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[1]李峰,郭艷幸,曹向陽,等.貫葉連翹灌胃聯(lián)合光動力療法對類風(fēng)濕關(guān)節(jié)炎白兔膝關(guān)節(jié)滑膜組織病理形態(tài)的影響[J].中醫(yī)正骨,2014,26(09):6-10.
　Li Feng*,Guo Yanxing,Cao Xiangyang,et al.Effect of intragastric administration of hypericum perforatum combined with photodynamic therapy on the pathological morphology of synovial tissue of knee joints in rabbits with rheumatoid arthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(09):6-10.
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貫葉連翹灌胃聯(lián)合光動力療法對類風(fēng)濕關(guān)節(jié)炎白兔膝關(guān)節(jié)滑膜 組織病理形態(tài)的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期數(shù):
2014年09期
頁碼:
6-10
欄目:
基礎(chǔ)研究
出版日期:
2014-09-30

文章信息/Info

Title:
Effect of intragastric administration of hypericum perforatum combined with photodynamic therapy on the pathological morphology of synovial tissue of knee joints in rabbits with rheumatoid arthritis
作者:
李峰1; 郭艷幸1; 曹向陽1; 朱小磊1; 李沛2
1.河南省洛陽正骨醫(yī)院,河南 洛陽 471002;
2.河南省中醫(yī)院,河南 鄭州 450002
Author(s):
Li Feng*; Guo Yanxing; Cao Xiangyang; Zhu Xiaolei; Li Pei.
*Luoyang Orthopedic-Traumatological Hospital,Luoyang 471002,Henan,China
關(guān)鍵詞:
關(guān)節(jié)炎; 類風(fēng)濕 光化學(xué)療法 貫葉連翹 金絲桃素 病理形態(tài)學(xué) 兔 動物實驗
Keywords:
Arthritis; rheumatoid;  Photochemotherapy;  Hypericum perforatum;  Hypericin;  Pathomorphism;  Rabbits;  Animal experimentation
摘要:
目的:觀察貫葉連翹灌胃聯(lián)合光動力療法對類風(fēng)濕關(guān)節(jié)炎白兔膝關(guān)節(jié)滑膜組織病理形態(tài)的影響。方法:將36只12周齡新西蘭大白兔隨機分為正常對照組(空白組)12只、模型組12只、治療組12只。模型組和治療組用雞卵蛋白誘導(dǎo)建立類風(fēng)濕關(guān)節(jié)炎模型。造模后1周,治療組以1 500 mg·mL-1貫葉連翹藥液灌胃,空白組和模型組以生理鹽水灌胃,每天1次,連續(xù)灌胃7 d。隨機選取治療組大白兔一側(cè)膝關(guān)節(jié)作為貫葉連翹聯(lián)合光動力療法組,另一側(cè)膝關(guān)節(jié)作為貫葉連翹組。貫葉連翹聯(lián)合光動力療法組于每次灌胃后2 h采用氦氖激光治療儀照射膝關(guān)節(jié),每天1次,連續(xù)照射7 d; 貫葉連翹組膝關(guān)節(jié)避光。治療結(jié)束后2周,處死白兔,取各組白兔膝關(guān)節(jié)并分離出滑膜組織,采用HE染色法觀察膝關(guān)節(jié)滑膜的組織形態(tài)學(xué)變化,并按照滑膜病理5級評分法對炎性細(xì)胞浸潤、滑膜細(xì)胞增生及滑膜纖維組織增生3項指標(biāo)進(jìn)行評分。結(jié)果:①膝關(guān)節(jié)滑膜形態(tài)觀察。空白組滑膜襯里層細(xì)胞多呈單層,排列規(guī)則,滑膜組織較為疏松,無炎性細(xì)胞浸潤; 貫葉連翹聯(lián)合光動力療法組滑膜細(xì)胞增生層數(shù)較少,炎性細(xì)胞浸潤程度及滑膜纖維組織增生程度較模型組及貫葉連翹組明顯減輕。②膝關(guān)節(jié)滑膜病理指標(biāo)評分。各組滑膜炎性細(xì)胞浸潤評分比較,差異有統(tǒng)計學(xué)意義(F=30.130,P=0.000)。組間兩兩比較,空白組評分[(0.50±0.36)分]低于模型組[(2.92±1.00)分]、貫葉連翹組[(2.67±0.65)分]和貫葉連翹聯(lián)合光動力療法組[(1.58±0.51)分] [q=11.973,P=0.000; q=10.735,P=0.000; q=5.365,P=0.000]; 模型組評分高于貫葉連翹聯(lián)合光動力療法組(q=6.603,P=0.000); 模型組評分與貫葉連翹組比較,差異無統(tǒng)計學(xué)意義(q=1.238,P=0.386); 貫葉連翹聯(lián)合光動力療法組評分低于貫葉連翹組(q=5.365,P=0.000)。各組滑膜細(xì)胞增生評分比較,差異有統(tǒng)計學(xué)意義(F=30.910,P=0.000)。組間兩兩比較,空白組評分[(0.92±0.67)分]低于模型組[(3.33±0.78)分]、貫葉連翹組[(2.92±0.67)分]和貫葉連翹聯(lián)合光動力療法組[(1.75±0.62)分](q=12.187,P=0.000; q=10.084,P=0.000; q=4.200,P=0.005); 模型組評分高于貫葉連翹聯(lián)合光動力療法組(q=7.982,P=0.000); 模型組評分與貫葉連翹組比較,差異無統(tǒng)計學(xué)意義(q=2.103,P=0.144); 貫葉連翹聯(lián)合光動力療法組評分低于貫葉連翹組(q=5.884,P=0.000)。各組滑膜纖維組織增生評分比較,差異有統(tǒng)計學(xué)意義(F=25.700,P=0.000)。組間兩兩比較,空白組評分[(0.75±0.62)分]低于模型組[(2.83±0.83)分]、貫葉連翹組[(2.67±0.49)分]和貫葉連翹聯(lián)合光動力療法組[(1.67±0.65)分](q=10.903,P=0.000; q=10.034,P=0.000; q=4.780,P=0.001); 模型組評分高于貫葉連翹聯(lián)合光動力療法組(q=6.108,P=0.000); 模型組評分與貫葉連翹組比較,差異無統(tǒng)計學(xué)意義(q=0.874,P=0.540); 貫葉連翹聯(lián)合光動力療法組評分低于貫葉連翹組(q=5.234,P=0.001)。結(jié)論:貫葉連翹灌胃聯(lián)合光動力療法能有效地抑制類風(fēng)濕關(guān)節(jié)炎白兔膝關(guān)節(jié)炎性滑膜細(xì)胞浸潤,減輕其滑膜細(xì)胞增生和滑膜纖維組織增生,但其具體作用機制尚不明確,有待進(jìn)一步研究。
Abstract:
Objective:To study the effect of intragastric administration of hypericum perforatum combined with photodynamic therapy on the pathological morphology of synovial tissue of knee joints in rabbits with rheumatoid arthritis.Methods:Thirty-six 12-week-old New Zealand white rabbits were randomly divided into 3 groups,12 cases in normal control group(blank group),12 cases in model group,and 12 cases in treatment group.Rabbits in model group and treatment group were induced into the models of rheumatoid arthritis by using chicken ovalbumin.Rabbits in treatment group were intragastric administrated with the liquor of hyperforin perforatum with dose of 1 500 mg/mL,while rabbits in blank group