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[1]王檸,何育風(fēng),揭宇予,等.補陽還五湯治療腰椎間盤突出癥作用機制的網(wǎng)絡(luò)藥理學(xué)研究[J].中醫(yī)正骨,2022,34(04):4-11.
　WANG Ning,HE Yufeng,JIE Yuyu,et al.Mechanism of action of Buyang Huanwu Tang(補陽還五湯)for treatment of lumbar disc herniation:a network pharmacology study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2022,34(04):4-11.
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補陽還五湯治療腰椎間盤突出癥作用機制的網(wǎng)絡(luò)藥理學(xué)研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第34卷
期數(shù):
2022年04期
頁碼:
4-11
欄目:
基礎(chǔ)研究
出版日期:
2022-04-20

文章信息/Info

Title:
Mechanism of action of Buyang Huanwu Tang(補陽還五湯)for treatment of lumbar disc herniation:a network pharmacology study
作者:
王檸; 何育風(fēng); 揭宇予; 左高駢; 張龍; 易樹堅
(廣西中醫(yī)藥大學(xué)第一附屬醫(yī)院,廣西 南寧 530023)
Author(s):
WANG Ning; HE Yufeng; JIE Yuyu; ZUO Gaopian; ZHANG Long; YI Shujian
The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China
關(guān)鍵詞:
椎間盤移位;  腰椎;  補陽還五湯;  網(wǎng)絡(luò)藥理學(xué);  藥物作用機制
Keywords:
intervertebral disc displacement;  lumbar vertebrae;  Buyang Huanwu Tang;  network pharmacology;  mechanism of drug action
摘要:
目的:采用網(wǎng)絡(luò)藥理學(xué)方法探討補陽還五湯治療腰椎間盤突出癥(lumbar disc herniation,LDH)的作用機制。方法:通過檢索中藥系統(tǒng)藥理學(xué)數(shù)據(jù)庫與分析平臺(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)和文獻(xiàn)篩選補陽還五湯的活性成分和靶點。利用在線人類孟德爾遺傳(online Mendelian inheritance in man,OMIM)數(shù)據(jù)庫、GeneCards數(shù)據(jù)庫、Disgenet數(shù)據(jù)庫檢索LDH的靶點,通過與補陽還五湯的靶點取交集,獲取補陽還五湯治療LDH的靶點。利用Cytoscape3.8.0軟件構(gòu)建補陽還五湯治療LDH的“活性成分-靶點”網(wǎng)絡(luò)及靶點蛋白互作網(wǎng)絡(luò),篩選補陽還五湯治療LDH的主要活性成分和關(guān)鍵靶點,并進(jìn)行分子對接。同時利用R軟件進(jìn)行靶點基因GO分析和KEGG分析。結(jié)果:從TCMSP和文獻(xiàn)中共篩選到補陽還五湯的活性成分47個、靶點265個。從OMIM數(shù)據(jù)庫、GeneCard數(shù)據(jù)庫、Disgenet數(shù)據(jù)庫共檢索到LDH的靶點392個。將補陽還五湯靶點和LDH靶點取交集后得到補陽還五湯治療LDH的靶點46個。補陽還五湯治療LDH的“活性成分-靶點”網(wǎng)絡(luò)和靶點蛋白互作網(wǎng)絡(luò)顯示,槲皮素、木犀草素、山柰酚及黃芩素為補陽還五湯治療LDH的主要活性成分,JUN原癌基因(JUN proto-oncogene,JUN)、腫瘤壞死因子(tumor necrosis factor,TNF)、AKT絲氨酸/蘇氨酸激酶 1(AKT serine/threonine kinase 1,AKT1)、絲裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)、腫瘤蛋白P53(tumor protein P53,TP53)、基質(zhì)金屬蛋白酶9(matrix metalloproteinase 9,MMP9)為補陽還五湯治療LDH的關(guān)鍵靶點。補陽還五湯治療LDH的靶點基因GO分析和KEGG分析結(jié)果顯示,補陽還五湯治療LDH的分子生物學(xué)機制主要涉及晚期糖基化終末產(chǎn)物(advanced glycation end product,AGE)-晚期糖基化終末產(chǎn)物受體(advanced glycation end product receptor,RAGE)信號通路、白細(xì)胞介素17(interleukin 17,IL-17)信號通路、TNF信號通路,參與對營養(yǎng)水平、氧化應(yīng)激、脂多糖以及細(xì)菌起源分子的反應(yīng)等生物過程。分子對接結(jié)果顯示,4個主要活性成分(槲皮素、木犀草素、山柰酚、黃芩素)與6個關(guān)鍵靶點(JUN、TNF、AKT1、MAPK1、TP53、MMP9)均具有良好的結(jié)合能力。結(jié)論:補陽還五湯可能是通過調(diào)控AGE-RAGE、IL-17及TNF等信號通路,減輕機體氧化應(yīng)激反應(yīng)、抑制炎癥因子表達(dá),發(fā)揮對LDH的治療作用; 槲皮素、木犀草素、山柰酚及黃芩素可能是補陽還五湯治療LDH的主要活性成分,JUN、TNF、AKT1、MAPK1、MMP9、TP53是對應(yīng)的關(guān)鍵靶點。
Abstract:
