4-MUT-psiCHECK2 plasmid,there was no significant difference in the relative activity of luciferase between the miRNA-214 mimics group and the miRNA-214 negative control group(1.05±0.19 vs 1.03±0.18,t=0.171,P=0.869).⑥D(zhuǎn)etection results of ATF4 protein expression in rat cartilage tissues.The protein expression of ATF4 in the cartilage tissues of rats in the five groups was statistically significant(0.11±0.03,0.17±0.03,0.18±0.04,0.31±0.05,0.79±0.11,F=213.111,P=0.000).The protein expression level of ATF4 in cartilage tissues of rats in the miRNA-214 high expression group,the negative control group,the model group,and the miRNA-214 low expression group were lower than that in the sham operation group(P=0.000,P=0.000,P=0.000,P=0.000).The protein expression level of ATF4 in cartilage tissues of rats in the miRNA-214 high expression group was lower than that in the negative control group,the model group,and the miRNA-214 low expression group(P=0.000,P=0.000,P=0.000).The protein expression level of ATF4 in cartilage tissues of rats in the miRNA-214 low expression group was higher than that in the negative control group and the model group(P=0.000,P=0.000).There was no significant difference in ATF4 protein expression between the negative control group and the model group(P=0.535).Conclusion:Cartilage injury of knee joint in OA patients is related to the expression of miRNA-214 in cartilage tissues,and the inhibition of miRNA-214 expression can relieve knee inflammatory response,reduce cartilage stromal breakdown,and promote cartilage repair in rats.The underlying mechanism of miRNA-214 is attributed to the inhibition of the expression of IL-17,IL-23,and MMP-13 and the promotion of the expression of aggrecan,ColⅡ,and ATF4.ATF4 is potentially one of the targeted genes of miRNA-214 associated with OA-induced cartilage injury.

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更新日期/Last Update: 1900-01-01