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[1]趙祚塨,王孝輝,姚俊娜,等.疏血通注射液預(yù)防大鼠血管吻合口血栓形成的實(shí)驗(yàn)研究[J].中醫(yī)正骨,2015,27(03):12-16.
　ZHAO Zuogong,WANG Xiaohui,YAO Junna,et al.An experimental study of Shuxuetong(疏血通)injection for prevention of vascular anastomotic stoma thrombosis in rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):12-16.
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疏血通注射液預(yù)防大鼠血管吻合口血栓形成的實(shí)驗(yàn)研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第27卷
期數(shù):
2015年03期
頁(yè)碼:
12-16
欄目:
基礎(chǔ)研究
出版日期:
2015-03-30

文章信息/Info

Title:
An experimental study of Shuxuetong(疏血通)injection for prevention of vascular anastomotic stoma thrombosis in rats
作者:
趙祚塨; 王孝輝; 姚俊娜; 郭紹勇; 陳利國(guó); 段衛(wèi)峰; 穆世民; 杜志軍; 蔡利濤
河南省洛陽(yáng)正骨醫(yī)院/河南省骨科醫(yī)院,河南 洛陽(yáng) 471002
Author(s):
ZHAO Zuogong; WANG Xiaohui; YAO Junna; GUO Shaoyong; Chen Liguo; DUAN Weifeng; MU Shimin; DU Zhijun; CAI Litao
Luoyang Orthopedic-Traumatological Hospital,Luoyang 471002,Henan,China
關(guān)鍵詞:
血栓形成;  疏血通;  肝素;  顯微外科手術(shù);  血小板;  纖維蛋白;  內(nèi)皮細(xì)胞;  動(dòng)物實(shí)驗(yàn)
Keywords:
thrombosis;  Shuxuetong;  heparin;  microsurgery;  blood platelets;  fibrin;  endothelial cells;  animal experimentation
摘要:
目的:觀察疏血通注射液預(yù)防大鼠血管吻合口血栓形成的療效。方法:將120只Wistar大鼠隨機(jī)分為對(duì)照組、疏血通組及肝素鈉組,每組40只。對(duì)照組、疏血通組及肝素鈉組大鼠分別以生理鹽水(10 mL·kg-1)、疏血通注射液(2 g·kg-1)及肝素鈉注射液(600 U·kg-1)進(jìn)行腹腔注射。藥物注射6 h后將所有大鼠左側(cè)頸總動(dòng)脈切斷,并即刻對(duì)其進(jìn)行端端吻合。分別于造模結(jié)束后30 min及1、3、7 d時(shí)從各組隨機(jī)選取10只大鼠,切取包含吻合口遠(yuǎn)近端各0.5 cm的頸總動(dòng)脈,在電子顯微鏡下觀察并計(jì)算吻合口及縫線表面覆蓋的纖維蛋白、血小板及內(nèi)皮細(xì)胞的百分比。結(jié)果:①纖維蛋白覆蓋率。造模后不同時(shí)間,3組大鼠血管吻合口纖維蛋白覆蓋率比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=133.802,P=0.000; F=18.741,P=0.000; F=212.943,P=0.000; F=341.174,P=0.000)。造模后30 min時(shí)對(duì)照組纖維蛋白覆蓋率低于疏血通組和肝素鈉組(P=0.000,P=0.000); 造模后1、3、7 d時(shí)對(duì)照組纖維蛋白覆蓋率均高于疏血通組和肝素鈉組(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000); 造模后3 d時(shí)疏血通組纖維蛋白覆蓋率高于肝素鈉組(P=0.000); 其余各時(shí)點(diǎn)疏血通組和肝素鈉組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義。②血小板覆蓋率。造模后不同時(shí)間,3組大鼠血管吻合口血小板覆蓋率比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=352.002,P=0.000; F=84.734,P=0.000; F=368.903,P=0.000; F=14.413,P=0.000)。造模后30 min、1 d、3 d、7 d時(shí)對(duì)照組血小板覆蓋率均高于疏血通組和肝素鈉組(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000); 造模結(jié)束后30 min時(shí)疏血通組血小板覆蓋率高于肝素鈉組(P=0.027); 其余各時(shí)點(diǎn)疏血通組和肝素鈉組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義。③內(nèi)皮細(xì)胞覆蓋率。除造模后30 min時(shí)外,其余各時(shí)點(diǎn)3組大鼠血管吻合口內(nèi)皮細(xì)胞覆蓋率比較,組間差異均有統(tǒng)計(jì)學(xué)意義(F=0.000,P=1.000; F=835.502,P=0.000; F=363.874,P=0.000; F=780.410,P=0.000)。造模后1、3、7 d時(shí)對(duì)照組內(nèi)皮細(xì)胞覆蓋率均低于疏血通組和肝素鈉組(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000); 造模后1、7 d時(shí)疏血通組內(nèi)皮細(xì)胞覆蓋率均高于肝素鈉組(P=0.002,P=0.001); 造模后3 d時(shí)疏血通組和肝素鈉組比較,組間差異無(wú)統(tǒng)計(jì)學(xué)意義。結(jié)論:疏血通注射液能促進(jìn)內(nèi)皮細(xì)胞生長(zhǎng),減少血小板在血管吻合口的堆積,從而避免血栓形成,其療效與肝素鈉相當(dāng)。
Abstract:
Objective:To observe the preventative effect of Shuxuetong(疏血通)injection on vascular anastomotic stoma thrombosis in rats.Methods:One hundred and twenty Wistar rats were randomly divided into control group,Shuxuetong group and heparin group,40 cases in each group.The Wistar rats in the 3 groups were intraperitoneal injected with normal saline(10 mL/kg),Shuxuetong injection(2 g/kg)and heparin injection(600 U/kg)respectively.The left common carotid arteries(LCCA)of all rats were cut off at 6 hours after injection,and end-to-end anastomosis were performed instantly.Ten rats were randomly selected from each group at 30 minutes,1,3 and 7 days after the modeling respectively,and the common carotid arteries near the anastomotic stoma were fetched for observing.The fraction of coverage of fibrin,blood platelets and endothelial cells at anastomotic stoma and suture surface were observed and calculated by using electron microscope.Results:There was statistical difference in coverage fraction of fibrin in vascular anastomotic stoma between the 3 groups after the modeling(F=133.802,P=0.000; F=18.741,P=0.000; F=212.943,P=0.000; F=341.174,P=0.000).The coverage fraction of fibrin of control group was lower than that of Shuxuetong group and heparin group at 30 minutes after the modeling(P=0.000,P=0.000).The coverage fraction of fibrin of control group was higher than that of Shuxuetong group and heparin group at 1,3 and 7 days after the- modeling(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000).The coverage fraction of fibrin of Shuxuetong group was higher than that of heparin group at 3 days after the modeling(P=0.000).There was no statistical difference in coverage fraction of fibrin between Shuxuetong group and heparin group at the rest timepoints.There was statistical difference in coverage fraction of blood platelets in vascular anastomotic stoma between the 3 groups after the modeling(F=352.002,P=0.000; F=84.734,P=0.000; F=368.903,P=0.000; F=14.413,P=0.000).The coverage fraction of blood platelets of control group was higher than that of Shuxuetong group and heparin group at 30 minutes and 1,3 and 7 days after the modeling(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000).The coverage fraction of blood platelets of Shuxuetong group was higher than that of heparin group at 30 minutes after the modeling(P=0.027).There was no statistical difference in coverage fraction of blood platelets between Shuxuetong group and heparin group at the rest timepoints.There was statistical difference in coverage fraction of endothelial cells in vascular anastomotic stoma between the 3 groups except at 30 minutes after the modeling(F=0.000,P=1.000; F=835.502,P=0.000; F=363.874,P=0.000; F=780.410,P=0.000).The coverage fraction of endothelial cells of control group was lower than that of Shuxuetong group and heparin group at 1,3 and 7 days after the modeling(P=0.000,P=0.000; P=0.000,P=0.000; P=0.000,P=0.000).The coverage fraction of endothelial cells of Shuxuetong group was higher than that of heparin group at 1 and 7 days after the modeling(P=0.002,P=0.001).There was no statistical difference in coverage fraction of endothelial cells between Shuxuetong group and heparin group at 3 days after the modeling.Conclusion:Shuxuetong injection can promote the growth of endothelial cells and reduce the accumulation of blood platelets in vascular anastomotic stoma to avoid thrombogenesis,and it is similar to heparin in the total curative effect.

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備注/Memo

備注/Memo:
2014-09-03收稿 2015-01-19修回
通訊作者:趙祚塨 E-mail:[email protected]
更新日期/Last Update: 2015-03-30