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[1]全仁夫,胡華輝,黃小龍,等.電針秩邊穴和水道穴對(duì)急性脊髓不完全損傷大鼠腦皮質(zhì)代謝物的影響[J].中醫(yī)正骨,2016,28(11):1-6.
　QUAN Renfu,HU Huahui,HUANG Xiaolong,et al.Effect of electroacupuncture at Point BL54(Zhibian)and Point ST28(Shuidao)on cerebral cortex metabolites in rats with acute incomplete spinal cord injuries[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2016,28(11):1-6.
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電針秩邊穴和水道穴對(duì)急性脊髓不完全損傷大鼠腦皮質(zhì)代謝物的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第28卷
期數(shù):
2016年11期
頁碼:
1-6
欄目:
基礎(chǔ)研究
出版日期:
2016-11-20

文章信息/Info

Title:
Effect of electroacupuncture at Point BL54(Zhibian)and Point ST28(Shuidao)on cerebral cortex metabolites in rats with acute incomplete spinal cord injuries
作者:
全仁夫1; 胡華輝2; 黃小龍2; 劉飛2; 楊宗保3; 許晶晶3
1.浙江省杭州市蕭山區(qū)中醫(yī)院,浙江 杭州 311201;
2.浙江中醫(yī)藥大學(xué),浙江 杭州 310053;
3.廈門大學(xué),福建 廈門 361005
Author(s):
QUAN Renfu1; HU Huahui2; HUANG Xiaolong2; LIU Fei2; YANG Zongbao3; XU Jingjing3
1.Xiaoshan Hospital of Traditional Chinese Medicine,Hangzhou 311201,Zhejiang,China
2.Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China
3. Xiamen University,Xiamen 361005,Fujian,China
關(guān)鍵詞:
脊髓損傷;  電針;  代謝組學(xué);  穴; 秩邊;  穴; 水道;  核磁共振波譜法;  大鼠
Keywords:
spinal cord injuries;  electroacupuncture;  metabolomics;  point BL54(ZHIBIAN);  point ST28(SHUIDAO);  nuclear magnetic resonance spectroscopy;  rats
摘要:
目的:觀察電針秩邊穴和水道穴對(duì)急性脊髓不完全損傷大鼠腦皮質(zhì)代謝物的影響。方法:將36只雄性SD大鼠隨機(jī)分為假手術(shù)組、模型組、電針組,每組12只。采用改良Allens法將模型組和電針組大鼠制成T10急性脊髓不完全損傷模型,假手術(shù)組大鼠手術(shù)摘除T10椎板暴露硬脊膜后立即縫合。造模結(jié)束后,假手術(shù)組和模型組大鼠僅進(jìn)行抓取和固定; 電針組大鼠予以電針秩邊穴和水道穴,每天1次,連續(xù)治療1周。電針干預(yù)結(jié)束后斷頭處死所有大鼠,采集大鼠腦皮質(zhì)和T9～T10椎體對(duì)應(yīng)節(jié)段脊髓1 cm。將取得的脊髓組織制成切片,HE染色后光鏡下觀察脊髓組織形態(tài); 對(duì)取得的腦皮質(zhì)進(jìn)行磁共振波譜分析,比較3組大鼠腦皮質(zhì)代謝物的濃度。結(jié)果:至處死前各組均無大鼠死亡。假手術(shù)組大鼠脊髓結(jié)構(gòu)完整,神經(jīng)細(xì)胞形態(tài)正常、分布均勻,細(xì)胞膜、細(xì)胞核及組織間隙均正常; 模型組大鼠脊髓形態(tài)不完整,損傷區(qū)域有神經(jīng)組織殘缺,存在較多出血,組織疏松水腫,部分細(xì)胞核固縮,神經(jīng)細(xì)胞數(shù)量明顯減少,細(xì)胞周圍基質(zhì)消失呈空泡狀; 電針組大鼠脊髓形態(tài)結(jié)構(gòu)基本完整,組織中散在血細(xì)胞,組織水腫較輕,神經(jīng)細(xì)胞形態(tài)基本正常,空泡變性較輕。3組大鼠腦皮質(zhì)中異亮氨酸、谷氨酸、N-乙酰天門冬氨酸、甘油磷脂膽堿、肌醇、維生素C、乳酸、蘇氨酸、肌苷濃度比較,組間差異均有統(tǒng)計(jì)學(xué)意義(1.63±0.20,1.13±0.12,1.35±0.01,F=43.195,P=0.000; 9.51±0.40,8.66±0.74,9.94±1.25,F=6.728,P=0.004; 2.54±0.22,2.21±0.19,2.38±0.45,F=3.332,P=0.048; 9.47±1.26,8.10±1.34,9.38±0.88,F=5.070,P=0.012; 3.66±1.10,3.17±0.89,4.55±1.81,F=3.365,P=0.047; 2.45±0.33,2.15±0.21,2.34±0.17,F=4.388,P=0.020; 9.03±1.56,10.01±0.63,9.22±1.01,F=3.562,P=0.040; 4.12±0.28,3.21±0.13,3.69±0.44,F=4.972,P=0.013; 6.35±0.96,9.18±0.81,8.38±0.34,F=45.032,P=0.000)。