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[1]陶振宇,張?jiān)吕?陳華,等.細(xì)胞自噬在酒精性股骨頭壞死中的作用及相關(guān)機(jī)制研究[J].中醫(yī)正骨,2018,30(10):4-11.
　TAO Zhenyu,ZHANG Yuelei,CHEN Hua,et al.Roles of cell autophagy in alcohol-induced osteonecrosis of the femoral head and its related mechanisms of action:an experimental study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2018,30(10):4-11.
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細(xì)胞自噬在酒精性股骨頭壞死中的作用及相關(guān)機(jī)制研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第30卷
期數(shù):
2018年10期
頁(yè)碼:
4-11
欄目:
股骨頭壞死
出版日期:
2018-10-20

文章信息/Info

Title:
Roles of cell autophagy in alcohol-induced osteonecrosis of the femoral head and its related mechanisms of action:an experimental study
作者:
陶振宇; 陳華; 孫遼軍
(溫州醫(yī)科大學(xué)附屬第二醫(yī)院,浙江 溫州 325027)
Author(s):
TAO Zhenyu; ZHANG Yuelei; CHEN Hua; SUN Liaojun
The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,Zhejiang,China
關(guān)鍵詞:
股骨頭壞死;  自噬;  西羅莫司;  小鼠;  動(dòng)物實(shí)驗(yàn)
Keywords:
femur head necrosis;  autophagy;  sirolimus;  mice;  animal experimentation
摘要:
目的:探討細(xì)胞自噬在酒精性股骨頭壞死中的作用及相關(guān)機(jī)制。方法:將15只1月齡SPF級(jí)C57小鼠隨機(jī)分為空白對(duì)照組、酒精組及雷帕霉素-酒精組,每組5只。空白對(duì)照組喂食飲用水6周,腹腔內(nèi)注射5 mg·kg-1不含雷帕霉素的溶劑; 酒精組喂食含30%酒精的飲用水6周,腹腔內(nèi)注射5 mg·kg-1不含雷帕霉素的溶劑; 雷帕霉素-酒精組喂食含30%酒精的飲用水6周,腹腔內(nèi)注射5 mg·kg-1的雷帕霉素溶液。腹腔內(nèi)注射每天1次,連續(xù)注射2周。酒精干預(yù)6周后,獲取各組小鼠右側(cè)股骨頭,行微型CT平掃后將股骨頭制成切片,蘇木素-伊紅染色后在顯微鏡下觀察股骨頭組織形態(tài),并計(jì)算空骨陷窩百分比(總細(xì)胞凋亡率)。取MC3T3-E1細(xì)胞,傳代培養(yǎng)至第3代,經(jīng)含酒精濃度為0 mmol·L-1、25 mmol·L-1、50 mmol·L-1、100 mmol·L-1、200 mmol·L-1的α-MEM培養(yǎng)基孵育后,采用CCK8法檢測(cè)其相對(duì)生長(zhǎng)率。將培養(yǎng)好的第3代MC3T3-E1細(xì)胞隨機(jī)分為4組,分別以普通α-MEM培養(yǎng)基(正常組)、含100 mmol·L-1酒精的α-MEM培養(yǎng)基(含酒精組)、含100 nmol·L-1雷帕霉素的α-MEM培養(yǎng)基(含雷帕霉素組)、含100 mmol·L-1酒精和100 nmol·L-1雷帕霉素的α-MEM培養(yǎng)基(含酒精-雷帕霉素組)培養(yǎng),連續(xù)培養(yǎng)24 h; 采用Western blot法檢測(cè)MC3T3-E1細(xì)胞中微管相關(guān)蛋白1輕鏈3(microtubule-associated protein 1 light chain 3,LC3)-Ⅰ、LC3-Ⅱ、哺乳動(dòng)物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)、Beclin1蛋白表達(dá)量。結(jié)果:①股骨頭CT掃描結(jié)果。空白對(duì)照組小鼠股骨頭骨小梁大體形態(tài)良好,分布均勻,未見(jiàn)軟骨下壞死帶形成; 酒精組小鼠股骨頭軟骨下壞死帶形成,骨小梁閉塞,髓腔內(nèi)骨小梁變細(xì)、稀疏、斷裂,骨皮質(zhì)增厚; 雷帕霉素-酒精組小鼠股骨頭CT影像學(xué)表現(xiàn)介于酒精組與空白對(duì)照組之間,可見(jiàn)髓腔內(nèi)部分骨小梁出現(xiàn)斷裂萎縮,軟骨下骨部分壞死形成。②顯微鏡下股骨頭組織形態(tài)觀察結(jié)果。空白對(duì)照組小鼠股骨頭骨小梁形態(tài)良好,未見(jiàn)空骨陷窩、脂肪細(xì)胞及脂質(zhì)沉積,無(wú)組織壞死、纖維化; 酒精組小鼠股骨頭骨小梁稀疏、紊亂,脂肪細(xì)胞增多,骨細(xì)胞內(nèi)脂質(zhì)沉積,可見(jiàn)大量空骨陷窩形成,局部組織壞死、纖維化,細(xì)胞數(shù)目減少; 雷帕霉素-酒精組小鼠股骨頭部分骨小梁出現(xiàn)斷裂、萎縮,可見(jiàn)少量空骨陷窩形成。③總細(xì)胞凋亡率。空白對(duì)照組總細(xì)胞凋亡率為(6.06±1.93)%,酒精組為(63.9±9.63)%,雷帕霉素-酒精組為(34.0±5.82)%; 3組總細(xì)胞凋亡率比較,差異有統(tǒng)計(jì)學(xué)意義(F=192.800,P=0.000); 進(jìn)一步兩兩比較,酒精組和雷帕霉素-酒精組總細(xì)胞凋亡率均大于空白對(duì)照組(P=0.000,P=0.000),酒精組總細(xì)胞凋亡率大于雷帕霉素-酒精組(P=0.000)。④不同濃度酒精干預(yù)后MC3T3-E1細(xì)胞相對(duì)生長(zhǎng)率。