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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:

第25卷

期數(shù):

2013年12期

頁(yè)碼:

11-14

欄目:

骨質(zhì)疏松癥

出版日期:

2013-12-30

文章信息/Info

Title:

Impact of HUQIAN PILL on bone density and transforming growth factor beta1 expression in the ovariectomized osteoporosis rat model

作者:

謝義松¹; 張紫銘²; 吳官保³; 朱建華²; 尤冬春²

1.湖南中醫(yī)藥大學(xué)第二附屬醫(yī)院,湖南 長(zhǎng)沙 410005;
2.湖南中醫(yī)藥大學(xué), 湖南 長(zhǎng)沙 410007;
3.湖南省中醫(yī)藥研究院附屬醫(yī)院,湖南 長(zhǎng)沙 410006

Author(s):

Xie Yisong^*; Zhang Ziming; Wu Guanbao; Zhu Jianhua; You Dongchun.^*

The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,Hunan,China

關(guān)鍵詞:

骨質(zhì)疏松 骨密度 轉(zhuǎn)化生長(zhǎng)因子β1 動(dòng)物實(shí)驗(yàn) 大鼠 虎潛丸

Keywords:

Osteoporosis;  Bone density;  Transforming growth factor beta1;  Animal experimentation;  Rats;  HUQIAN PILL

摘要:

目的:探討虎潛丸治療骨質(zhì)疏松癥的作用機(jī)制。方法:雌性SD大鼠40只,體質(zhì)量180～220 g,中位數(shù)200 g,隨機(jī)分為虎潛丸組、雌激素組、模型組、假手術(shù)組,每組10只。虎潛丸組、雌激素組、模型組切除雙側(cè)卵巢造模。7 d后,虎潛丸組予以虎潛丸水煎液灌胃,雌激素組予以尼爾雌醇混懸液灌胃,模型組、假手術(shù)組予以蒸餾水灌胃; 每100 g體質(zhì)量0.875 mL,每日灌胃1次,連續(xù)干預(yù)12周。比較各組大鼠椎骨骨密度及椎骨、腎組織中轉(zhuǎn)化生長(zhǎng)因子β1的表達(dá)。結(jié)果:虎潛丸組和雌激素組各有1只大鼠死亡,共納入研究38只。干預(yù)12周后,各組大鼠椎骨骨密度值比較,差異有統(tǒng)計(jì)學(xué)意義[(0.210±0.028)g·cm^-2,(0.205±0.030)g·cm^-2,(0.171±0.036)g·cm^-2,(0.205±0.034)g·cm^-2; F=7.561,P=0.001]; 虎潛丸組、雌激素組、假手術(shù)組均高于模型組(P=0.002,P=0.002,P=0.013); 虎潛丸組與雌激素組、假手術(shù)組相比,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.459,P=0.224); 雌激素組與假手術(shù)組相比,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.231)。各組大鼠椎骨轉(zhuǎn)化生長(zhǎng)因子β1灰度值比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(169.060±5.545,167.420±6.238,162.400±3.882,168.130±7.899; F=2.176,P=0.109)。各組大鼠腎組織轉(zhuǎn)化生長(zhǎng)因子β1光密度值比較,差異有統(tǒng)計(jì)學(xué)意義(172.600±5.019,158.200±8.038,155.130±4.542,157.570±5.524; F=15.568,P=0.000); 虎潛丸組高于雌激素組、模型組和假手術(shù)組(P=0.000; P=0.000; P=0.000); 雌激素組與模型組、假手術(shù)組比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.478,P=0.490); 假手術(shù)組高于模型組(P=0.038)。結(jié)論:虎潛丸可增加大鼠腰椎椎骨骨密度,上調(diào)腎組織中轉(zhuǎn)化生長(zhǎng)因子β1的表達(dá),可能是其治療骨質(zhì)疏松癥的機(jī)制之一。

Abstract:

Objective:To explore the mechanisms of action of HUQIAN PILL in the treatment of osteoporosis.Methods:Forty female SD rats,ranged in weight from 180 to 220 g(Median=200 g),were randomly divided into HUQIAN PILL group,estrogen group,model group and sham-operation group,10 rats in each group.The rats models of osteoporosis were created by ovariectomy in HUQIAN PILL group,estrogen group and model group.Seven days after operation,rats were intragastric administrated with HUQIAN PILL decoction,nilestriol suspension and distilled water(0.875 mL/100 g)respectively,one time daily for 12 consecutive weeks.The bone density of vertebrae and transforming growth factor beta1(TGFβ1)expression in vertebra and nephridial tissue were detected and compared among the 4 groups.Results:One rat died in HUQIAN PILL group and one rat died in estrogen group,so 38 rats were included in the study.After 12 weeks of drug intervention,there were statistical differences in the bone density values of rat vertebra among the 4 groups(0.210+/-0.028,0.205+/-0.030,0.171+/-0.036,0.205+/-0.034 g/cm(2),F=7.561,P=0.001).The bone density values of HUQIAN PILL group,estrogen group and sham-operation group were all higher than that of model group(P=0.002,P=0.002,P=0.013).The HUQIAN PILL group was similar to estrogen group and sham-operation group in the bone density values(P=0.459,P=0.224),and there were no statistical differences between estrogen group and sham-operation group(P=0.231).There were no statistical differences in the gray values of TGF-β1 in vertebra among the 4 groups(169.060+/-5.545,167.420+/-6.238,162.400+/-3.882,168.130+/-7.899,F=2.176,P=0.109).There were statistical differences in the optical density(OD)values of TGF-β1 in nephridial tissue among the 4 groups(172.600+/-5.019,158.200+/-8.038,155.130+/-4.542,157.570+/-5.524,F=15.568,P=0.000).The OD value of TGF-β1 of HUQIAN PILL group was higher than that of estrogen group,model group and sham-operation group respectively(P=0.000,P=0.000,P=0.000).Estrogen group was similar to model group and sham-operation group(P=0.478,P=0.490)in the OD value of TGF-β1,and the sham-operation group surpassed the model group(P=0.038).Conclusion:The bone density of lumbar vertebra can be increased and the expression of TGF-β1 in nephridial tissue can be up-regulated in rat model by using HUQIAN PILL,and it may be one of the mechanisms of action for HUQIAN PILL in the treatment of osteoporosis.

備注/Memo

備注/Memo:

2013-08-23收稿 2013-10-13修回
基金項(xiàng)目:湖南省科技廳科研基金項(xiàng)目(2011SK3106)

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更新日期/Last Update: 2013-12-30