假手術(shù)組和電針組異亮氨酸、谷氨酸、N-乙酰天門冬氨酸、甘油磷脂膽堿、肌醇、維生素C、蘇氨酸濃度均高于模型組(P=0.000,P=0.000; P=0.003,P=0.007; P=0.001,P=0.035; P=0.017,P=0.011; P=0.045,P=0.023; P=0.016,P=0.026; P=0.000,P=0.001),乳酸和肌苷的濃度均低于模型組(P=0.017,P=0.030; P=0.000,P=0.000); 假手術(shù)組異亮氨酸和蘇氨酸的濃度均高于電針組(P=0.009; P=0.010),肌苷濃度低于電針組(P=0.005); 假手術(shù)組和電針組谷氨酸、N-乙酰天門冬氨酸、甘油磷脂膽堿、肌醇、維生素C、乳酸的濃度比較,組間差異均無統(tǒng)計(jì)學(xué)意義(P=0.322; P=0.469; P=0.850; P=0.106; P=0.325; P=0.684)。結(jié)論:電針急性脊髓不完全損傷大鼠的秩邊穴和水道穴,有利于其脊髓損傷恢復(fù),并可提高其腦皮質(zhì)中異亮氨酸、谷氨酸、N-乙酰天門冬氨酸、甘油磷脂膽堿、肌醇、維生素C、蘇氨酸的濃度,降低乳酸和肌苷的濃度。
Abstract:
Objective:To observe the effect of electroacupuncture at Point BL54(Zhibian)and Point ST28(Shuidao)on cerebral cortex metabolites in rats with acute incomplete spinal cord injuries.Methods:Thirty-six male SD rats were randomly divided into sham operated group,model group and electroacupuncture group,12 cases in each group.The T10 acute incomplete spinal cord injuries models were built by modified Allen's method in rats of model group and electroacupuncture group,while T10 vertebral plates were removed to expose the endorachis and the incision were sewed in rats of sham-operated group.After the end of the modeling,the rats in sham-operated group and model group were merely catched and fixed,while the rats in electroacupuncture group were treated with electroacupuncture at Point BL54(Zhibian)and Point ST28(Shuidao),once a day for consecutive 1 week.After the end of electroacupuncture intervention,all rats were executed with decollation and the cerebral cortexs and 1 cm spinal cord at T9-10 vertebral segment were collected.The spinal cord tissues were sectioned for HE staining and the spinal cord tissue morphology were observed under light microscope.The cerebral cortexs were analyzed by using nuclear magnetic resonance spectroscopy,and the concentrations of cerebral cortex metabolites were compared between the 3 groups.Results:No rats died in each group before execution.The structure of spinal cord was complete and the nerve cells were uniformly distributed in normal shape and their cellular membrane,cellular nucleus and tissue spaces were normal in rats of sham-operated group.The shape of spinal cord was incomplete in rats of model group and the injured region of spinal cord presented with(1)incomplete nervous tissues,(2)massive bleeding,(3)tissue edema,(4)karyopycnosis in some nerve cells,(5)significant decrease of nerve cells and(6)vacuolization in cell matrixs.The shape and structure of spinal cord were basically