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[1]戴七一,文宗振,辛?xí)詵|,等.逍遙散對雌性兔膝關(guān)節(jié)軟骨細胞超微結(jié)構(gòu)的影響[J].中醫(yī)正骨,2012,24(09):8-10.
　DAI Qi-yi*,WEN Zong-zhen,XIN Xiao-dong,et al.Study on the effect of XIAOYAO POWDERS on ultrastructure of knee-joint chondrocytes in female rabbits[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(09):8-10.
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逍遙散對雌性兔膝關(guān)節(jié)軟骨細胞超微結(jié)構(gòu)的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期數(shù):
2012年09期
頁碼:
8-10
欄目:
基礎(chǔ)研究
出版日期:
2012-09-30

文章信息/Info

Title:
Study on the effect of XIAOYAO POWDERS on ultrastructure of knee-joint chondrocytes in female rabbits
作者:
戴七一1; 文宗振2; 辛?xí)詵|1; 覃學(xué)流1; 劉靖3; 騰居贊1;  朱江龍1; 韓杰1; 歐國建1; 袁經(jīng)陽1; 郝高庭1
1.廣西中醫(yī)學(xué)院附屬瑞康醫(yī)院,廣西 南寧 530011;
2.廣西大學(xué),廣西 南寧 530004;
3.廣西中醫(yī)學(xué)院,廣西 南寧 530001
Author(s):
DAI Qi-yi*; WEN Zong-zhen; XIN Xiao-dong; QIN Xue-liu; LIU Jing; TENG Ju-zan; ZHU Jiang-long; HAN Jie; OU Guo-jian; YUAN Jing-yang; HAO Gao-ting.*
Ruikang Hospital affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530011,Guangxi,China
關(guān)鍵詞:
骨關(guān)節(jié)炎; 膝 逍遙散 軟骨細胞 兔 動物實驗
Keywords:
Osteoarthritis; Knee;  XIAOYAO POWDERS;  Chondrocytes;  Rabbits;  Animal experimentation
摘要:
目的:探討逍遙散對雌性兔膝關(guān)節(jié)軟骨細胞超微結(jié)構(gòu)的影響。方法:采用改良Huith法對30只雌性新西蘭兔右膝關(guān)節(jié)進行膝骨關(guān)節(jié)炎造模,造模1周后將30只實驗兔隨機分為逍遙丸組、玻璃酸鈉組及對照組,每組10只。逍遙丸組每天每只給予8丸逍遙丸(相當于原藥材3 g),配成30 mL液體灌胃,連用5周; 玻璃酸鈉組予以玻璃酸鈉注射液0.15 mL·kg-1右膝關(guān)節(jié)內(nèi)注射,每周1次,連用5周; 對照組每天予以30 mL生理鹽水灌胃,連用5周。造模后12周末處死所有實驗兔,取右膝內(nèi)側(cè)脛骨平臺軟骨組織切片后透射電鏡觀察。結(jié)果:逍遙丸組細胞核正常,核膜完整,核質(zhì)均勻,線粒體嵴部分模糊,出現(xiàn)空泡化現(xiàn)象,內(nèi)質(zhì)網(wǎng)、高爾基體正常,纖維組織稍紊亂,有凋亡小體存在; 玻璃酸鈉組細胞核正常,核膜完整,核質(zhì)均勻,線粒體空泡化較輕,內(nèi)質(zhì)網(wǎng)、高爾基體正常,纖維組織稍紊亂,有凋亡小體存在; 對照組細胞核可見固縮現(xiàn)象,線粒體異常,表現(xiàn)為線粒體嵴模糊、消失,空泡化現(xiàn)象較明顯,內(nèi)質(zhì)網(wǎng)、高爾基體基本正常,纖維組織紊亂現(xiàn)象較明顯,多個標本見凋亡小體。結(jié)論:逍遙散對雌性兔膝關(guān)節(jié)軟骨細胞具有保護作用。
Abstract:
Objective:To explore the effect of XIAOYAO POWDERS on the ultrastructure of knee-joint chondrocytes in female rabbits.Methods:Models of knee osteoarthritis(KOA)were built in right knee joints of 30 female New Zealand rabbits by the improved Huith method.One week after modeling,30 experimental rabbits were randomly divided into XIAOYAO POWDERS group,sodium hyaluronate group and control group,10 cases in each group.Every rabbit in the XIAOYAO POWDERS group was intragastric administrated with 30 mL liquid which made from 8 XIAOYAO POWDERS(which dosage was equivalent to that of 3 g original medicine)every day for consecutive 5 weeks.Rabbits in the sodium hyaluronate group were injected with 0.15 mL·kg-1 sodium hyaluronate injection in their right knee joints once a week for consecutive 5 weeks,while the others in the control group were intragastric administrated with 30 mL normal saline every day for consecutive 5 weeks.At the end of 12 weeks after modeling,all the experimental rabbits were executed,and sections from the tibial plateau cartilage tissue inside of their right knees were fetched out to be observed under transmission electron microscope.Results:Such situations were found in XIAOYAO POWDERS group as normal cell nucleus,complete nuclear membrane,uniform nucleoplasm,partially obscuring in mitochondrial crista,vacuolation,normal endoplasmic reticulum and Golgi's apparatus,slightly disorder in fibrous tissue and existence of apoptotic bodies.Such phenomenons were found in sodium hyaluronate group as normal cell nucleus,complete nuclear membrane,uniform nucleoplasm,lesser vacuolation of mitochondria,normal endoplasmic reticulum and Golgi's apparatus,slightly disorder in fibrous tissue and existence of apoptotic bodies.The conditions appeared in a number of specimens of control group as pycnosis of cell nucleus,abnormal mitochondria shown as blurred or disappeared mitochondrial crista,much more obviously vacuolation of mitochondria,essentially normal endoplasmic reticulum and Golgi's apparatus,much more obviously disorder in fibrous tissue and apoptotic bodies.Conclusion:XIAOYAO POWDERS has protective effect on knee-joint chondrocytes in female rabbits.

