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[1]查朱青,趙治偉.Ⅰ型牛膠原蛋白生物膜防止肌腱修復(fù)術(shù)后黏連的實驗研究[J].中醫(yī)正骨,2017,29(09):23-27.
　ZHA Zhuqing,ZHAO Zhiwei.An experimental study of bovine typeⅠcollagen protein biofilms for prevention of tendon adhesions after tendon repair surgery[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2017,29(09):23-27.
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Ⅰ型牛膠原蛋白生物膜防止肌腱修復(fù)術(shù)后黏連的實驗研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第29卷
期數(shù):
2017年09期
頁碼:
23-27
欄目:
基礎(chǔ)研究
出版日期:
2017-09-20

文章信息/Info

Title:
An experimental study of bovine typeⅠcollagen protein biofilms for prevention of tendon adhesions after tendon repair surgery
作者:
查朱青; 趙治偉
河南省洛陽正骨醫(yī)院/河南省骨科醫(yī)院,河南 鄭州 450016
Author(s):
ZHA Zhuqing; ZHAO Zhiwei
Luoyang Orthopedic-Traumatological Hospital,Zhengzhou 450016,Henan,China
關(guān)鍵詞:
組織黏連;  肌腱;  生物膜;  膠原Ⅰ型;  動物實驗
Keywords:
Key words tissue adhesions;  tendon;  biofilms;  collagen typeⅠ;  animal experimentation
摘要:
目的:觀察Ⅰ型牛膠原蛋白生物膜防止肌腱修復(fù)術(shù)后黏連的效果,并探討其可能的作用機理。方法:將60只3月齡CV級三黃雞隨機分為假手術(shù)組、模型組和生物膜組,每組20只。手術(shù)暴露所有三黃雞右足第3趾趾深屈肌肌腱,暴露肌腱后假手術(shù)組直接關(guān)閉切口,模型組和生物膜組采用第3趾趾深屈肌肌腱斷裂1/2再吻合建立肌腱斷裂修復(fù)模型。肌腱修復(fù)后模型組直接關(guān)閉切口,生物膜組在修復(fù)的肌腱外側(cè)包繞Ⅰ型牛膠原蛋白生物膜后關(guān)閉切口。觀察實驗期間各組動物健康狀況、切口愈合情況及是否發(fā)生肌腱斷裂。造模后第2、4、8周分別從各組選取三黃雞,觀察肌腱組織病理變化,測定肌腱滑動距離和最大抗斷裂載荷。結(jié)果:實驗期間各組均無動物死亡,切口均愈合良好,未發(fā)生肌腱斷裂。肌腱組織病理學(xué)觀察結(jié)果顯示,與模型組相比,生物膜組肌腱斷端成纖維細胞及膠原纖維數(shù)量明顯增加,肌腱吻合端外周成纖維細胞聚集和炎性細胞浸潤明顯減少。造模后第2周時,3組三黃雞第3趾趾深屈肌肌腱在1 N時的滑動距離比較,差異有統(tǒng)計學(xué)意義[(0.92±0.02)cm,(0.61±0.03)cm,(0.86±0.01)cm,F=196.200,P=0.000]。模型組和生物膜組的肌腱滑動距離均小于假手術(shù)組(P=0.000; P=0.017),生物膜組的肌腱滑動距離大于模型組(P=0.001)。造模后第4周時,3組三黃雞第3趾趾深屈肌肌腱在1 N時的滑動距離比較,差異有統(tǒng)計學(xué)意義[(0.91±0.01)cm,(0.59±0.01)cm,(0.87±0.05)cm,F=113.977,P=0.000]。模型組的肌腱滑動距離小于假手術(shù)組和生物膜組(P=0.000; P=0.000); 生物膜組與假手術(shù)組的肌腱滑動距離比較,差異無統(tǒng)計學(xué)意義(P=3.651)。造模后第8周時,3組三黃雞第3趾趾深屈肌肌腱在1 N時的滑動距離比較,差異有統(tǒng)計學(xué)意義[(0.91±0.05)cm,(0.58±0.01)cm,(0.90±0.04)cm,F=114.848,P=0.000]。模型組的肌腱滑動距離小于假手術(shù)組和生物膜組(P=0.000; P=0.000); 生物膜組與假手術(shù)組的肌腱滑動距離比較,差異無統(tǒng)計學(xué)意義(P=4.732)。造模后第2周時,3組三黃雞第3趾趾深屈肌肌腱最大抗斷裂載荷比較,差異有統(tǒng)計學(xué)意義[(0.99±0.01)N,(0.33±0.01)N,(0.38±0.02)N,F=2 052.221,P=0.000]。模型組的最大抗斷裂載荷小于假手術(shù)組和生物膜組(P=0.000; P=0.031); 假手術(shù)組的最大抗斷裂載荷大于模型組(P=0.000)。造模后第4周時,3組三黃雞第3趾趾深屈肌肌腱最大抗斷裂載荷比較,差異有統(tǒng)計學(xué)意義[(0.99±0.01)N,(0.78±0.01)N,(0.81±0.02)N,F=300.318,P=0.000]。模型組的最大抗斷裂載荷小于假手術(shù)組和生物膜組(P=0.000; P=0.017); 假手術(shù)組的最大抗斷裂載荷大于生物膜組(P=0.000)。造模后第8周時,3組三黃雞第3趾趾深屈肌肌腱最大抗斷裂載荷比較,差異有統(tǒng)計學(xué)意義[(0.99±0.01)N,(0.96±0.02)N,(0.97±0.01)N,F=6.065,P=0.021]。模型組的最大抗斷裂載荷小于假手術(shù)組(P=0.035); 生物膜組的最大抗斷裂載荷與假手術(shù)組、模型組比較,差異均無統(tǒng)計學(xué)意義(P=3.513; P=7.582)。結(jié)論:Ⅰ型牛膠原蛋白生物膜可以通過促進內(nèi)源性愈合、減少外源性愈合防止肌腱修復(fù)術(shù)后黏連。
Abstract:
ABSTRACT Objective:To observe the effect of bovine typeⅠcollagen protein biofilms in prevention of tendon adhesions after tendon repair surgery and to explore its possible mechanism of action.Methods:Sixty 3-month-old CV-grade three-yellow chickens were randomly divided into sham-operated group,model group and biofilm group,20 cases in each group.The surgery was performed on the 3rd toe of right foot of all chickens to expose the digitorum profundus flexor tendon(DPFT).After exposure of the tendon,the incisions were closed directly in sham-operated group and the tendon rupture-and-repair models were built in model group and biofilm group through rupture and reanastomosis of half of the DPFT of the 3rd toe.The incisions were closed directly after tendon repairing in model group,while the incisions were closed after wrapping the bovine typeⅠcollagen protein biofilms around the