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[1]譚勇海,姜苗苗,韓海霞,等.陶瓷化骨對成人骨髓基質(zhì)干細(xì)胞分化的影響[J].中醫(yī)正骨,2012,24(05):23-26.
　TAN Yong-hai*,JIANG Miao-miao,HAN Hai-xia,et al.The effect of ceramic bovine bone on growth and differentiation of adult human bone marrow stromal cells[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(05):23-26.
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陶瓷化骨對成人骨髓基質(zhì)干細(xì)胞分化的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期數(shù):
2012年05期
頁碼:
23-26
欄目:
基礎(chǔ)研究
出版日期:
2012-05-20

文章信息/Info

Title:
The effect of ceramic bovine bone on growth and differentiation of adult human bone marrow stromal cells
作者:
譚勇海; 姜苗苗; 韓海霞; 于海勇
山東省文登整骨醫(yī)院,山東 文登 264400
Author(s):
TAN Yong-hai*; JIANG Miao-miao; HAN Hai-xia; YU Hai-yong.
*Wendeng Orthopaedic Hospital,Wendeng 264400,Shandong,China
關(guān)鍵詞:
陶瓷化骨 骨髓基質(zhì)干細(xì)胞 分化
Keywords:
Ceramic bovine bon;  Mesenchymal stem cells;  Differentiation
摘要:
目的:觀察陶瓷化骨對成人骨髓基質(zhì)干細(xì)胞生長和分化的影響。方法:體外分離培養(yǎng)成人骨髓基質(zhì)干細(xì)胞,將第二代細(xì)胞以陶瓷化骨為載體進(jìn)行培養(yǎng),利用倒置顯微鏡、堿性磷酸酶細(xì)胞化學(xué)染色、堿性磷酸酶活性檢測等條件,了解陶瓷化骨對成人骨髓基質(zhì)干細(xì)胞生物學(xué)行為的影響。結(jié)果:體外連續(xù)培養(yǎng)6 d,倒置顯微鏡下可見成人骨髓基質(zhì)干細(xì)胞與陶瓷化骨相容性好,材料周邊細(xì)胞生長密集,并有向陶瓷化骨趨化遷移現(xiàn)象,3周后形成礦化結(jié)節(jié)。堿性磷酸酶染色呈陽性、堿性磷酸酶活性檢測有明顯的細(xì)胞分泌量。結(jié)論:體外培養(yǎng)條件下,陶瓷化骨可誘導(dǎo)成人骨髓基質(zhì)干細(xì)胞向成骨和骨質(zhì)細(xì)胞分化。
Abstract:
Objective:To observe the effect of ceramic bovine bone(CBB)on growth and differentiation of adult human bone marrow stromal cells(hBMSCs)in vitro.Methods:The hBMSCs were cultured in vitro,the second generation of cells were cultured with CBB as the carrier,inverted microscope,alkaline phosphatase(AKP)staining,ALP activity detection conditions were used to investigate effect of CBB on hBMSCs biological behavior.Results:After continuous cultivation in vitro for 6 Days, hBMSCs and CBB showed good compatibility under inverted microscope,the cells were densely distributed around the material and showed CBB chemotactic migration,the formation of mineralized nodules.AKP staining showed positive and significant secretion was detected.Conclusion:CBB could induced hBMSCs to differentiated into osteoblasts and bone cells in vitro conditions.

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更新日期/Last Update: 2012-05-20