Objective:To explore the mechanism of action of Buyang Huanwu Tang(補陽還五湯,BYHWT)in treatment of lumbar disc herniation(LDH)by using network pharmacology approach.Methods:The active ingredients and action targets of BYHWT were screened by retrieving traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)and articles.The action targets of LDH were searched out from online Mendelian inheritance in man(OMIM)database,GeneCards database and Disgenet database.The targets of BYHWT for treatment of LDH were obtained through overlapping the targets of LDH with BYHWT.The active ingredient-target network and protein-protein interaction(PPI)network of BYHWT for treatment of LDH were built by using Cytoscape3.8.0 software for screening the main active ingredients and key targets of BYHWT for treatment of LDH,and the molecular docking was conducted.The GO function and KEGG pathway enrichment analysis were performed on key target genes respectively by using R software to dig biological functions and pathways of the key targets.Results:Forty-seven active ingredients and 265 targets of BYHWT were screened out from TCMSP and articles,and 392 targets of LDH were obtained from OMIM database,GeneCards database and Disgenet database.Forty-six targets of BYHWT for treatment of LDH were obtained through overlapping the targets of BYHWT with LDH.The active ingredient-target network diagram and PPI network diagram of BYHWT for treatment of LDH illustrated that quercetin,luteolin,kaempferol and baicalein were the main active ingredients of BYHWT for treatment of LDH,and JUN proto-oncogene(JUN),tumor necrosis factor(TNF),AKT serine/threonine kinase 1(AKT1),mitogen-activated protein kinase 1(MAPK1),tumor protein P53(TP53)and matrix metalloproteinase 9(MMP9)were the key targets of BYHWT for treatment of LDH.The results of KEGG signaling pathway enrichment analysis suggested that the molecular biological mechanism of BYHWT for treatment of LDH mainly related to advanced glycation end product(AGE)-advanced glycation end product receptor(RAGE)signaling pathway,interleukin 17(IL-17)signaling pathway and TNF signaling pathway.The results of GO function enrichment analysis indicated that the biological processes of BYHWT for treatment of LDH were mainly involved in the responses to nutrient level,oxidative stress,lipopolysaccharides and bacteria-derived molecules.The results of molecular docking showed that all of the 4 main active ingredients(quercetin,luteolin,kaempferol and baicalein)could bind well to the 6 key targets(JUN,TNF,AKT1,MAPK1,TP53 and MMP9).Conclusion:BYHWT may reduce oxidative stress response and inhibit the expression of inflammatory factors through regulating AGE-RAGE,IL-17 and TNF signaling pathways for treatment of LDH.Quercetin,luteolin,kaempferol and baicalein may be the main active ingredients and JUN,TNF,AKT1,MAPK1,MMP9 and TP53 are the corresponding key targets of BYHWT in treatment of LDH.

