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[1]吳權(quán),李啟運(yùn),王素英,等.活血、溫經(jīng)、補(bǔ)益肝腎類中藥對膝骨關(guān)節(jié)炎兔關(guān)節(jié)軟骨形態(tài)的影響[J].中醫(yī)正骨,2014,26(06):15-18.
　Wu Quan*,Li Qiyun,Wang Suying,et al.Effect of three kinds of traditional Chinese drug including activating blood agents,warming meridians agents and reinforcing liver&kidney agents on articular cartilage morphology in rabbits with knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(06):15-18.
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活血、溫經(jīng)、補(bǔ)益肝腎類中藥對膝骨關(guān)節(jié)炎兔關(guān)節(jié)軟骨形態(tài)的 影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期數(shù):
2014年06期
頁碼:
15-18
欄目:
基礎(chǔ)研究
出版日期:
2014-06-30

文章信息/Info

Title:
Effect of three kinds of traditional Chinese drug including activating blood agents,warming meridians agents and reinforcing liver&kidney agents on articular cartilage morphology in rabbits with knee osteoarthritis
作者:
吳權(quán)1; 李啟運(yùn)1; 王素英2; 龍樹明1; 柯高峰3
1.浙江省寧波市中醫(yī)院,浙江 寧波 315010; 2.浙江省寧波市病理中心, 浙江 寧波 315031; 3.浙江中醫(yī)藥大學(xué),浙江 杭州 310053
Author(s):
Wu Quan*; Li Qiyun; Wang Suying; Long Shuming; Ke Gaofeng.*
Ningbo Hospital of Traditional Chinese Medicine,Ningbo 315010,Zhejiang,China
關(guān)鍵詞:
骨關(guān)節(jié)炎; 膝 軟骨; 關(guān)節(jié) 活血 溫經(jīng) 補(bǔ)益肝腎 動物實(shí)驗(yàn)
Keywords:
Osteoarthritis; knee;  Cartilage; articular;  Activating blood;  Warming meridians;  Reinforcing liver&; kidney;  Animal experimentation
摘要:
目的:觀察活血、溫經(jīng)及補(bǔ)益肝腎3類中藥對膝骨關(guān)節(jié)炎兔關(guān)節(jié)軟骨形態(tài)的影響。方法:將62只新西蘭白兔隨機(jī)分為6組,A組12只,B、C、D、E和F組各10只。采用關(guān)節(jié)制動法對B、C、D、E和F組動物左后腿進(jìn)行膝骨關(guān)節(jié)炎造模。造模成功后進(jìn)行藥物干預(yù),A、B組以10 mL蒸餾水灌胃,C組以10 mL丹參和當(dāng)歸藥液灌胃,D組以10 mL桂枝和防風(fēng)藥液灌胃,E組以10 mL杜仲和懷牛膝藥液灌胃,F組以10 mL金烏骨通膠囊水溶液灌胃。各組動物均在每天上午10時給藥1次。分別于藥物干預(yù)21 d和42 d后分2批處死動物,取左后腿股骨外髁關(guān)節(jié)軟骨及軟骨下骨,制成組織切片后分別進(jìn)行HE染色和阿利辛藍(lán)-過碘酸雪夫氏染色,采用Mankin軟骨組織學(xué)評分法進(jìn)行評分。結(jié)果:①動物一般情況。造模結(jié)束時,B、C、D、E和F組動物膝關(guān)節(jié)腫脹,屈伸活動不利,行走呈跨越步態(tài)。造模期間共有9只動物因拒絕進(jìn)食死亡。②Mankin評分。藥物干預(yù)后21 d,各組動物膝關(guān)節(jié)軟骨Mankin評分比較,差異有統(tǒng)計(jì)學(xué)意義[(0.67±0.82)分,(13.00±1.63)分,(4.75±2.22)分,(6.75±0.96)分,(8.25±1.26)分,(9.75±2.36)分,F=34.904,P=0.000]。組間兩兩比較,A組評分小于B、C、D、E和F組(P=0.000,P=0.000,P=0.000,P=0.000,P=0.009); B組大于C、D、E和F組(P=0.001,P=0.000,P=0.000,P=0.000); C組小于E組和F組(P=0.005,P=0.000); D組小于F組(P=0.015); 其余各組間兩兩比較,差異均無統(tǒng)計(jì)學(xué)意義。藥物干預(yù)后42 d,各組動物膝關(guān)節(jié)軟骨Mankin評分比較,差異有統(tǒng)計(jì)學(xué)意義[(0.67±1.03)分,(13.25±1.50)分,(3.00±0.81)分,(4.50±1.73)分,(4.00±1.10)分,(4.75±1.71)分,F=44.868,P=0.000]。組間兩兩比較,A組評分小于B、C、D、E和F組(P=0.000,P=0.014,P=0.000,P=0.001,P=0.000); B組大于C、D、E和F組(P=0.000,P=0.000,P=0.000,P=0.000); 其余各組間兩兩比較,差異均無統(tǒng)計(jì)學(xué)意義。E組和F組干預(yù)后42 d的膝關(guān)節(jié)軟骨Mankin評分小于干預(yù)后21 d的膝關(guān)節(jié)軟骨Mankin評分(t=5.112,P=0.001; t=3.532,P=0.017),其余各組藥物干預(yù)后21 d和42 d膝關(guān)節(jié)軟骨Mankin評分比較,差異均無統(tǒng)計(jì)學(xué)意義。結(jié)論:活血、溫經(jīng)及補(bǔ)益肝腎3類中藥均能改善膝骨關(guān)節(jié)炎兔膝關(guān)節(jié)軟骨形態(tài),達(dá)到保護(hù)軟骨、延緩軟骨退變的目的,但不能阻斷和逆轉(zhuǎn)軟骨退變的進(jìn)程。
Abstract:
