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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:

第25卷

期數(shù):

2013年01期

頁(yè)碼:

19-22

欄目:

基礎(chǔ)研究

出版日期:

2013-01-20

文章信息/Info

Title:

Effect of AOGU CAPSULE on the detection indicators related to the osteoporosis of ovariectomized rats

作者:

李學(xué)朋

山東中醫(yī)藥大學(xué),山東 濟(jì)南 250014

Author(s):

LI Xue-peng^*

^*Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong,China

關(guān)鍵詞:

骨質(zhì)疏松 卵巢切除術(shù) 骨密度 傲骨膠囊 動(dòng)物實(shí)驗(yàn)

Keywords:

Osteoporosis;  Ovariectomy;  Bone density;  AOGU CAPSULE;  Animal experimentation

摘要:

目的:觀察傲骨膠囊對(duì)去卵巢大鼠骨質(zhì)疏松相關(guān)檢測(cè)指標(biāo)的影響。方法:將50只健康10周齡雌性SD大鼠分為5組,A組9只,B組10只,C組10只,D組11只,E組10只。A組大鼠切除卵巢周?chē)糠种?其余4組切除卵巢制成骨質(zhì)疏松模型。從造模后第11天開(kāi)始,C組、D組、E組大鼠分別按0.337 5 g·kg^-1、0.675 g·kg^-1、2.025 g·kg^-1以傲骨膠囊灌胃,A組和B組按10 mL·kg^-1以去離子水灌胃,每天1次,共13周,第13周末處死大鼠。從藥物干預(yù)開(kāi)始至處死前,每周測(cè)定1次各組大鼠的體質(zhì)量。取各組大鼠左側(cè)股骨,測(cè)定其股骨中點(diǎn)、遠(yuǎn)端和近端骨密度。取各組大鼠右側(cè)股骨,測(cè)定其骨鈣含量和骨恒重。結(jié)果:①體質(zhì)量。藥物干預(yù)前5組大鼠體質(zhì)量比較,差異有統(tǒng)計(jì)學(xué)意義(F=7.230,P=0.000)。組間兩兩比較:A組大鼠體質(zhì)量[(241.40±19.10)g]小于B組[(281.90±21.00)g]、C組[(280.70±22.60)g]、D組[(282.20±20.90)g]和E組[(281.70±23.40)g],差異均有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.000,P=0.000,P=0.000)。藥物干預(yù)13周后5組大鼠體質(zhì)量均有增加(t=4.180,P=0.000; t=15.390,P=0.000; t=12.140,P=0.000; t=15.590,P=0.000; t=11.630,P=0.000),對(duì)增加值進(jìn)行組間比較,差異有統(tǒng)計(jì)學(xué)意義(F=7.364,P=0.000)。進(jìn)一步組間兩兩比較:A組增加值[(100.20±39.25)g]小于B組[(178.10±36.78)g]和D組[(143.10±29.18)g],差異均有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.008); B組增加值大于C組[(116.20±30.43)g]、D組和E組[(130.30±35.63)g],差異均有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.028,P=0.003)。其余各組間兩兩比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05)。②骨密度。5組大鼠股骨遠(yuǎn)端、股骨中心點(diǎn)及股骨近端骨密度組間比較,差異均有統(tǒng)計(jì)學(xué)意義(F=15.549,P=0.000; F=4.688,P=0.005; F=4.343,P=0.005)。B組股骨遠(yuǎn)端、股骨中心點(diǎn)及股骨近端骨密度[(0.21±0.02)g·cm^-2,(0.13±0.02)g·cm^-2,(0.18±0.03)g·cm^-2]均低于A組[(0.27±0.02)g·cm^-2,(0.16±0.01)g·cm^-2,(0.22±0.02)g·cm^-2]、D組 [(0.26±0.02)g·cm^-2,(0.16±0.03)g·cm^-2,(0.20±0.02)g·cm^-2]、E組[(0.26±0.03)g·cm^-2,(0.18±0.01)g·cm^-2,(0.21±0.01)g·cm^-2],差異均有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.001,P=0.000; P=0.000,P=0.001,P=0.039; P=0.000,P=0.000,P=0.003); C組股骨遠(yuǎn)端和股骨近端骨密度[(0.22±0.01)g·cm^-2,(0.18±0.02)g·cm^-2]低于D組,差異均有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.039); C組股骨遠(yuǎn)端、股骨中心點(diǎn)[(0.15±0.02)g·cm^-2]及股骨近端骨密度均低于E組(P=0.000,P=0.001,P=0.003); D組股骨中心點(diǎn)骨密度低于E組(P=0.027); 其余各組間比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05)。③骨鈣含量。5組大鼠骨鈣含量比較,差異有統(tǒng)計(jì)學(xué)意義(F=5.929,P=0.001)。A組骨鈣含量[(254.12±19.11)mg·g^-1]大于B組[(203.23±30.58)mg·g^-1]和C組[(226.35±26.71)mg·g^-1],差異均有統(tǒng)計(jì)學(xué)意義(P=0.000,P=0.027); B組骨鈣含量小于D組[(245.80±16.43)mg·g^-1]和E組[(239.40±37.30)mg·g^-1],差異均有統(tǒng)計(jì)學(xué)意義(P=0.001,P=0.005); 其余各組間比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05)。④骨恒重。5組大鼠骨恒重比較,差異有統(tǒng)計(jì)學(xué)意義(F=5.147,P=0.001)。E組大鼠骨恒重[(664.51±44.75)mg]大于A組[(603.77±70.05)mg]、B組[(603.38±36.57)mg]和C組[(611.93±53.03)mg],差異均有統(tǒng)計(jì)學(xué)意義(P=0.013,P=0.012,P=0.030); 其余各組間比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05)。結(jié)論:中、高劑量的傲骨膠囊能促進(jìn)去卵巢大鼠對(duì)鈣的吸收和利用,增加骨密度,具有明顯的抗骨質(zhì)疏松作用。同時(shí)傲骨膠囊還有抑制由雌激素水平降低所引起的體質(zhì)量增加的作用。

