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[1]張麟,向楠,周廣文,等.補(bǔ)腎化痰方對去卵巢大鼠骨形成的影響及其作用機(jī)制研究[J].中醫(yī)正骨,2018,30(10):12-18.
　ZHANG Lin,XIANG Nan,ZHOU Guangwen,et al.Effect of Bushen Huatan Fang(補(bǔ)腎化痰方)on bone formation of ovariectomized rats and its mechanism of action[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2018,30(10):12-18.
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補(bǔ)腎化痰方對去卵巢大鼠骨形成的影響及其作用機(jī)制研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第30卷
期數(shù):
2018年10期
頁碼:
12-18
欄目:
基礎(chǔ)研究
出版日期:
2018-10-20

文章信息/Info

Title:
Effect of Bushen Huatan Fang(補(bǔ)腎化痰方)on bone formation of ovariectomized rats and its mechanism of action
作者:
張麟1; 向楠2; 周廣文2; 周亞娜3; 胡婭4; 張妍2; 李章青2
(1.湖北省武漢市第一醫(yī)院,湖北 武漢 430022; 2.湖北中醫(yī)藥大學(xué),湖北 武漢 430061; 3.湖北省中醫(yī)院,湖北 武漢 430061; 4.長江大學(xué),湖北 荊州 434023)
Author(s):
ZHANG Lin1; XIANG Nan2; ZHOU Guangwen2; ZHOU Yana3; HU Ya4; ZHANG Yan2; LI Zhangqing2
1.The First Hospital of Wuhan,Wuhan 430022,Hubei,China 2.Hubei University of Traditional Chinese Medicine,Wuhan 430061,Hubei,China 3.Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,Hubei,China 4.Changjiang University,Jingzhou 434023,Hubei,China
關(guān)鍵詞:
骨質(zhì)疏松;  補(bǔ)腎化痰方;  信號傳導(dǎo);  骨形態(tài)發(fā)生蛋白質(zhì)2;  Runt相關(guān)轉(zhuǎn)錄因子2;  大鼠; Sprague-Dawley;  動物實驗
Keywords:
osteoporosis;  Bushen Huatan Fang;  signal transduction;  bone morphogenetic protein 2;  Runt related transcription factor 2;  rats; Sprague-Dawley;  animal experimentation
摘要:
目的:觀察補(bǔ)腎化痰方對去卵巢大鼠骨形成的影響及其作用機(jī)制。方法:將30只6月齡SPF級雌性SD大鼠隨機(jī)分為假手術(shù)組、模型組和補(bǔ)腎化痰組,每組10只。模型組和補(bǔ)腎化痰組大鼠手術(shù)摘除雙側(cè)卵巢,假手術(shù)組不摘除卵巢,只切除卵巢周圍與雙側(cè)卵巢質(zhì)量相等的脂肪組織。造模術(shù)后5周開始,補(bǔ)腎化痰組以0.94 g·mL-1補(bǔ)腎化痰方藥液灌胃,假手術(shù)組和模型組以等體積生理鹽水灌胃,均每天1次,連續(xù)干預(yù)8周。藥物干預(yù)結(jié)束后,將所有大鼠處死,摘除雙側(cè)眼球取血檢測血清骨形態(tài)發(fā)生蛋白(bone morphogenetic protein,BMP)-2、Runt相關(guān)轉(zhuǎn)錄因子(Runt related transcription factor,RUNX)-2含量,取左側(cè)股骨行Micro CT掃描測定骨密度、骨小梁數(shù)量、骨小梁平均厚度、骨小梁分離度,取右側(cè)股骨上端部分骨片以蛋白免疫印跡法測定骨組織中BMP-2、RUNX-2含量,取右側(cè)股骨下端部分骨片以免疫組織化學(xué)法檢測BMP-2、RUNX-2的定位。結(jié)果:①一般情況。所有大鼠均在造模手術(shù)麻醉3～4 h后完全清醒,正常進(jìn)食,1周后切口完全愈合。藥物干預(yù)過程中,模型組1只大鼠死亡、補(bǔ)腎化痰組2只大鼠死亡。②血清BMP-2、RUNX-2含量檢測結(jié)果。3組大鼠的血清BMP-2含量比較,差異有統(tǒng)計學(xué)意義[(245.40±10.10)pg·mL-1,(111.03±29.75)pg·mL-1,(190.35±14.00)pg·mL-1,F=109.998,P=0.000]; 模型組、補(bǔ)腎化痰組的血清BMP-2含量均低于假手術(shù)組(P=0.000,P=0.000),補(bǔ)腎化痰組的血清BMP-2含量高于模型組(P=0.000)。3組大鼠的血清RUNX-2含量比較,差異有統(tǒng)計學(xué)意義[(4 131.04±277.39)pg·mL-1,(1 601.09±266.10)pg·mL-1,(3 134.90±202.33)pg·mL-1,F=237.192,P=0.000]; 模型組、補(bǔ)腎化痰組的血清RUNX-2含量均低于假手術(shù)組(P=0.000,P=0.000),補(bǔ)腎化痰組的血清RUNX-2含量高于模型組(P=0.000)。