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[1]樓鵬強(qiáng),鄧小磊,魏波,等.2種冷凍時長進(jìn)行液氮冷凍法犬股骨頭壞死造模的效果比較[J].中醫(yī)正骨,2022,34(03):3-7.
　LOU Pengqiang,DENG Xiaolei,WEI Bo,et al.A comparative analysis of canine femur head necrosis models induced by liquid nitrogen freezing with two freezing durations[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2022,34(03):3-7.
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2種冷凍時長進(jìn)行液氮冷凍法犬股骨頭壞死造模的效果比較()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第34卷
期數(shù):
2022年03期
頁碼:
3-7
欄目:
基礎(chǔ)研究
出版日期:
2022-03-20

文章信息/Info

Title:
A comparative analysis of canine femur head necrosis models induced by liquid nitrogen freezing with two freezing durations
作者:
樓鵬強(qiáng); 鄧小磊; 魏波; 湯朔; 侯德才
(遼寧中醫(yī)藥大學(xué)附屬醫(yī)院,遼寧 沈陽 110032)
Author(s):
LOU Pengqiang; DENG Xiaolei; WEI Bo; TANG Shuo; HOU Decai
The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China
關(guān)鍵詞:
股骨頭壞死;  模型; 動物;  犬;  液氮
Keywords:
femur head necrosis;  models; animal;  canis familiaris;  liquid nitrogen
摘要:
目的:比較2種冷凍時長進(jìn)行液氮冷凍法犬股骨頭壞死造模的效果。方法:將12只SPF級健康成年雄性比格犬隨機(jī)分為2組,每組6只。均采用液氮冷凍法進(jìn)行股骨頭壞死造模,液氮冷凍時長分別為8 min(冷凍8 min組)和4 min(冷凍4 min組)。造模手術(shù)后觀察并記錄動物的一般情況。造模后9周,進(jìn)行X線和MRI檢查,并以MRI影像作為判斷股骨頭壞死的標(biāo)準(zhǔn)。影像學(xué)檢查結(jié)束后,處死動物,取出造模側(cè)股骨頭,進(jìn)行大體觀察和病理學(xué)檢查(HE染色)。結(jié)果:①一般情況。至最終處死前,2組均無動物死亡。造模手術(shù)后2 d內(nèi),所有動物均開始出現(xiàn)飲食減少、精神萎靡、活動減少等表現(xiàn)。冷凍4 min組動物的上述表現(xiàn)均在1周內(nèi)改善; 冷凍8 min組動物的上述表現(xiàn)約在2周后逐步恢復(fù)正常,切口愈合緩慢,而且均出現(xiàn)了跛行現(xiàn)象。冷凍8 min組的1只動物在造模手術(shù)后第3天出現(xiàn)術(shù)區(qū)皮膚紅腫,造模手術(shù)后1周時出現(xiàn)對應(yīng)部位皮膚組織壞死及化膿,造模手術(shù)后4周時愈合。②影像學(xué)檢查結(jié)果。X線片顯示,冷凍8 min組動物造模側(cè)股骨頭均出現(xiàn)了不同程度的變化,如骨質(zhì)硬化、關(guān)節(jié)間隙變窄,但變化不明顯,且部分動物的股骨頭出現(xiàn)了囊性變; 冷凍4 min組僅2只動物的股骨頭出現(xiàn)了輕微的骨質(zhì)硬化。MRI顯示,冷凍8 min組所有動物T2WI上均可見股骨頭骨髓水腫和關(guān)節(jié)腔積液表現(xiàn),T1WI上均可見線樣低信號,均達(dá)到股骨頭壞死標(biāo)準(zhǔn); 冷凍4 min組4只動物T2WI上可見股骨頭骨髓水腫和關(guān)節(jié)腔積液表現(xiàn),其余2只動物的T2WI上僅可見關(guān)節(jié)腔積液表現(xiàn),T1WI上均無明顯改變。③大體觀察結(jié)果。冷凍8 min組股骨頭扁平、表面較粗糙,部分股骨頭出現(xiàn)軟骨裂紋,部分股骨頭軟骨磨損嚴(yán)重; 冷凍4 min組股骨頭形態(tài)無明顯改變,表面較光滑,僅部分動物的股骨頭出現(xiàn)了軟骨裂紋或輕微軟骨磨損。④病理學(xué)檢查結(jié)果。冷凍8 min組股骨頭組織骨陷窩內(nèi)大部分骨細(xì)胞核消失,同時可見骨小梁斷裂; 冷凍4 min組股骨頭組織大部分骨陷窩內(nèi)可見骨細(xì)胞核,骨小梁完整。冷凍8 min組股骨頭組織空骨陷窩率高于冷凍4 min組[(20.21±2.6)%,(13.51±3.1)%,t=4.055,P=0.002]。結(jié)論:以液氮冷凍法進(jìn)行犬股骨頭壞死造模時,液氮冷凍8 min的造模成功率高于液氮冷凍4 min。
Abstract:
Objective:To compare the effects of canine femur head necrosis(FHN)models induced by liquid nitrogen freezing(LNF)with two freezing durations.Methods:Twelve SPF-grade healthy adult male beagle canines were selected and randomly assigned to 2 groups,6 ones in each group,and they were subjected to LNF for 8 minutes(8-min freezing group)and 4 minutes(4-min freezing group)respectively for inducing FHN.The general conditions of the model canines were then observed and recorded after the modeling surgery.At 9 weeks after the modeling,all model canines underwent X-ray examination and magnetic resonance imaging(MRI)examination and the MRI images were used as the standard for confirming whether the FHN models were built successfully.After the end of imaging