參考文獻/References:

[1] 任海龍,邢國勝,白人驍.雌激素與骨性關(guān)節(jié)炎[J].中國骨傷,2005,18(12):766-768.
[2] 王澤華.婦產(chǎn)科治療學(xué)[M].北京:人民衛(wèi)生出版社,2009:734-741.
[3] 劉獻祥,李西海,周江濤.改良Hulth造模法復(fù)制膝骨性關(guān)節(jié)炎的實驗研究[J].中國中西醫(yī)結(jié)合雜志,2005,25(12):1104-1108.
[4] 施桂英.關(guān)節(jié)炎概要[M].北京:中國醫(yī)藥科技出版社,2002:327.
[5] Buckwalter JA,Einhorn TA,Simon SR.骨科基礎(chǔ)科學(xué)[M].陳啟明,梁國穗,秦嶺,等譯.北京:人民衛(wèi)生出版社,2001.
[6] 戴七一,劉百玲,韋家寧,等.膝骨性關(guān)節(jié)炎的分型與性別、年齡的關(guān)系[J].中醫(yī)正骨,2007,19(10):12-13.
[7] 代嶺輝,杜寧.關(guān)節(jié)軟骨損傷生物學(xué)修復(fù)的研究進展[J].中國骨傷,2009,22(9):721-724.
[8] Rossouw JE,Anderson GL,Prentice RL,et al.Risks and benefits of estrogen plus progestin in healthy postmenopausal women:principal results From the Women's Health Initiative randomized controlled trial[J].JAMA,2002,288(3):321-333.

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備注/Memo

備注/Memo:
2010-08-21收稿 2012-08-29修回
基金項目:廣西區(qū)衛(wèi)生廳重點課題資助項目(重200856)
通訊作者:覃學(xué)流 E-mail:[email protected]
更新日期/Last Update: 2012-09-30