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[1]熊焱昊,王普申,齊進(jìn),等.中藥外敷超微粉體對(duì)大鼠骨折后骨痂形成和改建的影響[J].中醫(yī)正骨,2014,26(11):20-25.
　Xiong Yanhao*,Wang Pushen,Qi Jin,et al.Effect of traditional Chinese drug ultramicro-powder for external application on the formation and reconstruction of bony callus after fracture in rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(11):20-25.
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中藥外敷超微粉體對(duì)大鼠骨折后骨痂形成和改建的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期數(shù):
2014年11期
頁(yè)碼:
20-25
欄目:
基礎(chǔ)研究
出版日期:
2014-11-30

文章信息/Info

Title:
Effect of traditional Chinese drug ultramicro-powder for external application on the formation and reconstruction of bony callus after fracture in rats
作者:
熊焱昊1; 王普申1; 齊進(jìn)1; 劉志安2
1.上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院,上海 200025;
2.浙江省舟山廣安骨傷醫(yī)院,浙江 舟山 316102
Author(s):
Xiong Yanhao*; Wang Pushen; Qi Jin; Liu Zhian.*
Rui Jin Hospital Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China
關(guān)鍵詞:
骨痂 超微粉體 中藥外敷 散劑 動(dòng)物實(shí)驗(yàn)
Keywords:
Bony callus;  Ultramicro-powder;  External applications(TCD);  Pulvers;  Animal experimentation
摘要:
目的:觀察中藥外敷超微粉體對(duì)大鼠骨折后骨痂形成和改建的影響。方法:將60只3月齡SD雌性大鼠制成左側(cè)脛骨骨折模型 ,并以克氏針固定。造模結(jié)束后將大鼠隨機(jī)分為超微組、散劑組和對(duì)照組,每組20只。自術(shù)后第1天開(kāi)始,超微組和散劑組大鼠分 別采用中藥外敷超微粉體和中藥外敷散劑外敷骨折部位,每天2次,共28 d,對(duì)照組不進(jìn)行干預(yù)。分別于術(shù)后7 d、14 d、21 d和28 d每組各處死5只大鼠,取左側(cè)脛骨分別進(jìn)行X線、組織學(xué)及Micro-CT檢查。結(jié)果:①X線檢查結(jié)果。超微組和散劑組術(shù)后28 d時(shí)骨 折處外骨痂密度增加,骨折線模糊,均接近愈合,其中超微組大鼠骨折處外骨痂更致密; 對(duì)照組術(shù)后28 d時(shí)仍可看到骨折線。②組 織學(xué)觀察結(jié)果。術(shù)后7 d骨折處主要為纖維骨痂,3組無(wú)明顯差別; 術(shù)后14 d和21 d時(shí),軟骨骨痂開(kāi)始取代纖維性骨痂; 術(shù)后28 d 時(shí)軟骨骨痂周圍不斷有原始骨小梁出現(xiàn),骨折斷端表面出現(xiàn)破骨細(xì)胞,原始骨小梁不斷改建成為成熟骨小梁,其中以超微組骨小梁 最多。③Micro-CT檢查結(jié)果。術(shù)后7 d時(shí)Micro-CT檢查,3組均未見(jiàn)到明顯骨痂。術(shù)后14 d時(shí)3組大鼠骨折部位骨痂體積和骨痂密 度組間比較,差異均有統(tǒng)計(jì)學(xué)意義[(20.2±2.3)mm3,(16.4±1.7)mm3,(14.4±1.3) mm3,F=13.286,P=0.001;(184.6±9.3)mg·m-3,(162.8±5.7)mg·m-3, (148.2±6.8)mg·m-3,F=30.649,P=0.001]; 超微組的骨痂體積和密度均高于散劑組和對(duì)照組 (P=0.000,P=0.000; P=0.000,P=0.000),散劑組骨痂體積和密度均高于對(duì)照組(P=0.001,P=0.001)。術(shù)后21 d時(shí)3組大鼠骨折部位 骨痂體積和骨痂密度組間比較,差異均有統(tǒng)計(jì)學(xué)意義[(25.6±3.6)mm3,(21.2±0.8)mm3, (17.6±1.8)mm3,F=41.517,P=0.000;(248.2±16.0)mg·m-3,(222.6±7.8)mg·m- 3,(197.8±9.0)mg·m-3,F=23.834,P=0.000]; 超微組的骨痂體積和密度均高于散劑組和對(duì)照組 (P=0.000,P=0.000; P=0.000,P=0.000),散劑組骨痂體積和密度均高于對(duì)照組(P=0.000,P=0.000)。術(shù)后28 d時(shí)3組大鼠骨折部位 骨痂體積和骨痂密度組間比較,差異均有統(tǒng)計(jì)學(xué)意義[(30.2±0.8)mm3,(23.4±1.1)mm3, (21.4±1.1)mm3,F=96.727,P=0.000;(292.2±10.9)mg·m-3,(256.8±6.5)mg·m- 3,(222.8±4.4)mg·m-3,F=98.826,P=0.000]; 超微組的骨痂體積和密度均高于散劑組和對(duì)照組 (P=0.000,P=0.000; P=0.000,P=0.000),散劑組骨痂體積和密度均高于對(duì)照組(P=0.000,P=0.000)。結(jié)論:中藥外敷超微粉體能夠 促進(jìn)大鼠骨折后軟骨骨痂向骨性骨痂轉(zhuǎn)化,提高骨痂密度,促進(jìn)骨折愈合,其效果優(yōu)于中藥外敷散劑。
Abstract:
Objective:To observe the effect of traditional Chinese drug(TCD)ultramicro-powder for external application on the formation and reconstruction of bony callus after fracture in rats.Methods:Sixty 3-month-old female SD rats were made into the left tibia fracture models and the fractures were fixed with kirschner wires.Then the rats were randomly divided into ultramicro-powder group,pulvers group and control group,20 cases in each group.The rats in ultramicro-powder group and pulvers group were treated with TCD ultramicro-powder for external application and pulvers for external