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[1]趙佳盛,張?jiān)?桃紅四物湯治療兔早期筋膜間隔綜合征的 療效觀察及作用機(jī)制研究[J].中醫(yī)正骨,2015,27(04):1-7.
　ZHAO Jiasheng,ZHANG Yun.Experimental research on curative effect and mechanism of action of Taohong Siwu Tang(桃紅四物湯)for treatment of early compartment syndromes in rabbits[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):1-7.
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桃紅四物湯治療兔早期筋膜間隔綜合征的 療效觀察及作用機(jī)制研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期數(shù):
2015年04期
頁(yè)碼:
1-7
欄目:
基礎(chǔ)研究
出版日期:
2015-04-30

文章信息/Info

Title:
Experimental research on curative effect and mechanism of action of Taohong Siwu Tang(桃紅四物湯)for treatment of early compartment syndromes in rabbits
作者:
趙佳盛1; ZHAO Jiasheng1; ZHANG Yun2
1.Wenling Hospital of Traditional Chinese Medicine,Wenling 317502,Zhejiang,China
2.Luoyang Orthopedic-Traumatological Hospital,Luoyang 471002,Henan,China
關(guān)鍵詞:
筋膜間隔綜合征;  桃紅四物湯;  血液流變學(xué);  動(dòng)物實(shí)驗(yàn)
Keywords:
compartment syndromes;  Taohong Siwu Tang;  hemorheology;  animal experimentation
摘要:
目的:探討桃紅四物湯治療早期筋膜間隔綜合征的療效及作用機(jī)制。方法:采用氣壓止血帶壓迫法將20只成年雄性新西蘭大耳白兔制成后肢筋膜間隔綜合征模型,造模后將20只兔子隨機(jī)分為藥物組和模型組,每組10只。藥物組動(dòng)物以桃紅四物湯灌胃,每次7 mL; 模型組以等量生理鹽水灌胃; 每天2次,連續(xù)1周。分別于造模前、造模后12 h、1 d、2 d、4 d、7 d觀察動(dòng)物的一般情況、小腿腫脹度、脛前間室內(nèi)壓及血液流變學(xué)指標(biāo)。造模后7 d觀察完成之后處死所有動(dòng)物,取造模側(cè)小腿脛前肌及腓深神經(jīng)標(biāo)本在光鏡下檢查。結(jié)果:①一般情況。在實(shí)驗(yàn)過(guò)程中模型組動(dòng)物精神不振,飲食減少,大便正常,1只動(dòng)物死亡; 藥物組動(dòng)物精神、飲食尚可,大便稀溏,1只動(dòng)物死亡。②小腿腫脹度。造模前后不同時(shí)間造模側(cè)小腿腫脹度的差異有統(tǒng)計(jì)學(xué)意義,即存在時(shí)間效應(yīng)(F=244.260,P=0.000)。2組造模側(cè)小腿腫脹度比較總體上有統(tǒng)計(jì)學(xué)意義,即存在分組效應(yīng)(F=128.651,P=0.000); 除造模前和造模后12 h時(shí)外,其余各時(shí)點(diǎn)模型組的造模側(cè)小腿腫脹度均高于藥物組。時(shí)間因素與分組因素存在交互效應(yīng)(F=39.453,P=0.000)。③脛前間室內(nèi)壓。造模前后不同時(shí)間造模側(cè)脛前間室內(nèi)壓的差異有統(tǒng)計(jì)學(xué)意義,即存在時(shí)間效應(yīng)(F=317.971,P=0.000)。2組脛前間室內(nèi)壓比較總體上有統(tǒng)計(jì)學(xué)意義,即存在分組效應(yīng)(F=72.285,P=0.000); 除造模前和造模后12 h時(shí)外,其余各時(shí)點(diǎn)模型組的脛前間室內(nèi)壓均高于藥物組。時(shí)間因素與分組因素存在交互效應(yīng)(F=31.071,P=0.000)。④血液流變學(xué)指標(biāo)。造模前后不同時(shí)間全血黏度(200/S、30/S、5/S)的差異有統(tǒng)計(jì)學(xué)意義,即存在時(shí)間效應(yīng)(F=420.316,P=0.000; F=977.477,P=0.000; F=3 283.000,P=0.000)。2組全血黏度(200/S、30/S、5/S)比較總體上有統(tǒng)計(jì)學(xué)意義,即存在分組效應(yīng)(F=87.665,P=0.000; F=166.701,P=0.000; F=624.171,P=0.000); 除造模前和造模后12 h時(shí)外,其余各時(shí)點(diǎn)模型組的全血黏度(200/S、30/S、5/S)均高于藥物組。時(shí)間因素與分組因素存在交互效應(yīng)(F=87.611,P=0.000; F=204.266,P=0.000; F=744.771,P=0.000)。造模前后不同時(shí)間血漿黏度(plasma viscosity,ηp)的差異有統(tǒng)計(jì)學(xué)意義,即存在時(shí)間效應(yīng)(F=214.357,P=0.000)。2組ηp比較總體上有統(tǒng)計(jì)學(xué)意義,即存在分組效應(yīng)(F=14.874,P=0.000); 除造模前和造模后12 h時(shí)外,其余各時(shí)點(diǎn)模型組的ηp均高于藥物組。時(shí)間因素與分組因素存在交互效應(yīng)(F=70.651,P=0.000)。造模前后不同時(shí)間血細(xì)胞比容(hematocrit,HCT)的差異有統(tǒng)計(jì)學(xué)意義,即存在時(shí)間效應(yīng)(F=137.992,P=0.000)。