84年鼠女哪年财运最旺,857comvvv色九欧美激情|85PO_87国产精品欲av国产av资源

[1]蔡立雄,霍志謙,孫丙銀,等.改良兔股骨骨缺損并骨延長模型的建立[J].中醫(yī)正骨,2018,30(01):25-28.
　CAI Lixiong,HUO Zhiqian,SUN Bingyin,et al.Establishment of improved rabbit model of femoral defect and lengthening[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2018,30(01):25-28.
點擊復制

改良兔股骨骨缺損并骨延長模型的建立()
分享到：

《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第30卷
期數(shù):
2018年01期
頁碼:
25-28
欄目:
基礎(chǔ)研究
出版日期:
2018-01-20

文章信息/Info

Title:
Establishment of improved rabbit model of femoral defect and lengthening
作者:
蔡立雄1; 霍志謙1; 孫丙銀2; 吳峰1; 溫建強1; 鄭揚康1; 楊海韻1
1.廣東省佛山市中醫(yī)院,廣東 佛山 528000; 2.佛山健翔醫(yī)院,廣東 佛山 528000
Author(s):
CAI Lixiong1; HUO Zhiqian1; SUN Bingyin2; WU Feng1; WEN Jianqiang1; ZHENG Yangkang1; YANG Haiyun1
1.Foshan Hospital of Traditional Chinese Medicine,Foshan 528000,Guangdong,China 2.Foshan Jianxiang Hospital,Foshan 528000,Guangdong,China
關(guān)鍵詞:
骨膜;  骨延長術(shù);  骨缺損;  兔;  模型; 動物
Keywords:
Keywords periosteum;  bone lengthening;  bone defect;  rabbits;  models; animal
摘要:
目的:探討建立兔股骨骨缺損并骨延長模型的有效方法。方法:將36只3～4月齡健康新西蘭白兔隨機分為實驗組和對照組。將自制骨延長牽張器安裝于2組動物左側(cè)股骨上后,于股骨中段截骨,做長約12 mm的骨缺損,其中實驗組截骨時保護股骨外側(cè)骨膜的連續(xù)性,對照組行全層截骨而未保護骨膜。靜息期1周后開始進行骨延長。于骨延長結(jié)束后第28天,攝股骨X線片,觀察骨痂生長情況,并取股骨標本進行肉眼大體觀察。結(jié)果:①股骨標本X線檢查結(jié)果。實驗組股骨干延長區(qū)域呈高密度、均勻影像,新生骨質(zhì)區(qū)與兩斷端邊界模糊,外層皮質(zhì)連續(xù),可見大量新生骨痂生長; 對照組股骨干延長區(qū)域呈較高密度、不均勻影像,新生骨質(zhì)區(qū)與股骨近端骨干連接部分邊界模糊,與股骨遠端骨干連接部分邊界較清,尚存臺階,外層皮質(zhì)尚未完全連續(xù),可見新生骨痂生長。②股骨標本肉眼觀察結(jié)果。實驗組骨延長區(qū)域骨質(zhì)連續(xù),連接完整,骨痂量多,新生骨兩端界線模糊; 對照組骨延長區(qū)域骨質(zhì)相對連續(xù),連接相對完整,見骨痂生長,新生骨遠端尚有部分缺損,未完全填充骨缺損區(qū)。結(jié)論:在股骨截骨過程中保留部分骨膜,可以更有效地建立兔股骨骨缺損并骨延長模型。
Abstract:
ABSTRACT Objective:To explore the effective method of building rabbit model of femoral defect and lengthening.Methods:Thirty-six 3-4-month-old healthy New Zealand white rabbits were randomly divided into experimental group and control group.The self-made bone lengthening-stretching device was fixed in the left femur of the rabbit,and osteotomy of middle femur was performed to make 12 mm long bone defect.The continuity of femoral lateral periosteum was protected when osteotomy was performed in experimental group,while the periosteum was not protected and full-thickness osteotomy was performed in control group.The bone lengthening surgery was carried out after one-week rest period.At the 28th day after the end of bone lengthening,the femoral X-ray films were taken,and the bony callus growth was observed,and then macroscopic observation was performed on the specimens of femurs.Results:The X-ray examination results of femur specimens demonstrated that in experimental group the bone lengthening area of femoral shaft presented with high density and homogeneous imaging,and the boundary between newly formed bone region and the two broken ends was fuzzy,meanwhile,the continuous outer cortex and a large number of newly formed bony calluses were found; while in control group the bone lengthening area of femoral shaft presented with high density and inhomogeneous imaging,and the boundary between newly formed bone region and the proximal femoral shaft was fuzzy,and the boundary between newly formed bone region and the distal femoral shaft was clear,meanwhile,the discontinuous outer cortex and some newly formed bony calluses were found.The results of macroscopic observation on femur specimens demonstrated that continuous bone tissues and a large number of bony calluses were found in the bone lengthening area of femoral shaft in experimental group,and the two ends of the newly formed bone were fuzzy; while partly continuous bone tissues and some bony calluses were found in the bone lengthening area of femoral shaft in control group,and partial bone defects could be found in the distal end of newly formed bone.Conclusion:The femoral defect and lengthening rabbit model can be built more effectively by preserving some periosteum in the process of the femoral osteotomy.