repaired tendons in biofilm group.The health status,incision healing and tendon rupture were observed and compared between the 3 groups during the experiment.Some three-yellow chickens were selected from each group at 2,4 and 8 weeks after modeling.The pathological changes of tendon tissues were observed and the sliding distance and maximum anti-rupture load of tendons were measured.Results:All incisions healed well and no death and tendon rupture were found in the 3 groups.The results of histopathological observation on tendon tissues showed that fibroblasts and collagenous fiber increased more obviously in broken ends of tendons while fibroblasts aggregation and inflammatory cells infiltration decreased more obviously in anastomosis ends of tendons in biofilm group compared to model group.There was statistical difference in the sliding distance of DPFT of the 3rd toe at 1 N between the 3 groups at 2 weeks after modeling(0.92+/-0.02,0.61+/-0.03,0.86+/-0.01 cm,F=196.200,P=0.000).The tendon sliding distances were less in model group and biofilm group compared to sham-operated group(P=0.000; P=0.017)and were greater in biofilm group compared to model group(P=0.001).There was statistical difference in the sliding distance of DPFT of the 3rd toe at 1 N between the 3 groups at 4 weeks after modeling(0.91+/-0.01,0.59+/-0.01,0.87+/-0.05 cm,F=113.977,P=0.000).The tendon sliding distances were less in model group compared to sham-operated group and biofilm group(P=0.000; P=0.000),and there was no statistical difference in the sliding distance between biofilm group and sham-operated group(P=3.651).There was statistical difference in the sliding distance of DPFT of the 3rd toe at 1 N between the 3 groups at 8 weeks after modeling(0.91+/-0.05,0.58+/-0.01,0.90+/-0.04 cm,F=114.848,P=0.000).The tendon sliding distances were less in model group compared to sham-operated group and biofilm group(P=0.000; P=0.000),and there was no statistical difference in the sliding distance between biofilm group and sham-operated group(P=4.732).There was statistical difference in the maximum anti-rupture loads of DPFT of the 3rd toe between the 3 groups at 2 weeks after modeling(0.99+/-0.01,0.33+/-0.01,0.38+/-0.02 N,F=2 052.221,P=0.000).The maximum anti-rupture loads were less in model group compared to sham-operated group and biofilm group(P=0.000; P=0.031),and the maximum anti-rupture loads were greater in sham-operated group compared to model group(P=0.000).There was statistical difference in the maximum anti-rupture loads of DPFT of the 3rd toe between the 3 groups at 4 weeks after modeling(0.99+/-0.01,0.78+/-0.01,0.81+/-0.02 N,F=300.318,P=0.000).The maximum anti-rupture loads were less in model group compared to sham-operated group and biofilm group(P=0.000; P=0.017),and the maximum anti-rupture loads were greater in sham-operated group compared to biofilm group(P=0.000).There was statistical difference in the maximum anti-rupture load of DPFT of the 3rd toe between the 3 groups at 8 weeks after modeling(0.99+/-0.01,0.96+/-0.02,0.97+/-0.01 N,F=6.065,P=0.021).The maximum anti-rupture loads were less in model group compared to sham-operated group(P=0.035),and there was no statistical difference in the maximum anti-rupture loads between biofilm group and sham-operated group and between biofilm group and model group(P=3.513; P=7.582).Conclusion:Bovine TypeⅠcollagen protein biofilms can prevent tendon adhesions after tendon repair surgery through promoting endogenous healing and reducing exogenous healing.

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通訊作者:查朱青 E-mail:[email protected]
更新日期/Last Update: 2018-02-23