2組HCT比較總體上有統(tǒng)計(jì)學(xué)意義,即存在分組效應(yīng)(F=21.696,P=0.000); 除造模前和造模后12 h時(shí)外,其余各時(shí)點(diǎn)模型組的HCT均高于藥物組。時(shí)間因素與分組因素存在交互效應(yīng)(F=109.939,P=0.000)。造模前后不同時(shí)間紅細(xì)胞沉降率(erythrocyte sedimentation rate,ESR)的差異有統(tǒng)計(jì)學(xué)意義,即存在時(shí)間效應(yīng)(F=71.738,P=0.000)。2組ESR比較總體上有統(tǒng)計(jì)學(xué)意義,即存在分組效應(yīng)(F=12.227,P=0.003); 除造模前和造模后12 h時(shí)外,其余各時(shí)點(diǎn)模型組的ESR均高于藥物組。時(shí)間因素與分組因素存在交互效應(yīng)(F=44.082,P=0.000)。⑤肌肉及神經(jīng)組織形態(tài)。模型組造模側(cè)脛前肌肌纖維嚴(yán)重萎縮、壞死,呈玻璃樣變性,橫紋模糊不清,組織間質(zhì)纖維化; 腓深神經(jīng)纖維變性、水腫,核固縮、減少或消失,神經(jīng)束膜增厚。藥物組造模側(cè)脛前肌肌纖維萎縮、橫紋模糊,組織間隙增寬,可見(jiàn)少量炎性細(xì)胞浸潤(rùn); 腓深神經(jīng)纖維水腫,少量變性,神經(jīng)束膜增厚。結(jié)論:桃紅四物湯對(duì)于早期筋膜間隔綜合征具有一定的防治作用,其作用機(jī)制可能是通過(guò)降低血液流變學(xué)指標(biāo),改善血液及微循環(huán)狀態(tài)。
Abstract:
Objective:To study the curative effect and mechanism of action of Taohong Siwu Tang(桃紅四物湯)for treatment of early compartment syndromes in rabbits.Methods:Twenty adult male big-eared New Zealand rabbits were made into compartment syndromes models in posterior limbs through compression with pneumatic tourniquet,and then the rabbits were randomly divided into drug group and model group,10 cases in each group.The rabbits in drug group were intragastric administrated with Taohong Siwu Tang(7 ml at a time); while the others in model group were intragastric administrated with the same dose of normal saline,twice a day for 1 consecutive week.The general state of health,degree of leg swelling,anterior compartment pressure and hemorheology indexes of rabbits were observed before the modeling and at 12 hrs and 1,2,4 and 7 days after the modeling respectively.All the rabbits were executed at 7 days after the modeling,and their anterior tibial muscles and deep peroneal nerves were fetched out and sectioned for inspection under the light microscope.Results:During the experiment,the rabbits in model group presented with depressed spirit,decreased eating and normal stool,and 1 rabbit died.The rabbits in drug group presented with normal spirit,normal eating and sloppy stool,and 1 rabbit died.There was statistical difference in leg swelling degree between different timepoints,in other words,there was time effect(F=244.260,P=0.000).There was statistical difference in leg swelling degree between the two groups in general,in other words,there was group effect(F=128.651,P=0.000).The leg swelling degree of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=39.453,P=0.000).There was statistical difference in anterior compartment pressure between different timepoints,in other words,there was time effect(F=317.971,P=0.000).There was statistical difference in anterior compartment pressure between the two groups in general,in other words,there was group effect(F=72.285,P=0.000).The