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[1]史曉林,李春雯,張志強.弱陽離子磁珠分離技術(shù)和基質(zhì)輔助激光解吸電離飛行時間質(zhì)譜技術(shù)在原發(fā)性Ⅰ型骨質(zhì)疏松癥血清標志蛋白篩選中的應(yīng)用[J].中醫(yī)正骨,2014,26(03):5-8.
　Shi Xiaolin*,Li Chunwen,Zhang Zhiqiang..Application of magnetic beads based weak cation exchange separation technology and matrix-assisted laser desorption-ionization time of flight mass spectrometry technology in screening serum protein markers of primary type-Ⅰ osteoporosis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(03):5-8.
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弱陽離子磁珠分離技術(shù)和基質(zhì)輔助激光解吸電離飛行時間 質(zhì)譜技術(shù)在原發(fā)性Ⅰ型骨質(zhì)疏松癥血清標志蛋白篩選中 的應(yīng)用()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期數(shù):
2014年03期
頁碼:
5-8
欄目:
基礎(chǔ)研究
出版日期:
2014-03-30

文章信息/Info

Title:
Application of magnetic beads based weak cation exchange separation technology and matrix-assisted laser desorption-ionization time of flight mass spectrometry technology in screening serum protein markers of primary type-Ⅰ osteoporosis
作者:
史曉林1; 李春雯2; 張志強2
1.浙江中醫(yī)藥大學附屬第二醫(yī)院,浙江 杭州 310005;
2.浙江中醫(yī)藥大學,浙江 杭州 310053
Author(s):
Shi Xiaolin*; Li Chunwen; Zhang Zhiqiang.
*The Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou 310005,Zhejiang,China
關(guān)鍵詞:
骨質(zhì)疏松; 絕經(jīng)后 弱陽離子磁珠 光譜法; 質(zhì)量; 基質(zhì)輔助激光解吸電離 蛋白質(zhì)組學
Keywords:
Osteoporosis; Postmenopausal;  Magnetic beads based weak cation exchange;  Spectrometry; mass; matrix-assisted laser desorption-ionization;  Proteomics
摘要:
目的:探討弱陽離子磁珠分離技術(shù)和基質(zhì)輔助激光解吸電離飛行時間質(zhì)譜技術(shù)在原發(fā)性Ⅰ型骨質(zhì)疏松癥血清標志蛋白篩選中的應(yīng)用價值,尋找對原發(fā)性Ⅰ型骨質(zhì)疏松癥進行篩查和早期診斷的新方法。方法:應(yīng)用弱陽離子磁珠分離技術(shù)和基質(zhì)輔助激光解吸電離飛行時間質(zhì)譜技術(shù)對16例絕經(jīng)后骨質(zhì)疏松癥患者及9例絕經(jīng)后健康婦女的血清蛋白進行篩選、分析,將經(jīng)支持向量機模型預測的約登指數(shù)最高的差異蛋白峰作為候選標志物建立診斷模型,并對模型的判別效果進行驗證。結(jié)果:2組血清蛋白標本共初選出123個遞加質(zhì)譜蛋白質(zhì)峰。篩選出強度差異具有統(tǒng)計學意義的蛋白質(zhì)峰16組,約登指數(shù)最高的4組差異蛋白質(zhì)峰的質(zhì)荷比分別為8 909.047、8 690.658、13 745.48、15 114.52。以這4個標志物作為候選標志物建立診斷模型,模型特異性、敏感性均為100%。2組標本在支持向量機結(jié)果散點圖上可清晰區(qū)分。結(jié)論:采用弱陽離子磁珠分離技術(shù)和基質(zhì)輔助激光解吸電離飛行時間質(zhì)譜技術(shù)可篩選出原發(fā)性Ⅰ型骨質(zhì)疏松癥血清標志蛋白、建立診斷模型,為骨質(zhì)疏松癥的篩查和診斷提供了一種全新的血清學方法。
Abstract:
Objective:To evaluate the value of magnetic beads based weak cation exchange separation technology and matrix-assisted laser desorption-ionization time of flight mass spectrometry(MALDI-TOF-MS)technology in screening serum protein markers of primary type-Ⅰ osteoporosis,so as to find new methods for screening and early diagnosis of primary type-Ⅰ osteoporosis.Methods:The serum protein of 16 patients with postmenopausal osteoporosis and 9 postmenopausal healthy women were screened and analyzed using magnetic beads based weak cation exchange separation technology and MALDI-TOF-MS technology,and the differential protein peaks with the highest Youden index that predicted by support vector machine(SVM)model were chosen as candidate markers to build the diagnostic model,and then the distinguish effectiveness of the model was checked.Results:Totally 123 progressive protein peaks were originally selected from the protein peaks of 2 groups of serum protein specimens in the mass spectrogram.There was statistical differences in the intensity between 16 groups of protein peaks,and the mass charge ratio of 4 groups of differential protein peaks with the highest Youden index were 8 909.047,8 690.658,13 745.48 and 15 114.52 respectively.Then the 4 markers were used as candidate markers to build diagnostic model,and the specificity and sensitivity of the model were both 100%.The specimen in the 2 groups could be clearly distinguished by using scatterplot of SVM results.Conclusion:By using magnetic beads based weak cation exchange separation technology and MALDI-TOF-MS technology,serum protein markers of primary type-Ⅰ osteoporosis can be selected to build the diagnostic model,which provide a brand-new serological method for screening and diagnosis of osteoporosis.

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備注/Memo

備注/Memo:
基金項目:國家自然科學基金項目(81373878); 2012年浙江省自然科學基金項目(LY12H29007),2013年浙江省自然科學基金項目(LY13H290009)
通訊作者:李春雯 E-mail:[email protected]
更新日期/Last Update: 2014-03-20