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[1]鄭春松,葉蕻芝,李西海,等.獨活寄生湯含藥血清對白細胞介素1β誘導(dǎo)的 退變關(guān)節(jié)軟骨細胞中基質(zhì)金屬蛋白酶 和環(huán)氧化酶2表達的影響[J].中醫(yī)正骨,2015,27(12):1-6.
　ZHENG Chunsong,YE Hongzhi,LI Xihai,et al.Impact of Duhuo Jisheng Tang(獨活寄生湯)medicated serum on expression of matrix metalloproteinase and cyclooxygenase 2 in degenerative articular chondrocytes induced by interleukin-1 beta[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(12):1-6.
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獨活寄生湯含藥血清對白細胞介素1β誘導(dǎo)的 退變關(guān)節(jié)軟骨細胞中基質(zhì)金屬蛋白酶 和環(huán)氧化酶2表達的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期數(shù):
2015年12期
頁碼:
1-6
欄目:
基礎(chǔ)研究
出版日期:
2015-12-30

文章信息/Info

Title:
Impact of Duhuo Jisheng Tang(獨活寄生湯)medicated serum on expression of matrix metalloproteinase and cyclooxygenase 2 in degenerative articular chondrocytes induced by interleukin-1 beta
作者:
鄭春松1; 葉蕻芝1; 李西海2; 付長龍1; 吳廣文2; 陳星強2; 劉獻祥1
1.福建中醫(yī)藥大學(xué)中西醫(yī)結(jié)合研究院,福建 福州 350122;
2.福建省中西醫(yī)結(jié)合老年性疾病重點實驗室,福建 福州 350122
Author(s):
ZHENG Chunsong1; YE Hongzhi1; LI Xihai2; FU Changlong1; WU Guangwen2; CHEN Xingqiang2; LIU Xianxiang1
1.Academy of Integrated Medicine affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China
2.Fujian Key Laboratory of Integrated Medicine on Geriatrics,Fuzhou 350122,Fujian,China
關(guān)鍵詞:
骨關(guān)節(jié)炎;  獨活寄生湯;  軟骨細胞;  基質(zhì)金屬蛋白酶類;  環(huán)氧化酶2;  大鼠; Sprague-Dawley;  動物實驗
Keywords:
osteoarthritis;  Duhuo Jisheng Tang;  chondrocytes;  matrix metalloproteinases;  cyclooxygenase 2;  rats; Sprague-Dawley;  animal experimentation
摘要:
目的:觀察獨活寄生湯含藥血清對白細胞介素1β(interleukin-1 beta,IL-1β)誘導(dǎo)的退變關(guān)節(jié)軟骨細胞中基質(zhì)金屬蛋白酶(matrix metalloproteinase,MMP)和環(huán)氧化酶2(cyclooxygenase 2,COX-2)表達的影響。方法:將36只2月齡SD大鼠隨機分為獨活寄生湯血清組、壯骨關(guān)節(jié)丸血清組和空白血清組,每組12只。分別以獨活寄生湯、壯骨關(guān)節(jié)丸溶液和生理鹽水灌胃,每次0.6 mL,每天2次,連續(xù)7 d。末次給藥后采血,分離血清備用。將體外培養(yǎng)的SD大鼠第3代關(guān)節(jié)軟骨細胞分為空白組、模型組、獨活寄生湯組和壯骨關(guān)節(jié)丸組。模型組、獨活寄生湯組和壯骨關(guān)節(jié)丸組軟骨細胞加入IL-1β干預(yù)24 h后,分別采用含10%空白血清組血清、獨活寄生湯血清組血清和壯骨關(guān)節(jié)丸血清組血清的DMEM培養(yǎng)基培養(yǎng); 空白組軟骨細胞不加IL-1β,直接以含10%空白血清組血清的DMEM培養(yǎng)基培養(yǎng)。培養(yǎng)72 h后采用Western Blot法測定各組細胞中MMP-1、MMP-3、MMP-9、MMP-13和COX-2的含量。結(jié)果:4組軟骨細胞的MMP-1、MMP-3、MMP-9、MMP-13和COX-2含量比較,組間差異均有統(tǒng)計學(xué)意義(0.135±0.007,0.324±0.006,0.174±0.007,0.234±0.007,F=266.333,P=0.000; 0.150±0.028,0.346±0.027,0.250±0.028,0.293±0.028,F=26.855,P=0.000; 0.131±0.014,0.283±0.009,0.148±0.014,0.158±0.015,F=50.442,P=0.000; 0.173±0.035,0.691±0.051,0.318±0.038,0.286±0.039,F=91.860,P=0.000; 0.201±0.033,0.796±0.031,0.370±0.033,0.327±0.034,F=125.270,P=0.000)。空白組的MMP-1、MMP-3、MMP-9、MMP-13和COX-2含量均低于模型組(P=0.000,P=0.000,P=0.000,P=0.000,P=0.000)。模型組的MMP-1、MMP-3、MMP-9、MMP-13和COX-2含量均高于獨活寄生湯組和壯骨關(guān)節(jié)丸組(P=0.000,P=0.003,P=0.000,P=0.000,P=0.000; P=0.000,P=0.047,P=0.000,P=0.000,P=0.000)。獨活寄生湯組的MMP-1含量低于壯骨關(guān)節(jié)丸組(P=0.000); 2組的MMP-3、MMP-9、MMP-13和COX-2含量比較,組間差異均無統(tǒng)計學(xué)意義(P=0.094,P=0.497,P=0.380,P=0.220)。結(jié)論:獨活寄生湯含藥血清可抑制IL-1β誘導(dǎo)的退變關(guān)節(jié)軟骨細胞中MMP-1、MMP-3、MMP-9、MMP-13和COX-2的表達,其抑制MMP-1表達的作用優(yōu)于壯骨關(guān)節(jié)丸含藥血清。
