84年鼠女哪年财运最旺,857comvvv色九欧美激情|85PO_87国产精品欲av国产av资源

[1]伍海昭,朱敏,詹紅生,等.骨碎補(bǔ)總黃酮對(duì)去卵巢大鼠骨組織Ⅰ型膠原表達(dá)及骨代謝的影響[J].中醫(yī)正骨,2014,26(04):11-14.
　Wu Haizhao*,Zhu Min,Zhan Hongsheng,et al.Effect of drynaria fortunei total flavonoids on collagen typeⅠexpression in bone tissue and bone metabolism in the ovariectomized rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(04):11-14.
點(diǎn)擊復(fù)制

骨碎補(bǔ)總黃酮對(duì)去卵巢大鼠骨組織Ⅰ型膠原表達(dá)及骨代謝的 影響()
分享到：

《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期數(shù):
2014年04期
頁(yè)碼:
11-14
欄目:
基礎(chǔ)研究
出版日期:
2014-04-30

文章信息/Info

Title:
Effect of drynaria fortunei total flavonoids on collagen typeⅠexpression in bone tissue and bone metabolism in the ovariectomized rats
作者:
伍海昭1; 朱敏1; 詹紅生2; 陳海嘯1
1.浙江省臺(tái)州醫(yī)院,浙江 臨海 317000; 2.上海中醫(yī)藥大學(xué)附屬曙光醫(yī)院,上海 201203
Author(s):
Wu Haizhao*; Zhu Min; Zhan Hongsheng; Chen Haixiao.*
The Taizhou hospital of Zhejiang province,Linhai 317000,Zhejiang,China
關(guān)鍵詞:
骨碎補(bǔ) 骨質(zhì)疏松; 絕經(jīng)后 膠原Ⅰ型 骨密度 大鼠 動(dòng)物實(shí)驗(yàn)
Keywords:
Drynaria fortunei;  Osteoporosis; postmenopausal;  Collagen typeⅠ;  Bone density;  Rats;  Animal experimentation
摘要:
目的:觀察骨碎補(bǔ)總黃酮對(duì)去卵巢大鼠骨組織Ⅰ型膠原表達(dá)及骨代謝的影響。方法:雌性SD大鼠40只,月齡10個(gè)月,隨機(jī)分為實(shí)驗(yàn)組、空白對(duì)照組、模型組和陽(yáng)性對(duì)照組,每組10只。除空白對(duì)照組外,其余各組均切除雙側(cè)卵巢制作骨質(zhì)疏松模型。造模后第8天開(kāi)始藥物干預(yù),實(shí)驗(yàn)組予以骨碎補(bǔ)總黃酮濃縮液2 mL灌胃,陽(yáng)性對(duì)照組予以倍美力混懸劑2 mL灌胃; 空白對(duì)照組和模型組予以生理鹽水2 mL灌胃; 均每日1次。藥物干預(yù)6個(gè)月后,比較各組大鼠骨密度、尿脫氧吡啶啉/尿肌酐比值、Ⅰ型膠原氨基末端肽/尿肌酐比值及血清Ⅰ型前膠原氨基端前肽含量; 并檢測(cè)各組大鼠骨組織中Ⅰ型膠原mRNA的表達(dá)。結(jié)果:藥物干預(yù)6個(gè)月后,各組大鼠間骨密度值比較,差異有統(tǒng)計(jì)學(xué)意義[(0.287±0.017)g·cm-2,(0.362±0.031)g·cm-2,(0.207±0.008)g·cm-2,(0.291±0.015)g·cm-2; F=104.317,P=0.000)]; 實(shí)驗(yàn)組低于空白對(duì)照組(P=0.000),高于模型組(P=0.000),但與陽(yáng)性對(duì)照組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.584); 空白對(duì)照組高于模型組和陽(yáng)性對(duì)照組(P=0.000,P=0.000); 模型組低于陽(yáng)性對(duì)照組(P=0.000)。各組間尿脫氧吡啶啉/尿肌酐比值(3.381±0.202,2.462±0.310,4.526±0.243,3.232±0.191)、Ⅰ型膠原氨基末端肽/尿肌酐比值(10.901±1.265,8.638±1.036,13.392±1.959,10.516±1.362)及血清Ⅰ型前膠原氨基端前肽含量[(232.081±28.452)μg·mL-1,(184.443±19.250)μg·mL-1,(283.262±33.628)μg·mL-1,(238.861±26.685)μg·mL-1]比較,差異均有統(tǒng)計(jì)學(xué)意義(F=124.841,P=0.000; F=18.277,P=0.000; F=21.648,P=0.000); 實(shí)驗(yàn)組高于空白對(duì)照組,差異均有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.000,P=0.000); 實(shí)驗(yàn)組低于模型組,差異有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.003,P=0.002); 實(shí)驗(yàn)組與陽(yáng)性對(duì)照組比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.107,P=0.521,P=0.589); 空白對(duì)照組低于模型組和陽(yáng)性對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.000,P=0.000; P=0.000,P=0.003,P=0.000); 模型組高于陽(yáng)性對(duì)照組,差異均有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.001,P=0.004)。空白對(duì)照組大鼠骨組織Ⅰ型膠原mRNA表達(dá)最強(qiáng); 模型組大鼠骨組織Ⅰ型膠原mRNA表達(dá)呈陰性或低表達(dá); 實(shí)驗(yàn)組大鼠骨組織Ⅰ型膠原mRNA表達(dá)水平與陽(yáng)性對(duì)照組接近,較模型組強(qiáng)。結(jié)論:骨碎補(bǔ)總黃酮能增強(qiáng)去卵巢大鼠骨組織Ⅰ型膠原的表達(dá),降低骨轉(zhuǎn)換率,增加骨密度; 這可能是骨碎補(bǔ)總黃酮防治骨質(zhì)疏松癥的機(jī)制之一。
Abstract:
Objective:To observe the effect of drynaria fortunei total flavonoids on collagen typeⅠ(COL-Ⅰ)expression in bone tissue and bone metabolism in the ovariectomized rats.Methods:Forty ten-month-old female SD rats were randomly divided into experimental group,blank control group,model group and positive control group,10 rats in each group.The osteoporosis rat models were built by bilateral ovariectomy in experimental group,model group and positive control group.The drug intervention were carried out from the 8th day after modeling.The rats in the experimental group were intragastric administrated with concentrated solution of drynaria fortunei total flavonoids(2 mL qd),the rats in the positive control group were intragastric administrated with Premarin suspensions(2 mL qd),while the others in the blank control group and model group were intragastric administrated with normal saline(2 mL qd).After 6 months of drug intervention,bone density,urine content ratio of deoxy pyridinoline(DPD)to creatinine(Cre),urine content ratio of N-telopeptide of typeⅠcollagen(NTx)to creatinine(Cre)and serum content of amino-terminal propeptide of type Ⅰ procollagen(PINP)were detected and compared between the 4 groups.Meanwhile,the COL-ⅠmRNA expression in bone tissues were detected.Results:After drug intervening for 6 months,there were statistical differences in the bone density between the 4 groups(0.287+/-0.017,0.362+/-0.031,0.207+/-0.008,0.291+/-0.015 g/cm(2); group was lower than that of the blank control group(P=0.000)and higher than that of model group(P=0.000),while there was no statistical difference in the bone density value between the experimental group and positive control group(P=0.584).The bone density value of the blank control group was higher than that of model group and positive control group(P=0.000,P=0.000),while the bone density value of the model group was lower than that of positive control group(P=0.000).There were statistical differences in the urine content ratio of DPD to Cre(3.381+/-0.202,2.462+/-0.310,4.526+/-0.243,3.232+/-0.191),the urine content ratio of NTx to Cre(10.901+/-1.265,8.638+/-1.036,13.392+/-1.959,10.516+/-1.362)and the serum content of PINP(232.081+/-28.452,184.443+/-19.250,283.262+/-33.628,238.861+/-26.685 μg/mL)between the 4 groups(F=124.841,P=0.000; F=18.277,P=0.000; F=21.648,P=0.000).The values of these bone metabolism indexes were higher in the experimental group compared with the blank control group(P=0.000,P=0.000,P=0.000)and were lower in the experimental group compared with the model group(P=0.000,P=0.003,P=0.002).There were no statistical differences in the values between the experimental group and the positive control group(P=0.107,P=0.521,P=0.589).The values of the blank control group were lower than those of the model group and the positive control group(P=0.000,P=0.000,P=0.000; P=0.000,P=0.003,P=0.000).The values of the model group were higher than those of the positive control group(P=0.000,P=0.001,P=0.004).Strong-positive expression of COL-ⅠmRNA in bone tissues was found in the blank control group,while negative or low expression of COL-ⅠmRNA was found in the model group.The experimental group was similar to the positive control group and surpassed the model group in the expression of COL-ⅠmRNA in bone tissues.Conclusion:The drynaria fortunei total flavonoids can enhance the COL-Ⅰexpression in bone tissues of ovariectomized rats and reduce the bone turnover rate and increase the bone density,which may be one of the mechanisms for drynaria fortunei total flavonoids in the prevention of osteoporosis.

