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[1]孫龍?zhí)?姚華海,王曉洛,等.強(qiáng)骨飲對假體周圍骨溶解模型大鼠骨密度的影響及其作用機(jī)制研究[J].中醫(yī)正骨,2017,29(09):19-22.
　SUN Longtai,YAO Huahai,WANG Xiaoluo,et al.Effect of Qianggu Yin(強(qiáng)骨飲)on bone density of peri-prosthetic osteolysis rat models and its mechanism of action[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2017,29(09):19-22.
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強(qiáng)骨飲對假體周圍骨溶解模型大鼠骨密度的影響及其作用機(jī)制研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第29卷
期數(shù):
2017年09期
頁碼:
19-22
欄目:
基礎(chǔ)研究
出版日期:
2017-09-20

文章信息/Info

Title:
Effect of Qianggu Yin(強(qiáng)骨飲)on bone density of peri-prosthetic osteolysis rat models and its mechanism of action
作者:
姚華海1; 王曉洛1; 崔龍慷1; 吳連國2
1.浙江中醫(yī)藥大學(xué),浙江 杭州 310053; 2.浙江中醫(yī)藥大學(xué)附屬第二醫(yī)院,浙江 杭州 310005
Author(s):
SUN Longtai1; YAO Huahai1; WANG Xiaoluo1; CUI Longkang1; WU Lianguo2
1.Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China 2.The Second Affiliated Hospital of Zhengjiang Chinese Medical University,Hangzhou 310005,Zhejiang,China
關(guān)鍵詞:
關(guān)節(jié)成形術(shù); 置換;  強(qiáng)骨飲;  骨質(zhì)溶解;  假體失效;  骨密度;  大鼠; Sprague-Dawley;  動物實(shí)驗(yàn)
Keywords:
Key words arthroplasty; replacement;  Qianggu Yin;  osteolysis;  prosthesis failure;  bone density;  rats; Sprague-Dawley;  animal experimentation
摘要:
目的:探討強(qiáng)骨飲對假體周圍骨溶解模型大鼠骨密度的影響及其作用機(jī)制。方法:選取30只成年雄性SD大鼠,隨機(jī)分為空白組、模型組和強(qiáng)骨飲組,每組10只。空白組不進(jìn)行造模; 模型組和強(qiáng)骨飲組大鼠右側(cè)股骨植入高密度聚乙烯顆粒和鈦棒,制備聚乙烯顆粒誘導(dǎo)的假體周圍骨溶解模型。造模手術(shù)3 d后,強(qiáng)骨飲組大鼠以制備好的強(qiáng)骨飲藥液灌胃,每周3次,連續(xù)給藥11周。空白組和模型組均不進(jìn)行藥物干預(yù)。造模后12周,取大鼠靜脈血用ELISA法測定血清抗酒石酸酸性磷酸酶5b(tartrate resistant acid phosphatase 5b,TRACP5b)水平,取右側(cè)股骨進(jìn)行骨密度檢測,并以ELISA法測定股骨組織中腫瘤壞死因子-α(tumor necrosis factor α,TNF-α)水平。結(jié)果:實(shí)驗(yàn)過程中各組大鼠日常行為無差別,精神狀況良好,反應(yīng)靈敏,體質(zhì)量逐漸增加。模型組和強(qiáng)骨飲組大鼠術(shù)后1周切口愈合。實(shí)驗(yàn)全過程中各組均無大鼠死亡。3組大鼠血清TRACP5b水平比較,差異有統(tǒng)計(jì)學(xué)意義[(1.83±0.42)U·L-1,(2.40±0.43)U·L-1,(1.90±0.28)U·L-1,F=9.323,P=0.002]。模型組血清TRACP5b水平高于空白組和強(qiáng)骨飲組(P=0.001; P=0.002)。3組大鼠股骨骨密度比較,差異有統(tǒng)計(jì)學(xué)意義[(0.162±0.009)g·cm-2,(0.119±0.010)g·cm-2,(0.140±0.007)g·cm-2,F=74.323,P=0.000]。模型組股骨骨密度低于空白組和強(qiáng)骨飲組(P=0.000; P=0.000)。3組大鼠股骨組織中TNF-α水平比較,差異有統(tǒng)計(jì)學(xué)意義[(70.21±8.83)pg·mL-1,(175.32±14.95)pg·mL-1,(129.73±12.86)pg·mL-1,F=160.242,P=0.000]。模型組股骨組織TNF-α水平高于空白組和強(qiáng)骨飲組(P=0.000; P=0.000)。結(jié)論:強(qiáng)骨飲可以增加假體周圍骨溶解模型大鼠的骨密度,其機(jī)制可能是通過減輕炎癥反應(yīng),抑制破骨細(xì)胞活性,從而減少假體周圍骨吸收。
Abstract:
ABSTRACT Objective:To explore the effects of Qianggu Yin(強(qiáng)骨飲,QGY)on bone density of peri-prosthetic osteolysis rat models and its mechanism of action.Methods:Thirty adult male SD rats were selected and randomly divided into blank group,model group and QGY group,10 cases in each group.High density polyethylene(HDPE)particles and titanium rods were implanted into the right femurs of rats in model group and QGY group to build the peri-prosthetic osteolysis models induced by HDPE particles.At 3 days after the molding operation,the rats in QGY group were intragastric administrated with prepared QGY,3 times a week for consecutive 11 weeks.No drug intervention were performed on rats in blank group and model group.At 12 weeks after the modeling,the venous blood were fetched out and the serum levels of tartrate resistant acid phosphatase 5b(TRACP5b)were measured by using ELISA.The right femurs were taken from rats for detecting the bone densities,and the levels of tumor necrosis factor α(TNF-α)in femoral tissues were measured by using ELISA.Results:There were no difference in the daily actions of rats between the 3 groups during the course of experiment.The rats were in good mental states and rapid response and their body masses gradually increased.The incisions of rats in model group and QGY group were healed at 1 week after the surgery.No rats died in each group during the experiment.There was statistical difference in the serum levels of TRACP5b between the 3 groups(1.83+/-0.42,2.40+/-0.43,1.90+/-0.28 U/L,F=9.323,P=0.002).The serum levels of TRACP5b were higher in model group compared to blank group and QGY group(P=0.001; P=0.002).There was statistical difference in the femoral bone density between the 3 groups(0.162+/-0.009,0.119+/-0.010,0.140+/-0.007 g/cm(2),F=74.323,P=0.000).The femoral bone densities were lower in model group compared to blank group and QGY group(P=0.000; P=0.000).There was statistical difference in the TNF-α levels in femoral tissues between the 3 groups(70.21+/-8.83,175.32+/-14.95,129.73+/-12.86 pg/ml,F=160.242,P=0.000).The TNF-α levels in femoral tissues were higher in model group compared to blank group and QGY group(P=0.000; P=0.000).Conclusion:QGY can alleviate the inflammatory reaction and inhibit the cytoactive of osteoclasts and accordingly reduce peri-prosthetic bone resorption,which may be its mechanisms of action for increasing bone density of peri-prosthetic osteolysis rat models.

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備注/Memo

備注/Memo:
基金項(xiàng)目:浙江省一流學(xué)科(B類)建設(shè)項(xiàng)目; 浙江省科技廳公益技術(shù)應(yīng)用研究計(jì)劃項(xiàng)目(2016C33128); 教育部高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金聯(lián)合資助課題(20133322120004) 通訊作者:吳連國 E-mail:[email protected]
更新日期/Last Update: 2018-02-23