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[1]鄭文偉,何曉娟,賈良良,等.跳骨片含藥血清抑制脂多糖誘導的軟骨細胞炎癥反應的作用機制研究[J].中醫(yī)正骨,2017,29(08):8-16.
　ZHENG Wenwei,HE Xiaojuan,JIA Liangliang,et al.Study on mechanism of action of Tiaogu Pian(跳骨片)medicated serum in inhibiting inflammatory reaction induced by lipopolysaccharides in chondrocytes[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2017,29(08):8-16.
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跳骨片含藥血清抑制脂多糖誘導的軟骨細胞炎癥反應的作用機制研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第29卷
期數(shù):
2017年08期
頁碼:
8-16
欄目:
基礎研究
出版日期:
2017-08-20

文章信息/Info

Title:
Study on mechanism of action of Tiaogu Pian(跳骨片)medicated serum in inhibiting inflammatory reaction induced by lipopolysaccharides in chondrocytes
作者:
鄭文偉1; 何曉娟1; 賈良良1; 馬玉環(huán)1; 陳后煌1; 邵翔1; 陳達1; 劉獻祥1; 李西海1; 葉蕻芝2
1.福建中醫(yī)藥大學,福建 福州 350122; 2.福建省中西醫(yī)結合老年性疾病重點實驗室,福建 福州 350122
Author(s):
ZHENG Wenwei1; HE Xiaojuan1; JIA Liangliang1; MA Yuhuan1; CHEN Houhuang1; SHAO Xiang1; CHEN Da1; LIU Xianxiang2; LI Xihai2; YE Hongzhi1
1.Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China 2.Fujian Key Laboratory of Integrated Medicine on Geriatrics,Fuzhou 350122,Fujian,China
關鍵詞:
骨關節(jié)炎;  軟骨細胞;  跳骨片;  軟骨退變;  Wnt/β-catenin信號通路;  脂多糖類;  基質金屬蛋白酶類;  炎癥反應;  動物實驗
Keywords:
Key words osteoarthritis;  chondrocytes;  Tiaogu Pian;  cartilage degeneration;  Wnt/β-catenin signaling pathway;  lipopolysaccharides;  matrix metalloproteinases;  inflammatory reaction;  animal experimentation
摘要:
目的:探討跳骨片含藥血清抑制脂多糖誘導的軟骨細胞炎癥反應的作用機制。方法:將10只8周齡雄性SD大鼠隨機分為跳骨片組和空白組,跳骨片組以0.32 g·kg-1劑量的跳骨片灌胃,空白組給予等量生理鹽水灌胃; 每天灌胃1次,連續(xù)7 d; 末次灌胃1 h后,經腹主動脈取血,分別制備跳骨片含藥血清和空白血清,低溫保存?zhèn)溆谩?0只4周齡SD大鼠膝關節(jié)軟骨中分離軟骨細胞并培養(yǎng),光學顯微鏡下觀察軟骨細胞形態(tài),并用Ⅱ型膠原酶免疫組化鑒定。將培養(yǎng)好的第2代軟骨細胞隨機分為空白血清組、模型組、跳骨片含藥血清組,其中空白血清組以含10%空白血清的培養(yǎng)基(dulbecco modified eagle medium,DMEM)培養(yǎng); 模型組以濃度為10 ng·mL-1的脂多糖和含10%空白血清的DMEM培養(yǎng); 跳骨片含藥血清組以濃度為10 ng·mL-1的脂多糖和含10%跳骨片含藥血清的DMEM培養(yǎng); 3組均連續(xù)干預培養(yǎng)8 h,采用酶聯(lián)免疫吸附法檢測軟骨細胞基質金屬蛋白酶(matrix metalloproteinase,MMP)3、MMP9含量,采用熒光定量RT-PCR法檢測軟骨細胞中Wnt/β-catenin信號通路相關基因表達水平,采用Western blot法檢測軟骨細胞中β-鏈蛋白(β-catenin)、卷曲蛋白-2(Frizzled-2)的蛋白表達量,采用免疫熒光檢測法檢測軟骨細胞中β-catenin、糖原合成酶激酶-3β(glycogen synthasc kinase-3β,GSK-3β)、蛋白多糖1(proteoglycans 1,PGS1)的蛋白表達量。結果:①軟骨細胞免疫組化鑒定結果。第2代軟骨細胞胞漿及細胞膜呈棕黃色陽性染色,具有典型的軟骨細胞生物學特征。②軟骨細胞MMP3、MMP9含量。脂多糖干預8 h后,空白血清組、模型組和跳骨片含藥血清組的軟骨細胞MMP3、MMP9含量比較,組間差異均有統(tǒng)計學意義[(34.019±1.036)ng·mL-1,(44.645±2.473)ng·mL-1,(32.941±1.792)ng·mL-1,F=36.060,P=0.000;(1.348±0.038)ng·mL-1,(1.562±0.112)ng·mL-1,(1.331±0.015)ng·mL-1,F=11.319,P=0.000]; 模型組軟骨細胞MMP3、MMP9含量均高于空白血清組(LSD-t=-7.016,P=0.000; LSD-t=-3.768,P=0.003); 跳骨片含藥血清組軟骨細胞MMP3、MMP9含量均低于模型組(LSD-t=7.652,P=0.000; LSD-t=4.066,P=0.002); 空白血清組軟骨細胞MMP3、MMP9含量與跳骨片含藥血清組比較,差異均無統(tǒng)計意義(LSD-t=0.635,P=0.549; LSD-t=0.299,P=0.770)。③軟骨細胞中Wnt/β-catenin信號通路相關基因的表達。