Abstract:
Objective:To observe the impact of Duhuo Jisheng Tang(獨活寄生湯,DHJST)medicated serum on expression of matrix metalloproteinase(MMP)and cyclooxygenase 2(COX-2)in degenerative articular chondrocytes induced by interleukin-1 beta(IL-1β).Methods:Thirty-six 2-month-old SD rats were randomly divided into DHJST serum group,Zhuanggu Guanjie Wan(壯骨關(guān)節(jié)丸, ZGGJW)serum group and blank serum group,12 cases in each group.The rats were intragastric administrated with DHJST,ZGGJW solution and normal saline respectively(0.6 mL at a time,twice a day)for 7 consecutive day.After the last dose,the blood was collected and the serum was separated for further use.The third-generation articular chondrocytes of SD rats cultured in vitro were divided into blank group,model group,DHJST group and ZGGJW group.The chondrocytes in the latter 3 groups were cultured in Dulbecco's Modified Eagle Medium(DMEM)supplemented with 10% serum of blank serum group,DHJST serum group and ZGGJW serum group after 24-hour intervention with IL-1β.The chondrocytes in blank group were cultured in DMEM supplemented with 10% serum of blank serum group and didn't intervented by IL-1β.The contents of MMP-1,MMP-3,MMP-9,MMP-13 and COX-2 in the chondrocytes of each group were measured by using Western Blot after 72-hour culture.Results:There was statistical difference in the contents of MMP-1,MMP-3,MMP-9,MMP-13 and COX-2 in the chondrocytes between the 4 groups(0.135+/-0.007,0.324+/-0.006,0.174+/-0.007,0.234+/-0.007,F=266.333,P=0.000; 0.150+/-0.028,0.346+/-0.027,0.250+/-0.028,0.293+/-0.028,F=26.855,P=0.000; 0.131+/-0.014,0.283+/-0.009,0.148+/-0.014,0.158+/-0.015,F=50.442,P=0.000; 0.173+/-0.035,0.691+/-0.051,0.318+/-0.038,0.286+/-0.039,F=91.860,P=0.000; 0.201+/-0.033,0.796+/-0.031,0.370+/-0.033,0.327+/-0.034,F=125.270,P=0.000).The contents of MMP-1,MMP-3,MMP-9,MMP-13 and COX-2 were lower in blank group compared to model group(P=0.000,P=0.000,P=0.000,P=0.000,P=0.000).The contents of MMP-1,MMP-3,MMP-9,MMP-13 and COX-2 were higher in model group compared to DHJST group and ZGGJW group(P=0.000,P=0.003,P=0.000,P=0.000,P=0.000; P=0.000,P=0.047,P=0.000,P=0.000,P=0.000).The content of MMP-1 was lower in DHJST group compared to ZGGJW group(P=0.000)and there was no statistical difference in the contents of MMP-3,MMP-9,MMP-13 and COX-2 between the 2 groups(P=0.094,P=0.497,P=0.380,P=0.220).Conclusion:DHJST medicated serum can inhibit the expression of MMP-1,MMP-3,MMP-9,MMP-13 and COX-2 in degenerative articular chondrocytes induced by IL-1β,and it surpasses ZGGJW medicated serum in inhibiting the expression of MMP-1.

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備注/Memo

備注/Memo:
2015-09-23收稿 2015-11-19修回
基金項目:福建省自然科學(xué)基金項目(2015J01338,2014J01357); 福建省中青年教師教育科研項目(JA14152); 陳可冀中西醫(yī)結(jié)合發(fā)展基金(CKJ2015010)
通訊作者:葉蕻芝 E-mail:[email protected]
更新日期/Last Update: 2015-12-30