在含酒精濃度為0 mmol·L-1、25 mmol·L-1、50 mmol·L-1、100 mmol·L-1、200 mmol·L-1的α-MEM培養(yǎng)基中培養(yǎng)24 h后MC3T3-E1細(xì)胞相對(duì)生長(zhǎng)率比較,差異有統(tǒng)計(jì)學(xué)意義(1.00±0.00,0.88±0.04,0.67±0.09,0.31±0.04,0.28±0.02; F=106.900,P=0.001)。未經(jīng)酒精干預(yù)的MC3T3-E1細(xì)胞相對(duì)生長(zhǎng)率高于濃度為25 mmol·L-1、50 mmol·L-1、100 mmol·L-1、200 mmol·L-1的酒精干預(yù)后的MC3T3-E1細(xì)胞相對(duì)生長(zhǎng)率(P=0.011,P=0.000,P=0.000,P=0.000)。⑤MC3T3-E1細(xì)胞中LC3-Ⅱ/LC3-Ⅰ的比值及mTOR、Beclin-1的蛋白表達(dá)。4組小鼠MC3T3-E1細(xì)胞中LC3-Ⅱ/LC3-Ⅰ的比值比較,組間差異有統(tǒng)計(jì)學(xué)意義(1.16±0.10,0.71±0.06,1.50±0.06,1.23±0.05; F=86.600,P=0.000); 正常組MC3T3-E1細(xì)胞中LC3-Ⅱ/LC3-Ⅰ的比值高于含酒精組(P=0.000),低于含雷帕霉素組(P=0.000),與含酒精-雷帕霉素組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.166); 含酒精組MC3T3-E1細(xì)胞中LC3-Ⅱ/LC3-Ⅰ的比值低于含雷帕霉素組及含酒精-雷帕霉素組(P=0.000,P=0.000); 含雷帕霉素組LC3-Ⅱ/LC3-Ⅰ的比值高于含酒精-雷帕霉素組(P=0.000)。4組小鼠MC3T3-E1細(xì)胞中mTOR蛋白表達(dá)量比較,組間差異有統(tǒng)計(jì)學(xué)意義(1.13±0.10,1.23±0.06,0.34±0.03,0.63±0.05; F=167.800,P=0.000); 正常組MC3T3-E1細(xì)胞中mTOR蛋白表達(dá)量低于含酒精組(P=0.033),高于含雷帕霉素組及含酒精-雷帕霉素組(P=0.000,P=0.000); 含酒精組MC3T3-E1細(xì)胞中mTOR蛋白表達(dá)量高于含雷帕霉素組及含酒精-雷帕霉素組(P=0.000,P=0.000),含雷帕霉素組mTOR蛋白表達(dá)量低于含酒精-雷帕霉素組(P=0.000)。4組小鼠MC3T3-E1細(xì)胞中Beclin-1蛋白表達(dá)量比較,組間差異有統(tǒng)計(jì)學(xué)意義(0.97±0.08,0.67±0.01,1.11±0.08,1.06±0.04; F=42.400,P=0.000); 正常組MC3T3-E1細(xì)胞中Beclin-1蛋白表達(dá)量高于含酒精組,而與含雷帕霉素組、含酒精-雷帕霉素組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.077,P=0.127); 含酒精組MC3T3-E1細(xì)胞中Beclin-1蛋白表達(dá)量低于 含雷帕霉素組及含酒精-雷帕霉素組(P=0.000,P=0.000); 含雷帕霉素組MC3T3-E1細(xì)胞中Beclin-1蛋白表達(dá)量高于含酒精-雷帕霉素組(P=0.004)。結(jié)論:細(xì)胞自噬在酒精性股骨頭壞死的病變過(guò)程中可能發(fā)揮著非常重要的保護(hù)作用。酒精刺激引起的細(xì)胞自噬水平的降低可能是引起股骨頭壞死的原因之一,其作用機(jī)制可能與酒精刺激影響自噬關(guān)鍵調(diào)控分子mTOR、Beclin1的表達(dá)有關(guān)。
Abstract:
Objective:To explore the roles of cell autophagy in alcohol-induced osteonecrosis of the femoral head(ONFH)and its related mechanisms of action.Methods:Fifteen 1-month-old SPF-grade C57 mice were randomly divided into blank control group,alcohol group and rapamycin(RAPA)-alcohol group,5 mice in each group.The mice in blank control group,alcohol group and RAPA-alcohol group were fed with drinking water,drinking water added with 30% alcohol and drinking water added with 30% alcohol for 6 weeks respectively,and were intraperitoneal injected with solvent(5 mg/kg)without RAPA,solvent(5 mg/kg)without RAPA and RAPA solution(5 mg/kg)respectively.The intraperitoneal injection was performed once a day for consecutive 2 weeks.After six-week alcohol intervention,the right femoral heads were obtained from mice of each group and were sectioned after micro-CT plain scanning.The tissue morphology of femoral heads were observed under microscope after hematoxylin-eosin(HE)staining,and the percentage of empty bone lacuna(total cell apoptosis rate)was calculated.MC3T3-E1 cells were fetched out from femoral head tissues for subculturing.The relative growth rate(RGR)of the