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[1]姜苗苗,譚勇海,李佳林,等.局部注射血小板源性生長因子對大鼠跟腱末端病組織結(jié)構(gòu)的影響[J].中醫(yī)正骨,2013,25(02):8-12.
　JIANG Miao-miao*,TAN Yong-hai,LI Jia-lin,et al.Research on the influence of local injection with platelet-derived growth factor on tissue structure of rat Achilles tendon's enthesiopathy[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2013,25(02):8-12.
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局部注射血小板源性生長因子對大鼠跟腱末端病組織結(jié)構(gòu)的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第25卷
期數(shù):
2013年02期
頁碼:
8-12
欄目:
基礎(chǔ)研究
出版日期:
2013-02-20

文章信息/Info

Title:
Research on the influence of local injection with platelet-derived growth factor on tissue structure of rat Achilles tendon's enthesiopathy
作者:
姜苗苗;  譚勇海;  李佳林; 于海勇
山東省文登整骨醫(yī)院,山東 文登 264400
Author(s):
JIANG Miao-miao*; TAN Yong-hai; LI Jia-lin; YU Hai-yong.
*Wendeng Orthopedic Hospital,Wendeng 264400,Shandong,China
關(guān)鍵詞:
生長因子 血小板源性 末端病 局部注射
Keywords:
Growth factor;  Platelet-derived;  Tendinosis local;  Injection
摘要:
目的:觀察血小板源性生長因子對末端病末端組織結(jié)構(gòu)的影響,為血小板源性生長因子治療末端病提供實(shí)驗(yàn)基礎(chǔ)和理論依據(jù)。方法:對篩選后的大鼠隨機(jī)分成3組(Ⅰ、Ⅱ、Ⅲ)組,其中Ⅰ組為血小板源性生長因子注射組,Ⅱ組為生理鹽水注射組,Ⅲ組為對照組。將Ⅰ、Ⅱ組大鼠放入電刺激跳躍造模裝置里,通交流電4 次·min-1,通電電壓由小逐步加大,直到大鼠出現(xiàn)有效跳躍為止,共持續(xù)20 min,休息10 min,再持續(xù)20 min。每天訓(xùn)練1 次,每周6 次,周日休息,持續(xù)4周。實(shí)驗(yàn)過程中常規(guī)純鼠料喂養(yǎng)。每周分別對血小板源性生長因子組和生理鹽水組大鼠跟腱部位注射0.2 mL血小板源性生長因子和生理鹽水,其注射時(shí)間均為大鼠休息日,對照組不做任何處理,造模4周后處死大鼠,進(jìn)行取材,組織切片制作,HE染色。顯微鏡下觀察每個(gè)樣本跟腱末端、纖維軟骨區(qū)、跟骨區(qū)、腱骨界面、腱圍共5個(gè)區(qū)域病理變化。結(jié)果:血小板源性生長因子注射組大鼠跟腱末端區(qū)發(fā)生了輕微的病理性變化。生理鹽水注射組大鼠跟腱末端區(qū)發(fā)生了明顯的病理性變化。對照組大鼠跟腱末端區(qū)未發(fā)生明顯的病理性變化。結(jié)論:注射血小板源性生長因子可以不同程度的有效地預(yù)防大鼠跟腱末端病的發(fā)生。
Abstract:
Objective:To observe the effects of platelet-derived growth factor on structure of tissue of terminal end disease to provide experimental and theoretical basis for its application in this disease.Methods:Screened rats were randomly divided into three groups,platelet derived growth factor injection group(Group Ⅰ),saline injection group(Group Ⅱ),control group(groupⅢ).Put Group Ⅰand Group Ⅱ into electrical stimulation jumping molding device,alternating current is 4 min-1,power voltage was gradually increased until the rats appeared effective jump with duration of 20 min followed with 10 min rest and 20 min alternating current again.Training was proceed once every day and 6 times per week with Sunday rest for 4 weeks.The experimental processed in conventional pure rat feed.Platelet derived growth factor group and saline group were applied with 0.2 ml each weekend on rat Achilles tendon while the control group without any treatment,modeling rats were sacrificed after 4 weeks,material,tissue section,HE staining were carried out.Microscopic observe each sample of Achilles tendon terminal,fibrocartilage zone,calcaneal tendon area,articular surface of bone,tendon in a total of 5 regional pathological changes.Results:Platelet derived growth factor injection group rat Achilles tendon terminal region has undergone minor pathological changes.Saline injection group rat Achilles tendon terminal region had obvious pathological changes.Control rats Achilles tendon terminal area without obvious pathological changes.Conclusion:Injection of platelet derived growth factor may vary in the degree of effective prevention of rat Achilles tendon terminal disease.

參考文獻(xiàn)/References:

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更新日期/Last Update: 2013-02-20