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[1]葛海雅,鄢來軍,張燕,等.基于網(wǎng)絡(luò)藥理學(xué)方法分析當(dāng)歸-川芎藥對(duì)治療骨關(guān)節(jié)炎的潛在靶點(diǎn)和機(jī)制[J].中醫(yī)正骨,2020,32(09):1-8.
　GE Haiya,YAN Laijun,ZHANG Yan,et al.Analysis of potential targets and mechanism of Danggui-Chuanxiong herb pair for treatment of osteoarthritis using network pharmacology approach[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2020,32(09):1-8.
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基于網(wǎng)絡(luò)藥理學(xué)方法分析當(dāng)歸-川芎藥對(duì)治療骨關(guān)節(jié)炎的潛在靶點(diǎn)和機(jī)制()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第32卷
期數(shù):
2020年09期
頁碼:
1-8
欄目:
基礎(chǔ)研究
出版日期:
2020-09-20

文章信息/Info

Title:
Analysis of potential targets and mechanism of Danggui-Chuanxiong herb pair for treatment of osteoarthritis using network pharmacology approach
作者:
葛海雅1; 鄢來軍1; 張燕2; 耿秋東1; 黃澤靈1; 李楠1
(1.福建中醫(yī)藥大學(xué)中醫(yī)學(xué)院,福建 福州 350122; 2.中醫(yī)骨傷及運(yùn)動(dòng)康復(fù)教育部重點(diǎn)實(shí)驗(yàn)室,福建 福州 350122)
Author(s):
GE Haiya1; YAN Laijun1; ZHANG Yan2; GENG Qiudong1; HUANG Zeling1; LI Nan1
1.College of Traditional Chinese Medicine of Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China2.Key Laboratory of Orthopedics & Traumatology and Rehabilitation of Traditional Chinese Medicine of Ministry of Education,Fuzhou 350122,Fujian,China
關(guān)鍵詞:
骨關(guān)節(jié)炎;  當(dāng)歸;  川芎;  藥對(duì);  網(wǎng)絡(luò)藥理學(xué)
Keywords:
osteoarthritis;  angelica sinensis;  ligusticum chuanxiong;  paired drugs;  network pharmacology
摘要:
目的:探討當(dāng)歸-川芎藥對(duì)治療骨關(guān)節(jié)炎(osteoarthritis,OA)的作用靶點(diǎn)和機(jī)制。方法:通過中藥系統(tǒng)藥理學(xué)數(shù)據(jù)庫與分析平臺(tái)(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)檢索、篩選當(dāng)歸和川芎的活性成分及作用靶點(diǎn)。在GeneCards數(shù)據(jù)庫檢索OA的相關(guān)基因,通過R軟件與當(dāng)歸、川芎的作用靶點(diǎn)相交疊,篩選當(dāng)歸-川芎藥對(duì)治療OA的關(guān)鍵靶點(diǎn)。分別利用Cytoscape軟件和STRING數(shù)據(jù)庫構(gòu)建藥物-成分-靶點(diǎn)-疾病網(wǎng)絡(luò)和關(guān)鍵靶點(diǎn)蛋白相互作用網(wǎng)絡(luò)。最后利用R軟件進(jìn)行關(guān)鍵靶點(diǎn)基因GO功能富集分析與KEGG通路富集分析。結(jié)果:共篩選出當(dāng)歸-川芎藥對(duì)的9個(gè)活性成分及對(duì)應(yīng)的29個(gè)關(guān)鍵靶點(diǎn)。建立的藥物-成分-靶點(diǎn)-疾病網(wǎng)絡(luò)共包含36個(gè)節(jié)點(diǎn)、71條邊,當(dāng)歸的2個(gè)活性成分對(duì)應(yīng)15個(gè)靶點(diǎn),川芎的7個(gè)活性成分對(duì)應(yīng)14個(gè)靶點(diǎn)。建立的靶點(diǎn)蛋白相互作用網(wǎng)絡(luò)包含25個(gè)節(jié)點(diǎn)、102條邊,其中的關(guān)鍵靶點(diǎn)蛋白包括半胱氨酸蛋白酶3(caspase 3,CASP3)、JUN、雌激素受體1(estrogen receptor alpha,ESR1)、前列腺素內(nèi)過氧化物合酶2(prostaglandin-endoperoxide synthase 2,PTGS2)、絲裂原活化蛋白激酶14(mitogen-activated protein kinase 14,MAPK14)。GO功能富集分析與KEGG通路富集分析顯示,當(dāng)歸-川芎藥對(duì)治療OA關(guān)鍵靶點(diǎn)基因的生物學(xué)過程和功能主要集中在核受體功能、轉(zhuǎn)錄因子活性、類固醇激素受體活性、固醇類激素結(jié)合、細(xì)胞凋亡等,相關(guān)的信號(hào)通路主要包括Apoptosis-multiple species信號(hào)通路、白細(xì)胞介素17信號(hào)通路、血管內(nèi)皮生長(zhǎng)因子信號(hào)通路、p53信號(hào)通路等。結(jié)論:當(dāng)歸-川芎藥對(duì)治療OA具有多成分-多靶點(diǎn)-多途徑的特點(diǎn),可能通過CASP3、JUN、ESR1、PTGS2、MAPK14等靶點(diǎn)及Apoptosis-multiple species、白細(xì)胞介素17、血管內(nèi)皮生長(zhǎng)因子、p53等信號(hào)通路發(fā)揮治療作用。