③股骨Micro CT掃描結(jié)果。3組大鼠骨密度比較,差異有統(tǒng)計學(xué)意義[(556.90±8.86)mg HA·cm-3,(498.81±9.26)mg HA·cm-3,(530.17±10.70)mg HA·cm-3,F=90.488,P=0.000]; 模型組、補(bǔ)腎化痰組的骨密度均低于假手術(shù)組(P=0.000,P=0.000),補(bǔ)腎化痰組的骨密度高于模型組(P=0.000)。3組大鼠骨小梁數(shù)量比較,差異有統(tǒng)計學(xué)意義[(2.65±0.32)個·mm-1,(1.74±0.22)個·mm-1,(2.41±0.33)個·mm-1,F=23.900,P=0.000]; 模型組的骨小梁數(shù)量少于補(bǔ)腎化痰組和假手術(shù)組(P=0.000,P=0.000),補(bǔ)腎化痰組的骨小梁數(shù)量與假手術(shù)組比較,差異無統(tǒng)計學(xué)意義(P=0.224)。3組大鼠骨小梁平均厚度比較,差異有統(tǒng)計學(xué)意義[(109.33±16.12)μm,(79.27±18.17)μm,(95.73±11.38)μm,F=8.730,P=0.001]; 模型組的骨小梁平均厚度低于假手術(shù)組(P=0.001),補(bǔ)腎化痰組的骨小梁平均厚度分別與假手術(shù)組和模型組比較,差異無統(tǒng)計學(xué)意義(P=0.181,P=0.098)。3組大鼠骨小梁分離度比較,差異有統(tǒng)計學(xué)意義[(350.95±68.46)μm,(472.33±33.70)μm,(406.68±47.77)μm,F=12.460,P=0.000]; 模型組、補(bǔ)腎化痰組的骨小梁分離度均高于假手術(shù)組(P=0.000,P=0.036),補(bǔ)腎化痰組的骨小梁分離度低于模型組(P=0.017)。④股骨組織中BMP-2、RUNX-2蛋白含量檢測結(jié)果。3組大鼠股骨組織中的BMP-2含量比較,差異有統(tǒng)計學(xué)意義(0.73±0.03,0.26±0.02,0.48±0.02,F=738.111,P=0.000); 模型組、補(bǔ)腎化痰組股骨組織中的BMP-2含量均低于假手術(shù)組(P=0.000,P=0.000),補(bǔ)腎化痰組股骨組織中的BMP-2含量高于模型組(P=0.000)。3組大鼠股骨組織中的RUNX-2含量比較,差異有統(tǒng)計學(xué)意義(0.67±0.03,0.36±0.03,0.47±0.02,F=286.493,P=0.000); 模型組、補(bǔ)腎化痰組股骨組織中的RUNX-2含量均低于假手術(shù)組(P=0.000,P=0.000),補(bǔ)腎化痰組股骨組織中的RUNX-2含量高于模型組(P=0.000)。⑤股骨組織中BMP-2、RUNX-2蛋白定位檢測結(jié)果。BMP-2、RUNX-2的陽性表達(dá)主要位于骨髓基質(zhì)細(xì)胞胞漿及骨小梁周邊成骨細(xì)胞中,光學(xué)顯微鏡下陽性表達(dá)蛋白呈棕黃色顆粒。結(jié)論:補(bǔ)腎化痰方能通過調(diào)節(jié)BMP-2/Smads/RUNX-2信號轉(zhuǎn)導(dǎo)通路,促進(jìn)去卵巢大鼠的骨形成。
Abstract:
Objective:To observe the effects of Bushen Huatan Fang(補(bǔ)腎化痰方,BSHTF)on bone formation of ovariectomized rats and its mechanism of action.Methods:Thirty 6-month-old SPF-grade female SD rats were randomly divided into sham-operated group,model group and BSHTF group,10 cases in each group.The bilateral ovariectomy were performed on rats in model group and BSHTF group,and the resection of same mass of fat around bilateral ovaries were performed on rats in sham-operated group.Since the 5th week after the modeling operation,the rats in BSHTF group were intragastric administrated with BSHTF solution in dosages of 0.94 g/mL,while the rats in sham-operated group and model group were intragastric administrated with the same dose of normal saline,once a day for consecutive 8 weeks.After the end of drug intervention,all rats were executed and their bilateral eyeballs were removed and their blood were fetched out for detecting the serum contents of bone morphogenetic protein(BMP)-2 and Runt related transcription factor(RUNX)-2.Their left femurs were fetched out for determining bone mineral density(BMD),trabecular