Abstract:

Objective:To observe the effect of AOGU CAPSULE on the detection indicators related to the osteoporosis of ovariectomized rats.Methods:Fifty healthy female SD rats of 10 weeks old were divided into 5 groups,9 cases in group A,10 cases in group B,10 cases in group C,11 cases in group D and 10 cases in group E.Rats in group A were administrated with fat removal around the ovary,while the others in the rest 4 groups were administrated with ovariectomy to build models of osteoporosis.Ten days after molding,the rats in group C,D and E were intragastric administrated with AOGU CAPSULE(0.3375 g/kg,0.675 g/kg and 2.025 g/kg,respectively),while the others in group A and group B were intragastric administrated with deionized water(10 mL/kg),once per day for consecutive 13 weeks.All the rats were executed at the end of the 13th week.During the period from the beginning of medicine intervention to execution,the body mass of rats of each group were measured once a week.The bone mineral density(BMD)were measured on the midpoint,distal end and proximal end of the left femur of the rats,and the bone calcium content(BCC)and bone constant weight(BCW)were measured on the right femur of the rats.Results:①Body mass:there was statistical difference in body mass among the 5 groups before medicine intervention(F=7.230,P=0.000).As the results of further pairwise comparison showed,body mass of rats in group A((241.40±19.10)g)were lower than that of group B((281.90±21.00)g),C((280.70±22.60)g),D((282.20±20.90)g)and E((281.70±23.40)g)respectively,and there were statistical differences between them(P=0.000,P=0.000,P=0.000,P=0.000).Body mass of rats in the 5 groups were all increased 13 weeks after medicine intervention(t=4.180,P=0.000; t=15.390,P=0.000; t=12.140,P=0.000; t=15.590,P=0.000; t=11.630,P=0.000),and there was statistical difference in the increase value among the 5 groups(F=7.364,P=0.000).As the results of further pairwise comparison showed,the increase value of group A((100.20±39.25)g)was lower than that of group B((178.10±36.78)g)and group D((143.10±29.18)g)respectively,and there were statistical differences between them(P=0.000,P=0.008); the increase value of group B was larger than that of group C((116.20±30.43)g),D and E((130.30±35.63)g)respectively,and there were statistical differences between them(P=0.000,P=0.028,P=0.003).There was no statistical difference between any couple of the rest groups(P>0.05).②BMD:there were statistical differences in BMD on distal femur,femoral midpoint and proximal femur among the 5 groups(F=15.549,P=0.000; F=4.688,P=0.005; F=4.343,P=0.005).BMD on distal femur,femoral midpoint and proximal femur of rats in group B((0.21±0.02)g/cm(2),(0.13±0.02)g/cm(2),(0.18±0.03)g/cm(2))were all lower than those of group A((0.27±0.02)g/cm(2),(0.16±0.01)g/cm(2),(0.22±0.02)g/cm(2)),D((0.26±0.02)g/cm(2),(0.16±0.03)g/cm(2),(0.20±0.02)g/cm(2))and E((0.26±0.03)g/cm(2),(0.18±0.01)g/cm(2),(0.21±0.01)g/cm(2))respectively,and there were statistical differences between them(P=0.000,P=0.001,P=0.000; P=0.000,P=0.001,P=0.039; P=0.000,P=0.000,P=0.003); BMD on distal and proximal femurs of rats in group C((0.22±0.01)g/cm(2),(0.18±0.02)g/cm(2))were all lower than those of group D,and there were statistical differences between them(P=0.000,P=0.039); BMD on distal femur,femoral midpoint((0.15±0.02)g/cm(2))and proximal femur of rats in group D were all lower than those of group E respectively(P=0.000,P=0.001,P=0.003); BMD on femoral midpoint of group D was lower than that of group E(P=0.027); while there were no statistical differences between any couple of the rest groups(P>0.05).③BCC:there was statistical difference in BCC among the 5 groups(F=5.929,P=0.001).BCC of group A((254.12±19.11)mg/g)was larger than that of group B((203.23±30.58)mg/g)and group C((226.35±26.71)mg/g)respectively,and there were statistical differences between them(P=0.000,P=0.027); BCC of group B was lower than that of group D((245.80±16.43)mg/g)and group E((239.40±37.30)mg/g)respectively,and there were statistical differences between them(P=0.001,P=0.005); while there were no statistical differences between any couple of the rest groups(P>0.05).④BCW:there was statistical difference in BCW among the 5 groups(F=5.147,P=0.001).BCW of group E((664.51±44.75)mg)was larger than that of group A((603.77±70.05)mg),B((603.38±36.57)mg)and C((611.93±53.03)mg)respectively,and there were statistical differences between them(P=0.013,P=0.012,P=0.030); while there were no statistical differences between any couple of the rest groups(P>0.05).Conclusion:AOGU CAPSULE of medium and high dosage can promote the absorption and use of the calcium and increase BMD in ovariectomized rats,so it has significant antagonistic effect on osteoporosis.Meanwhile,AOGU CAPSULE also has the effect of inhibiting the increase of body mass caused by the lower level of estrogen.

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更新日期/Last Update: 2013-01-20