examination,the model canines were sacrificed and their femur heads were harvested from the modeling side for gross observation and pathological examination(HE staining).Results:①No model canine died in the 2 groups before the final execution.Within 2 days after the modeling surgery,all model canines began to exhibit such symptoms as decreased food intake,mental fatigue and reduced movement,which was improved within 1 week in 4-min freezing group,and gradually returned to normal about 2 weeks later in 8-min freezing group with the symptoms of slow wound healing and claudication.One model canine in 8-min freezing group exhibited the symptoms as skin redness and swelling on the 3rd day after the modeling surgery,skin tissue necrosis and pyosis at the corresponding site at 1 week after the modeling surgery,and healed at 4 weeks after the modeling surgery.②The results of X-ray examination showed that the unremarkable changes in different degrees,manifesting as osteosclerosis and joint space narrowing,were observed in femur heads at the modeling side of model canine in 8-min freezing group,and cystic changes in the femur heads were further observed in some model canines; while the slight osteosclerosis in femur heads was observed in only 2 model canines in 4-min freezing group.As revealed by MRI images,the bone marrow edema(BME)of femur heads and articular cavity effusion on T2WI and cord-like low signals on T1WI were observed in all model canines of 8-min freezing group,which met the diagnosis criteria of FHN; while,for model canines in 4-min freezing group,the BME of femur heads and articular cavity effusion on T2WI were observed in only 4 ones,and articular cavity effusion alone on T2WI were observed in the other 2 ones,and no significant changes on T1WI was found in all model canines.③The flattened femur heads with rough surfaces were observed in model canines of 8-min freezing group,furthermore,some ones presented with cartilage cracks and the other ones with severely worn cartilage; while,for model canines in 4-min freezing group,the femur heads were comparatively smooth without obvious changes in the morphology,just cartilage cracks or slight cartilage wear were observed in only some ones.④Most of the osteocyte nuclei disappeared within the bone lacunae of femoral head tissues in model canines of 8-min freezing group,and the bone trabeculae fractures was observed; while the osteocyte nuclei was observed within most of the bone lacunae of femoral head tissues in model canines of 4-min freezing group,and the bone trabeculae were intact.The percentage of empty bone lacunae in femoral head tissues was higher in 8-min freezing group compared to 4-min freezing group(20.21±2.6 vs 13.51±3.1%,t=4.055,P=0.002).Conclusion:The success rate is higher in canine FHN model induced by LNF for 8 minutes compared to the ones for 4 minutes.

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(收稿日期:2021-11-14 本文編輯:李曉樂)

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備注/Memo

備注/Memo:
基金項目:遼寧省科學(xué)技術(shù)計劃項目(2017225043) 通訊作者:侯德才 E-mail:lnzyhdc@163.com
更新日期/Last Update: 1900-01-01