脂多糖干預8 h后,空白血清組、模型組和跳骨片含藥血清組軟骨細胞中β-catenin、GSK-3β、Frizzled-2、Wnt-4、CKI-ε基因表達量比較,組間差異均有統(tǒng)計學意義(1.000±0.275,2.258±0.206,1.431±0.304,F=36.709,P=0.000; 1.000±0.133,0.417±0.104,0.842±0.094,F=29.259,P=0.000; 1.000±0.191,1.737±0.238,1.445±0.337,F=7.027,P=0.015; 1.000±0.341,3.801±0.579,1.876±0.388,F=71.903,P=0.000; 1.000±0.309,2.208±0.708,1.441±0.421,F=64.178,P=0.000); 模型組軟骨細胞中β-catenin、Frizzled-2、Wnt-4、CKI-ε基因表達量均高于空白血清組(LSD-t=-8.431,P=0.000; LSD-t=-3.723,P=0.005; LSD-t=8.062,P=0.000; LSD-t=-11.235,P=0.000),GSK-3β基因表達量低于空白血清組(LSD-t=7.397,P=0.000); 跳骨片含藥血清組軟骨細胞中β-catenin、Frizzled-2、Wnt-4、CKI-ε基因表達量均低于模型組(LSD-t=5.541,P=0.000; LSD-t=1.477,P=0.017; LSD-t=8.062,P=0.000; LSD-t=6.882,P=0.000),GSK-3β基因表達量高于模型組(LSD-t=-5.387,P=0.000); 空白血清組軟骨細胞中β-catenin、Wnt-4、CKI-ε基因表達量均低于跳骨片含藥血清組(LSD-t=-2.289,P=0.018; LSD-t=-3.658,P=0.005; LSD-t=-4.352,P=0.002); 空白血清組軟骨細胞中GSK-3β、Frizzled-2基因表達量與跳骨片含藥血清組比較,差異均無統(tǒng)計學意義(LSD-t=2.009,P=0.075; LSD-t=-3.658,P=0.051)。④軟骨細胞中β-catenin、Frizzled-2的蛋白表達。脂多糖干預8 h后,空白血清組、模型組和跳骨片含藥血清組軟骨細胞中β-catenin、Frizzled-2蛋白表達量比較,組間差異均有統(tǒng)計學意義(0.449±0.063,0.746±0.156,0.549±0.056,F=5.323,P=0.026; 1.348±0.038,1.562±0.112,1.331±0.015,F=6.291,P=0.034); 模型組軟骨細胞中β-catenin、Frizzled-2蛋白表達量高于空白血清組(LSD-t=-11.235,P=0.005; LSD-t=-3.104,P=0.021); 跳骨片含藥血清組軟骨細胞中β-catenin、Frizzled-2蛋白表達量低于模型組(LSD-t=6.883,P=0.037; LSD-t=3.039,P=0.023); 空白----------------------------------------------- 血清組軟骨細胞中β-catenin蛋白表達量低于跳骨片含藥血清組(LSD-t=-4.352,P=0.002); 空白血清組軟骨細胞中Frizzled-2蛋白表達量與跳骨片含藥血清組比較,差異無統(tǒng)計學意義(LSD-t=-0.065,P=0.950)。⑤軟骨細胞中β-catenin、GSK-3β、PGS1的蛋白表達。脂多糖干預8 h后,軟骨細胞中β-catenin、GSK-3β、PGS1蛋白染色明顯,呈綠色; 空白血清組、模型組和跳骨片含藥血清組軟骨細胞中β-catenin、GSK-3β、PGS1蛋白表達量比較,組間差異均有統(tǒng)計學意義(0.014±0.002,0.029±0.006,0.018±0.002,F=9.910,P=0.013; 0.380±0.011,0.237±0.015,0.287±0.002,F=56.639,P=0.000; 0.034±0.003,0.022±0.002,0.029±0.003,F=27.232,P=0.001); 模型組軟骨細胞β-catenin蛋白表達量高于空白血清組(LSD-t=-4.103,P=0.006),GSK-3β、PGS1蛋白表達量低于空白血清組(LSD-t=1.048,P=0.000; t=7.365,P=0.000); 跳骨片含藥血清組軟骨細胞β-catenin蛋白表達量低于模型組(LSD-t=-3.548,P=0.012),GSK-3β、PGS1蛋白表達量高于模型組(LSD-t=-3.657,P=0.011; LSD-t=-3.273,P=0.017); 空白血清組軟骨細胞中β-catenin蛋白表達量與跳骨片含藥血清組比較,差異無統(tǒng)計學意義(LSD-t=-0.554,P=0.599); 空白血清組軟骨細胞中GSK-3β、PGS1蛋白表達量高于跳骨片含藥血清組(LSD-t=6.827,P=0.000; LSD-t=4.092,P=0.010)。結論:跳骨片含藥血清可以抑制脂多糖誘導的軟骨細胞炎癥反應,延緩關節(jié)軟骨退變。其作用機制可能與Wnt/β-catenin信號通路的調控有關,其中β-catenin、Frizzled-2、GSK-3β、Wnt-4、CKI-ε基因可能是該信號通路的重要靶點。但是由于引起OA的因素眾多且跳骨片含藥血清成分多且復雜,有待于進一步研究證實。
Abstract:
ABSTRACT Objective:To explore the mechanism of action of Tiaogu Pian(跳骨片,TGP)medicated serum in inhibiting inflammatory reaction induced by lipopolysaccharides in chondrocytes.Methods:Ten 8-week-old male SD rats were randomly divided into TGP group and blank group.The rats in TGP group were intragastric administrated with TGP in dosage of 0.32 g/kg,while the others in blank group were intragastric administrated with the same dose of normal saline,once per day for 7 consecutive days.At 1 hour after the last intragastric administration,their blood were fetched out from abdominal aorta and were made into TGP medicated serum and blank serum respectively and the serum were reserved at low temperature for future use.The chondrocytes of ten 4-week-old SD rats were isolated from knee articular cartilage and were cultured.The chondrocytes morphology were observed under optical microscope,and immunohistochemical identification were carried out by using typeⅡcollagenase.The second-generation chondrocytes were randomly divided into blank serum group,model group and TGP medicated serum group.The