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[1]汪倩倩,劉春芳,姜宜妮,等.補(bǔ)腎方對(duì)激素性股骨頭壞死大鼠股骨頭血管形態(tài)和血液狀態(tài)的影響[J].中醫(yī)正骨,2016,28(06):1-11.
　WANG Qianqian,LIU Chunfang,JIANG Yini,et al.Effect of Bushen Fang(補(bǔ)腎方)on blood vessel morphous of femoral head and blood state in rats with steroid-induced necrosis of femoral head[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2016,28(06):1-11.
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補(bǔ)腎方對(duì)激素性股骨頭壞死大鼠股骨頭血管形態(tài)和血液狀態(tài)的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第28卷
期數(shù):
2016年06期
頁(yè)碼:
1-11
欄目:
基礎(chǔ)研究
出版日期:
2016-06-20

文章信息/Info

Title:
Effect of Bushen Fang(補(bǔ)腎方)on blood vessel morphous of femoral head and blood state in rats with steroid-induced necrosis of femoral head
作者:
汪倩倩1; 劉春芳2; 姜宜妮2; 王慧2; 陳衛(wèi)衡3; 林娜2
1.廣州中醫(yī)藥大學(xué),廣州 510006;
2.中國(guó)中醫(yī)科學(xué)院中藥研究所,北京 100700;
3.中國(guó)中醫(yī)科學(xué)院望京醫(yī)院,北京 100102
Author(s):
WANG Qianqian1; LIU Chunfang2; JIANG Yini2; WANG Hui2; CHEN Weiheng3; LIN Na2
1.Guangzhou University of Chinese Medicine,Guangzhou 510006,China
2.Institute of Chinese materia medica of China Academy of Chinese Medical sciences,Beijing 100700,China
3.Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102,China
關(guān)鍵詞:
股骨頭壞死;  大鼠; Wistar;  補(bǔ)腎方;  血管形態(tài);  血液狀態(tài);  動(dòng)物實(shí)驗(yàn)
Keywords:
femur head necrosis;  rats; wistar;  Bushen Fang;  morphous of blood vessel;  state of blood;  animal experimentation
摘要:
目的:探討補(bǔ)腎方對(duì)激素性股骨頭壞死(osteonecroois of the femoral head,ONFH)大鼠股骨頭血管形態(tài)和血液狀態(tài)的影響。方法:將120只雄性 Wistar大鼠隨機(jī)分為空白組、模型組、健骨生丸組、補(bǔ)腎方高劑量組、補(bǔ)腎方中劑量組和補(bǔ)腎方低劑量組,每組20只。除空白組外,其余5組大鼠臀肌注射甲潑尼龍琥珀酸鈉,連續(xù)注射3 d,建立激素性O(shè)NFH模型。甲潑尼龍琥珀酸鈉注射完后,空白組、模型組自由飲食; 健骨生丸組按1.68 g·kg-1以健骨生丸灌胃,每天1次,連續(xù)6周; 補(bǔ)腎方高、中、低劑量組分別按21.2 g·kg-1、10.6 g·kg-1、5.3 g·kg-1以補(bǔ)腎方灌胃,每天1次,連續(xù)6周。藥物干預(yù)結(jié)束后,先從各組隨機(jī)選取10只大鼠,麻醉后腹主動(dòng)脈取血,動(dòng)物死亡后,取雙側(cè)股骨頭制成石蠟切片。將所取腹主動(dòng)脈血分成2份,一份取血清測(cè)定甘油三酯(triglyceride,TG)、總膽固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein,HDL)、低密度脂蛋白(low density lipoprotein,LDL)、載脂蛋白A1(apolipoprotein A1,ApoA1)及ApoB; 另一份取全血以旋轉(zhuǎn)法測(cè)定全血黏度低切值、中切值和高切值,取血漿檢測(cè)血漿黏度。制成的股骨頭組織切片分成2份,一份進(jìn)行HE染色,光學(xué)顯微鏡下觀察骨組織形態(tài); 另一份進(jìn)行免疫組織化學(xué)染色,測(cè)定血管內(nèi)皮生長(zhǎng)因子(vascular endothelial growth factor,VEGF)和FLK1蛋白表達(dá)情況。各組剩余的10只大鼠應(yīng)用血管造影結(jié)合Micro-CT掃描技術(shù)測(cè)定股骨頭中血管體積、血管表面積、血管體積分?jǐn)?shù)及血管厚度。結(jié)果:①血脂檢測(cè)結(jié)果。6組大鼠血清TG、TC、LDL、HDL、ApoA1和ApoB含量比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=4.538,P=0.004; F=3.322,P=0.018; F=2.681,P=0.043; F=2.621,P=0.047; F=2.400,P=0.035; F=3.741,P=0.010)。