number(Tb.N),mean trabecular thickness(Tb.Th)and trabecular separation(Tb.Sp)through Micro-CT scanning.Meanwhile,the sclerites were fetched out from the upper end and the lower end of right femurs respectively for measuring the contents of BMP-2 and RUNX-2 in bone tissues by using Western blot assay and detecting the location of BMP-2 and RUNX-2 by using immunohistochemical method.Results:All rats were fully waked up from 3-4-hour anesthesia for the modeling surgery and fed normally,and their incisions healed completely 1 week later.One rat in model group and 2 rats in BSHTF group died during the drug intervention.The detection results of serum contents of BMP-2 and RUNX-2 showed that there was statistical difference in serum contents of BMP-2 between the 3 groups(245.40+/-10.10,111.03+/-29.75,190.35+/-14.00 pg/mL,F=109.998,P=0.000).The serum contents of BMP-2 were lower in model group and BSHTF group compared to sham-operated group,and were higher in BSHTF group compared to model group(P=0.000,P=0.000; P=0.000).There was statistical difference in serum contents of RUNX-2 between the 3 groups(4 131.04+/-277.39,1 601.09+/-266.10,3 134.90+/-202.33 pg/mL,F=237.192,P=0.000).The serum contents of RUNX-2 were lower in model group and BSHTF group compared to sham-operated group,and were higher in BSHTF group compared to model group(P=0.000,P=0.000; P=0.000).The results of Micro-CT scanning on femurs showed that there was statistical difference in the BMD between the 3 groups(556.90+/-8.86,498.81+/-9.26,530.17+/-10.70 mg HA/cm(-3),F=90.488,P=0.000).The BMD was lower in model group and BSHTF group compared to sham-operated group,and was higher in BSHTF group compared to model group(P=0.000,P=0.000; P=0.000).There was statistical difference in Tb.N between the 3 groups(2.65+/-0.32,1.74+/-0.22,2.41+/-0.33 /mm,F=23.900,P=0.000).The Tb.N was less in model group compared to BSHTF group and sham-operated group(P=0.000,P=0.000),and there was no statistical difference in Tb.N between BSHTF group and sham-operated group(P=0.224).There was statistical difference in mean Tb.Th between the 3 groups(109.33+/-16.12,79.27+/-18.17,95.73+/-11.38 μm,F=8.730,P=0.001).The mean Tb.N was less in model group compared to sham-operated group(P=0.001),and there was no statistical difference in mean Tb.N between BSHTF group and sham-operated group and between BSHTF group and model group(P=0.181,P=0.098).There was statistical difference in Tb.Sp between the 3 groups(350.95+/-68.46,472.33+/-33.70,406.68+/-47.77 μm,F=12.460,P=0.000).The Tb.Sp was higher in model group and BSHTF group compared to