anterior compartment pressure of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=31.071,P=0.000).There was statistical difference in blood viscosity(ηb)(200/S,30/S,5/S)between different timepoints,in other words,there was time effect(F=420.316,P=0.000; F=977.477,P=0.000; F=3 283.000,P=0.000).There was statistical difference in ηb(200/S,30/S,5/S)between the two groups in general,in other words,there was group effect(F=87.665,P=0.000; F=166.701,P=0.000; F=624.171,P=0.000).The ηb(200/S,30/S,5/S)of model group were higher than those of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=87.611,P=0.000; F=204.266,P=0.000; F=744.771,P=0.000).There was statistical difference in plasma viscosity(ηp)between different timepoints,in other words,there was time effect(F=214.357,P=0.000).There was statistical difference in ηp between the two groups in general,in other words,there was group effect(F=14.874,P=0.000).The ηp of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=70.651,P=0.000).There was statistical difference in hematocrit(HCT)between different timepoints,in other words,there was time effect(F=137.992,P=0.000).There was statistical difference in HCT between the two groups in general,in other words,there was group effect(F=21.696,P=0.000).The HCT of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=109.939,P=0.000).There was statistical difference in erythrocyte sedimentation rate(ESR)between different timepoints,in other words,there was time effect(F=71.738,P=0.000).There was statistical difference in ESR between the two groups in general,in other words,there was group effect(F=12.227,P=0.003).The ESR of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor(F=44.082,P=0.000).Severe atrophy,necrosis,hyaline degeneration,indefinite transverse striation and interstitial fibrosis were found in pretibial muscle fibers of rabbits in model group.Deep peroneal nerve fibres presented with(1)degeneration,(2)edema,(3)pycnosis,decrease or disappearance of nucleus and(4)thickened perineurium were also found in model group.Atrophy,indefinite transverse striation,dilated tissue spaces and a few inflammatory cells were found in pretibial muscle fibers of rabbits in drug group.Edema,a little degeneration and thickened perineurium in deep peroneal nerve fibres were also found in model group.Conclusion:Taohong Siwu Tang has preventive and therapeutic effect on early compartment syndromes.It can improve the state of blood and microcirculation through lowering the levels of hemorheology indexes,which may be the mechanisms of action for treatment of early compartment syndromes.

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備注/Memo:
2014-11-20收稿 2015-01-12修回
基金項(xiàng)目:河南省省屬科研單位社會(huì)公益項(xiàng)目預(yù)研專項(xiàng)資金項(xiàng)目
更新日期/Last Update: 2015-04-30