空白組、健骨生丸組、補(bǔ)腎方中劑量組和補(bǔ)腎方高劑量組TG、TC、LDL、ApoB含量均低于模型組(P=0.000,P=0.021,P=0.009,P=0.032; P=0.008,P=0.016,P=0.031,P=0.030; P=0.009,P=0.017,P=0.036,P=0.031; P=0.005,P=0.013,P=0.031,P=0.025),HDL、ApoA1含量均高于模型組(P=0.019,P=0.034; P=0.041,P=0.034; P=0.040,P=0.031; P=0.035,P=0.029); 補(bǔ)腎方低劑量組TC含量低于模型組(P=0.023),TG、LDL、HDL、ApoA1、ApoB含量與模型組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.297,P=0.315,P=0.189,P=0.084,P=0.333); 補(bǔ)腎方低劑量組血清TG、TC、LDL、ApoB含量均高于健骨生丸組(P=0.037,P=0.018,P=0.041,P=0.047),HDL、ApoA1含量均低于健骨生丸組(P=0.046,P=0.043); 補(bǔ)腎方中劑量組和健骨生丸組血清TG、TC、LDL、HDL、ApoA1、ApoB含量比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.080,P=0.440,P=0.375,P=0.204,P=0.130,P=0.389); 補(bǔ)腎方高劑量組血清TG、TC、LDL、ApoB含量均低于健骨生丸組(P=0.019,P=0.022,P=0.024,P=0.039),HDL含量高于健骨生丸組(P=0.043),2組ApoA1含量比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.094); 補(bǔ)腎方中劑量組和高劑量組血清TG、TC、LDL、ApoB含量均低于低劑量組(P=0.033,P=0.021,P=0.042,P=0.042; P=0.021,P=0.019,P=0.018,P=0.034),HDL含量均高于低劑量組(P=0.048; P=0.042),2組ApoA1含量與補(bǔ)腎方低劑量組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.053; P=0.057); 補(bǔ)腎方高劑量組血清TG、TC、LDL、ApoB含量均低于中劑量組(P=0.029,P=0.020,P=0.020,P=0.035),HDL含量高于中劑量組(P=0.045),2組ApoA1含量比較,組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.239)。②血液流變學(xué)指標(biāo)檢測(cè)結(jié)果。6組大鼠全血黏度低切值、全血黏度中切值及血漿黏度比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=3.291,P=0.019; F=3.256,P=0.020; F=3.779,P=0.010); 6組全血黏度高切值比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(F=2.460,P=0.059)。空白組、健骨生丸組、補(bǔ)腎方中劑量組和補(bǔ)腎方高劑量組全血黏度低切值、全血黏度中切值及血漿黏度均低于模型組(P=0.017,P=0.033,P=0.011; P=0.026,P=0.043,P=0.040; P=0.028,P=0.012,P=0.028; P=0.023,P=0.010,P=0.022); 補(bǔ)腎方低劑量組全血黏度低切值、全血黏度中切值及血漿黏度與模型組比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.085,P=0.069,P=0.094); 補(bǔ)腎方低劑量組血漿黏度高于健骨生丸組(P=0.049),2組全血黏度低切值、全血黏度中切值比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.054,P=0.057); 補(bǔ)腎方中劑量組和健骨生丸組全血黏度低切值、全血黏度中切值及血漿黏度比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.091,P=0.083,P=0.055); 補(bǔ)腎方高劑量組全血黏度中切值和血漿黏度 均低于健骨生丸組(P=0.045,P=0.014),2組全血黏度低切值比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.214); 補(bǔ)腎方中劑量組全血黏度低切值、血漿黏度均低于低劑量組(P=0.048,P=0.032),2組全血黏度中切值比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.051); 補(bǔ)腎方高劑量組全血黏度低切值、全血黏度中切值及血漿黏度均低于低劑量組(P=0.030,P=0.048,P=0.013); 補(bǔ)腎方高劑量組全血黏度低切值、血漿黏度均低于中劑量組(P=0.049,P=0.027),2組全血黏度中切值比較,組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.052)。③VEGF蛋白和FLK1蛋白測(cè)定結(jié)果。6組大鼠股骨頭內(nèi)VEGF蛋白和FLK1蛋白表達(dá)量比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=9.519,P=0.000; F=5.317,P=0.009)。空白組、健骨生丸組、補(bǔ)腎方中劑量組和補(bǔ)腎方高劑量組VEGF蛋白和FLK1蛋白表達(dá)量均高于模型組(P=0.000,P=0.000; P=0.005,P=0.009; P=0.004,P=0.008; P=0.000,P=0.000); 補(bǔ)腎方低劑量組VEGF蛋白表達(dá)量與模型組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.051),FLK1蛋白表達(dá)量高于模型組(P=0.047); 補(bǔ)腎方低劑量組VEGF蛋白和FLK1蛋白表達(dá)量均低于健骨生丸組(P=0.041,P=0.036); 補(bǔ)腎方中劑量組VEGF蛋白和FLK1蛋白表達(dá)量與健骨生丸組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.175; P=0.221); 補(bǔ)腎方高劑量組VEGF蛋白和FLK1蛋白表達(dá)量均高于健骨生丸組(P=0.045; P=0.047); 補(bǔ)腎方中劑量組和高劑量組VEGF蛋白、FLK1蛋白表達(dá)量均高于低劑量組(P=0.047,P=0.044; P=0.016,P=0.011); 補(bǔ)腎方高劑量組FLK1蛋白表達(dá)量高于中劑量組(P=0.042),2組VEGF蛋白表達(dá)量比較,組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.051)。④股骨頭內(nèi)血管Micro-CT檢查結(jié)果。6組大鼠股骨頭血管體積、血管表面積、血管體積分?jǐn)?shù)、血管厚度比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=36.442,P=0.000; F=7.080,P=0.000; F=27.869,P=0.000; F=8.371,P=0.000)。