Objective:To observe the effect of three kinds of traditional Chinese drug(TCD)including activating blood agents,warming meridians agents and reinforcing liver&kidney agents on articular cartilage morphology in rabbits with knee osteoarthritis(KOA).Methods:Sixty-two New Zealand white rabbits were randomly divided into 6 groups,12 cases in group A and 10 cases in each of the rest groups.The rabbits in group B,C,D,E and F were administrated with immobilization in the left hind legs to build models of KOA.Then,the rabbits in group A and B were intragastric administrated with distilled water(10 mL),while the rabbits in group C,D,E and F were intragastric administrated with solution of salvia miltiorrhiza and angelica(10mL),solution of cassia twig and saposhnikovia divaricata(10 mL),solution of eucommia ulmoides and bidentata blume(10 mL)and JINWU GUTONG solution(10 mL)respectively.The rabbits in each group were dosed with drug once a day at 10 am.The rabbits were executed in 2 batches at 3 and 6 weeks after the beginning of the drug intervention,and their lateral condyle articulars cartilago and subchondral bone in the left hind leg were fetched out.Their histologic sections were received HE staining and Alcian blue-periodic acid Schiff staining respectively and were evaluated according to Mankin cartilage histological score.Results:After the modeling,the knee of the rabbits in group B,C,D,E and F were swollen and were unfavourable for flexion.The rabbits showed steppage gait.Nine rabbits died of hunger strike during the modeling period.There was statistical difference in Mankin scores between the 6 groups at 21 days after the beginning of the drug intervention(0.67+/-0.82,13.00+/-1.63,4.75+/-2.22,6.75+/-0.96,8.25+/-1.26,9.75+/-2.36 points,F=34.904,P=0.000).Further pairwise comparison showed that the score of group A was less than those of group B,C,D,E and F(P=0.000,P=0.000,P=0.000,P=0.000,P=0.009),the score of group B was greater than those of group C,D,E and F(P=0.001,P=0.000,P=0.000,P=0.000),the score of group C was less than those of group E and F(P=0.005,P=0.000),and the score of group D was less than that of group F(P=0.015),while there was no statistical difference in Mankin scores between the rest paired groups.There was statistical difference in Mankin scores between the 6 groups at 42 days after the beginning of the drug intervention(0.67+/-1.03,13.25+/-1.50,3.00+/-0.81,4.50+/-1.73,4.00+/-1.10,4.75+/-1.71 points,F=44.868,P=0.000).Further pairwise comparison showed that the score of group A was less than those of group B,C,D,E and F(P=0.000,P=0.014,P=0.000,P=0.001,P=0.000),the score of group B was greater than those of group C,D,E and F(P=0.000,P=0.000,P=0.000,P=0.000),while there was no statistical difference in the scores between the rest paired groups.The Mankin scores of group E and F were less at 42 days compared to 21 days after the beginning of the drug intervention(t=5.112,P=0.001; t=3.532,P=0.017),while there was no statistical difference in the scores between the two time points for the other groups.Conclusion:All the three kinds of TCD,including activating blood agents,warming meridians agents and reinforcing liver&kidney agents,can ameliorate articular cartilage morphology in rabbits with KOA,so as to protect the cartilage and delay cartilage degeneration.However,it can't interdict and reverse the process of cartilage degeneration.

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更新日期/Last Update: 1900-01-01