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[1]李新建,于蘭英,鄧偉,等.淫羊藿防治激素性股骨頭壞死的作用機制研究[J].中醫(yī)正骨,2013,25(11):3-7.
　Li Xinjian*,Yu Lanying,Deng Wei,et al.Mechanism of action of epimedium brevicornum in the prevention of steroid-induced osteonecrosis of femoral head[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2013,25(11):3-7.
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淫羊藿防治激素性股骨頭壞死的作用機制研究()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第25卷
期數(shù):
2013年11期
頁碼:
3-7
欄目:
基礎研究
出版日期:
2013-11-30

文章信息/Info

Title:
Mechanism of action of epimedium brevicornum in the prevention of steroid-induced osteonecrosis of femoral head
作者:
李新建1; 于蘭英2; 鄧偉1; 王沛2; 田小林2; 馮柯2
1.長春中醫(yī)藥大學附屬醫(yī)院,吉林 長春 130021;
2.長春中醫(yī)藥大學,吉林 長春 130117
Author(s):
Li Xinjian*; Yu Lanying; Deng Wei; Wang Pei; Tian Xiaolin; Feng Ke.*
The Affiliated Hospital to Changchun University of Chinese Medicine,Changchun 130021,Jilin,China
關鍵詞:
股骨頭壞死 骨密度 膽固醇 淫羊藿 動物實驗
Keywords:
Epimedium brevicornum;  Femur head necrosis;  Bone density;  Cholesterol;  Animal experimentation
摘要:
目的:探討淫羊藿防治激素性股骨頭壞死的作用機制。方法:將50只家兔隨機分為5組,每組10只。B、C、D、E組家兔采用臀肌注射醋酸潑尼松龍的方法進行激素性股骨頭壞死造模,A組家兔臀肌注射生理鹽水。造模結束后,C、D、E組根據(jù)體質量分別按1.2 g·kg-1、0.6 g·kg-1和0.3 g·kg-1以淫羊藿提取液灌胃,每天1次,連續(xù)灌胃10周; A組和B組以10 mL生理鹽水灌胃。分別于藥物干預開始前(0周)及藥物干預4周、6周、8周、10周后測定各組家兔血液中膽固醇和甘油三酯含量,同時在藥物干預開始前(0周)及藥物干預6周、10周后測定家兔股骨近端骨密度,并于藥物干預10周后處死家兔,取雙側股骨頭切片進行HE染色,觀察骨小梁和骨陷窩的變化。結果:①血脂。藥物干預開始后不同時間,家兔血膽固醇含量的差異總體上無統(tǒng)計學意義,即不存在時間效應(F=1.136,P=0.314); 各組家兔血膽固醇含量的組間差異總體上有統(tǒng)計學意義,即存在分組效應(F=12.874,P=0.000),除0周和4周外,其余各時點B組均高于A、C、D、E組; 時間因素和分組因素之間存在交互效應(F=4.910,P=0.001)。藥物干預開始后不同時間,家兔血甘油三酯含量的差異有統(tǒng)計學意義,即存在時間效應(F=144.191,P=0.000); 各組家兔血甘油三酯含量的組間差異總體上有統(tǒng)計學意義,即存在分組效應(F=12.358,P=0.000),B組和E組各時點血甘油三酯含量比較,差異均無統(tǒng)計學意義; 除0周外,其余各時點B組血甘油三酯含量均高于A、C、D組; 時間因素和分組因素之間存在交互效應(F=8.798,P=0.000)。②骨密度。藥物干預開始后不同時間,家兔股骨近端骨密度的差異有統(tǒng)計學意義,即存在時間效應(F=289.909,P=0.000); 除A組外,其余4組骨密度均呈下降趨勢,B組下降趨勢最明顯; 各組家兔骨密度的組間差異總體上有統(tǒng)計學意義,即存在分組效應(F=72.539,P=0.000),除0周外,其余各時點B組骨密度均低于A、C、D、E組; 時間因素和分組因素之間存在交互效應(F=40.182,P=0.000)。③病理學檢查結果。藥物干預10周后,與其余4組相比,B組骨小梁廣泛變細、斷裂; 5組家兔骨小梁面積分數(shù)和空骨陷窩率比較,組間差異均有統(tǒng)計學意義(F=62.032,P=0.000; F=20.515,P=0.000); B組骨小梁面積分數(shù)小于A、C、D、E組(P=0.000,P=0.000,P=0.000,P=0.000),空骨陷窩率大于A、C、D、E組(P=0.000,P=0.000,P=0.000,P=0.000)。結論:淫羊藿能夠降低股骨頭壞死家兔血液中膽固醇和甘油三酯的含量、延緩骨密度降低、降低空骨陷窩率、提高骨小梁面積分數(shù),從而具有防治激素性股骨頭壞死的作用。
Abstract:
Objective:To explore the mechanism of action of epimedium in the prevention of steroid-induced osteonecrosis of femoral head(SNFH).Methods:Fifty rabbits were randomly divided into 5 groups,10 cases in each group.The rabbit SNFH models were created in group B,C,D and E by intragluteus injection of prednisolone acetate,while rabbits in group A were administrated with intragluteus injection of normal saline.After that,rabbits in group C,D and E were intragastric administrated with aqueous extract of epimedium(1.2,0.6 and 0.3 g/kg,respectively),while the others in group A and group B were intragastric administrated with normal saline(10 ml),once per day for 10 consecutive weeks.Serum levels of cholesterol