空白組、健骨生丸組、補(bǔ)腎方中劑量組、補(bǔ)腎方高劑量組血管體積、血管表面積、血管體積分?jǐn)?shù)、血管厚度均大于模型組(P=0.000,P=0.000,P=0.000,P=0.000; P=0.009,P=0.003,P=0.002,P=0.001; P=0.000,P=0.001,P=0.001,P=0.000; P=0.007,P=0.015,P=0.011,P=0.005); 補(bǔ)腎方低劑量組與模型組血管體積、血管表面積、血管體積分?jǐn)?shù)、血管厚度比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.051,P=0.052,P=0.082,P =0.064); 補(bǔ)腎方低劑量組、補(bǔ)腎方中劑量組與健骨生丸組血管體積、血管表面積、血管體積分?jǐn)?shù)、血管厚度比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.057,P=0.063,P=0.051,P=0.052; P=1.000,P=0.222,P=1.000,P=0.813); 補(bǔ)腎方高劑量組血管體積、血管表面積、血管體積分?jǐn)?shù)、血管厚度均大于健骨生丸組(P=0.000,P=0.017,P=0.000,P=0.010); 補(bǔ)腎方中劑量組和高劑量組血管體積、血管表面積、血管體積分?jǐn)?shù)、血管厚度均大于低劑量組(P=0.000,P=0.023,P=0.001,P=0.021; P=0.000,P=0.015,P=0.000,P=0.007); 補(bǔ)腎方高劑量組血管體積、血管表面積、血管體積分?jǐn)?shù)、血管厚度均大于中劑量組(P=0.000,P=0.019,P=0.000,P=0.009)。結(jié)論:補(bǔ)腎方能促進(jìn)激素性O(shè)NFH大鼠股骨血管修復(fù),改善股骨頭血液微循環(huán)狀態(tài),其作用可能與補(bǔ)腎方增加股骨頭內(nèi)VEGF和FLK1蛋白表達(dá)有關(guān),且中劑量補(bǔ)腎方的療效與健骨生丸相當(dāng),高劑量補(bǔ)腎方的療效優(yōu)于健骨生丸。
Abstract:
Objective:To explore the effect of Bushen Fang(補(bǔ)腎方,BSF)on blood vessel morphous of femoral head and blood state in rats with steroid-induced necrosis of femoral head(SNFH).Methods:One hundred and twenty Wistar male rats were randomly divided into blank group,model group,Jiangusheng Wan(健骨生丸,JGSW)group,BSF high-dose group,BSF middle-dose group and BSF low-dose group,20 cases in each group.The rats in model group,JGSW group,BSF high-dose group,BSF middle-dose group and BSF low-dose group were treated with intra-gluteal injection of methylprednisolone sodium succinate for consecutive 3 days to build the SNFH models.Then the rats in blank group and model group were permitted to drink and eat freely.The rats in JGSW group were intragastric administrated with JGSW(1.68 g/kg),once a day for consecutive 6 weeks; while the others in BSF high-dose group,BSF middle-dose group and BSF low-dose group were intragastric administrated with BSF(21.2,10.6 and 5.3 g/kg,respectively),once a day for consecutive 6 weeks.After the end of drug intervention,10 rats were randomly selected from each group,and their blood were fetched out from aorta abdominalis after anesthesia and the bilateral femoral heads were fetched out for making paraffin sections after the rats died.The blood were divided into 2 parts,and one of them were applied to measure the serum contents of triglyceride(TG),total cholesterol(TC),high density lipoprotein(HDL),low density lipoprotein(LDL),apolipoprotein A1(ApoA1)and ApoB; while the other portion were applied to measure the high,medium and low shear whole blood viscosity and the plasma viscosity.The acquired tissue sections of femoral head were divided into 2 parts,and one of them were received HE staining for observing the bone tissue morphology under optical microscope,while the other portion were received immunohistochemical staining for detecting the expression of vascular endothelial growth factor(VEGF)protein and FLK1 protein.The remaining 10 rats in each group