sham-operated group,and was lower in BSHTF group compared to model group(P=0.000,P=0.036; P=0.017).The detection results of contents of BMP-2 and RUNX-2 protein in femoral tissues showed that there was statistical difference in content of BMP-2 in femoral tissues between the 3 groups(0.73+/-0.03,0.26+/-0.02,0.48+/-0.02,F=738.111,P=0.000).The contents of BMP-2 in femoral tissues were lower in model group and BSHTF group compared to sham-operated group,and were higher in BSHTF group compared to model group(P=0.000,P=0.000; P=0.000).There was statistical difference in content of RUNX-2 in femoral tissues between the 3 groups(0.67+/-0.03,0.36+/-0.03,0.47+/-0.02,F=286.493,P=0.000).The contents of RUNX-2 in femoral tissues were lower in model group and BSHTF group compared to sham-operated group,and were higher in BSHTF group compared to model group(P=0.000,P=0.000; P=0.000).The detection results of location of BMP-2 and RUNX-2 protein in femoral tissues showed that the positive expressions of BMP-2 and RUNX-2 were mainly found in cytoplasms of bone marrow stromal cells and osteoblasts around bone trabecula,and the positive expressed protein presented with brown-yellow granules under the optical microscope.Conclusion:BSHTF can promote the bone formation of ovariectomized rats through regulating BMP-2/Smads/RUNX-2 signal transduction pathway.

參考文獻(xiàn)/References:


[1] 吳海洋,索歡,王平.絕經(jīng)后骨質(zhì)疏松癥的臨床中藥治療進(jìn)展[J].中國骨質(zhì)疏松雜志,2015,21(2):241-244.
[2] TIAN L,YU XJ.Lipid metabolism disorders and bone dysfunction-interrelated and mutually regulated(Review)[J].Mol Med Rep,2015,12(1):783-794.
[3] 周亞娜,向楠,陳輝.補(bǔ)腎化痰法影響骨髓間充質(zhì)干細(xì)胞成骨分化的實驗研究[J].中國中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志,2015,21(3):275-277.
[4] 胡婭,向楠,唐曦,等.補(bǔ)腎化痰方對去卵巢大鼠血清TRACP5b和β-CTX的影響[J].現(xiàn)代生物醫(yī)學(xué)進(jìn)展,2011,11(12):2227-2229.
[5] 查小云,胡予.骨質(zhì)疏松相關(guān)信號通路研究進(jìn)展[J].中國骨質(zhì)疏松雜志,2014,37(2):205-209.
[6] JAVED A,AFZAL F,BAE JS,et al.Specific residues of RUNX2 are obligatory for formation of BMP2-induced RUNX2-SMAD complex to promote osteoblast differentiation[J].Cells Tissues Organs,2009,189(1/4):133-137.
[7] KOMORI T.Regulation of bone development and extracellular matrix protein genes by RUNX2[J].Cell Tissue Res,2010,339(1):189-195.
[8] 於紹龍,劉丹平.成骨因子BMP-2/Smads/Runx2信號轉(zhuǎn)導(dǎo)通路[J].錦州醫(yī)科大學(xué)學(xué)報, 2014,35(5):94-96.
[9] 劉政,吳倩,黃帥立.骨質(zhì)疏松癥的中醫(yī)病因病機(jī)與治則研究[J].江蘇中醫(yī)藥,2014,46(2):42-44.
[10] 葛繼榮,鄭洪新,萬小明,等.中醫(yī)藥防治原發(fā)性骨質(zhì)疏松癥專家共識(2015)[J].中國骨質(zhì)疏松雜志,2015,21(9):1023-1028.
[11] 第五永長.老年性癡呆“髓空痰濁”病機(jī)及治法再識[J].中醫(yī)雜志,2016,57(12):1075-1077.
