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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:

第26卷

期數(shù):

2014年04期

頁碼:

7-10

欄目:

基礎(chǔ)研究

出版日期:

2014-04-30

文章信息/Info

Title:

A biocompatibility study on carbon-carbon composite lumbar cage coated with hydroxyapatites

作者:

王文波¹; 董建文¹; 曹寧²; 楊振國¹; 劉文斌¹; 李木森³; 李春娥¹

1.山東中醫(yī)藥大學(xué),山東 濟(jì)南 250355; 2.中國石油大學(xué)(華東),山東 青島 266580; 3.山東大學(xué),山東 濟(jì)南 250061

Author(s):

Wang Wenbo^*; Dong Jianwen; Cao Ning; Yang Zhenguo; Liu Wenbin; Li Musen; Li Chune.^*

Shandong University of Traditional Chinese Medicine,Jinan 250355,Shangdong,China

關(guān)鍵詞:

腰椎 碳-碳復(fù)合材料 羥基磷灰石類 腰椎融合器 動(dòng)物實(shí)驗(yàn)

Keywords:

Lumbar vertebrae;  Carbon-carbon composites;  Hydroxyapatites;  Lumbar cage;  Animal experimentation

摘要:

目的:探討碳-碳復(fù)合材料羥基磷灰石涂層腰椎融合器的生物相容性和融合效果。方法:在8只成年雄性雜交波爾山羊L_4～5或L_5～6椎間隙模擬椎間融合手術(shù),在相應(yīng)椎間隙置入自制的碳-碳復(fù)合材料羥基磷灰石涂層腰椎融合器,并以克氏針后路固定。觀察記錄山羊術(shù)后生命體征及雙下肢活動(dòng)情況; 分別于術(shù)前和處死動(dòng)物前進(jìn)行腰椎X線檢查,觀察腰椎融合器的位置、融合器與上下位椎體的融合情況及上下位椎體的椎間隙; 分別于術(shù)后60 d和90 d各處死4只山羊,取融合的2個(gè)椎體,分別以肉眼、光鏡和電鏡進(jìn)行組織學(xué)觀察。結(jié)果:X線檢查顯示,術(shù)前、術(shù)后60 d和術(shù)后90 d腰椎融合器上下位椎體間距分別為(2.32±0.31)mm、(2.41±0.43)mm和(2.40±0.44)mm; 術(shù)后60 d和術(shù)后90 d,與融合器相鄰的終板位置有成骨現(xiàn)象和融合現(xiàn)象。組織學(xué)觀察顯示,術(shù)后60 d時(shí)融合器植骨區(qū)和融合器周圍有骨小梁生成; 術(shù)后90 d時(shí)骨小梁排列逐漸規(guī)則,融合器周圍有一層纖維結(jié)締組織生成,結(jié)締組織逐漸變薄,融合器中間有骨組織和纖維組織長入。結(jié)論:碳-碳復(fù)合材料羥基磷灰石涂層腰椎融合器具有較好的生物相容性和穩(wěn)定性,有利于植骨融合,可作為椎間融合的內(nèi)固定器材。

Abstract:

Objective:To explore the biocompatibility of carbon-carbon composite lumbar cage coated with hydroxyapatites(HA)and the effectiveness of fusion.Methods:The simulated intervertebral fusion surgery were performed in L₄/L₅ or L₅/L₆ intervertebral space in 8 adult male hybrid Boer goats.The self-made carbon-carbon composite lumbar cage coated with HA were placed in the intervertebral space and fixed with Kirschner-wire in posterior access.Vital signs and both lower extremities activities of the Boer goats were observed and recorded after the surgery.Radiography were performed in the lumbar vertebraes before the surgery and before the goats were sacrificed respectively.The location of lumbar cage,fusion of lumbar cage and vertebral bodies and the intervertebral space were observed.Four goats were sacrificed at 60th days and 90th days after the surgery respectively,and the two confertus vertebral bodies were fetched,and the histological observation was carried out under naked eye,optical microscope and electron microscope.Results:The X-ray examination showed that the height of intervertebral space where the lumbar cage were placed were 2.32+/-0.31,2.41+/-0.43 and 2.40+/-0.44 mm respectively before the surgery,60 days and 90 days after the surgery.Bone formation and fusion were found in the endplate adjacent to lumbar cage 60 days and 90 days after the surgery.The histological observation showed that the trabeculae of bone could be found in the bone graft area of lumbar cage and around the lumbar cage 60 days after the surgery.The trabecular bone arranged regularly at the 90 days after the surgery,a layer of fibrous connective tissue were found around the cage and it grow thin gradually,and the bone tissue and fibrous tissue grew into the cage.Conclusion:The carbon-carbon composite lumbar cage coated with HA has good biocompatibility and stability,and it is helpful to bone graft fusion,so it can be used as the internal fixation apparatus for intervertebral fusion.

備注/Memo

備注/Memo:

基金項(xiàng)目:山東省醫(yī)藥衛(wèi)生發(fā)展計(jì)劃項(xiàng)目(2007HZ093),山東省濟(jì)南市科技發(fā)展計(jì)劃項(xiàng)目(201102063) 通訊作者:董建文 E-mail:[email protected]

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更新日期/Last Update: 1900-01-01