were used to measure the volume, surface area,volume fraction and thickness of blood vessel in femoral head by using angiography combined with Micro-CT scanning technique.Results:There was statistical difference in serum content of TG,TC,LDL,HDL,ApoA1 and ApoB between the 6 groups(F=4.538,P=0.004; F=3.322,P=0.018; F=2.681,P=0.043; F=2.621,P=0.047; F=2.400,P=0.035; F=3.741,P=0.010).The serum content of TG,TC,LDL and ApoB were lower and the serum content of HDL and ApoA1 were higher in blank group,JGSW group,BSF middle-dose group and BSF high-dose group compared to model group(P=0.000,P=0.021,P=0.009,P=0.032; P=0.008,P=0.016,P=0.031,P=0.030; P=0.009,P=0.017,P=0.036,P=0.031; P=0.005,P=0.013,P=0.031,P=0.025; P=0.019,P=0.034; P=0.041,P=0.034; P=0.040,P=0.031; P=0.035,P=0.029).The serum content of TC were lower in BSF low-dose group compared to model group(P=0.023),and there was no statistical difference in serum content of TG,LDL,HDL,ApoA1 and ApoB between BSF low-dose group and model group(P=0.297,P=0.315,P=0.189,P=0.084,P=0.333).The serum content of TG,TC,LDL and ApoB were higher and the serum content of HDL and ApoA1 were lower in BSF low-dose group compared to JGSW group(P=0.037,P=0.018,P=0.041,P=0.047,P=0.046,P=0.043).There was no statistical difference in serum content of TG,TC,LDL,HDL,ApoA1 and ApoB between BSF middle-dose group and JGSW group(P=0.080,P=0.440,P=0.375,P=0.204,P=0.130,P=0.389).The serum content of TG,TC,LDL and ApoB were lower and the serum content of HDL was higher in BSF high-dose group compared to JGSW group(P=0.019,P=0.022,P=0.024,P=0.039,P=0.043),and there was no statistical difference in serum content of ApoA1 between the two groups(P=0.094).The serum content of TG,TC,LDL and ApoB were lower and the serum content of HDL was higher in BSF middle-dose group and high-dose group compared to low-dose group(P=0.033,P=0.021,P=0.042,P=0.042; P=0.021,P=0.019,P=0.018,P=0.034; P=0.048; P=0.042),and there was no statistical difference in serum content of ApoA1 between BSF middle-dose group and BSF low-dose group and between BSF high-dose group and BSF low-dose group(P=0.053; P=0.057).The serum content of TG,TC,LDL and ApoB were lower and the serum content of HDL was higher in BSF high-dose group compared to BSF middle-dose group(P=0.029,P=0.020,P=0.020,P=0.035,P=0.045),and there was no statistical difference in serum content of ApoA1 between the two groups(P=0.239).The detection results of hemorheological indexs showed that there was statistical difference in low and medium shear whole blood viscosity and plasma viscosity between the 6 groups(F=3.291,P=0.019; F=3.256,P=0.020; F=3.779,P=0.010),and there was no statistical difference in the high shear whole blood viscosity between the 6 groups(F=2.460,P=0.059).The low and medium shear whole blood viscosity and plasma viscosity were lower in blank group,JGSW group,BSF middle-dose group and BSF high-dose group compared to model group(P=0.017,P=0.033,P=0.011; P=0.026,P=0.043,P=0.040; P=0.028,P=0.012,P=0.028; P=0.023,P=0.010,P=0.022).There