complete in rats of electroacupuncture group,and the injured region of spinal cord presented with(1)sporadic blood cells,(2)slight tissue edema,(3)basically normal shape of nerve cells and(4)slight vacuolization in cell matrixs.There was statistical difference in the concentrations of isoleucine,glutamic acid,N-acetyl-aspartate,glycerophosphorylcholine,inositol,vitamin C,lactic acid,threonine and inosine in the cerebral cortex beween the 3 groups(1.63+/-0.20,1.13+/-0.12,1.35+/-0.01,F=43.195,P=0.000; 9.51+/-0.40,8.66+/-0.74,9.94+/-1.25,F=6.728,P=0.004; 2.54+/-0.22,2.21+/-0.19,2.38+/-0.45,F=3.332,P=0.048; 9.47+/-1.26,8.10+/-1.34,9.38+/-0.88,F=5.070,P=0.012; 3.66+/-1.10,3.17+/-0.89,4.55+/-1.81,F=3.365,P=0.047; 2.45+/-0.33,2.15+/-0.21,2.34+/-0.17,F=4.388,P=0.020; 9.03+/-1.56,10.01+/-0.63,9.22+/-1.01,F=3.562,P=0.040; 4.12+/-0.28,3.21+/-0.13,3.69+/-0.44,F=4.972,P=0.013; 6.35+/-0.96,9.18+/-0.81,8.38±0.34,F=45.032,P=0.000).The concentrations of isoleucine,glutamic acid,N-acetyl-aspartate,glycerophosphorylcholine,inositol,vitamin C and threonine in the cerebral cortex were higher in sham-operated group and electroacupuncture group compared to model group(P=0.000,P=0.000; P=0.003,P=0.007; P=0.001,P=0.035; P=0.017,P=0.011; P=0.045,P=0.023; P=0.016,P=0.026; P=0.000,P=0.001),and the concentrations of lactic acid and inosine were lower in sham-operated group and electroacupuncture group compared to model group(P=0.017,P=0.030; P=0.000,P=0.000).The concentrations of isoleucine and threonine were higher in sham-operated group compared to electroacupuncture group(P=0.009; P=0.010),and the concentrations of inosine were lower in sham-operated group compared to electroacupuncture group(P=0.005).There was no statistical difference in the concentrations of glutamic acid,N-acetyl-aspartate,glycerophosphorylcholine,inositol,vitamin C and lactic acid in the cerebral cortex between sham-operated group and electroacupuncture group(P=0.322; P=0.469; P=0.850; P=0.106; P=0.325; P=0.684).Conclusion:Electroacupuncture at Point BL54(Zhibian)and Point ST28(Shuidao)is helpful to the recovery of impaired spinal cord of rats with acute incomplete spinal cord injury,and it can increase the concentration of isoleucine,glutamic acid,N-acetyl-aspartate,glycerophosphorylcholine,inositol,vitamin C and threonine in the cerebral cortex of rats.

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備注/Memo

備注/Memo:
基金項(xiàng)目:浙江省自然科學(xué)基金項(xiàng)目(LY15H270003); 浙江省中醫(yī)藥科技計(jì)劃項(xiàng)目(2015ZZ017)
通訊作者:全仁夫 E-mail:[email protected]
更新日期/Last Update: 2016-11-30