application at fracture sites respectively on the first day after operation,twice a day for 28 consecutive days,while rats in the control group were not be treated.At 7,14,21 and 28 days after the operation,5 rats were selected from each group and were executed,and their left tibiaes were fetched out for X-ray examination,histology examination and micro-CT examination.Results:At the 28th day after operation,the density of external callus increased at fracture site of ultramicro-powder group and pulvers group,moreover,the fracture lines were fuzzy and the fracture approached clinic healing.The density of external callus of ultramicro-powder group was more dense,while the fracture line can still be seen in control group at the 28th day after operation.At the 7th day after operation,the fiber calluses were found at fracture sites and there was no obvious difference between the 3 groups.At the 14th and 21th day after operation,the fiber calluses began to be replaced by cartilage callus.At the 28th day after operation,original bone trabeculas appeared constantly around the cartilage callus,and the osteoclasts were also found on fracture sites.The original bone trabeculas converted to mature bone trabeculas constantly,and the bone trabeculas were the most in ultramicro-powder group.The micro-CT examination showed that no visible bony callus were found in the 3 groups at the 7th day after operation.There were statistical difference in volume and density of bony callus at fracture sites between the 3 groups at the 14th day after operation(20.2+/-2.3,16.4+/-1.7,14.4+/-1.3 mm(3),F=13.286,P=0.001; 184.6+/-9.3,162.8+/-5.7,148.2+/-6.8 mg/m(3),F=30.649,P=0.001).The volume and density of bony callus were higher in ultramicro-powder group compared with pulvers group and control group(P=0.000,P=0.000; P=0.000,P=0.000),and were higher in pulvers group compared with control group(P=0.001,P=0.001).There were statistical difference in volume and density of bony callus at fracture sites between the 3 groups at the 21th day after operation (25.6+/-3.6,21.2+/-0.8,17.6+/-1.8 mm(3),F=41.517,P=0.000; 248.2+/-16.0,222.6+/-7.8,197.8+/-9.0 mg/m (3),F=23.834,P=0.000).The volume and density of bony callus were higher in ultramicro-powder group compared with pulvers group and control group(P=0.000,P=0.000; P=0.000,P=0.000),and were higher in pulvers group compared with control group(P=0.000,P=0.000).There were statistical difference in volume and density of bony callus at fracture sites between the 3 groups at the 28th day after operation(30.2+/-0.8,23.4+/-1.1,21.4+/-1.1 mm(3),F=96.727,P=0.000; 292.2+/-10.9,256.8+/-6.5,222.8+/-4.4 mg/m(3),F=98.826,P=0.000).The volume and density of bony callus were higher in ultramicro-powder group compared with pulvers group and control group (P=0.000,P=0.000; P=0.000,P=0.000),and were higher in pulvers group compared with control group (P=0.000,P=0.000).Conclusion:The TCD ultramicro-powder for external application can promote the transformation of cartilage callus into osseous callus in rats after fracture and raise the bony callus density.Therefore,it can promote fracture healing.Moreover,its therapeutic effect is better than that of TCD pulvers for external application.

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備注/Memo

備注/Memo:
基金項(xiàng)目:浙江省舟山市醫(yī)藥衛(wèi)生科技計(jì)劃項(xiàng)目(2012B28)
通訊作者:劉志安 E-mail:[email protected]
更新日期/Last Update: 2014-11-30