was no statistical difference in low and medium shear whole blood viscosity and plasma viscosity between BSF low-dose group and model group(P=0.085,P=0.069,P=0.094).The plasma viscosity was higher in BSF low-dose group compared to JGSW group(P=0.049),and there was no statistical difference in low and medium shear whole blood viscosity between the two groups(P=0.054,P=0.057).There was no statistical difference in low and medium shear whole blood viscosity and plasma viscosity between BSF middle-dose group and JGSW group(P=0.091,P=0.083,P=0.055).The medium shear whole blood viscosity and plasma viscosity were lower in BSF high-dose group compared to JGSW group(P=0.045,P=0.014),and there was no statistical difference in the low shear whole blood viscosity between the two groups(P=0.214).The low shear whole blood viscosity and plasma viscosity were lower in BSF middle-dose group compared to BSF low-dose group(P=0.048,P=0.032),and there was no statistical difference in the medium shear whole blood viscosity between the two groups(P=0.051).The low and medium shear whole blood viscosity and plasma viscosity were lower in BSF high-dose group compared to BSF low-dose group(P=0.030,P=0.048,P=0.013).The low shear whole blood viscosity and plasma viscosity were lower in BSF high-dose group compared to BSF middle-dose group(P=0.049,P=0.027),and there was no statistical difference in the medium shear whole blood viscosity between the two groups(P=0.052).There was statistical difference in the expression of VEGF protein and FLK1 protein in femoral heads between the 6 groups(F=9.519,P=0.000; F=5.317,P=0.009).The VEGF and FLK1 protein expression were higher in blank group,JGSW group,BSF middle-dose group and BSF high-dose group compared to model group(P=0.000,P=0.000; P=0.005,P=0.009; P=0.004,P=0.008; P=0.000,P=0.000).There was no statistical difference in the VEGF protein expression between BSF low-dose group and model group(P=0.051),while the FLK1 protein expression were higher in BSF low-dose group comared to model group(P=0.047).The VEGF and FLK1 protein expression were lower in BSF low-dose group compared to JGSW group(P=0.041,P=0.036).There was no statistical difference in the VEGF and FLK1 protein expression between BSF middle-dose group and JGSW group(P=0.175; P=0.221).The VEGF and FLK1 protein expression were higher in BSF high-dose group compared to JGSW group(P=0.045; P=0.047).The VEGF and FLK1 protein expression were higher in BSF middle-dose group and high-dose group compared to low-dose group(P=0.047,P=0.044; P=0.016,P=0.011).The FLK1 protein expression was higher in BSF high-dose group compared to middle-dose group(P=0.042),and there was no statistical difference in the VEGF protein expression between the two groups(P=0.051).The results of Micro-CT examination showed that there was statistical difference in the volumes,surface area,volume fraction and thickness of blood vessels in femoral head between the 6 