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[1]閆東,景金珠,于愛紅,等.骺板損傷的顯微CT表現(xiàn)及病理變化研究[J].中醫(yī)正骨,2014,26(01):6-10.
　Yan Dong*,Jing Jinzhu,Yu Aihong,et al.A dynamic study on micro computed tomography manifestation and pathological change of epiphyseal plate injury in rat model[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2014,26(01):6-10.
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骺板損傷的顯微CT表現(xiàn)及病理變化研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第26卷
期數(shù):
2014年01期
頁碼:
6-10
欄目:
基礎(chǔ)研究
出版日期:
2014-01-28

文章信息/Info

Title:
A dynamic study on micro computed tomography manifestation and pathological change of epiphyseal plate injury in rat model
作者:
閆東; 景金珠; 于愛紅; 白榮杰; 程曉光
北京積水潭醫(yī)院,北京 100035
Author(s):
Yan Dong*; Jing Jinzhu; Yu Aihong; Bai Rongjie; Cheng Xiaoguang.
*Beijing Jishuitan Hospital,Beijing 100035,China
關(guān)鍵詞:
生長面 骨骺 顯微CT 病理過程 動物實(shí)驗(yàn)
Keywords:
Growth plate;  Epiphyses;  Micro computed tomography;  Pathologic processes;  Animal experimentation
摘要:
目的:探討實(shí)驗(yàn)性骺板損傷修復(fù)過程中的顯微CT表現(xiàn)及病理變化。方法:取4～5周齡Wistar大鼠50只,選擇右側(cè)脛骨制作骺板損傷動物模型,左側(cè)作為對照。分別于造模后1、3、7、11和21 d各選取10只大鼠處死,取雙側(cè)脛骨行顯微CT掃描,觀察損傷骺板的影像特征,并通過組織學(xué)切片HE染色,觀察骺板內(nèi)組織細(xì)胞的動態(tài)改變。結(jié)果:①對照側(cè)骺板顯微CT及組織形態(tài)表現(xiàn)。造模后1 d,顯微CT圖像顯示骨骺與干骺端高密度帶間波浪形帶狀低密度區(qū),邊緣清晰; 造模后7 d,骺板輕度變窄; 11 d后逐漸明顯變窄,中間部位較邊緣顯著; 21 d時骺板區(qū)密度增高,明顯變窄。造模后1 d,骺板HE染色切片示骺板4層結(jié)構(gòu)清晰可見,軟骨細(xì)胞呈柱狀規(guī)律排列; 7 d后幼稚軟骨細(xì)胞逐漸減少,增殖層細(xì)胞柱逐漸稀疏,肥大層寬度減小,細(xì)胞膨大、退化,骺板逐漸變窄,其中以造模后21 d表現(xiàn)最明顯。②觀察側(cè)骺板顯微CT及組織形態(tài)表現(xiàn)。造模后1 d,顯微CT可見干骺端骨折; 造模后3～7 d,骺板寬度先增加,然后趨于正常; 11 d時骺板寬度減小伴新生骨質(zhì)形成,干骺端下見迂曲透亮影; 21 d時骺板寬度明顯變窄,骨橋形成。HE染色顯示損傷早期骺板細(xì)胞正常結(jié)構(gòu)消失,肥大層結(jié)構(gòu)紊亂,組織出血、水腫; 造模后7 d時靜止層細(xì)胞聚集,并大量出現(xiàn)在損傷區(qū),同時軟骨細(xì)胞增殖活躍; 11 d時損傷區(qū)出現(xiàn)大量靜止層細(xì)胞,軟骨細(xì)胞增殖活躍,成簇的軟骨細(xì)胞團(tuán)伸入干骺端; 21 d時損傷區(qū)見成熟小梁骨橫貫干骺端至骨骺。結(jié)論:顯微CT空間分辨率高,可動態(tài)反映骺板損傷的修復(fù)過程,是研究實(shí)驗(yàn)性小動物骺板損傷理想的影像學(xué)檢查方法。
Abstract:
Objective:To observe the micro computed tomography(CT)manifestation and pathological change of experimental epiphyseal plate injury during repairs.Methods:The right tibiaes were fetched out from fifty Wistar rats(aged 4-5 weeks)to build epiphyseal plate injury animal models,while the left tibiaes were treated as controls.At 1,3,7,11 and 21 days after modeling,10 rats were executed,and their bilateral tibias were fetched out and received micro CT scanning.The imaging features of epiphyseal plate injury were observed.Then the tibias were made into sections and the dynamic changes of cells within epiphyseal plate were observed after HE staining.Results:On the 1st day after modeling,micro CT images of left tibias showed the low density girdle-shaped area with clear margin between high density areas of osteoepiphysis and metaphysis,and the epiphyseal plate became narrow slightly 7 days after modeling.On the 11st day after modeling,the epiphyseal plate became narrow obviously,and more obvious changes were found in the centre area compared to the margin area.The epiphyseal plate became narrow obviously and its density increased at 21 days after modeling.On the 1st day after modeling,HE staining clearly showed 4 layer structure of epiphyseal plate and cartilage cells which lined up in columnar style.Seven days after modeling,the immature cartilage cells increased gradually,the cell columns in proliferation layer were sparse,the width of hypertrophic layer decreased,the cells swelled and degenerated,and the epiphyseal plate became narrow gradually.The most obvious change were found at 21 days after modeling.Metaphysis fractures were found in right tibias by micro CT scanning at 1 day after modeling.Between 3 and 7 days after modeling,the width of epiphyseal plate increased at first and then tended to be normal.At 11 days after modeling,the width of epiphyseal plate decreased with new bone formation,and the curved lucent shadow were found in the metaphysis.At 21 days after modeling,the width of epiphyseal plate decreased obviously and the bone bridge could be found.The result of HE staining in sections from the right tibias showed that the normal structures of epiphyseal plate cell were absent and the structures of hypertrophic layer were indiscriminate and the hemorrhage and edema of tissues occured in earlier period after injury.On the 7th day after modeling,the cells gathered in quiescent layer and appeared in the injury zone in large number,at the same time,the cartilage cells proliferated actively.At 11 days after modeling,a large amount of quiescent layer cells appeared in injury zone,and the cartilage cells proliferated actively,and clusters of cartilage cells stretched into metaphysis.At 21 days after modeling,mature trabecular bones traversed metaphysis and osteoepiphysis in the injury zone.Conclusion:With high spatial resolution micro CT can dynamically reflect the reparative process of epiphyseal plate injure,so it is an ideal radiological examination for study of epiphyseal plate injury in small experimental animals.

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備注/Memo

備注/Memo:
基金項(xiàng)目:國家自然科學(xué)基金(81071131),北京市衛(wèi)生系統(tǒng)高層次衛(wèi)生技術(shù)人才培養(yǎng)項(xiàng)目(2009-2-03)
通訊作者:程曉光 E-mail:[email protected]
更新日期/Last Update: 2014-01-20