chondrocytes in blank serum group were cultured in dulbecco modified eagle medium(DMEM)supplemented with 10% blank serum.The chondrocytes in model group were cultured in DMEM supplemented with lipopolysaccharide(LPS)with concentration of 10 ng/ml and 10% blank serum.The chondrocytes in TGP medicated serum group were cultured in DMEM supplemented with LPS with concentration of 10 ng/ml and 10% TGP medicated serum.The chondrocytes in the 3 groups were intervened and cultured for continuous 8 hours.The content of matrix metalloproteinase(MMP)3 and MMP9 in chondrocytes were detected by using enzyme-linked immunoadsordent assay(ELISA).The expression levels of gene related to Wnt/β-catenin signaling pathway in chondrocytes were detected by using fluorescence quantitative RT-PCR method.The protein expressions of β-catenin and Frizzled-2 in chondrocytes were detected by using Western blot method.The protein expressions of β-catenin,glycogen synthasc kinase-3β(GSK-3β)and proteoglycans 1(PGS1)in chondrocytes were detected by using immunofluorescence assay(IFA).Results:The second-generation chondrocytes had typical biological characteristics of chondrocytes and their endochylemas and cytomembranes presented with brown-yellow positive staining.After 8-hour intervention by LPS,there was statistical difference in the content of MMP3 and MMP9 in chondrocytes between blank serum group,model group and TGP medicated serum group(34.019+/-1.036,44.645+/-2.473,32.941+/-1.792 ng/ml,F=36.060,P=0.000; 1.348+/-0.038,1.562+/-0.112,1.331+/-0.015 ng/ml,F=11.319,P=0.000).The content of MMP3 and MMP9 in chondrocytes were higher in model group compared to blank serum group(LSD-t=-7.016,P=0.000; LSD-t=-3.768,P=0.003)and were lower in TGP medicated serum group compared to model group(LSD-t=7.652,P=0.000; LSD-t=4.066,P=0.002).There was no statistical difference in the content of MMP3 and MMP9 in chondrocytes between blank serum group and TGP medicated serum group(LSD-t=0.635,P=0.549; LSD-t=0.299,P=0.770).After 8-hour intervention by LPS,there was statistical difference in gene expression levels of β-catenin,GSK-3β,Frizzled-2,Wnt-4 and CKI-ε between blank serum group,model group and TGP medicated serum group(1.000+/-0.275,2.258+/-0.206,1.431+/-0.304,F=36.709,P=0.000; 1.000+/-0.133,0.417+/-0.104,0.842+/-0.094,F=29.259,P=0.000; 1.000+/-0.191,1.737+/-0.238,1.445+/-0.337,F=7.027,P=0.015; 1.000+/-0.341,3.801+/-0.579,1.876+/-0.388,F=71.903,P=0.000; 1.000+/-0.309,2.208+/-0.708,1.441+/-0.421,F=64.178,P=0.000).The gene expression levels of β-catenin,Frizzled-2,Wnt-4 and CKI-εin chondrocytes were higher and the gene expression levels of GSK-3βwere lower in model group compared to blank serum group(LSD-t=-8.431,P=0.000; LSD-t=-3.723,P=0.005; LSD-t=8.062,P=0.000; LSD-t=-11.235,P=0.000,LSD-t=7.397,P=0.000).The gene expression levels of β-catenin,Frizzled-2,Wnt-4 and CKI-ε in chondrocytes were lower and the gene expression levels of GSK-3β were higher in TGP medicated serum group compared to model group(LSD-t=5.541,P=0.000; LSD-t=1.477,P=0.017; LSD-t=8.062,P=0.000; LSD-t=6.882,P=0.000; LSD-t=-5.387,P=0.000).The gene expression levels of β-catenin,Wnt-4 and CKI-ε