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[1]周江濤,王慶來,趙依娜,等.獨(dú)活寄生湯對腰椎間盤纖維環(huán)細(xì)胞P38信號轉(zhuǎn)導(dǎo)通路的影響[J].中醫(yī)正骨,2012,24(06):9-12.
　ZHOU Jiang-tao*,WANG Qing-lai,ZHAO Yi-na,et al.Empirical study of the effect of DUHUO JISHENG decoction on p38 signal transduction pathway in annulus fibrosus cells isolated from lumbar intervertebral disc[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(06):9-12.
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獨(dú)活寄生湯對腰椎間盤纖維環(huán)細(xì)胞P38信號轉(zhuǎn)導(dǎo)通路的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期數(shù):
2012年06期
頁碼:
9-12
欄目:
腰椎間盤突出癥
出版日期:
2012-06-30

文章信息/Info

Title:
Empirical study of the effect of DUHUO JISHENG decoction on p38 signal transduction pathway in annulus fibrosus cells isolated from lumbar intervertebral disc
作者:
周江濤1; 王慶來1; 趙依娜1; 吳惠明1; 王維佳2; 王鵬2
1.浙江省溫州市中醫(yī)院，浙江溫州325000；
2.浙江中醫(yī)藥大學(xué)，浙江杭州310053
Author(s):
ZHOU Jiang-tao*; WANG Qing-lai; ZHAO Yi-na; WU Hui-ming; WANG Wei- jia; WANG Peng.*
Traditional Chinese Medical Hospital of Wenzhou City,Wenzhou 325000,Zhejiang,China
關(guān)鍵詞:
腰痛　椎間盤　獨(dú)活寄生湯　Ｐ３８絲裂原活化蛋白激酶類　動(dòng)物實(shí)驗(yàn)
Keywords:
Low back pain; Intervertebral disk; DUHUO JISHENG decoction; p38 mitogen-activated protein kinases;  Animal experimentation
摘要:
從P38細(xì)胞信號轉(zhuǎn)導(dǎo)通路的角度探討?yīng)毣罴纳鷾委熝甸g盤源性腰痛的作用機(jī)制。方法：將20只3月齡雄性新西蘭 大白兔隨機(jī)分為正常對照組、模型組、獨(dú)活寄生湯組及P38阻斷劑組，每組5只。除正常對照組外，其余3組均采用纖維環(huán)體表穿 刺法建立兔腰椎間盤退變模型。造模8 d后提取各組實(shí)驗(yàn)兔含藥血清低溫保存?zhèn)溆茫谠炷?周后分別用這些血清刺激培養(yǎng)的椎 間盤纖維環(huán)細(xì)胞，并采用Western Blot法檢測各組細(xì)胞的P38絲裂原活化蛋白激酶磷酸化水平。結(jié)果：各組纖維環(huán)細(xì)胞P38絲裂 原活化蛋白激酶總量比較，差異無統(tǒng)計(jì)學(xué)意義（F=1.819，P=0.085）。各組纖維環(huán)細(xì)胞磷酸化P38絲裂原活化蛋白激酶/P38 絲裂原活化蛋白激酶總量比較，差異有統(tǒng)計(jì)學(xué)意義（F=5.437，P=0.007），進(jìn)一步兩兩比較：模型組大于獨(dú)活寄生湯組、 P38阻斷劑組及正常對照組（P=0.009，P=0.005，P=0.003）；獨(dú)活寄生湯組大于P38阻斷劑組及正常對照組（P=0.007，P =0.004）；P38阻斷劑組大于正常對照組（P=0.007）。結(jié)論：獨(dú)活寄生湯可通過降低纖維環(huán)細(xì)胞P38絲裂原活化蛋白 激酶磷酸化水平，抑制炎性因子的產(chǎn)生，減緩由P38絲裂原活化蛋白激酶信號轉(zhuǎn)導(dǎo)通路介導(dǎo)的軟骨細(xì)胞凋亡進(jìn)程，從而達(dá)到治療 腰椎間盤源性腰痛的目的。
Abstract:
Objective:To explore the mechanism of action of DUHUO JISHENG decoction in the treatment of low back pain caused by intervertebral disk disease from the standpoint of p38 signal transduction pathway.Methods:Twenty male New Zealand white rabbits,3 months old,were randomly divided into four groups,5 cases in each group:normal control group-normal saline(7 ml/kg/day gavage);model group-normal saline(7 ml/kg/day gavage);DUHUO JISHENG decoction group-DUHUO JISHENG decoction(7 ml/kg/day gavage);p38 blocking agent group-p38 blocking agent(7 ml/kg/day gavage).The rabbit models of lumbar intervertebral disk cataplasia were built by puncturing the annular fibrosus through body surface except the normal control group.Eight days after modeling,blood sera containing durg of rabbits were extracted and preserved in low temperature.Eight weeks after modeling,the annulus fibrosus cells isolated from lumbar intervertebral disc were stimulated with the blood sera containing drug,and the phosphorylation level of p38 mitogen-activated protein kinases(MAPK)in cells were detected by western blot.Results:There were no statistical differences in the total amount of p38 MAPK in annulus fibrosus cells among these groups(F=1.819,P=0.085).There were statistical differences in the ratio of phosphorylated p38 MAPK to total p38 MAPK in annulus fibrosus cells among these groups (F=5.437,P=0.007);further comparison indicated that the ratio of model group was higher than that of DUHUO JISHENG decoction group,p38 blocking agent group and normal control group(P=0.009,P=0.005,P=0.003),the ratio of DUHUO JISHENG decoction group was higher than that of p38 blocking agent group and normal control group(P=0.007,P=0.004),the ratio of p38 blocking agent group was higher than that of normal control group( P=0.007).Conclusion:DUHUO JISHENG decoction can inhibit the production of inflammatory factors and slow down the process of apoptosis of cartilage cells induced by p38 MAPK signal transduction pathway by depressing the phosphorylation of p38 MAPK of annulus fibrosus cells,for the purpose of treatment of low back pain caused by intervertebral disk disease.

參考文獻(xiàn)/References:

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備注/Memo

備注/Memo:
2011-11-06收稿2012-04-05修回
基金項(xiàng)目：浙江省中醫(yī)藥科技項(xiàng)目（2008CA103）
更新日期/Last Update: 2012-06-30