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[1]李長明,謝尚舉,全仁夫,等.電針秩邊穴與水道穴對大鼠急性脊髓損傷后 運動和排尿功能的影響[J].中醫(yī)正骨,2014,26(12):3-6.
　Li Changming*,Xie Shangju,Quan Renfu,et al.Effect of electroacupuncture Zhibian(BL54)and Shuidao(ST28)on motor function and urination function in rats with acute spinal cord injuries[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(12):3-6.
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電針秩邊穴與水道穴對大鼠急性脊髓損傷后 運動和排尿功能的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期數(shù):
2014年12期
頁碼:
3-6
欄目:
基礎研究
出版日期:
2014-12-30

文章信息/Info

Title:
Effect of electroacupuncture Zhibian(BL54)and Shuidao(ST28)on motor function and urination function in rats with acute spinal cord injuries
作者:
李長明1; 謝尚舉1; 全仁夫2; 楊宗保3
1.浙江中醫(yī)藥大學,浙江 杭州 310053;
2.浙江省杭州市蕭山區(qū)中醫(yī)院, 浙江 杭州 311201;
3.廈門大學醫(yī)學院,福建 廈門 361000
Author(s):
Li Changming*; Xie Shangju; Quan Renfu; Yang Zongbao.*
Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China
關鍵詞:
脊髓損傷 電針 穴; 秩邊 穴; 水道 運動功能 膀胱容量 剩余尿量 大鼠 動物實驗
Keywords:
Spinal cord injuries;  Electroacupuncture;  POINT BL54(ZHIBIAN);  POINT ST28(SHUIDAO);  Motor function;  Bladder capacity;  Residual urine volume;  Rats;  Animal experimentation
摘要:
目的:觀察電針秩邊穴與水道穴對大鼠急性脊髓損傷后運動和排尿功能的影響。方法:將48只SD大鼠隨機分為電針組和對 照組,每組24只。采用改良Allen's重物打擊法將所有大鼠制成急性脊髓損傷模型。造模后24 h,電針組大鼠在秩邊穴和水道穴進 行電針治療,對照組大鼠在秩邊穴和水道穴旁開0.5 cm處進行電針治療。每天治療1次,每次15 min。分別于實驗干預1 d、3 d、 7 d和14 d后采用BBB評分法評定2組大鼠的運動功能,同時于每次運動功能測試完成后,測定大鼠的膀胱容量和剩余尿量。結果: ①運動功能。干預后不同時間BBB評分的差異有統(tǒng)計學意義,即存在時間效應[(0.50±0.53)分,(2.90±1.10)分, (7.50±1.27)分,(11.50±0.71)分;(0.60±0.52)分,(2.70±0.95)分,(4.60±0.70)分, (8.60±0.97)分; F=625.000,P=0.000]。2組大鼠BBB評分的組間差異總體上有統(tǒng)計學意義,即存在分組效應 (F=29.866,P=0.000); 干預1 d、3 d后,2組大鼠的BBB評分比較,差異均無統(tǒng)計學意義(t=0.429,P=0.678; t=0.435,P=0.674); 干預7 d、14 d后,電針組大鼠的BBB評分均大于對照組(t=6.328,P=0.000; t=9.222,P=0.000)。時間因素和分組因素之間存在交 互效應(F=24.939,P=0.000)。②排尿功能。干預后不同時間膀胱容量的差異有統(tǒng)計學意義,即存在時間效應[(1.10±0.05) mL,(1.37±0.05)mL,(1.89±0.09)mL,(2.31±0.19)mL;(1.10±0.06)mL,(1.36±0.05)mL, (1.67±0.08)mL,(1.88±0.08)mL; F=193.885,P=0.000]。2組大鼠膀胱容量的組間差異總體上有統(tǒng)計學意義,即存在分 組效應(F=65.970,P=0.000); 干預1 d、3 d后,2組大鼠的膀胱容量比較,差異均無統(tǒng)計學意義(t=0.177,P=0.868; t=0.366,P=0.733); 干預7 d、14 d后,電針組大鼠的膀胱容量均大于對照組(t=4.186,P=0.014; t=4.647,P=0.009)。時間因素 和分組因素之間存在交互效應(F=10.283,P=0.000)。干預后不同時間剩余尿量的差異有統(tǒng)計學意義,即存在時間效應 [(2.00±0.20)mL,(1.88±0.15)mL,(1.49±0.07)mL,(0.98±0.11)mL;(1.99±0.19)mL, (1.89±0.17)mL,(1.73±0.12)mL,(1.29±0.07)mL; F=131.825,P=0.000]。2組大鼠剩余尿量的組間差異總體上有 統(tǒng)計學意義,即存在分組效應(F=3.787,P=0.038); 干預1 d、3 d后,2組大鼠的剩余尿量比較,差異均無統(tǒng)計學意義 (t=0.016,P=0.988; t=0.039,P=0.971); 干預7 d、14 d后,電針組大鼠的剩余尿量均小于對照組(t=3.791,P=0.019; t=5.334,P=0.006)。時間因素和分組因素之間存在交互效應(F=5.646,P=0.028)。結論:電針秩邊穴與水道穴能改善大鼠急性脊 髓損傷后的運動和排尿功能。
Abstract:
Objective:To observe the effect of electroacupuncture Zhibian(BL54)and Shuidao(ST28)on motor function and urination function in rats with acute spinal cord injuries.Methods:Forty-eight SD rats were randomly divided into electroacupuncture group and control group,24 cases in each group.The acute spinal cord injuries models were built by modified Allen's method in all rats.The rats in the electroacupuncture group were treated with electroacupuncture at Zhibian(BL54)and Shuidao(ST28),while the others in the control group were treated with electroacupuncture at points 0.5 cm apart from Zhibian(BL54)and Shuidao(ST28)at 24 hours after the modeling,once a day for 15 minutes at a time.The motor function were evaluated by using BBB score at 1st,3rd,7th and 14th days after experimental intervention.Meanwhile,the bladder capacity and residual urine volume of the rats were measured.Results:There was statistical difference in BBB scores between different time points after intervention,in other words,there was time effect(0.50+/-0.53,2.90+/- 1.10,7.50+/ 1.27,11.50+/-0.71 points; 0.60+/-0.52,2.70+/-0.95,4.60+/-0.70,8.60+/-0.97 points; F=625.000,P=0.000).In general,there was statistical difference in BBB scores between the 2 groups,in other words,there was grouping effect(F=29.866,P=0.000).There was no statistical difference in BBB scores between the 2 groups at 1 day and 3 days after intervention(t=0.429,P=0.678; t=0.435,P=0.674),and the BBB scores of the electroacupuncture group were greater than those of control group at 7 and 14 days after intervention(t=6.328,P=0.000; t=9.222,P=0.000).There was interaction between time factor and grouping factor(F=24.939,P=0.000).There was statistical difference in bladder capacity between different time points after intervention,in other words,there was time effect(1.10+/-0.05,1.37+/-0.05,1.89+/-0.09,2.31+/-0.19 mL; 1.10+/-0.06,1.36+/- 0.05,1.67+/-0.08,1.88+/-0.08 mL; F=193.885,P=0.000).In general,there was statistical difference in bladder capacity between the 2 groups,in other words,there was grouping effect(F=65.970,P=0.000).There was no statistical difference in bladder capacity between the 2 groups at 1 day and 3 days after intervention (t=0.177,P=0.868; t=0.366,P=0.733),and the bladder capacity of the electroacupuncture group was larger than that of control group at 7 and 14 days after intervention(t=4.186,P=0.014; t=4.647,P=0.009).There was interaction between time factor and grouping factor(F=10.283,P=0.000).There was statistical difference in residual urine volume between different time points after intervention,in other words,there was time effect (2.00+/-0.20,1.88+/-0.15,1.49+/-0.07,0.98+/-0.11 mL; 1.99+/-0.19,1.89+/-0.17,1.73+/-0.12,1.29+/-0.07 mL; F=131.825,P=0.000).In general,there was statistical difference in residual urine volume between the 2 groups,in other words,there was grouping effect(F=3.787,P=0.038).There was no statistical difference in residual urine volume between the 2 groups at 1 day and 3 days after intervention(t=0.016,P=0.988; t=0.039,P=0.971),and the residual urine volume of the electroacupuncture group was smaller than that of control group at 7 and 14 days after intervention(t=3.791,P=0.019; t=5.334,P=0.006).There was interaction between time factor and grouping factor(F=5.646,P=0.028).Conclusion:The motor function and urination function of rats with acute spinal cord injuries can be improved by electroacupuncture Zhibian(BL54)and Shuidao(ST28).

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備注/Memo

備注/Memo:
基金項目:浙江省中醫(yī)藥科研計劃項目(2008CB067)
通訊作者:全仁夫 E-mail:[email protected]
更新日期/Last Update: 2014-12-30