參考文獻(xiàn)/References:

[1] 田偉.實用骨科學(xué)[M].2版.北京:人民衛(wèi)生出版社,2016:636.
[2] 戚晴雪,劉華,甄朋超,等.夏氏六步手法對血瘀氣滯型腰椎間盤突出癥患者下肢神經(jīng)傳導(dǎo)的影響[J].中國中醫(yī)骨傷科雜志,2020,28(4):24-28.
[3] 胡均毅.補陽還伍湯加味治療腰椎間盤突出癥120例[J].中國中西醫(yī)結(jié)合外科雜志,1996,2(5):60-61.
[4] LIPINSKI C A.Rule of five in 2015 and beyond:target and ligand structural limitations,ligand chemistry structure and drug discovery project decisions[J].Adv Drug Deliv Rev,2016,101:34-41.
[5] BENZAKOUR T,IGOUMENOU V,MAVROGENIS A F,et al.Current concepts for lumbar disc herniation[J].Int Orthop,2019,43(4):841-851.
[6] 趙雪巍,劉培玉,劉丹,等.黃酮類化合物的構(gòu)效關(guān)系研究進(jìn)展[J].中草藥,2015,46(21):3264-3271.
[7] ROTELLI A E,AGUILAR C F,PELZER L E.Structural basis of the anti-inflammatory activity of quercetin:inhibition of the 5-hydroxytryptamine type 2 receptor[J].Eur Biophys J,2009,38(7):865-871.
[8] AZIZ N,KIM M Y,CHO J Y.Anti-inflammatory effects of luteolin:a review of in vitro,in vivo,and in silico studies[J].J Ethnopharmacol,2018,225:342-358.
[9] WONG S K,CHIN K Y,IMA-NIRWANA S.The osteoprotective effects of kaempferol:the evidence from in vivo and in vitro studies[J].Drug Des Devel Ther,2019,13:3497-3514.
[10] YANG S,WANG H,YANG Y,et al.Baicalein administered in the subacute phase ameliorates ischemia-reperfusion-induced brain injury by reducing neuroinflammation and neuronal damage[J].Biomed Pharmacother,2019,117:109.
[11] JOHNSON Z I,SCHOEPFLIN Z R,CHOI H,et al.Disc in flames:roles of TNF-α and IL-1β in intervertebral disc degeneration[J].Eur Cell Mater,2015,30:104-116.
[12] LE MAITRE C L,POCKERT A,BUTTLE D J,et al.Matrix synthesis and degradation in human intervertebral disc degeneration[J].Biochem Soc Trans,2007,35(Pt 4):652-655.
[13] LU X Y,DING X H,ZHONG L J,et al.Expression and significance of VEGF and p53 in degenerate intervertebral disc tissue[J].Asian Pac J Trop Med,2013,6(1):79-81.
[14] KAY A M,SIMPSON C L,STEWART J A Jr.The role of AGE/RAGE signaling in diabetes-mediated vascular calcification[J/OL].J Diabetes Res,2016[2021-12-01]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980539.
[15] DUBOIS C,FRADIN C,BOULANGER é.Axe AGE-RAGE:conséquences physiopathologiques et inflam-maging[J].Médecine des Maladies Métaboliques,2019,13(7):595-601.
[16] JIANG H,DENG Y,WANG T,et al.Interleukin-23 may contribute to the pathogenesis of lumbar disc herniation through the IL-23/IL-17 pathway[J].J Orthop Surg Res,2016,11:12.

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備注/Memo

備注/Memo:
基金項目:國家自然科學(xué)基金項目(82160943)通訊作者:何育風(fēng) E-mail:[email protected]
更新日期/Last Update: 1900-01-01