Abstract:
To explore the action targets and mechanism of Danggui-Chuanxiong(DG-CX)herb pair for treatment of osteoarthritis(OA).Methods:The active ingredients and action targets of Danggui and Chuanxiong were screened by retrieving Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The OA-related genes were searched out from GeneCards databases,and the key targets of DG-CX herb pair for treatment of OA were selected out through overlapping OA-related genes with action targets of Danggui and Chuanxiong respectively using R software.The drug-ingredient-target-disease network was built by using Cytoscape software,and the network of interaction between key target proteins was built by using STRING database.GO function and KEGG pathway enrichment analysis were performed on key target proteins respectively by using R software.Results:Nine active ingredients and 29 corresponding key targets of DG-CX herb pair were selected out.The drug-ingredient-target-disease network diagram consists of 36 nodes and 71 edges in total,in which the 2 active ingredients of Danggui corresponded to 15 targets and the 7 active ingredients of Chuanxiong corresponded to 14 targets.The network of interaction between target proteins consists of 25 nodes and 102 edges in total,in which the key target proteins included caspase 3(CASP3),JUN,estrogen receptor alpha(ESR1),prostaglandin-endoperoxide synthase 2(PTGS2)and mitogen-activated protein kinase 14(MAPK14).The results of GO function and KEGG pathway enrichment analysis demonstrated that the biological processes and functions of key target genes of DG-CX herb pair for treatment of OA were mainly concentrated in nuclear receptor function,transcription factor activity,steroid hormone receptor activity,steroid hormone bind and cell apoptosis; and the related signal pathways consisted of apoptosis-multiple species signal pathway,interleukin-17(IL-17)signal pathway,vascular endothelial growth factor(VEGF)signal pathway and p53 signal pathway.Conclusion:The DG-CX herb pair has the characteristics of multiple ingredients,multiple targets and multiple pathways in treatment of OA,and it may produce the therapeutic effects through the targets including CASP3,JUN,ESR1,PTGS2 and MAPK14 and the signal pathways including Apoptosis-multiple species,IL-17,VEGF and p53 signal pathway.

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備注/Memo

備注/Memo:
基金項(xiàng)目:福建省自然科學(xué)基金資助項(xiàng)目(2019J01349)
通訊作者:李楠 E-mail:[email protected]
更新日期/Last Update: 2020-09-20