groups(F=36.442,P=0.000; F=7.080,P=0.000; F=27.869,P=0.000; F=8.371,P=0.000).The volumes,surface area,volume fraction and thickness of blood vessels were larger in blank group,JGSW group,BSF middle-dose group and BSF high-dose group compared to model group(P=0.000,P=0.000,P=0.000,P=0.000; P=0.009,P=0.003,P=0.002,P=0.001; P=0.000,P=0.001,P=0.001,P=0.000; P=0.007,P=0.015,P=0.011,P=0.005).There was no statistical difference in the volumes,surface area,volume fraction and thickness of blood vessels between BSF low-dose group and model group(P=0.051,P=0.052,P=0.082,P=0.064).There was no statistical difference in the volumes,surface area,volume fraction and thickness of blood vessels between BSF low-dose group and JGSW group and between BSF middle-dose group and JGSW group(P=0.057,P=0.063,P=0.051,P=0.052; P=1.000,P=0.222,P=1.000,P=0.813).The volumes,surface area,volume fraction and thickness of blood vessels were larger in BSF high-dose group compared to JGSW group(P=0.000,P=0.017,P=0.000,P=0.010).The volumes,surface area,volume fraction and thickness of blood vessels were larger in BSF middle-dose group and high-dose group compared to low-dose group(P=0.000,P=0.023,P=0.001,P=0.021; P=0.000,P=0.015,P=0.000,P=0.007).The volumes,surface area,volume fraction and thickness of blood vessels were larger in BSF high-dose group compared to middle-dose group(P=0.000,P=0.019,P=0.000,P=0.009).Conclusion:Application of BSF can promote femoral blood vessel repair and improve blood microcirculation of femoral head in rats with SNFH,and the effect may be related to the increase of expression of VEGF protein and FLK1 protein in the femoral head.The middle-dose BSF is similar to JGSW while the high-dose BSF surpasses JGSW in the curative effect.

參考文獻(xiàn)/References:

[1] 王榮田,陳衛(wèi)衡,林娜,等.股骨頭壞死的病因構(gòu)成及發(fā)病特征分析[J].中國(guó)骨與關(guān)節(jié)損傷雜志,2009,24(9):792-795.
[2] 郝廷,劉萬(wàn)林,蘇秀蘭.血管內(nèi)皮生長(zhǎng)因子與激素性股骨頭壞死關(guān)系的研究進(jìn)展[J].臨床醫(yī)學(xué)工程,2009,16(1):85-87.
[3] 陳衛(wèi)衡,劉道兵,張強(qiáng),等.SARS后股骨頭壞死的證候特點(diǎn)及治療方案優(yōu)化研究(下)[J].中國(guó)中醫(yī)藥現(xiàn)代遠(yuǎn)程教育,2006,4(11):54-57.
[4] 暴淑英,趙慶國(guó),畢黎琦.激素性股骨頭壞死早期細(xì)胞凋亡相關(guān)基因表達(dá)及阿侖磷酸鈉的干預(yù)[J].中國(guó)組織工程研究與臨床康復(fù),2008,12(46):9095-9099.
[5] Jiang Y,Zhang Y,Zhang H,et al.Pravastatin prevents steroid-induced osteonecrosis in rats by suppressing PPARγ expression and activating Wnt signaling pathway[J].Exp Biol Med(Maywood),2014,239(3):347-355.
[6] Jiang Y,Liu C,Chen W,et al.Tetramethylpyrazine enhances vascularization and prevents osteonecrosis in steroid-treated rats[J].Biomed Res Int,2015:315850.
[7] Jiang Y,Zhang Y,Chen W,et al.Achyranthes bidentata extract exerts osteoprotective effects on steroid-induced osteonecrosis of the femoral head in rats by regulating RANKL/RANK/OPG signaling[J].J Transl Med,2014,12:334.
[8] Jiang Y,Liu D,Kong X,et al.Huogu I formula prevents steroid-induced osteonecrosis in rats by down-regulating PPARgamma expression and activating wnt/LRP5/beta-catenin signaling[J].J Tradit Chin Med,2014,34(3):342-350.
