84年鼠女哪年财运最旺,857comvvv色九欧美激情|85PO_87国产精品欲av国产av资源

[1]許心弦,溫宏,呂楊訓,等.信號傳導及轉(zhuǎn)錄激活因子1誘導骨細胞凋亡在激素性股骨頭壞死過程中的作用[J].中醫(yī)正骨,2014,26(02):3-7.
　Xu Xinxian*,Wen Hong,Lyu Yangxun,et al.Effect of bone cell apoptosis induced by signal transducers and activators of transcription-1 in the process of steroid-induced necrosis of femoral head[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(02):3-7.
點擊復制

信號傳導及轉(zhuǎn)錄激活因子1誘導骨細胞凋亡在激素性股骨頭 壞死過程中的作用()
分享到：

《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期數(shù):
2014年02期
頁碼:
3-7
欄目:
基礎研究
出版日期:
2014-02-20

文章信息/Info

Title:
Effect of bone cell apoptosis induced by signal transducers and activators of transcription-1 in the process of steroid-induced necrosis of femoral head
作者:
許心弦; 溫宏; 呂楊訓; 胡月正; 余華晨; 潘孝云
溫州醫(yī)科大學附屬第二醫(yī)院,浙江 溫州 325027
Author(s):
Xu Xinxian*; Wen Hong; Lyu Yangxun; Hu Yuezheng; Yu Huachen; Pan Xiaoyun.
*The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,Zhejiang,China
關鍵詞:
股骨頭壞死 糖皮質(zhì)激素類 細胞凋亡 信號傳導 轉(zhuǎn)錄激活因子類
Keywords:
Femur head necrosis;  Glucocorticoids;  Apoptosis;  Signal transduction;  Activating transcription factors
摘要:
目的:探討信號傳導及轉(zhuǎn)錄激活因子1誘導細胞凋亡在激素性股骨頭壞死過程中的作用。方法:選擇30例激素性股骨頭壞死組織標本,FicatⅠ期和Ⅱ期患者10例(A組)、FicatⅢ期患者10例(B組)、FicatⅣ期和Ⅴ期患者10例(C組),同時選擇10例接受股骨頸骨折內(nèi)固定手術的正常股骨頭組織標本(D組)。對所有標本在光鏡下觀察測定空骨陷窩率,采用TUNEL凋亡檢測法測定細胞凋亡率,分別采用免疫組化法和ELISA法對磷酸化信號傳導及轉(zhuǎn)錄激活因子1的表達進行定性和定量檢測,同時分別對細胞凋亡率與空骨陷窩率和磷酸化信號傳導及轉(zhuǎn)錄激活因子1表達量進行相關性分析。結(jié)果:4組患者股骨頭空骨陷窩率比較,差異有統(tǒng)計學意義[(29.10±6.14)%,(54.10±7.33)%,(75.25±7.80)%,(7.20±1.32)%,F=227.614,P=0.000]; A組空骨陷窩率高于D組(P=0.000),B組高于A組(P=0.000),C組高于B組(P=0.000)。4組患者股骨頭細胞凋亡率比較,差異有統(tǒng)計學意義[(5.70±3.27)%,(36.90±9.53)%,(70.30±13.63)%,(2.80±2.57)%,F=136.004,P=0.000]。A組和D組比較,差異無統(tǒng)計學意義(P=0.209); B組高于A組(P=0.000),C組高于B組(P=0.000)。4組患者股骨頭磷酸化信號傳導及轉(zhuǎn)錄激活因子1定性檢測結(jié)果比較,差異有統(tǒng)計學意義[(3.15±0.82),(5.97±1.01),(8.56±0.94),(1.00±0.56),F=150.005,P=0.000]。A組高于D組(P=0.000),B組高于A組(P=0.000),C組高于B組(P=0.000)。4組患者股骨頭磷酸化信號傳導及轉(zhuǎn)錄激活因子1定量檢測結(jié)果比較,差異有統(tǒng)計學意義[(1.86±0.43),(5.04±2.24),(11.14±2.49),(1.00±0.28),F=73.466,P=0.000]。A組高于D組(P=0.000),B組高于A組(P=0.000),C組高于B組(P=0.000)。空骨陷窩率和細胞凋亡率呈正相關(rs=0.882,P=0.000),細胞凋亡率和磷酸化信號傳導及轉(zhuǎn)錄激活因子1表達量呈正相關(rs=0.860,P=0.000)。結(jié)論:骨細胞凋亡是激素性股骨頭壞死的重要過程,并且信號傳導及轉(zhuǎn)錄激活因子1可能在其中發(fā)揮了重要作用。
Abstract:
Objective:To explore the effect of bone cell apoptosis induced by signal transducers and activators of transcription-1(STAT1)in the process of steroid-induced necrosis of femoral head(SNFH).Methods:The tissue samples of femoral head were fetched from 30 patients with SNFH.Ten patients(Group A)were categorized as Ficat phaseⅠorⅡ,10 patients(Group B)as Ficat phaseⅢ,10 patients(Group C)as Ficat phase Ⅳ or Ⅴ.Meanwhile,ten normal bone tissue samples(Group D)were obtained from internal fixation surgery for femoral neck fractures.All the samples were observed under the optical microscope and the empty bone lacuna rate were measured,then the TUNEL apoptotic detection method were used to detect the apoptosis rate.After that,qualitative and quantitative detection on the expression of phosphorylated STAT1(p-STAT1)were carried out by immunohistochemical method and ELISA method respectively.Meanwhile,correlation analysis were applied to study the relationship between empty bone lacuna rate and apoptosis rate and between empty bone lacuna rate and p-STAT1 expression.Results:There was statistical difference in the empty bone lacuna rate of femur head among the 4 groups(29.10+/-6.14%,54.10+/-7.33%,75.25+/-7.80%,7.20+/-1.32%,F=227.614,P=0.000).The empty bone lacuna rate was higher in group A compared with group D(P=0.000),was higher in group B compared with group A(P=0.000),and was higher in group C compared with group B(P=0.000).There was statistical difference in the apoptosis rate of femur head between the 4 groups(5.70+/-3.27%,36.90+/-9.53%,70.30+/-13.63%,2.80+/-2.57%,F=136.004,P=0.000).There was no statistical difference in the apoptosis rate of femur head between group A and group D(P=0.209).The apoptosis rate was higher in group B compared with group A (P=0.000)and was higher in group C compared with group B(P=0.000).There was statistical difference in the results of qualitative detection of femur head p-STAT1 between the 4 groups(3.15+/-0.82,5.97+/-1.01,8.56+/-0.94,1.00+/-0.56,F=150.005,P=0.000).Group A surpassed group D(P=0.000),group B surpassed group A(P=0.000),and group C surpassed group B(P=0.000).There was statistical difference in the results of quantitative detection of femur head p-STAT1 between the 4 groups(1.86+/-0.43,5.04+/-2.24,11.14+/-2.49,1.00+/-0.28,F=73.466,P=0.000).Group A surpassed group D(P=0.000),group B surpassed group A(P=0.000),and group C surpassed group B(P=0.000).The results of correlation analysis showed that the empty bone lacuna rate and the expression quantity of p-STAT1 were positively correlated with the apoptosis rate(rs=0.882,P=0.000; rs=0.860,P=0.000).Conclusion:STAT1 may play an important role in bone cell apoptosis,which is important for the process of SNFH.

