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[1]姜?jiǎng)磐?安文博.腰腿痛膠囊對髓核移植型腰椎間盤突出癥大鼠模型背根神經(jīng)節(jié)中P物質(zhì)和降鈣素基因相關(guān)肽含量的影響[J].中醫(yī)正骨,2012,24(06):18-20.
　JIANG Jin-ting*,AN Wen-bo.*.Effect of capsules for lumbago-leg pain on the contents of substance P and calcitonin gene-related peptide in dorsal root ganglia of rats models with lumbar disc herniation in nucleus pulposus transplantation type[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(06):18-20.
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腰腿痛膠囊對髓核移植型腰椎間盤突出癥大鼠模型背根神經(jīng)節(jié)中P物質(zhì)和降鈣素基因相關(guān)肽含量的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期數(shù):
2012年06期
頁碼:
18-20
欄目:
腰椎間盤突出癥
出版日期:
2012-06-30

文章信息/Info

Title:
Effect of capsules for lumbago-leg pain on the contents of substance P and calcitonin gene-related peptide in dorsal root ganglia of rats models with lumbar disc herniation in nucleus pulposus transplantation type
作者:
安文博2
1.甘肅中醫(yī)學(xué)院附屬醫(yī)院，甘肅蘭州730020；
2.甘肅中醫(yī)學(xué)院2009級碩士研究生，甘肅蘭州730000
Author(s):
JIANG Jin-ting*; AN Wen-bo.*
University Hospital of Gansu Traditional Chinese Medicine,Lanzhou 730000,Gansu,China
關(guān)鍵詞:
腰腿痛　椎間盤移位　腰椎　神經(jīng)節(jié); 脊　Ｐ物質(zhì)　降鈣素基因相關(guān)肽　動(dòng)物實(shí)驗(yàn)
Keywords:
Lumbago-leg pain; Intervertebral disk displacement; Lumbar vertebrae; Ganglia; spinal; Substance P;  Calcitonin gene-related peptide; Animal experimentation
摘要:
目的：觀察腰腿痛膠囊對髓核移植型腰椎間盤突出癥大鼠模型背根神經(jīng)節(jié)中P物質(zhì)和降鈣素基因相關(guān)肽含量的影響，探討腰 腿痛膠囊治療腰椎間盤突出癥的作用機(jī)理。方法：將48只成年SD大鼠隨機(jī)分為空白對照組、模型組、中藥對照組及腰腿痛膠囊 組，每組12只。采用髓核移植的造模方法將除空白對照組外的其余各組大鼠制成腰椎間盤突出癥大鼠模型。自造模后第2天開始 ，腰腿痛膠囊組每日灌服腰腿痛膠囊與生理鹽水配置的懸濁液，中藥對照組每天灌服腰腿痛丸與生理鹽水配置的懸濁液，空白 對照組和模型組灌服等體積的生理鹽水。連續(xù)灌胃2周后，取各組大鼠尾段髓核移植于L4～L6鄰近支脊神經(jīng)背根神經(jīng)節(jié)，采 用免疫組化法測定各組大鼠背根神經(jīng)節(jié)中P物質(zhì)和降鈣素基因相關(guān)肽的含量。結(jié)果：①P物質(zhì)平均灰度。4組大鼠P物質(zhì)平均灰度 比較，差異有統(tǒng)計(jì)學(xué)意義（F=19.912，P=0.000）。進(jìn)一步兩兩比較，模型組平均灰度大于空白對照組、中藥對照組及腰腿 痛膠囊組（P=0.000；P=0.000；P=0.000）；腰腿痛膠囊組平均灰度小于中藥對照組（P=0.000）。②降鈣素基因相關(guān) 肽平均灰度。4組大鼠降鈣素基因相關(guān)肽平均灰度比較，差異有統(tǒng)計(jì)學(xué)意義（F=16.367，P=0.000）。進(jìn)一步兩兩比較，模 型組平均灰度大于空白對照組（P=0.000）；腰腿痛膠囊組與模型組比較，差異無統(tǒng)計(jì)學(xué)意義（P=0.749）；中藥對照 組大于模型組和腰腿痛膠囊組（P=0.033，P=0.000）。結(jié)論：腰腿痛膠囊通過降低實(shí)驗(yàn)性腰椎間盤突出癥大鼠背根神經(jīng)節(jié) 中P物質(zhì)含量，減輕由髓核組織對背根神經(jīng)節(jié)的損傷和刺激而引起的疼痛，這可能是腰腿痛膠囊治療腰椎間盤突出癥的作用機(jī)理 之一。
Abstract:
Objective:To observe the effect of capsules for lumbago-leg pain(CLLP)on the contents of substance P(SP)and calcitonin gene-related peptide(CGRP)in dorsal root ganglia of rats models with lumbar disc herniation(LDH)in nucleus pulposus transplantation type,and to explore the mechanism of capsules for lumbago-leg pain in the treatment of LDH.Methods:Forty-eight adult SD rats were randomly divided into blank control group,model group,traditional Chinese medicine(TCM)control group and CLLP group,12 cases in each group.All the rats were administrated with modeling method of nucleus pulposus transplantation to build LDH rats models except those in the blank control group.From the second day after modeling,rats in CLLP group were administrated with suspension composed of CLLP and normal saline per day,cases in TCM control group were administrated with suspension composed of pills for lumbago-leg pain and normal saline per day,while the others in blank control group and model group were administrated with normal saline in the same volumes.After continious 2 weeks of intragastric administration,caudal nucleus pulposus of rats were transplanted in dorsal root ganglia of spinal nerve near the L4～L6,then SP and CGRP contents in dorsal root ganglia for all the rats were measured through using immunohistochemistry.Results:There were statistical differences in average gray scale of SP among the 4 groups(F=19.912,P=0.000).Average gray scale of SP in model group was higher than that of blank control group,TCM control group and CLLP group respectively (P=0.000;P=0.000;P=0.000);and the average gray scale of SP in CLLP group was lower than that of TCM control group(P=0.000).There were statistical differences in average gray scale of CGRP among the 4 groups( F=16.367,P=0.000).Average gray scale of CGRP of model group was 〖HJ*6〗 基金項(xiàng)目：蘭州市科技計(jì)劃項(xiàng)目（2004-1-62） 通訊作者：安文博E-mail：[email protected] higher than that of blank control group(P=0.000);and there was no statistical difference between CLLP group and model group(P=0.749);and the average gray scale of CGRP of TCM control group was higher than that of model group and CLLP group respectively(P=0.033,P=0.000).Conclusion:The capsule for lumbago-leg pain can relieve the pain caused by the injuries and stimulations of dorsal root ganglia from nucleus pulposus through reducing SP contents in dorsal root ganglia of experimental LDH rats.It may be one of the mechanisms of action of the capsule for lumbago-leg pain in the treatment of LDH.