參考文獻/References:

[1] 陰彥林.自體骨髓基質(zhì)細胞與紅骨髓修復保留骨膜骨缺損的比較研究[D].石家莊:河北醫(yī)科大學,2005.
[2] 劉昌戎,沈為棟,彭偉,等.保留骨膜的兔橈骨缺損性骨不連動物模型的制備[J].海南醫(yī)學院學報,2013,19(6):725-727.
[3] 謝利娜,潘巨利,黃欣.骨膜原位成骨促進兔下頜骨缺損修復的研究[J].北京口腔醫(yī)學,2009,17(6):110-112.
[4] 孫丙銀,朱潔英,熊建尉,等.牽張成骨模型兔的建立與評估[J].中國組織工程研究,2017,21(16):2576-2581.
[5] 黃楓,孫丙銀,蔡立雄,等.兔股骨牽張器的研制及其在實驗研究中的應用[J].中國中醫(yī)骨傷科雜志,2013,21(8):5-9.
[6] 蔡立雄.骨延長臨床應用與淫羊藿苷促進牽拉新骨形成的實驗研究[D].廣東:廣州中醫(yī)藥大學,2014.
[7] 詹玉林,侯國柱,安智全,等.兔骨缺損模型中骨膜對骨形態(tài)發(fā)生蛋白-2分泌量及骨愈合影響的實驗研究[J].中華創(chuàng)傷骨科雜志,2013,15(10):884-888.
[8] 任志勇,李濤,張維彬,等.兔脛骨干骺端骨膜外截骨對延長區(qū)成骨作用的影響[J].中國矯形外科雜志,2015,23(2):150-155.
[9] 韋露,羅高斌,薄占東,等.幼兔腓骨切除后再生腓骨的功能研究[J].重慶醫(yī)學,2015,43(24):3337-3340.
[10] ZHAO L,ZHAO JL,YU JJ,et al.In vivo investigation of tissue-engineered periosteum for the repair of allogeneic critical size bone defects in rabbits[J].Regen Med,2017,12(4):353-364.
[11] CHASSANIDIS CG,MALIZOS KN,VARITIMIDIS S,et al.Smoking affects mRNA expression of bone morphogenetic proteins in human periosteum[J].J Bone Joint Surg Bri,2012,94(10):1427-1432.
[12] 王永剛,裴國獻,張洪濤,等.兔股骨干缺損模型的制備及在組織工程骨實驗中的應用[J].中華創(chuàng)傷骨科雜志,2005,7(10):971-974.
[13] 鮑小剛,許國華.兔脛骨大段完全骨缺損的解剖學模型構(gòu)建[J].中國組織工程研究,2016,20(24):3508-3515.
[14] 沈為棟,劉昌戎,張鐵洪,等.新西蘭大白兔橈骨的解剖學觀測[J].中華實驗外科雜志,2010,27(9):1331-1332.
[15] 馬金玲,潘巨利,王東勝.自發(fā)成骨修復小型豬下頜骨缺損的組織病理學研究[J].北京口腔醫(yī)學,2008,16(6):304-308.

相似文獻/References:

[1]韋健,容安,周建飛,等.腓骨延長術(shù)治療外踝開放性骨缺損[J].中醫(yī)正骨,2016,28(10):62.
[2]趙仁歡,盧賓,劉昕,等.尺骨截骨Orthofix外固定支架延長術(shù)治療兒童尺骨干骺端續(xù)連癥[J].中醫(yī)正骨,2017,29(01):56.
[3]劉江濤,萬春友,張濤,等.骨搬移術(shù)結(jié)合貫通沖洗引流治療創(chuàng)傷性骨髓炎[J].中醫(yī)正骨,2017,29(02):47.
[4]靳國強,韓宗昌,趙蕾,等.環(huán)形外固定架在膝關(guān)節(jié)融合術(shù)治療膝關(guān)節(jié)感染中的應用[J].中醫(yī)正骨,2019,31(10):49.
[5]呂秉舒,田亞敏,李東升,等.帶血管腓骨翻轉(zhuǎn)內(nèi)固定聯(lián)合脛骨微創(chuàng)截骨Ilizarov骨延長術(shù)治療先天性脛骨假關(guān)節(jié)[J].中醫(yī)正骨,2020,32(06):65.

備注/Memo

備注/Memo:
基金項目:廣東省佛山市科學技術(shù)局科研項目(2015AB00350) 通訊作者:楊海韻 E-mail:[email protected]
更新日期/Last Update: 2018-06-02