[9] 焦慶豐.激素性股骨頭壞死發(fā)病機(jī)制的研究進(jìn)展[J].臨床骨科雜志,2010,13(3):329-331.
[10] Weinstein RS.Glucocorticoid-induced osteonecrosis[J].Endocrine,2012,41(2):183-190.
[11] 邵陽(yáng),趙曉艷,馬勇.激素性股骨頭壞死發(fā)病機(jī)制的研究進(jìn)展[J].中國(guó)中醫(yī)骨傷科雜志,2012,20(8):88-90.
[12] Li J,Fan L,Yu Z,et al.The effect of deferoxamine on angiogenesis and bone repair in steroid-induced osteonecrosis of rabbit femoral heads[J].Exp Biol Med(Maywood),2015,240(2):273-280.
[13] Saito S,Ohzono K,Ono K.Early arteriopathy and postulated pathogenesis of osteonecrosis of the femoral head. The intracapital arterioles[J].Clin Orthop Relat Res,1992,(277):98-110.
[14] 徐西林,趙永蘭,張曉峰,等.活骨注射液髖關(guān)節(jié)腔灌注對(duì)兔股骨頭壞死模型血管內(nèi)皮生長(zhǎng)因子表達(dá)的動(dòng)態(tài)影響[J].中醫(yī)正骨,2015,27(8):1-6.
[15] 鄧沂,張曉剛,任遠(yuǎn),等.中醫(yī)對(duì)股骨頭壞死的認(rèn)識(shí)[J].甘肅中醫(yī)學(xué)院學(xué)報(bào),1998,15(4):56-58.
[16] 萬(wàn)蓉,李莉,孔祥英,等.不同治法方藥對(duì)激素性股骨頭壞死雞股骨頭OPG,RANKL mRNA表達(dá)的影響[J].中國(guó)實(shí)驗(yàn)方劑學(xué)雜志,2011,17(8):149-153.
[17] 孔祥英,萬(wàn)蓉,李莉,等.不同治法方藥對(duì)激素性股骨頭壞死雞成骨相關(guān)因子的影響[J].中國(guó)中藥雜志,2011,36(5):614-617.
[18] 王慧,劉春芳,姜宜妮,等.補(bǔ)腎方對(duì)激素性股骨頭壞死大鼠的骨修復(fù)作用[J].中國(guó)實(shí)驗(yàn)方劑學(xué)雜志,2016,22(1):88-92.
[19] Sun Y,Feng Y,Zhang C,et al.Beneficial effect of autologous transplantation of endothelial progenitor cells on steroid-induced femoral head osteonecrosis in rabbits[J].Cell Transplant,2011,20(2):233-243.
[20] Sun Y,Feng Y,Zhang C.The effect of bone marrow mononuclear cells on vascularization and bone regeneration in steroid-induced osteonecrosis of the femoral head[J].Joint Bone Spine,2009,76(6):685-690.
[21] Weinstein RS,Wan C,Liu Q,et al.Endogenous glucocorticoids decrease skeletal angiogenesis,vascularity,hydration,and strength in aged mice[J].Aging Cell,2010,9(2):147-161.
[22] Drescher W,Schlieper G,Floege J,et al.Steroid-related osteonecrosis—an update[J].Nephrol Dial Transplant,2011,26(9):2728-2731.
[23] 趙宏斌,李林芝,李世和.激素相關(guān)性股骨頭壞死股骨頭內(nèi)VEGF和BMP-2的表達(dá)[J].中國(guó)矯形外科雜志,2006,14(11):842-844.
[24] 胡志明,王海彬,李祖國(guó),等.TNFα和VEGF在激素性股骨頭壞死中的變化[J].中國(guó)矯形外科雜志,2006,14(12):912-914.
[25] 楊操,楊述華,杜靖遠(yuǎn),等.血管內(nèi)皮生長(zhǎng)因子基因轉(zhuǎn)染促進(jìn)股骨頭壞死修復(fù)[J].臨床骨科雜志,2004,7(1):90-93.

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備注/Memo

備注/Memo:
基金項(xiàng)目:國(guó)家自然科學(xué)基金項(xiàng)目( 81173417,81373656 )
通訊作者:林娜 E-mail:[email protected]
更新日期/Last Update: 2016-06-30