參考文獻(xiàn)/References:

[1] 薛延.骨質(zhì)疏松癥防治指南[M].北京:人民衛(wèi)生出版社,2008:3-13.
[2] 黃淑紓,林華,朱秀芬,等.骨質(zhì)量與骨質(zhì)疏松性骨折[J].中華骨質(zhì)疏松和骨礦鹽疾病雜志,2012,5(4):285-291.
[3] 謝雁鳴,鞠大宏,趙晉寧.骨碎補(bǔ)總黃酮對(duì)去卵巢大鼠骨密度和骨組織形態(tài)計(jì)量學(xué)影響[J].中國(guó)中藥雜志,2004,29(4):343-345.
[4] 謝雁鳴,許勇鋼,趙晉寧,等.骨碎補(bǔ)總黃酮對(duì)去卵巢大鼠骨密度和細(xì)胞因子IL-6、IL-4、TNFa水平的影響[J].中國(guó)中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志,2004,10(1):34-37.
[5] 伍海昭,陳海嘯,朱敏.骨碎補(bǔ)總黃酮對(duì)去卵巢大鼠骨超微結(jié)構(gòu)及脯氨酸羥化程度的影響[J].中國(guó)骨傷,2011,24(1):48-50.
[6] 梁文娜,葉蕻芝,廖凌虹,等.Ⅰ型膠原蛋白對(duì)骨礦化機(jī)制的影響[J].中國(guó)老年學(xué)雜志,2012,32(17):3840-3842.
[7] Gelse K,Pöschl E,Aigner T.Collagens--structure,function,and biosynthesis[J].Adv Drug Deliv Rev,2003,55(12):1531-1546.
[8] 張榮華,彭勇,楊麗,等.益骨膠囊對(duì)去卵巢骨質(zhì)疏松大鼠骨組織Ⅰ型膠原mRNA表達(dá)的影響[J].時(shí)珍國(guó)醫(yī)國(guó)藥,2005,16(10):941-943.
[9] 楊國(guó)夫,宋國(guó)勝,張濤,等.牛膝提取物對(duì)去卵巢大鼠骨密度、骨轉(zhuǎn)換及Ⅰ型膠原蛋白表達(dá)的影響[J].中國(guó)骨質(zhì)疏松雜志,2011,17(2):109-112.
[10] 錢濟(jì)先,王相如,馬保安,等.吲哚昔芬對(duì)大鼠成骨細(xì)胞增殖和Ⅰ型膠原合成的影響[J].中華骨科雜志,2004,24(11):674-677.
[11] 肖恩,孟萍.骨質(zhì)疏松骨代謝生化指標(biāo)的研究進(jìn)展[J].中國(guó)骨質(zhì)疏松雜志,2008,14(3):212-216.
[12] 王桂華,趙建寧.骨質(zhì)量的影響因素及其檢測(cè)方法[J].醫(yī)學(xué)研究生學(xué)報(bào),2011,24(10):1095-1098.
[13] 王洪復(fù).骨質(zhì)疏松癥的診斷[J].國(guó)際內(nèi)分泌代謝雜志,2006,26(4):285-288.
[14] 段斌斌,萬(wàn)全增,李春雯,等.骨碎補(bǔ)對(duì)OPG/RANKL表達(dá)影響的研究進(jìn)展[J].中醫(yī)正骨,2011,23(6):40-42.

更新日期/Last Update: 1900-01-01