and model group were intragastric administrated with normal saline 1 week after modeling.Rabbits in each group were intragastric administrated once a day for consecutive 7 days.Unilateral knee joints of these rabbits in treatment group were randomly recruited into hyperforin perforatum combined with photodynamic therapy group(combination therapy group),and the others were recruited into hyperforin perforatum group.The knee joints of former group were treated with He-Ne laser irradiation 2 hours after intragastric administration once a day for consecutive 7 days,while the knee joints of latter group were protected from light.These rabbits were executed and the knee joints were fetched out for separating synovial tissues 2 weeks after the end of treatment.The morphological changes of synovial tissues in knee joints were observed after HE staining; and inflammatory cell infiltration,synovial cell hyperplasia and synovial fibroplasia were assessed according to the synovial pathological scores.Results:Synovial lining cells spread in monolayer regularly in blank group,and the synovial tissue was relative sparsate and no inflammatory cells infiltrating were found.Less layer of synovial cells hyperplasia was found in combination therapy group,and the degree of inflammatory cell infiltration and synovial fibroplasia were alleviated significantly,compared to model group and hyperforin perforatum group.There were statistical differences in the scores of synovial inflammatory cell infiltration between the groups(F=30.130,P=0.000).Further pairwise comparison showed that the scores of blank group(0.50+/-0.36 points)were lower than those of model group(2.92+/-1.00 points),hyperforin perforatum group(2.67+/-0.65 points)and combination therapy group(1.58+/-0.51 points)(q=11.973,P=0.000; q=10.735,P=0.000; q=5.365,P=0.000); Model group surpassed combination therapy group in scores(q=6.603,P=0.000); There were no statistical differences in the scores between model group and hyperforin perforatum group(q=1.238,P=0.386); The scores of combination therapy group were lower than those of hyperforin perforatum group(q=5.365,P=0.000).There were statistical differences in the scores of synovial cell hyperplasia between the groups(F=30.910,P=0.000).Further pairwise comparison showed that the scores of blank group(0.92+/-0.67 points)were lower than those of model group(3.33+/-0.78 points),hyperforin perforatum group(2.92+/-0.67 points)and combination therapy group(1.75+/-0.62 points)(q=12.187,P=0.000; q=10.084,P=0.000; q=4.200,P=0.005).Model group surpassed combination therapy group in scores(q=7.982,P=0.000); There were no statistical differences in the scores between model group and hyperforin perforatum group(q=2.103,P=0.144); The scores of combination therapy group were lower than those of hyperforin perforatum group(q=5.884,P=0.000).There were statistical differences in the scores of synovial fibroplasia between the groups(F=25.700,P=0.000).Further pairwise comparison showed that the scores of blank group(0.75+/-0.62 points)were lower than those of model group(2.83+/-0.83 points),hyperforin perforatum group(2.67+/-0.49 points),and combination therapy group(1.67+/-0.65 points)(q=10.903,P=0.000; q=10.034,P=0.000; q=4.780,P=0.001).Model group surpassed combination therapy group(q=6.108,P=0.000); There were no statistical differences in the scores between model group and hyperforin perforatum group(q=0.874,P=0.540).The scores of combination therapy group were lower than those of hyperforin perforatum group(q=5.234,P=0.001).Conclusion:Intragastric administration of hyperforin perforatum combined with photodynamic therapy can effectively inhibit inflammatory cell infiltration and reduce synovial cell hyperplasia and synovial fibroplasia in knee joints of rabbits with rheumatoid arthritis,but its specific mechanism of action is unclear and need to be further studied.

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通訊作者:郭艷幸 E-mail:[email protected]
更新日期/Last Update: 2014-09-30