參考文獻/References:

[1] Weinstein RS,Nicholas RW,Manolagas SC.Apoptosis of osteocytes in glucocorticoid-induced osteonecrosis of the hip[J].J Clin Endocrinol Metab,2000,85(8):2907-2912.
[2] 熊明月,王坤正,黨曉謙.早期激素性股骨頭壞死骨細胞凋亡的實驗研究[J].中國修復重建外科雜志,2007,21(3):262-265.
[3] Kostanian IA,Vonarshenko AV,Lipkin VM.STAT1:a many-sided transcription factor[J].Bioorg Khim,2010,36(1):15-28.
[4] Kothapalli R,Aya-ay JP,Bian H,et al.Ischaemic injury to femoral head induces apoptotic and oncotic cell death[J].Pathology,2007,39(2):241-246.
[5] Baofeng L,Zhi Y,Bei C,et al.Characterization of a rabbit osteoporosis model induced by ovariectomy and glucocorticoid[J].Acta Orthop,2010,81(3):396-401.
[6] James J,Steijn-Myagkaya GL.Death of osteocytes.Electron microscopy after in vitro ischaemia[J].J Bone Joint Surg Br,1986,68(4):620-624.
[7] Himpe E,Kooijman R.Insulin-like growth factor-I receptor signal transduction and the Janus Kinase/Signal Transducer and Activator of Transcription(JAK-STAT)pathway[J].Biofactors,2009,35(1):76-81.

備注/Memo

備注/Memo:
基金項目:溫州市科技局資助項目(Y20120252)
通訊作者:潘孝云 E-mail:[email protected]
更新日期/Last Update: 2014-02-20