in chondrocytes were lower in blank serum group compared to TGP medicated serum group(LSD-t=-2.289,P=0.018; LSD-t=-3.658,P=0.005; LSD-t=-4.352,P=0.002).There was no statistical difference in gene expression levels of GSK-3β and Frizzled-2 between blank serum group and TGP medicated serum group(LSD-t=2.009,P=0.075; LSD-t=-3.658,P=0.051).After 8-hour intervention by LPS,there was statistical difference in protein expressions of β-catenin and Frizzled-2 between blank serum group,model group and TGP medicated serum group(0.449+/-0.063,0.746+/-0.156,0.549+/-0.056,F=5.323,P=0.026; 1.348+/-0.038; 1.562+/-0.112; 1.331+/-0.015,F=6.291,P=0.034).The protein expressions of β-catenin and Frizzled-2 in chondrocytes were higher in model group compared to blank serum group(LSD-t=-11.235,P=0.005; LSD-t=-3.104,P=0.021).The protein expressions of β-catenin and Frizzled-2 in chondrocytes were lower in TGP medicated serum group compared to model group(LSD-t=6.883,P=0.037; LSD-t=3.039,P=0.023).The protein expressions of β-catenin in chondrocytes were lower in blank serum group compared to TGP medicated serum group(LSD-t=-4.352,P=0.002).There was no statistical difference in protein expressions of Frizzled-2 between blank serum group and TGP medicated serum group(LSD-t=-0.065,P=0.950).After 8-hour intervention by LPS,the chondrocytes presented with obvious green staining of β-catenin,GSK-3β and PGS1 protein.There was statistical difference in protein expressions of β-catenin,GSK-3β and PGS1 between blank serum group,model group and TGP medicated serum group(0.014+/-0.002,0.029+/-0.006,0.018+/-0.002,F=9.910,P=0.013; 0.380+/-0.011,0.237+/-0.015,0.287+/-0.002,F=56.639,P=0.000; 0.034+/-0.003,0.022+/-0.002,0.029+/-0.003,F=27.232,P=0.001).The protein expressions of β-catenin were higher and the protein expressions of GSK-3β and PGS1 were lower in model group compared to blank serum group(LSD-t=-4.103,P=0.006; LSD-t=1.048,P=0.000; t=7.365,P=0.000).The protein expressions of β-catenin were lower and the protein expressions of GSK-3β and PGS1 were higher in TGP medicated serum group compared to model group(LSD-t=-3.548,P=0.012; LSD-t=-3.657,P=0.011; LSD-t=-3.273,P=0.017).There was no statistical difference in protein expressions of β-catenin between blank serum group and TGP medicated serum group(LSD-t=-0.554,P=0.599).The protein expressions of GSK-3βand PGS1 were higher in blank serum group compared to TGP medicated serum group(LSD-t=6.827,P=0.000; LSD-t=4.092,P=0.010).Conclusion:TGP medicated serum can inhibit inflammatory reaction induced by LPS in chondrocytes and delay the articular cartilage degeneration.The mechanisms of action may be related to the regulation of Wnt/β-catenin signaling pathway,in which β-catenin gene,Frizzled-2 gene,GSK-3β gene,Wnt-4 gene and CKI-ε gene may be the important action targets.However,many factors can cause OA and there are many complicated ingredients in TGP medicated serum,further studies are needed to confirm the specific action targets.

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備注/Memo

備注/Memo:
基金項目:福建省科學技術廳重點資助項目(2014Y0064) 通訊作者:葉蕻芝 E-mail:[email protected]
更新日期/Last Update: 2017-12-29