參考文獻(xiàn)/References:

[1]魯玉來,蔡欽林.腰椎間盤突出癥[M].北京:人民軍醫(yī)出版社,2001:189-193.
[2]岳壽偉,劉慶.腰腿痛發(fā)病機(jī)制研究[J].中國臨床康復(fù),2002,6(12):1724-1725,1733.
[3]姜?jiǎng)磐?腰腿痛膠囊對髓核移植型腰椎間盤突出癥大鼠模型背根節(jié)及坐骨神經(jīng)中SP和CGRP的影響[D].蘭州:甘肅中醫(yī)學(xué) 院,2005.
[4]韓學(xué)哲,劉淼,王民,等.大鼠腰椎P物質(zhì)和降鈣素基因相關(guān)肽能神經(jīng)纖維的分布[J].頸腰痛雜志,2002,23(1):25-27.
[5]周知,李元建.降鈣素基因相關(guān)肽抗高血壓內(nèi)皮祖細(xì)胞衰老作用和機(jī)制的研究[D].長沙:中南大學(xué),2009.
[6]關(guān)新民.醫(yī)學(xué)神經(jīng)生物學(xué)[M].北京:人民衛(wèi)生出版社,2002:157-163.
[7]沈?yàn)闂?李康華.腰椎間盤突出癥患者手術(shù)前后血漿內(nèi)皮素及降鈣素基因相關(guān)肽的變化[J].中華實(shí)驗(yàn)外科雜志,2003,20 (11):1041-1042.

備注/Memo

備注/Memo:
2011-03-13收稿2011-05-25修回
更新日期/Last Update: 2012-06-30