and triglyceride in rabbits were determined before drug intervention(0 week)and 4,6,8 and 10 weeks after the beginning of the drug intervention.The proximal femoral bone mineral density(BMD)were measured before drug intervention(0 week)and 6 and 10 weeks after the beginning of the drug intervention.At the end of the drug intervention,the rabbits were sacrificed and the bilateral femoral heads were sectioned and the change in bone trabecula and bone lacuna were observed though HE staining.Results:In general,there was no statistical difference in serum level of cholesterol(CHOL)among time points(F=1.136,P=0.314),in other words,there was no time effect.There was statistical difference in serum CHOL level among groups in general(F=12.874,P=0.000),in other words,there was group effect.The serum CHOL level of group B was higher than that of group A,C,D and Eexcept 0 and 4 weeks after drug intervention.There was interaction between time factor and grouping factor(F=4.910,P=0.001).There was statistical difference in serum level of triglyceride(TG)among time points(F=144.191,P=0.000),in other words,there was time effect.In general,there was statistical difference in serum TG level among groups(F=12.358,P=0.000),in other words,there was group effect.There was no statistical difference in serum TG level taken at different time points between group B and group E; the group B surpassed group A,C and D in serum TG level of at each time point except 0 week.There was interaction between time factor and grouping factor(F=8.798,P=0.000).There was statistical difference in proximal femoral BMD among time points(F=289.909,P=0.000).There was a trend for decreased BMD in group B,C,D and E after drug intervention,and there was more significant decreased BMD in group B compared to other groups.There was statistical difference in BMD among different groups in general(F=72.539,P=0.000),in other words,there was group effect.The BMD of Group B was lower than that of group A,C,D and E at each time point except 0 week.There was interaction between time factor and grouping factor(F=40.182,P=0.000).The bone trabecula became slim and broken extensively in group B compared with other groups 10 weeks after the beginning of drug intervention.There were statistical differences in empty bone lacuna rate and trabecular bone area fraction among different groups(F=62.032,P=0.000; F=20.515,P=0.000),and the trabecular bone area fraction of group B was less than that of group A,C,D and E(P=0.000,P=0.000,P=0.000,P=0.000),while the empty bone lacuna rate of group B was larger than that of group A,C,D and E(P=0.000,P=0.000,P=0.000,P=0.000).Conclusion:The epimedium brevicornum can decrease serum CHOL and TG contents,delay the decrease in BMD,decrease the empty bone lacuna rate and increase the trabecular bone area fraction in the rabbits with SNFH,so it can be used in the prevention and treatment of SNFH.

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備注/Memo

備注/Memo:
2012-11-01收稿 2013-03-04修回
通訊作者:王沛 E-mail:[email protected]
更新日期/Last Update: 2013-11-30