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[1]趙永勝,李發(fā)東,楊新成,等.雪蓮強筋壯骨方對膝骨關(guān)節(jié)炎軟骨細胞p38絲裂原活化蛋白激酶信號傳導(dǎo)通路的影響[J].中醫(yī)正骨,2018,30(07):1-5.
　ZHAO Yongsheng,LI Fadong,YANG Xincheng,et al.Effect of Xuelian Qiangjin Zhuanggu Fang(雪蓮強筋壯骨方)on p38 mitogen-activated protein kinase signaling transduction pathway in chondrocytes of patients with knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2018,30(07):1-5.
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雪蓮強筋壯骨方對膝骨關(guān)節(jié)炎軟骨細胞p38絲裂原活化蛋白激酶信號傳導(dǎo)通路的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第30卷
期數(shù):
2018年07期
頁碼:
1-5
欄目:
基礎(chǔ)研究
出版日期:
2018-07-20

文章信息/Info

Title:
Effect of Xuelian Qiangjin Zhuanggu Fang(雪蓮強筋壯骨方)on p38 mitogen-activated protein kinase signaling transduction pathway in chondrocytes of patients with knee osteoarthritis
作者:
趙永勝; 李發(fā)東; 楊新成; 孟剛; 郭鑫; 趙輝; 潘有龍; 王曉樂
(新疆維吾爾自治區(qū)昌吉回族自治州中醫(yī)醫(yī)院,新疆 昌吉 831100)
Author(s):
ZHAO Yongsheng; LI Fadong; YANG Xincheng; MENG Gang; GUO Xin; ZHAO Hui; PAN Youlong; WANG Xiaole
Changji Hospital of Traditional Chinese Medicine,Changji 831100,Xinjiang,China
關(guān)鍵詞:
骨關(guān)節(jié)炎;  雪蓮強筋壯骨方;  軟骨細胞;  p38絲裂原活化蛋白激酶類;  信號傳導(dǎo)
Keywords:
osteoarthritis;  Xuelian Qiangjin Zhuanggu Fang;  chondrocytes;  p38 mitogen-activated protein kinases;  signal transduction
摘要:
目的:探討雪蓮強筋壯骨方對膝骨關(guān)節(jié)炎(knee osteoarthritis,KOA)軟骨細胞p38絲裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信號傳導(dǎo)通路的影響。方法:將24只3月齡雄性新西蘭大白兔隨機分為空白組、模型組、p38阻斷劑組及含藥血清組,每組6只。除空白組外,其余各組均采用改良Hulth法行KOA造模。造模成功后含藥血清組以雪蓮強筋壯骨方水煎劑灌胃(10 g·kg-1),其余各組均以等量生理鹽水灌胃,每天2次,連續(xù)干預(yù)7 d后靜脈采血,分別制成10%含藥血清和10%空白血清的DMEM培養(yǎng)液保存?zhèn)溆谩Ｑ逯苽渫瓿珊筇幩绖游?分離膝關(guān)節(jié)軟骨進行軟骨細胞培養(yǎng)。空白組和模型組以空白血清進行干預(yù),p38阻斷劑組以空白血清和SB203580進行干預(yù),含藥血清組以含藥血清進行干預(yù)。血清干預(yù)結(jié)束后,分別采用免疫熒光技術(shù)和蛋白免疫印跡法檢測各組軟骨細胞磷酸化p38MAPK水平。結(jié)果:免疫熒光檢測結(jié)果顯示,模型組磷酸化p38MAPK表達呈強陽性,p38阻斷劑組和含藥血清組次之,空白組最弱。蛋白免疫印跡檢測結(jié)果顯示,空白組磷酸化p38MAPK表達最弱,模型組磷酸化p38MAPK表達最強,p38阻斷劑組和含藥血清組磷酸化p38MAPK表達水平相當(dāng),介于空白組和模型組之間。經(jīng)掃描定量檢測,空白組、模型組、p38阻斷劑組和含藥血清組條帶的光密度值分別為0.31±0.06、0.83±0.11、0.58±0.45、0.55±0.56,差異有統(tǒng)計學(xué)意義(F=3.872,P=0.034)。空白組的光密度值小于模型組、p38阻斷劑組及含藥血清組(P=0.007,P=0.031,P=0.038); 模型組的光密度值大于p38阻斷劑組和含藥血清組(P=0.038,P=0.028); p38阻斷劑組和含藥血清組的光密度值比較,差異無統(tǒng)計學(xué)意義(P=0.066)。結(jié)論:雪蓮強筋壯骨方能阻滯KOA軟骨細胞p38MAPK信號傳導(dǎo)通路,其效果與p38MAPK阻斷劑SB203580相當(dāng)。
Abstract:
Objective:To explore the effects of Xuelian Qiangjin Zhuanggu Fang(雪蓮強筋壯骨方,XLQJZGF)on p38 mitogen-activated protein kinase(MAPK)signaling transduction pathway in chondrocytes of patients with knee osteoarthritis(KOA).Methods:Twenty four three-month-old male New Zealand rabbits were randomly divided into blank group,model group,p38 blocker group and medicated serum group,6 cases in each group.The KOA rabbit models were created in model group,p38 blocker group and medicated serum group by using modified Hulth method.After successful modeling,the rabbits in medicated serum group were intragastric administrated with XLQJZGF decoction(10 g/kg),while the rabbits in blank group,model group and p38 blocker group were intragastric administrated with isodose normal saline,twice a day for consecutive 7 days.Then their blood were fetched out from veins for making DMEM culture fluid added with 10% medicated serum and DMEM culture fluid added with 10% blank serum respectively for future use.After successful serum preparation,the rabbits were executed,and their chondrocytes were isolated from knee articular cartilages and were cultured in vitro.The chondrocytes were intervened with blank serum(blank group and model group),blank serum and SB203580(p38 blocker group)and medicated serum(medicated serum group)respectively.After the end of serum intervention,the levels of phosphorylated-p38 MAPK in chondrocytes were detected by using immunofluorescence technique and Western blot assay respectively.Results:The results of immunofluorescence assay showed that the phosphorylated-p38 MAPK exhibited high-to-low expression in model group,p38 blocker group and medicated serum group and blank group in turn.The results of Western blot assay showed that the phosphorylated-p38 MAPK exhibited low-to-high expression in blank group,p38 blocker group and medicated serum group and model group.The scanning and quantitative detection results showed that there was statistical difference in optical density values of strips between blank group,model group,p38 blocker group and medicated serum group(0.31+/-0.06,0.83+/-0.11,0.58+/-0.45,0.55+/-0.56; F=3.872,P=0.034).The optical density values were smaller in blank group compared to model group,p38 blocker group and medicated serum group(P=0.007,P=0.031,P=0.038),and were larger in model group compared to p38 blocker group and medicated serum group(P=0.038,P=0.028),and there was no statistical differences in optical density values between p38 blocker group and medicated serum group(P=0.066).Conclusion:XLQJZGF can block the p38 MAPK signaling transduction pathway in chondrocytes in patients with KOA,and it is similar to SB203580(p38 MAPK blocker)in curative effects.

參考文獻/References:


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備注/Memo

備注/Memo:
基金項目:新疆維吾爾自治區(qū)衛(wèi)生計生委青年科技人才專項科研項目(2015Y08) 通訊作者:李發(fā)東 E-mail:[email protected]
更新日期/Last Update: 2018-11-30