third-generation MC3T3-E1 cells was measured by using CCK8 method after the cells were cultured in α-MEM culture medium supplemented with alcohol with concentrations of 0,25,50,100 and 200 mmol/L respectively.The third-generation MC3T3-E1 cells were randomly divided into 4 groups and were cultured for continuous 24 hours in normal α-MEM culture medium(normal group),α-MEM culture medium supplemented with alcohol with concentration of 100 mmol/L(alcoholic group),α-MEM culture medium supplemented with RAPA with concentration of 100 nmol/L(RAPA group),and α-MEM culture medium supplemented with alcohol and RAPA with concentration of 100 mmol/L and 100 nmol/L(alcoholic-RAPA group)respectively.The protein expressions of microtubule-associated protein 1 light chain 3(LC3)-Ⅰ,LC3-Ⅱ,mammalian target of rapamycin(mTOR)and Beclin1 in MC3T3-E1 cells were detected by using Western blot assays.Results:The results of CT scanning on femoral heads showed that(1)the trabecular bones had good gross morphology and were uniformly distributed,and no subchondral necrotic zone was found in femoral heads in blank control group;(2)the subchondral necrotic zone,occluded trabecular bones,incrassated bone cortex and thin,sparse and fractured intramedullary trabecular bones were found in femoral heads in alcoholic group;(3)the CT imaging manifestations of femoral heads of mice in RAPA-alcohol group were between alcoholic group's and blank control group's,and some fractured and atrophic trabecular bones were found in medullary cavity and partial osteonecrosis of subcartilaginous bone formed.The results of morphological observation on femoral heads under the microscope showed that(1)the trabecular bone had good morphology and no empty bone lacuna,adipocyte,lipid deposition,tissue necrosis and fibrosis were found in femoral heads in blank control group;(2)the trabecular bones were sparse and disordered,and the adipocytes increased in number,and lipidoses was found in boen cells,meanwhile,a large number of empty bone lacuna formed,and necrosis and fibrosis were found in partial tissues,and cells decreased in number in femoral heads in alcoholic group;(3)some fractured and atrophic trabecular bones and a small number of empty bone lacuna were found in femoral heads in RAPA-alcohol group.The total cell apoptosis rates of blank control group,alcohol group and RAPA-alcohol group were 6.06+/-1.93,63.9+/-9.63 and 34.0+/-5.82% respectively.There was statistical difference in the total cell apoptosis rates between the 3 groups(F=192.800,P=0.000).Further pairwise comparison showed that the total cell apoptosis rates were higher in alcohol group and RAPA-alcohol group compared to blank control group,and were higher in alcohol group compared to RAPA-alcohol group(P=0.000,P=0.000; P=0.000).After 24-hour cultivation in α-MEM culture