[12] 丁寧,張梅,張琳麗,等.脂代謝紊亂的中醫(yī)藥研究進(jìn)展[J].中國老年學(xué)雜志,2015,35(2):549-552.
[13] 李維娜,馮玲,隋歌川.從痰濁論治高脂血癥研究進(jìn)展[J].山東中醫(yī)雜志,2017,36(5):430-433.
[14] 張冰雨,曾瑞翔,雷濤.脂代謝與骨代謝相關(guān)機(jī)制的研究進(jìn)展[J].中國骨質(zhì)疏松雜志,2015,21(10):1263-1266.
[15] 蘇甜,羅湘杭.血脂紊亂與骨代謝[J].藥物與臨床,2015,12(15):27-33.
[16] 沈蘊(yùn)茹.載脂蛋白E的基因多樣性對脂代謝及骨代謝的影響[J].中國骨質(zhì)疏松雜志,2017,23(10):1353-1358.
[17] 陳曉,蘇佳燦.骨質(zhì)疏松研究熱點(diǎn):骨髓間充質(zhì)干細(xì)胞分化命運(yùn)[J].第二軍醫(yī)大學(xué)學(xué)報,2017, 38(4):397-404.
[18] 丁文鴿.卵巢切除骨質(zhì)疏松小鼠骨折愈合不同時期骨微結(jié)構(gòu)及力學(xué)性能變化[J].中國組織工程研究與臨床康復(fù),2008,12(42):8247-8250.
[19] DAVISON KS,KENDLER DL,AMMANN P,et al.Assessing fracture risk and effects of osteoporosis drugs:bone mineral density and beyond[J].Am J Med,2009,122(11):992-997.

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[7]項旻,楊虹,林愛菊,等.絕經(jīng)后2型糖尿病患者骨質(zhì)疏松與血微量元素的關(guān)系研究[J].中醫(yī)正骨,2013,25(12):20.
　Xiang Min*,Yang Hong,Lin Aiju,et al.Clinical study on the relationship between osteoporosis and serum trace elements levels in postmenopausal women with type 2 diabetes[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2013,25(10):20.
[8]史曉林,李春雯,張志強(qiáng).弱陽離子磁珠分離技術(shù)和基質(zhì)輔助激光解吸電離飛行時間質(zhì)譜技術(shù)在原發(fā)性Ⅰ型骨質(zhì)疏松癥血清標(biāo)志蛋白篩選中的應(yīng)用[J].中醫(yī)正骨,2014,26(03):5.
　Shi Xiaolin*,Li Chunwen,Zhang Zhiqiang..Application of magnetic beads based weak cation exchange separation technology and matrix-assisted laser desorption-ionization time of flight mass spectrometry technology in screening serum protein markers of primary type-Ⅰ osteoporosis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(10):5.
[9]李明,徐明雄,馮左基,等.自擬壯骨方治療絕經(jīng)后骨質(zhì)疏松癥的療效及作用機(jī)制研究[J].中醫(yī)正骨,2014,26(09):21.
　Li Ming*,Xu Mingxiong,Feng Zuoji,et al.Study on the curative effect and mechanism of action of self-made ZHUANGGU decoction in treatment of postmenopausal osteoporosis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(10):21.
[10]陳俊杰,李晴晴,夏瑢.脂代謝及血清內(nèi)脂素水平與絕經(jīng)后骨質(zhì)疏松癥的 相關(guān)性研究[J].中醫(yī)正骨,2012,24(04):16.
　CHEN Jun-jie*,LI Qing-qing,XIA Rong.*.Study on the correlations between the levels of lipid metabolism and serum visfatin and postmenopausal osteoporosis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(10):16.

備注/Memo

備注/Memo:
基金項目:湖北省衛(wèi)生廳中醫(yī)藥中西醫(yī)結(jié)合科研項目(2012Z-Y31); 湖北中醫(yī)藥大學(xué)“青苗計劃”資助項目(2016ZZX016)
通訊作者:向楠 E-mail:[email protected]
更新日期/Last Update: 2019-02-25