medium supplemented with alcohol with concentrations of 0,25,50,100 and 200 mmol/L,there was statistical difference in RGR of MC3T3-E1 cells(1.00+/-0.00,0.88+/-0.04,0.67+/-0.09,0.31+/-0.04,0.28+/-0.02; F=106.900,P=0.001).The RGR of MC3T3-E1 cells without alcohol intervention was higher than that of MC3T3-E1 cells intervened by alcohol with concentrations of 25,50,100 and 200 mmol/L(P=0.011,P=0.000,P=0.000,P=0.000).There was statistical difference in the ratio of LC3-Ⅱ to LC3-Ⅰin MC3T3-E1 cells of mice between the 4 groups(1.16+/-0.10,0.71+/-0.06,1.50+/-0.06,1.23+/-0.05; F=86.600,P=0.000).The ratio of LC3-Ⅱ to LC3-Ⅰin MC3T3-E1 cells was lower in alcoholic group and was higher in RAPA group compared to normal group(P=0.000; P=0.000),and there was no statistical difference between normal group and alcoholic-RAPA group(P=0.166).The ratio of LC3-Ⅱ to LC3-Ⅰin MC3T3-E1 cells was lower in alcoholic group compared to RAPA group and alcoholic-RAPA group,and was higher in RAPA group compared to alcoholic-RAPA group(P=0.000,P=0.000; P=0.000).There was statistical difference in the protein expression of mTOR in MC3T3-E1 cells between the 4 groups(1.13+/-0.10,1.23+/-0.06,0.34+/-0.03,0.63+/-0.05; F=167.800,P=0.000).The protein expression of mTOR in MC3T3-E1 cells was higher in alcoholic group and was lower in RAPA group and alcoholic-RAPA group compared to normal group(P=0.033; P=0.000,P=0.000).The protein expression of mTOR in MC3T3-E1 cells was higher in alcoholic group compared to RAPA group and alcoholic-RAPA group,and was lower in RAPA group compared to alcoholic-RAPA group(P=0.000,P=0.000; P=0.000).There was statistical difference in the protein expression of Beclin-1 in MC3T3-E1 cells between the 4 groups(0.97+/-0.08,0.67+/-0.01,1.11+/-0.08,1.06+/-0.04; F=42.400,P=0.000).The protein expression of Beclin-1 in MC3T3-E1 cells was higher in normal group compared to alcoholic group,and there was no statistical difference between normal group and RAPA group and between normal group and alcoholic-RAPA group(P=0.077,P=0.127).The protein expression of Beclin-1 in MC3T3-E1 cells was lower in alcoholic group compared to RAPA group and alcoholic-RAPA group,and was higher in RAPA group compared to alcoholic-RAPA group(P=0.000,P=0.000; P=0.004).Conclusion:Cell autophagy may play a very important protective role in the pathological process of alcohol-induced ONFH.The decreased autophagy level caused by alcohol stimulation may be one of the reasons for causing ONFH,and its mechanism of action may be related to the influence of alcohol stimulation on the expression of key regulatory molecules(mTOR and Beclin1)in autophagy.

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備注/Memo

備注/Memo:
基金項(xiàng)目:浙江省醫(yī)藥衛(wèi)生科技計(jì)劃項(xiàng)目(2014KYB160)
通訊作者:孫遼軍 E-mail:[email protected]
更新日期/Last Update: 2019-02-25